LOCUS       RC230688                8697 bp    DNA     circular  SYN 
DEFINITION  AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 5
ACCESSION   NM_001169125
VERSION     NM_001169125.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4854..4945 
                     /label="Myc-DDK"  
     misc_feature    4975..5560 
                     /label="PolyA signal"  
     misc_feature    5032..5051 
                     /label="Restriction Enzyme"  
     misc_feature    5836..6508 
                     /label="ColE1"  
     misc_feature    6938..7732 
                     /label="Kan/Neo"  
     misc_feature    7767..8069 
                     /label="SV40 ori"  
     misc_feature    8155..8632 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4836..4842 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4836 
                     /label="AFF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCTATTC GACTTTTTCA GAGACTGGGA CTTGGAGCAG CAGTGTCACT 
     1081 ATGAACAAGA CCGTAGTGCA CTTAAAAAAA GGGAATGGGA GCGGAGGAAT CAAGAAGTCC 
     1141 AGCAAGAAGA CGATCTCTTT TCTTCAGGCT TTGATCTTTT TGGGGAGCCA TACAAGACAA 
     1201 ACAAAGGTGA TGCACTTGCC AACCGAGTCC AGAACACGCT TGGAAACTAT GATGAAATGA 
     1261 AGAATTTGCT AACTAACCAT TCTAATCAGA ATCACCTAGT GGGAATTCCA AAGAATTCTG 
     1321 TGCCCCAGAA TCCCAACAAC AAAAATGAAC CAAGCTTTTT TCCAGAACAA AAGAACAGAA 
     1381 TAATTCCACC TCACCAGGAT AATACCCATC CTTCAGCACC AATGCCTCCA CCTTCTGTTG 
     1441 TGATACTGAA TTCAACTCTA ATACACAGCA ACAGAAAATC AAAACCTGAG TGGTCACGTG 
     1501 ATAGTCATAA CCCTAGCACT GTACTGGCAA GCCAGGCCAG TGGTCAGCCA AACAAGATGC 
     1561 AGACTTTGAC ACAGGACCAG TCTCAAGCCA AACTGGAAGA CTTCTTTGTC TACCCAGCTG 
     1621 AACAGCCCCA GATTGGAGAA GTTGAAGAGT CAAACCCATC TGCAAAGGAA GACAGTAACC 
     1681 CTAATTCTAG TGGAGAAGAT GCTTTCAAAG AAATCTTTCA ATCCAATTCA CCGGAAGAAT 
     1741 CTGAATTCGC CGTGCAAGCG CCTGGGTCTC CCCTAGTGGC TTCCTCTTTA TTAGCTCCTA 
     1801 GCAGTGGCCT TTCAGTTCAA AACTTCCCAC CAGGGCTTTA CTGCAAAACA AGCATGGGGC 
     1861 AGCAAAAGCC AACTGCATAC GTCAGACCCA TGGATGGCCA GGACCAGGCA CCGGACATCT 
     1921 CACCAACACT GAAACCTTCA ATTGAATTTG AGAACAGCTT TGGGAATCTG TCATTTGGAA 
     1981 CACTCTTGGA TGGAAAACCC AGTGCAGCCA GTTCAAAGAC TAAACTGCCA AAGTTCACCA 
     2041 TCCTCCAAAC AAGTGAAGTA AGCCTTCCCA GTGATCCAAG CTGTGTTGAA GAAATCTTGC 
     2101 GGGAATCGCA GCATCTGACC CCAGGATTCA CCTTACAAAA GTGGAATGAC CCAACCACCA 
     2161 GAGCTTCTAC AAAGATGCTT GAGGATGACC TGAAGCTGAG CAGTGATGAA GATGACCTTG 
     2221 AGCCTGTGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG GCTGTTGAAA 
     2281 AGGCAAAACC TAGGAATAAT CCTGTGAACC CACCCTTGGC CACTCCCCAG CCCCCACCTG 
     2341 CAGTGCAAGC CAGCGGGGGT TCTGGCAGCT CCAGCGAATC GGAGAGCAGC TCTGAGTCGG 
     2401 ATTCAGACAC TGAAAGTAGC ACCACTGACA GCGAATCTAA TGAGGCACCT CGTGTGGCAA 
     2461 CTCCAGAGCC TGAGCCACCC TCAACCAACA AGTGGCAACT GGATAAATGG CTTAACAAAG 
     2521 TGACATCCCA GAACAAGTCT TTTATTTGTG GCCAAAATGA AACACCCATG GAGACTATTT 
     2581 CTCTGCCTCC TCCAATCATC CAACCAATGG AAGTCCAGAT GAAAGTGAAG ACGAATGCCA 
     2641 GTCAGGTCCC AGCTGAACCC AAAGAAAGGC CTCTCCTCAG TCTCATTAGG GAGAAAGCCC 
     2701 GTCCACGGCC CACTCAGAAA ATTCCAGAAA CAAAGGCTTT GAAGCATAAG TTGTCAACAA 
     2761 CTAGTGAGAC AGTGTCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAAA GTTGAGAAGA 
     2821 ACACCAGCAC TGACGAGTTT ACCTGGCCCA AACCAAATAT TACCAGCAGC ACTCCCAAAG 
     2881 AAAAAGAAAG TGTGGAGCTT CATGACCCAC CAAGAGGCCG CAACAAAGCC ACTGCCCACA 
     2941 AACCAGCCCC TAGGAAAGAA CCAAGACCTA ACATCCCTTT GGCTCCCGAG AAGAAGAAGT 
     3001 ACAGAGGGCC TGGCAAGATT GTGCCAAAGT CTCGGGAATT CATTGAAACA GATTCATCTA 
     3061 CATCTGACTC CAACACAGAT CAGGAAGAGA CCCTGCAAAT CAAAGTCCTG CCTCCGTGCA 
     3121 TTATTTCTGG AGGTAATACT GCCAAATCCA AGGAAATCTG TGGTGCCAGC CTGACCCTCA 
     3181 GCACCTTAAT GAGTAGCAGT GGCAGCAACA ACAACTTATC CATCAGTAAT GAAGAGCCAA 
     3241 CATTTTCACC TATTCCTGTC ATGCAAACTG AAATCCTGTC CCCTCTGCGA GATCATGAGA 
     3301 ACCTGAAAAA CCTCTGGGTG AAGATTGACC TTGACTTACT CTCTAGAGTA CCTGGCCACA 
     3361 GCTCACTCCA TGCAGCACCT GCCAAGCCAG ACCACAAGGA GACTGCCACA AAACCCAAGC 
     3421 GTCAGACAGC TGTCACAGCT GTGGAGAAAC CAGCCCCTAA GGGCAAACGT AAGCACAAGC 
     3481 CAATAGAAGT TGCAGAGAAG ATCCCTGAGA AGAAGCAGCG CCTGGAGGAG GCCACAACTA 
     3541 TCTGCTTGCT CCCTCCTTGC ATCTCACCAG CCCCACCCCA CAAGCCTCCC AACACTAGAG 
     3601 AAAATAATTC ATCCAGGAGA GCAAATAGAA GAAAGGAAGA AAAACTATTT CCTCCTCCAC 
     3661 TTTCCCCACT GCCAGAGGAC CCTCCACGCC GCAGAAATGT CAGTGGCAAT AATGGTCCCT 
     3721 TTGGTCAAGA CAAAAACATC GCCATGACTG GACAAATCAC ATCTACCAAA CCTAAGAGAA 
     3781 CTGAAGGCAA ATTCTGTGCT ACTTTCAAAG GGATATCGGT AAATGAGGGA GACACTCCAA 
     3841 AAAAGGCATC CTCTGCCACC ATCACTGTCA CCAATACTGC TATTGCCACT GCTACTGTCA 
     3901 CTGCTACTGC CATTGTCACC ACCACTGTCA CAGCTACTGC CACCGCCACG GCCACCACCA 
     3961 CAACTACTAC CACTACCATT TCCACCATCA CCTCTACCAT CACTACTGGC CTCATGGATA 
