LOCUS       RC230698                8811 bp    DNA     circular  SYN 
DEFINITION  PARD3 (Myc-DDK-tagged)-Human par-3 partitioning defective 3 homolog (C. elegans) (PARD3), transcript variant 5
ACCESSION   NM_001184788
VERSION     NM_001184788.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4968..5059 
                     /label="Myc-DDK"  
     misc_feature    5089..5674 
                     /label="PolyA signal"  
     misc_feature    5146..5165 
                     /label="Restriction Enzyme"  
     misc_feature    5950..6622 
                     /label="ColE1"  
     misc_feature    7052..7846 
                     /label="Kan/Neo"  
     misc_feature    7881..8183 
                     /label="SV40 ori"  
     misc_feature    8269..8746 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4950..4956 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4950 
                     /label="PARD3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGACC GTGTGCTTCG GACGGACCCG GGTGGTCGTG CCGTGCGGGG 
     1081 ACGGCCACAT GAAAGTTTTC AGCCTCATCC AGCAGGCGGT GACCCGCTAC CGGAAGGCCA 
     1141 TCGCCAAGGA TCCAAACTAC TGGATACAGG TGCATCGCTT GGAACATGGA GATGGAGGAA 
     1201 TACTAGACCT TGATGACATT CTTTGTGATG TAGCAGACGA TAAAGACAGA CTGGTAGCAG 
     1261 TGTTTGATGA GCAGGATCCA CATCACGGAG GTGATGGCAC CAGTGCCAGT TCCACGGGTA 
     1321 CCCAGAGCCC AGAGATATTT GGTAGTGAGC TTGGCACCAA CAATGTCTCA GCCTTTCAGC 
     1381 CTTACCAAGC AACAAGTGAA ATTGAGGTCA CACCTTCAGT CCTTCGAGCA AATATGCCTC 
     1441 TTCATGTTCG ACGCAGTAGT GACCCAGCTC TAATTGGCCT CTCCACTTCT GTCAGTGATA 
     1501 GTAATTTTTC CTCTGAAGAG CCTTCAAGGA AAAATCCCAC ACGCTGGTCA ACAACAGCTG 
     1561 GCTTCCTCAA GCAGAACACT GCTGGGAGTC CTAAAACCTG CGACAGGAAG AAAGATGAAA 
     1621 ACTACAGAAG CCTCCCGCGG GATACTAGTA ACTGGTCTAA CCAATTTCAG AGAGACAATG 
     1681 CTCGCTCGTC TCTGAGTGCC AGTCACCCAA TGGTGGGCAA GTGGCTGGAG AAACAAGAAC 
     1741 AGGATGAGGA TGGGACAGAA GAGGATAACA GTCGTGTTGA ACCTGTTGGA CATGCTGACA 
     1801 CGGGTTTGGA GCATATACCC AACTTTTCTC TGGATGATAT GGTAAAGCTC GTAGAAGTCC 
     1861 CCAACGATGG AGGGCCTCTG GGAATCCATG TAGTGCCTTT CAGTGCTCGA GGCGGCAGAA 
     1921 CCCTGGGGTT ATTAGTAAAA CGATTGGAGA AAGGTGGTAA AGCTGAACAT GAAAATCTTT 
     1981 TTCGTGAGAA TGATTGCATT GTCAGGATTA ATGATGGCGA CCTTCGAAAT AGAAGATTTG 
     2041 AACAAGCACA ACATATGTTT CGCCAAGCCA TGCGTACACC CATCATTTGG TTCCATGTGG 
     2101 TTCCTGCAGC AAATAAAGAG CAGTATGAAC AACTATCCCA AAGTGAGAAG AACAATTACT 
     2161 ATTCAAGCCG TTTTAGCCCT GACAGCCAGT ATATTGACAA CAGGAGTGTG AACAGTGCAG 
     2221 GGCTTCACAC GGTGCAGAGA GCACCCCGAC TGAACCACCC GCCTGAGCAG ATAGACTCTC 
     2281 ACTCAAGACT ACCTCATAGC GCACACCCCT CGGGAAAACC ACCATCCGCT CCAGCCTCGG 
     2341 CACCTCAGAA TGTATTTAGT ACGACTGTAA GCAGTGGTTA TAACACCAAA AAAATAGGCA 
     2401 AGAGGCTTAA TATCCAGCTT AAGAAAGGTA CAGAAGGTTT GGGATTCAGC ATCACTTCCA 
     2461 GAGATGTAAC AATAGGTGGC TCAGCTCCAA TCTATGTGAA AAACATTCTC CCCCGGGGGG 
     2521 CGGCCATTCA GGATGGCCGA CTTAAGGCAG GAGACAGACT TATAGAGGTA AATGGAGTAG 
     2581 ATTTAGTGGG CAAATCCCAA GAGGAAGTTG TTTCGCTGTT GAGAAGCACC AAGATGGAAG 
     2641 GAACTGTGAG CCTTCTGGTC TTTCGCCAGG AAGACGCCTT CCACCCAAGG GAACTGAAAG 
     2701 CAGAAGATGA GGATATTGTT CTTACACCTG ATGGCACCAG GGAATTTCTG ACATTTGAAG 
     2761 TCCCACTTAA TGATTCAGGA TCTGCAGGCC TTGGTGTCAG TGTCAAAGGT AACCGGTCAA 
     2821 AAGAGAACCA CGCAGATTTG GGAATCTTTG TCAAGTCCAT TATTAATGGA GGAGCAGCAT 
     2881 CTAAAGATGG AAGGCTTCGG GTGAATGATC AACTGATAGC AGTAAATGGA GAATCCCTGT 
     2941 TGGGCAAGAC AAACCAAGAT GCCATGGAAA CCCTAAGAAG GTCTATGTCT ACTGAAGGCA 
     3001 ATAAACGAGG AATGATCCAG CTTATTGTTG CAAGGAGAAT AAGCAAGTGC AATGAGCTGA 
     3061 AGTCACCTGG GAGCCCCCCT GGACCTGAGC TGCCCATTGA AACAGCGTTG GATGATAGAG 
     3121 AACGAAGAAT TTCCCATTCC CTCTACAGTG GGATTGAGGG GCTTGATGAA TCGCCCAGCA 
     3181 GAAATGCTGC CCTCAGTAGG ATAATGGGTA AATACCAGCT GTCCCCTACA GTGAATATGC 
     3241 CCCAAGATGA CACTGTCATT ATAGAAGATG ACAGGTTGCC AGTGCTTCCT CCACATCTCT 
     3301 CTGACCAGTC CTCTTCCAGC TCCCATGATG ATGTGGGGTT TGTGACGGCA GATGCTGGTA 
     3361 CTTGGGCCAA GGCTGCAATC AGTGATTCAG CCGACTGCTC TTTGAGTCCA GATGTTGATC 
     3421 CAGTTCTTGC TTTTCAACGA GAAGGATTTG GACGTCAGAT AGCTGACGAG ACTAAACTCA 
     3481 ATACAGTGGA TGACCAGAAA GCAGGTTCTC CCAGCAGAGA TGTGGGTCCT TCCCTGGGTC 
     3541 TGAAGAAGTC AAGCTCGTTG GAGAGTCTGC AGACCGCAGT TGCCGAGGTG ACTTTGAATG 
     3601 GGGATATTCC TTTCCATCGT CCACGGCCGC GGATAATCAG AGGCAGGGGA TGCAATGAGA 
     3661 GCTTCAGAGC TGCCATCGAC AAATCTTATG ATAAACCCGC GGTAGATGAT GATGATGAAG 
     3721 GCATGGAGAC CTTGGAAGAA GACACAGAAG AAAGTTCAAG ATCAGGGAGA GAGTCTGTAT 
     3781 CCACAGCCAG TGATCAGCCT TCCCACTCTC TGGAGAGACA AATGAATGGA AACCAAGAGA 
     3841 AAGGTGATAA GACTGATAGA AAAAAGGATA AAACTGGAAA AGAAAAGAAG AAAGATAGAG 
     3901 ATAAGGAGAA GGATAAAATG AAAGCCAAGA AGGGAATGCT GAAGGGCTTG GGAGACATGT 
     3961 TCAGGTTTGG CAAACATCGA AAAGATGACA AGATTGAGAA AACGGGTAAA ATAAAAATAC 
     4021 AGGAATCCTT TACATCAGAA GAGGAGAGGA TACGAATGAA GCAGGAGCAG GAGAGGATTC 
