LOCUS       RC230704                8901 bp    DNA     circular  SYN 
DEFINITION  PARD3 (Myc-DDK-tagged)-Human par-3 partitioning defective 3 homolog (C. elegans) (PARD3), transcript variant 3
ACCESSION   NM_001184786
VERSION     NM_001184786.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5058..5149 
                     /label="Myc-DDK"  
     misc_feature    5179..5764 
                     /label="PolyA signal"  
     misc_feature    5236..5255 
                     /label="Restriction Enzyme"  
     misc_feature    6040..6712 
                     /label="ColE1"  
     misc_feature    7142..7936 
                     /label="Kan/Neo"  
     misc_feature    7971..8273 
                     /label="SV40 ori"  
     misc_feature    8359..8836 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5040..5046 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5040 
                     /label="PARD3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGACC GTGTGCTTCG GACGGACCCG GGTGGTCGTG CCGTGCGGGG 
     1081 ACGGCCACAT GAAAGTTTTC AGCCTCATCC AGCAGGCGGT GACCCGCTAC CGGAAGGCCA 
     1141 TCGCCAAGGA TCCAAACTAC TGGATACAGG TGCATCGCTT GGAACATGGA GATGGAGGAA 
     1201 TACTAGACCT TGATGACATT CTTTGTGATG TAGCAGACGA TAAAGACAGA CTGGTAGCAG 
     1261 TGTTTGATGA GCAGGATCCA CATCACGGAG GTGATGGCAC CAGTGCCAGT TCCACGGGTA 
     1321 CCCAGAGCCC AGAGATATTT GGTAGTGAGC TTGGCACCAA CAATGTCTCA GCCTTTCAGC 
     1381 CTTACCAAGC AACAAGTGAA ATTGAGGTCA CACCTTCAGT CCTTCGAGCA AATATGCCTC 
     1441 TTCATGTTCG ACGCAGTAGT GACCCAGCTC TAATTGGCCT CTCCACTTCT GTCAGTGATA 
     1501 GTAATTTTTC CTCTGAAGAG CCTTCAAGGA AAAATCCCAC ACGCTGGTCA ACAACAGCTG 
     1561 GCTTCCTCAA GCAGAACACT GCTGGGAGTC CTAAAACCTG CGACAGGAAG AAAGATGAAA 
     1621 ACTACAGAAG CCTCCCGCGG GATACTAGTA ACTGGTCTAA CCAATTTCAG AGAGACAATG 
     1681 CTCGCTCGTC TCTGAGTGCC AGTCACCCAA TGGTGGGCAA GTGGCTGGAG AAACAAGAAC 
     1741 AGGATGAGGA TGGGACAGAA GAGGATAACA GTCGTGTTGA ACCTGTTGGA CATGCTGACA 
     1801 CGGGTTTGGA GCATATACCC AACTTTTCTC TGGATGATAT GGTAAAGCTC GTAGAAGTCC 
     1861 CCAACGATGG AGGGCCTCTG GGAATCCATG TAGTGCCTTT CAGTGCTCGA GGCGGCAGAA 
     1921 CCCTGGGGTT ATTAGTAAAA CGATTGGAGA AAGGTGGTAA AGCTGAACAT GAAAATCTTT 
     1981 TTCGTGAGAA TGATTGCATT GTCAGGATTA ATGATGGCGA CCTTCGAAAT AGAAGATTTG 
     2041 AACAAGCACA ACATATGTTT CGCCAAGCCA TGCGTACACC CATCATTTGG TTCCATGTGG 
     2101 TTCCTGCAGC AAATAAAGAG CAGTATGAAC AACTATCCCA AAGTGAGAAG AACAATTACT 
     2161 ATTCAAGCCG TTTTAGCCCT GACAGCCAGT ATATTGACAA CAGGAGTGTG AACAGTGCAG 
     2221 GGCTTCACAC GGTGCAGAGA GCACCCCGAC TGAACCACCC GCCTGAGCAG ATAGACTCTC 
     2281 ACTCAAGACT ACCTCATAGC GCACACCCCT CGGGAAAACC ACCATCCGCT CCAGCCTCGG 
     2341 CACCTCAGAA TGTATTTAGT ACGACTGTAA GCAGTGGTTA TAACACCAAA AAAATAGGCA 
     2401 AGAGGCTTAA TATCCAGCTT AAGAAAGGTA CAGAAGGTTT GGGATTCAGC ATCACTTCCA 
     2461 GAGATGTAAC AATAGGTGGC TCAGCTCCAA TCTATGTGAA AAACATTCTC CCCCGGGGGG 
     2521 CGGCCATTCA GGATGGCCGA CTTAAGGCAG GAGACAGACT TATAGAGGTA AATGGAGTAG 
     2581 ATTTAGTGGG CAAATCCCAA GAGGAAGTTG TTTCGCTGTT GAGAAGCACC AAGATGGAAG 
     2641 GAACTGTGAG CCTTCTGGTC TTTCGCCAGG AAGACGCCTT CCACCCAAGG GAACTGAAAG 
     2701 CAGAAGATGA GGATATTGTT CTTACACCTG ATGGCACCAG GGAATTTCTG ACATTTGAAG 
     2761 TCCCACTTAA TGATTCAGGA TCTGCAGGCC TTGGTGTCAG TGTCAAAGGT AACCGGTCAA 