     4021 GCAGTCACCT GGAGATGACG TCCTGGGCGG CTCTGCCCCT TCTATCCAGC AGCAGCACTA 
     4081 ATGTCCGGAG ACCCAAGCTC ACTTTTGATG ACTCGGTTCA CAATGCTGAT TATTACATGC 
     4141 AAGAAGCTAA GAAGCTGAAG CACAAAGCTG ATGCACTGTT CGAGAAATTT GGCAAAGCTG 
     4201 TGAATTATGC TGATGCCGCC CTCTCCTTCA CTGAATGTGG CAATGCCATG GAACGCGACC 
     4261 CTCTGGAAGC AAAGTCCCCA TACACCATGT ACTCTGAGAC TGTGGAGCTC CTCAGGTATG 
     4321 CAATGAGGCT GAAGAACTTT GCAAGTCCCT TGGCTTCGGA TGGGGACAAA AAGCTAGCAG 
     4381 TACTATGCTA CCGATGTTTA TCACTCCTCT ATTTGAGAAT GTTTAAGCTG AAGAAGGACC 
     4441 ATGCTATGAA GTACTCCAGA TCACTGATGG AATATTTTAA GCAAAATGCT TCAAAAGTCG 
     4501 CACAGATACC CTCTCCATGG GTAAGCAATG GAAAGAACAC TCCATCCCCA GTGTCTCTCA 
     4561 ACAACGTCTC CCCCATCAAC GCAATGGGGA ACTGTAACAA TGGCCCAGTC ACCATTCCCC 
     4621 AGCGCATTCA CCACATGGCT GCCAGCCACG TCAACATCAC TAGCAATGTG TTACGGGGCT 
     4681 ATGAACACTG GGATATGGCC GACAAACTGA CAAGAGAAAA CAAAGAATTC TTTGGTGATC 
     4741 TGGACACGCT GATGGGGCCT CTGACCCAGC ACAGCAGCAT GACCAATCTT GTCCGCTACG 
     4801 TTCGCCAAGG ACTGTGTTGG CTGCGCATCG ATGCCCACTT GTTGACGCGT ACGCGGCCGC 
     4861 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4921 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4981 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5041 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5101 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5161 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5221 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5281 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5341 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5401 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5461 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5521 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5581 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5641 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5701 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5761 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5821 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5881 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5941 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6001 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6061 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6121 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6181 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6241 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6301 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6361 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6421 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6481 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6541 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6601 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6661 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6721 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6781 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6841 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6901 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6961 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7021 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7081 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7141 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7201 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7261 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7321 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7381 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7441 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7501 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7561 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7621 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7681 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7741 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7801 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7861 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7921 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8041 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8101 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8161 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8221 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8281 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8341 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8401 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8461 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8521 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8581 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8641 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//