LOCUS       RC230692                8709 bp    DNA     circular  SYN 
DEFINITION  AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 4
ACCESSION   NM_001169124
VERSION     NM_001169124.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4866..4957 
                     /label="Myc-DDK"  
     misc_feature    4987..5572 
                     /label="PolyA signal"  
     misc_feature    5044..5063 
                     /label="Restriction Enzyme"  
     misc_feature    5848..6520 
                     /label="ColE1"  
     misc_feature    6950..7744 
                     /label="Kan/Neo"  
     misc_feature    7779..8081 
                     /label="SV40 ori"  
     misc_feature    8167..8644 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4848..4854 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4848 
                     /label="AFF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCTATTC GACTTTTTCA GAGACTGGGA CTTGGAGCAG CAGTGTCACT 
     1081 ATGAACAAGA CCGTAGTGCA CTTAAAAAAA GGGAATGGGA GCGGAGGAAT CAAGAAGTCC 
     1141 AGCAAGAAGA CGATCTCTTT TCTTCAGGCT TTGATCTTTT TGGGGAGCCA TACAAGGTAG 
     1201 CTGAATATAC AAACAAAGGT GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT 
     1261 ATGATGAAAT GAAGAATTTG CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC 
     1321 CAAAGAATTC TGTGCCCCAG AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC 
     1381 AAAAGAACAG AATAATTCCA CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC 
     1441 CACCTTCTGT TGTGATACTG AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG 
     1501 AGTGGTCACG TGATAGTCAT AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC 
     1561 CAAACAAGAT GCAGACTTTG ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG 
     1621 TCTACCCAGC TGAACAGCCC CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG 
     1681 AAGACAGTAA CCCTAATTCT AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT 
     1741 CACCGGAAGA ATCTGAATTC GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT 
     1801 TATTAGCTCC TAGCAGTGGC CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA 
     1861 CAAGCATGGG GCAGCAAAAG CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG 
     1921 CACCGGACAT CTCACCAACA CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC 
     1981 TGTCATTTGG AACACTCTTG GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC 
     2041 CAAAGTTCAC CATCCTCCAA ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG 
     2101 AAGAAATCTT GCGGGAATCG CAGCATCTGA CCCCAGGATT CACCTTACAA AAGTGGAATG 
     2161 ACCCAACCAC CAGAGCTTCT ACAAAGATGC TTGAGGATGA CCTGAAGCTG AGCAGTGATG 
     2221 AAGATGACCT TGAGCCTGTG AAGACCTTGA CCACTCAGTG CACTGCCACT GAGCTCTACC 
     2281 AGGCTGTTGA AAAGGCAAAA CCTAGGAATA ATCCTGTGAA CCCACCCTTG GCCACTCCCC 
     2341 AGCCCCCACC TGCAGTGCAA GCCAGCGGGG GTTCTGGCAG CTCCAGCGAA TCGGAGAGCA 
     2401 GCTCTGAGTC GGATTCAGAC ACTGAAAGTA GCACCACTGA CAGCGAATCT AATGAGGCAC 
     2461 CTCGTGTGGC AACTCCAGAG CCTGAGCCAC CCTCAACCAA CAAGTGGCAA CTGGATAAAT 
     2521 GGCTTAACAA AGTGACATCC CAGAACAAGT CTTTTATTTG TGGCCAAAAT GAAACACCCA 
     2581 TGGAGACTAT TTCTCTGCCT CCTCCAATCA TCCAACCAAT GGAAGTCCAG ATGAAAGTGA 
     2641 AGACGAATGC CAGTCAGGTC CCAGCTGAAC CCAAAGAAAG GCCTCTCCTC AGTCTCATTA 
     2701 GGGAGAAAGC CCGTCCACGG CCCACTCAGA AAATTCCAGA AACAAAGGCT TTGAAGCATA 
     2761 AGTTGTCAAC AACTAGTGAG ACAGTGTCTC AAAGGACAAT TGGGAAAAAA CAGCCCAAAA 
     2821 AAGTTGAGAA GAACACCAGC ACTGACGAGT TTACCTGGCC CAAACCAAAT ATTACCAGCA 
     2881 GCACTCCCAA AGAAAAAGAA AGTGTGGAGC TTCATGACCC ACCAAGAGGC CGCAACAAAG 
     2941 CCACTGCCCA CAAACCAGCC CCTAGGAAAG AACCAAGACC TAACATCCCT TTGGCTCCCG 
     3001 AGAAGAAGAA GTACAGAGGG CCTGGCAAGA TTGTGCCAAA GTCTCGGGAA TTCATTGAAA 
     3061 CAGATTCATC TACATCTGAC TCCAACACAG ATCAGGAAGA GACCCTGCAA ATCAAAGTCC 
     3121 TGCCTCCGTG CATTATTTCT GGAGGTAATA CTGCCAAATC CAAGGAAATC TGTGGTGCCA 
     3181 GCCTGACCCT CAGCACCTTA ATGAGTAGCA GTGGCAGCAA CAACAACTTA TCCATCAGTA 
     3241 ATGAAGAGCC AACATTTTCA CCTATTCCTG TCATGCAAAC TGAAATCCTG TCCCCTCTGC 
     3301 GAGATCATGA GAACCTGAAA AACCTCTGGG TGAAGATTGA CCTTGACTTA CTCTCTAGAG 
     3361 TACCTGGCCA CAGCTCACTC CATGCAGCAC CTGCCAAGCC AGACCACAAG GAGACTGCCA 
     3421 CAAAACCCAA GCGTCAGACA GCTGTCACAG CTGTGGAGAA ACCAGCCCCT AAGGGCAAAC 
     3481 GTAAGCACAA GCCAATAGAA GTTGCAGAGA AGATCCCTGA GAAGAAGCAG CGCCTGGAGG 
     3541 AGGCCACAAC TATCTGCTTG CTCCCTCCTT GCATCTCACC AGCCCCACCC CACAAGCCTC 
     3601 CCAACACTAG AGAAAATAAT TCATCCAGGA GAGCAAATAG AAGAAAGGAA GAAAAACTAT 
     3661 TTCCTCCTCC ACTTTCCCCA CTGCCAGAGG ACCCTCCACG CCGCAGAAAT GTCAGTGGCA 
     3721 ATAATGGTCC CTTTGGTCAA GACAAAAACA TCGCCATGAC TGGACAAATC ACATCTACCA 
     3781 AACCTAAGAG AACTGAAGGC AAATTCTGTG CTACTTTCAA AGGGATATCG GTAAATGAGG 
     3841 GAGACACTCC AAAAAAGGCA TCCTCTGCCA CCATCACTGT CACCAATACT GCTATTGCCA 
     3901 CTGCTACTGT CACTGCTACT GCCATTGTCA CCACCACTGT CACAGCTACT GCCACCGCCA 