     4081 AAGCCAAAAC TCGAGAATTT AGGGAACGAC AAGCTCGAGA GCGTGACTAT GCTGAAATTC 
     4141 AAGATTTTCA TCGGACATTT GGCTGTGATG ATGAGTTAAT GTATGGGGGA GTTTCTTCTT 
     4201 ATGAAGGTTC CATGGCTCTC AACGCTAGAC CTCAGAGCCC ACGAGAAGGG CATATGATGG 
     4261 ATGCTTTGTA TGCCCAAGTC AAGAAGCCGC GGAATTCCAA ACCCTCACCT GTAGACAGTA 
     4321 ACAGATCAAC TCCTAGCAAT CATGATCGGA TACAGCGTCT GAGGCAAGAA TTTCAGCAAG 
     4381 CAAAGCAAGA TGAAGATGTA GAAGATCGTC GGCGGACCTA TAGTTTTGAG CAACCCTGGC 
     4441 CGAACGCACG GCCGGCGACG CAGAGCGGGC GACACTCGGT GTCCGTGGAG GTGCAGATGC 
     4501 AGCGGCAGCG GCAGGAGGAG CGCGAGAGCT CCCAGCAGGC CCAGCGCCAG TACAGCTCTC 
     4561 TGCCTCGGCA AAGCAGGAAA AATGCCAGCT CGGTCTCCCA GGACTCTTGG GAGCAGAACT 
     4621 ACTCCCCTGG GGAAGGCTTC CAGAGTGCCA AAGAGAACCC CAGGTACTCC AGCTACCAAG 
     4681 GCTCCAGGAA CGGCTACCTG GGAGGACATG GCTTCAACGC CAGGGTCATG CTGGAAACTC 
     4741 AGGAGCTCCT TCGCCAGGAA CAGAGGCGGA AGGAGCAGCA GATGAAGAAG CAGCCTCCTT 
     4801 CCGAGGGGCC CAGCAACTAT GACTCGTATA AGAAAGTCCA GGACCCCAGT TACGCCCCTC 
     4861 CCAAGGGGCC CTTCCGGCAA GATGTGCCCC CCTCCCCTTC TCAGGTTGCG AGGCTGAACA 
     4921 GACTTCAGAC TCCTGAGAAA GGGAGGCCCT TCTATTCCAC GCGTACGCGG CCGCTCGAGC 
     4981 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5041 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5101 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5161 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5221 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5281 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5341 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5401 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5461 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5521 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5581 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5641 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5701 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5761 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5821 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5881 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5941 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6001 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6061 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6121 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6181 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6241 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6301 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6361 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6421 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6481 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6541 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6601 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6661 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6721 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6781 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6841 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6901 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6961 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7021 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7081 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7141 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7201 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7261 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7321 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7381 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7441 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7501 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7561 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7621 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7681 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7741 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7801 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7861 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7921 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7981 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8041 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8101 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8161 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8221 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8281 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8341 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8401 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8461 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8521 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8581 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8641 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8701 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8761 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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