     2821 AAGAGAACCA CGCAGATTTG GGAATCTTTG TCAAGTCCAT TATTAATGGA GGAGCAGCAT 
     2881 CTAAAGATGG AAGGCTTCGG GTGAATGATC AACTGATAGC AGTAAATGGA GAATCCCTGT 
     2941 TGGGCAAGAC AAACCAAGAT GCCATGGAAA CCCTAAGAAG GTCTATGTCT ACTGAAGGCA 
     3001 ATAAACGAGG AATGATCCAG CTTATTGTTG CAAGGAGAAT AAGCAAGTGC AATGAGCTGA 
     3061 AGTCACCTGG GAGCCCCCCT GGACCTGAGC TGCCCATTGA AACAGCGTTG GATGATAGAG 
     3121 AACGAAGAAT TTCCCATTCC CTCTACAGTG GGATTGAGGG GCTTGATGAA TCGCCCAGCA 
     3181 GAAATGCTGC CCTCAGTAGG ATAATGGGTA AATACCAGCT GTCCCCTACA GTGAATATGC 
     3241 CCCAAGATGA CACTGTCATT ATAGAAGATG ACAGGTTGCC AGTGCTTCCT CCACATCTCT 
     3301 CTGACCAGTC CTCTTCCAGC TCCCATGATG ATGTGGGGTT TGTGACGGCA GATGCTGGTA 
     3361 CTTGGGCCAA GGCTGCAATC AGTGATTCAG CCGACTGCTC TTTGAGTCCA GATGTTGATC 
     3421 CAGTTCTTGC TTTTCAACGA GAAGGATTTG GACGTCAGAG TATGTCAGAA AAACGCACAA 
     3481 AGCAATTTTC AGATGCCAGT CAATTGGATT TCGTTAAAAC ACGAAAATCA AAAAGCATGG 
     3541 ATTTAGGTAT AGCTGACGAG ACTAAACTCA ATACAGTGGA TGACCAGAAA GCAGGTTCTC 
     3601 CCAGCAGAGA TGTGGGTCCT TCCCTGGGTC TGAAGAAGTC AAGCTCGTTG GAGAGTCTGC 
     3661 AGACCGCAGT TGCCGAGGTG ACTTTGAATG GGGATATTCC TTTCCATCGT CCACGGCCGC 
     3721 GGATAATCAG AGGCAGGGGA TGCAATGAGA GCTTCAGAGC TGCCATCGAC AAATCTTATG 
     3781 ATAAACCCGC GGTAGATGAT GATGATGAAG GCATGGAGAC CTTGGAAGAA GACACAGAAG 
     3841 AAAGTTCAAG ATCAGGGAGA GAGTCTGTAT CCACAGCCAG TGATCAGCCT TCCCACTCTC 
     3901 TGGAGAGACA AATGAATGGA AACCAAGAGA AAGGTGATAA GACTGATAGA AAAAAGGATA 
     3961 AAACTGGAAA AGAAAAGAAG AAAGATAGAG ATAAGGAGAA GGATAAAATG AAAGCCAAGA 
     4021 AGGGAATGCT GAAGGGCTTG GGAGACATGT TCAGGTTTGG CAAACATCGA AAAGATGACA 
     4081 AGATTGAGAA AACGGGTAAA ATAAAAATAC AGGAATCCTT TACATCAGAA GAGGAGAGGA 
     4141 TACGAATGAA GCAGGAGCAG GAGAGGATTC AAGCCAAAAC TCGAGAATTT AGGGAACGAC 
     4201 AAGCTCGAGA GCGTGACTAT GCTGAAATTC AAGATTTTCA TCGGACATTT GGCTGTGATG 
     4261 ATGAGTTAAT GTATGGGGGA GTTTCTTCTT ATGAAGGTTC CATGGCTCTC AACGCTAGAC 
     4321 CTCAGAGCCC ACGAGAAGGG CATATGATGG ATGCTTTGTA TGCCCAAGTC AAGAAGCCGC 
     4381 GGAATTCCAA ACCCTCACCT GTAGACAGTA ACAGATCAAC TCCTAGCAAT CATGATCGGA 
     4441 TACAGCGTCT GAGGCAAGAA TTTCAGCAAG CAAAGCAAGA TGAAGATGTA GAAGATCGTC 
     4501 GGCGGACCTA TAGTTTTGAG CAACCCTGGC CGAACGCACG GCCGGCGACG CAGAGCGGGC 
     4561 GACACTCGGT GTCCGTGGAG GTGCAGATGC AGCGGCAGCG GCAGGAGGAG CGCGAGAGCT 
     4621 CCCAGCAGGC CCAGCGCCAG TACAGCTCTC TGCCTCGGCA AAGCAGGAAA AATGCCAGCT 
     4681 CGGTCTCCCA GGACTCTTGG GAGCAGAACT ACTCCCCTGG GGAAGGCTTC CAGAGTGCCA 
     4741 AAGAGAACCC CAGGTACTCC AGCTACCAAG GCTCCAGGAA CGGCTACCTG GGAGGACATG 
     4801 GCTTCAACGC CAGGGTCATG CTGGAAACTC AGGAGCTCCT TCGCCAGGAA CAGAGGCGGA 
     4861 AGGAGCAGCA GATGAAGAAG CAGCCTCCTT CCGAGGGGCC CAGCAACTAT GACTCGTATA 
     4921 AGAAAGTCCA GGACCCCAGT TACGCCCCTC CCAAGGGGCC CTTCCGGCAA GATGTGCCCC 
     4981 CCTCCCCTTC TCAGGTTGCG AGGCTGAACA GACTTCAGAC TCCTGAGAAA GGGAGGCCCT 
     5041 TCTATTCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5101 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5161 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5221 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5281 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5341 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5401 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5461 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5521 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5581 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5641 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5701 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5761 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5821 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5881 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5941 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6001 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6061 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6121 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6181 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6241 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6301 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6361 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6421 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6481 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6541 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6601 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6661 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6721 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6781 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6841 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6901 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6961 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7021 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7081 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7141 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7201 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7261 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7321 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7381 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7441 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7501 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7561 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7621 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7681 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7741 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7801 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7861 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7921 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7981 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8041 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8101 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8161 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8221 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8281 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8341 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8401 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8461 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8521 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8581 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8641 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8701 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8761 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8821 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8881 CAAAAATTTA ACGCGAATTT T
//