LOCUS       RC230699                8838 bp    DNA     circular  SYN 
DEFINITION  PARD3 (Myc-DDK-tagged)-Human par-3 partitioning defective 3 homolog (C. elegans) (PARD3), transcript variant 4
ACCESSION   NM_001184787
VERSION     NM_001184787.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4995..5086 
                     /label="Myc-DDK"  
     misc_feature    5116..5701 
                     /label="PolyA signal"  
     misc_feature    5173..5192 
                     /label="Restriction Enzyme"  
     misc_feature    5977..6649 
                     /label="ColE1"  
     misc_feature    7079..7873 
                     /label="Kan/Neo"  
     misc_feature    7908..8210 
                     /label="SV40 ori"  
     misc_feature    8296..8773 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4977..4983 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4977 
                     /label="PARD3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGACC GTGTGCTTCG GACGGACCCG GGTGGTCGTG CCGTGCGGGG 
     1081 ACGGCCACAT GAAAGTTTTC AGCCTCATCC AGCAGGCGGT GACCCGCTAC CGGAAGGCCA 
     1141 TCGCCAAGGA TCCAAACTAC TGGATACAGG TGCATCGCTT GGAACATGGA GATGGAGGAA 
     1201 TACTAGACCT TGATGACATT CTTTGTGATG TAGCAGACGA TAAAGACAGA CTGGTAGCAG 
     1261 TGTTTGATGA GCAGGATCCA CATCACGGAG GTGATGGCAC CAGTGCCAGT TCCACGGGTA 
     1321 CCCAGAGCCC AGAGATATTT GGTAGTGAGC TTGGCACCAA CAATGTCTCA GCCTTTCAGC 
     1381 CTTACCAAGC AACAAGTGAA ATTGAGGTCA CACCTTCAGT CCTTCGAGCA AATATGCCTC 
     1441 TTCATGTTCG ACGCAGTAGT GACCCAGCTC TAATTGGCCT CTCCACTTCT GTCAGTGATA 
     1501 GTAATTTTTC CTCTGAAGAG CCTTCAAGGA AAAATCCCAC ACGCTGGTCA ACAACAGCTG 
     1561 GCTTCCTCAA GCAGAACACT GCTGGGAGTC CTAAAACCTG CGACAGGAAG AAAGATGAAA 
     1621 ACTACAGAAG CCTCCCGCGG GATACTAGTA ACTGGTCTAA CCAATTTCAG AGAGACAATG 
     1681 CTCGCTCGTC TCTGAGTGCC AGTCACCCAA TGGTGGGCAA GTGGCTGGAG AAACAAGAAC 
     1741 AGGATGAGGA TGGGACAGAA GAGGATAACA GTCGTGTTGA ACCTGTTGGA CATGCTGACA 
     1801 CGGGTTTGGA GCATATACCC AACTTTTCTC TGGATGATAT GGTAAAGCTC GTAGAAGTCC 
     1861 CCAACGATGG AGGGCCTCTG GGAATCCATG TAGTGCCTTT CAGTGCTCGA GGCGGCAGAA 
     1921 CCCTGGGGTT ATTAGTAAAA CGATTGGAGA AAGGTGGTAA AGCTGAACAT GAAAATCTTT 
     1981 TTCGTGAGAA TGATTGCATT GTCAGGATTA ATGATGGCGA CCTTCGAAAT AGAAGATTTG 
     2041 AACAAGCACA ACATATGTTT CGCCAAGCCA TGCGTACACC CATCATTTGG TTCCATGTGG 
     2101 TTCCTGCAGC AAATAAAGAG CAGTATGAAC AACTATCCCA AAGTGAGAAG AACAATTACT 
     2161 ATTCAAGCCG TTTTAGCCCT GACAGCCAGT ATATTGACAA CAGGAGTGTG AACAGTGCAG 
     2221 GGCTTCACAC GGTGCAGAGA GCACCCCGAC TGAACCACCC GCCTGAGCAG ATAGACTCTC 
     2281 ACTCAAGACT ACCTCATAGC GCACACCCCT CGGGAAAACC ACCATCCGCT CCAGCCTCGG 
     2341 CACCTCAGAA TGTATTTAGT ACGACTGTAA GCAGTGGTTA TAACACCAAA AAAATAGGCA 
     2401 AGAGGCTTAA TATCCAGCTT AAGAAAGGTA CAGAAGGTTT GGGATTCAGC ATCACTTCCA 
     2461 GAGATGTAAC AATAGGTGGC TCAGCTCCAA TCTATGTGAA AAACATTCTC CCCCGGGGGG 
     2521 CGGCCATTCA GGATGGCCGA CTTAAGGCAG GAGACAGACT TATAGAGGTA AATGGAGTAG 
     2581 ATTTAGTGGG CAAATCCCAA GAGGAAGTTG TTTCGCTGTT GAGAAGCACC AAGATGGAAG 
     2641 GAACTGTGAG CCTTCTGGTC TTTCGCCAGG AAGACGCCTT CCACCCAAGG GAACTGAATG 
     2701 CAGAGCCAAG CCAGATGCAG ATTCCAAAAG AAACGAAAGC AGAAGATGAG GATATTGTTC 
     2761 TTACACCTGA TGGCACCAGG GAATTTCTGA CATTTGAAGT CCCACTTAAT GATTCAGGAT 