     3961 CGGCCACCAC CACAACTACT ACCACTACCA TTTCCACCAT CACCTCTACC ATCACTACTG 
     4021 GCCTCATGGA TAGCAGTCAC CTGGAGATGA CGTCCTGGGC GGCTCTGCCC CTTCTATCCA 
     4081 GCAGCAGCAC TAATGTCCGG AGACCCAAGC TCACTTTTGA TGACTCGGTT CACAATGCTG 
     4141 ATTATTACAT GCAAGAAGCT AAGAAGCTGA AGCACAAAGC TGATGCACTG TTCGAGAAAT 
     4201 TTGGCAAAGC TGTGAATTAT GCTGATGCCG CCCTCTCCTT CACTGAATGT GGCAATGCCA 
     4261 TGGAACGCGA CCCTCTGGAA GCAAAGTCCC CATACACCAT GTACTCTGAG ACTGTGGAGC 
     4321 TCCTCAGGTA TGCAATGAGG CTGAAGAACT TTGCAAGTCC CTTGGCTTCG GATGGGGACA 
     4381 AAAAGCTAGC AGTACTATGC TACCGATGTT TATCACTCCT CTATTTGAGA ATGTTTAAGC 
     4441 TGAAGAAGGA CCATGCTATG AAGTACTCCA GATCACTGAT GGAATATTTT AAGCAAAATG 
     4501 CTTCAAAAGT CGCACAGATA CCCTCTCCAT GGGTAAGCAA TGGAAAGAAC ACTCCATCCC 
     4561 CAGTGTCTCT CAACAACGTC TCCCCCATCA ACGCAATGGG GAACTGTAAC AATGGCCCAG 
     4621 TCACCATTCC CCAGCGCATT CACCACATGG CTGCCAGCCA CGTCAACATC ACTAGCAATG 
     4681 TGTTACGGGG CTATGAACAC TGGGATATGG CCGACAAACT GACAAGAGAA AACAAAGAAT 
     4741 TCTTTGGTGA TCTGGACACG CTGATGGGGC CTCTGACCCA GCACAGCAGC ATGACCAATC 
     4801 TTGTCCGCTA CGTTCGCCAA GGACTGTGTT GGCTGCGCAT CGATGCCCAC TTGTTGACGC 
     4861 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4921 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4981 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5041 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5101 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5161 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5221 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5281 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5341 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5401 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5461 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5521 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5581 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5641 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5701 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5761 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5821 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5881 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5941 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6001 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6061 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6121 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6181 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6241 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6301 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6361 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6421 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6481 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6541 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6601 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6661 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6721 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6781 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6841 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6901 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6961 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7021 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7081 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7141 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7201 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7261 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7321 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7381 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7441 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7501 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7561 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7621 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7681 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7741 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7801 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7861 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7921 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7981 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8041 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8101 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8161 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8221 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8281 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8341 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8401 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8461 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8521 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8581 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8641 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8701 GCGAATTTT
//