     2821 CTGCAGGCCT TGGTGTCAGT GTCAAAGGTA ACCGGTCAAA AGAGAACCAC GCAGATTTGG 
     2881 GAATCTTTGT CAAGTCCATT ATTAATGGAG GAGCAGCATC TAAAGATGGA AGGCTTCGGG 
     2941 TGAATGATCA ACTGATAGCA GTAAATGGAG AATCCCTGTT GGGCAAGACA AACCAAGATG 
     3001 CCATGGAAAC CCTAAGAAGG TCTATGTCTA CTGAAGGCAA TAAACGAGGA ATGATCCAGC 
     3061 TTATTGTTGC AAGGAGAATA AGCAAGTGCA ATGAGCTGAA GTCACCTGGG AGCCCCCCTG 
     3121 GACCTGAGCT GCCCATTGAA ACAGCGTTGG ATGATAGAGA ACGAAGAATT TCCCATTCCC 
     3181 TCTACAGTGG GATTGAGGGG CTTGATGAAT CGCCCAGCAG AAATGCTGCC CTCAGTAGGA 
     3241 TAATGGGTGA GTCAGGTAAA TACCAGCTGT CCCCTACAGT GAATATGCCC CAAGATGACA 
     3301 CTGTCATTAT AGAAGATGAC AGGTTGCCAG TGCTTCCTCC ACATCTCTCT GACCAGTCCT 
     3361 CTTCCAGCTC CCATGATGAT GTGGGGTTTG TGACGGCAGA TGCTGGTACT TGGGCCAAGG 
     3421 CTGCAATCAG TGATTCAGCC GACTGCTCTT TGAGTCCAGA TGTTGATCCA GTTCTTGCTT 
     3481 TTCAACGAGA AGGATTTGGA CGTCAGAGTA TGTCAGAAAA ACGCACAAAG CAATTTTCAG 
     3541 ATGCCAGTCA ATTGGATTTC GTTAAAACAC GAAAATCAAA AAGCATGGAT TTAGGTATAG 
     3601 CTGACGAGAC TAAACTCAAT ACAGTGGATG ACCAGAAAGC AGGTTCTCCC AGCAGAGATG 
     3661 TGGGTCCTTC CCTGGGTCTG AAGAAGTCAA GCTCGTTGGA GAGTCTGCAG ACCGCAGTTG 
     3721 CCGAGGTGAC TTTGAATGGG GATATTCCTT TCCATCGTCC ACGGCCGCGG ATAATCAGAG 
     3781 GCAGGGGATG CAATGAGAGC TTCAGAGCTG CCATCGACAA ATCTTATGAT AAACCCGCGG 
     3841 TAGATGATGA TGATGAAGGC ATGGAGACCT TGGAAGAAGA CACAGAAGAA AGTTCAAGAT 
     3901 CAGGGAGAGA GTCTGTATCC ACAGCCAGTG ATCAGCCTTC CCACTCTCTG GAGAGACAAA 
     3961 TGAATGGAAA CCAAGAGAAA GGTGATAAGA CTGATAGAAA AAAGGATAAA ACTGGAAAAG 
     4021 AAAAGAAGAA AGATAGAGAT AAGGAGAAGG ATAAAATGAA AGCCAAGAAG GGAATGCTGA 
     4081 AGGGCTTGGG AGACATGTTC AGGATTCAAG CCAAAACTCG AGAATTTAGG GAACGACAAG 
     4141 CTCGAGAGCG TGACTATGCT GAAATTCAAG ATTTTCATCG GACATTTGGC TGTGATGATG 
     4201 AGTTAATGTA TGGGGGAGTT TCTTCTTATG AAGGTTCCAT GGCTCTCAAC GCTAGACCTC 
     4261 AGAGCCCACG AGAAGGGCAT ATGATGGATG CTTTGTATGC CCAAGTCAAG AAGCCGCGGA 
     4321 ATTCCAAACC CTCACCTGTA GACAGTAACA GATCAACTCC TAGCAATCAT GATCGGATAC 
     4381 AGCGTCTGAG GCAAGAATTT CAGCAAGCAA AGCAAGATGA AGATGTAGAA GATCGTCGGC 
     4441 GGACCTATAG TTTTGAGCAA CCCTGGCCGA ACGCACGGCC GGCGACGCAG AGCGGGCGAC 
     4501 ACTCGGTGTC CGTGGAGGTG CAGATGCAGC GGCAGCGGCA GGAGGAGCGC GAGAGCTCCC 
     4561 AGCAGGCCCA GCGCCAGTAC AGCTCTCTGC CTCGGCAAAG CAGGAAAAAT GCCAGCTCGG 
     4621 TCTCCCAGGA CTCTTGGGAG CAGAACTACT CCCCTGGGGA AGGCTTCCAG AGTGCCAAAG 
     4681 AGAACCCCAG GTACTCCAGC TACCAAGGCT CCAGGAACGG CTACCTGGGA GGACATGGCT 
     4741 TCAACGCCAG GGTCATGCTG GAAACTCAGG AGCTCCTTCG CCAGGAACAG AGGCGGAAGG 
     4801 AGCAGCAGAT GAAGAAGCAG CCTCCTTCCG AGGGGCCCAG CAACTATGAC TCGTATAAGA 
     4861 AAGTCCAGGA CCCCAGTTAC GCCCCTCCCA AGGGGCCCTT CCGGCAAGAT GTGCCCCCCT 
     4921 CCCCTTCTCA GGTTGCGAGG CTGAACAGAC TTCAGACTCC TGAGAAAGGG AGGCCCTTCT 
     4981 ATTCCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5041 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5101 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5161 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5221 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5281 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5341 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5401 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5461 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5521 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5581 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5641 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5701 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5761 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5821 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5881 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5941 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6001 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6061 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6121 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6181 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6241 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6301 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6361 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6421 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6481 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6541 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6601 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6661 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6721 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6781 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6841 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6901 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6961 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7021 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7081 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7141 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7201 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7261 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7321 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7381 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7441 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7501 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7561 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7621 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7681 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7741 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7801 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7861 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7921 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7981 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8041 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8101 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8161 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8221 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8281 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8341 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8401 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8461 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8521 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8581 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8641 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8701 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8761 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8821 AAATTTAACG CGAATTTT
//