LOCUS       RC230686                8679 bp    DNA     circular  SYN 
DEFINITION  PARD3 (Myc-DDK-tagged)-Human par-3 partitioning defective 3 homolog (C. elegans) (PARD3), transcript variant 7
ACCESSION   NM_001184790
VERSION     NM_001184790.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4836..4927 
                     /label="Myc-DDK"  
     misc_feature    4957..5542 
                     /label="PolyA signal"  
     misc_feature    5014..5033 
                     /label="Restriction Enzyme"  
     misc_feature    5818..6490 
                     /label="ColE1"  
     misc_feature    6920..7714 
                     /label="Kan/Neo"  
     misc_feature    7749..8051 
                     /label="SV40 ori"  
     misc_feature    8137..8614 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4818..4824 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4818 
                     /label="PARD3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGACC GTGTGCTTCG GACGGACCCG GGTGGTCGTG CCGTGCGGGG 
     1081 ACGGCCACAT GAAAGTTTTC AGCCTCATCC AGCAGGCGGT GACCCGCTAC CGGAAGGCCA 
     1141 TCGCCAAGGA TCCAAACTAC TGGATACAGG TGCATCGCTT GGAACATGGA GATGGAGGAA 
     1201 TACTAGACCT TGATGACATT CTTTGTGATG TAGCAGACGA TAAAGACAGA CTGGTAGCAG 
     1261 TGTTTGATGA GCAGGATCCA CATCACGGAG GTGATGGCAC CAGTGCCAGT TCCACGGGTA 
     1321 CCCAGAGCCC AGAGATATTT GGTAGTGAGC TTGGCACCAA CAATGTCTCA GCCTTTCAGC 
     1381 CTTACCAAGC AACAAGTGAA ATTGAGGTCA CACCTTCAGT CCTTCGAGCA AATATGCCTC 
     1441 TTCATGTTCG ACGCAGTAGT GACCCAGCTC TAATTGGCCT CTCCACTTCT GTCAGTGATA 
     1501 GTAATTTTTC CTCTGAAGAG CCTTCAAGGA AAAATCCCAC ACGCTGGTCA ACAACAGCTG 
     1561 GCTTCCTCAA GCAGAACACT GCTGGGAGTC CTAAAACCTG CGACAGGAAG GATGAGGATG 
     1621 GGACAGAAGA GGATAACAGT CGTGTTGAAC CTGTTGGACA TGCTGACACG GGTTTGGAGC 
     1681 ATATACCCAA CTTTTCTCTG GATGATATGG TAAAGCTCGT AGAAGTCCCC AACGATGGAG 
     1741 GGCCTCTGGG AATCCATGTA GTGCCTTTCA GTGCTCGAGG CGGCAGAACC CTGGGGTTAT 
     1801 TAGTAAAACG ATTGGAGAAA GGTGGTAAAG CTGAACATGA AAATCTTTTT CGTGAGAATG 
     1861 ATTGCATTGT CAGGATTAAT GATGGCGACC TTCGAAATAG AAGATTTGAA CAAGCACAAC 
     1921 ATATGTTTCG CCAAGCCATG CGTACACCCA TCATTTGGTT CCATGTGGTT CCTGCAGCAA 
     1981 ATAAAGAGCA GTATGAACAA CTATCCCAAA GTGAGAAGAA CAATTACTAT TCAAGCCGTT 
     2041 TTAGCCCTGA CAGCCAGTAT ATTGACAACA GGAGTGTGAA CAGTGCAGGG CTTCACACGG 
     2101 TGCAGAGAGC ACCCCGACTG AACCACCCGC CTGAGCAGAT AGACTCTCAC TCAAGACTAC 
     2161 CTCATAGCGC ACACCCCTCG GGAAAACCAC CATCCGCTCC AGCCTCGGCA CCTCAGAATG 
     2221 TATTTAGTAC GACTGTAAGC AGTGGTTATA ACACCAAAAA AATAGGCAAG AGGCTTAATA 
     2281 TCCAGCTTAA GAAAGGTACA GAAGGTTTGG GATTCAGCAT CACTTCCAGA GATGTAACAA 
     2341 TAGGTGGCTC AGCTCCAATC TATGTGAAAA ACATTCTCCC CCGGGGGGCG GCCATTCAGG 
     2401 ATGGCCGACT TAAGGCAGGA GACAGACTTA TAGAGGTAAA TGGAGTAGAT TTAGTGGGCA 
     2461 AATCCCAAGA GGAAGTTGTT TCGCTGTTGA GAAGCACCAA GATGGAAGGA ACTGTGAGCC 
     2521 TTCTGGTCTT TCGCCAGGAA GACGCCTTCC ACCCAAGGGA ACTGAAAGCA GAAGATGAGG 
     2581 ATATTGTTCT TACACCTGAT GGCACCAGGG AATTTCTGAC ATTTGAAGTC CCACTTAATG 
     2641 ATTCAGGATC TGCAGGCCTT GGTGTCAGTG TCAAAGGTAA CCGGTCAAAA GAGAACCACG 
     2701 CAGATTTGGG AATCTTTGTC AAGTCCATTA TTAATGGAGG AGCAGCATCT AAAGATGGAA 
     2761 GGCTTCGGGT GAATGATCAA CTGATAGCAG TAAATGGAGA ATCCCTGTTG GGCAAGACAA 
     2821 ACCAAGATGC CATGGAAACC CTAAGAAGGT CTATGTCTAC TGAAGGCAAT AAACGAGGAA 
     2881 TGATCCAGCT TATTGTTGCA AGGAGAATAA GCAAGTGCAA TGAGCTGAAG TCACCTGGGA 
     2941 GCCCCCCTGG ACCTGAGCTG CCCATTGAAA CAGCGTTGGA TGATAGAGAA CGAAGAATTT 
     3001 CCCATTCCCT CTACAGTGGG ATTGAGGGGC TTGATGAATC GCCCAGCAGA AATGCTGCCC 
     3061 TCAGTAGGAT AATGGGTAAA TACCAGCTGT CCCCTACAGT GAATATGCCC CAAGATGACA 
     3121 CTGTCATTAT AGAAGATGAC AGGTTGCCAG TGCTTCCTCC ACATCTCTCT GACCAGTCCT 
     3181 CTTCCAGCTC CCATGATGAT GTGGGGTTTG TGACGGCAGA TGCTGGTACT TGGGCCAAGG 
     3241 CTGCAATCAG TGATTCAGCC GACTGCTCTT TGAGTCCAGA TGTTGATCCA GTTCTTGCTT 
     3301 TTCAACGAGA AGGATTTGGA CGTCAGATAG CTGACGAGAC TAAACTCAAT ACAGTGGATG 
     3361 ACCAGAAAGC AGGTTCTCCC AGCAGAGATG TGGGTCCTTC CCTGGGTCTG AAGAAGTCAA 
     3421 GCTCGTTGGA GAGTCTGCAG ACCGCAGTTG CCGAGGTGAC TTTGAATGGG GATATTCCTT 
     3481 TCCATCGTCC ACGGCCGCGG ATAATCAGAG GCAGGGGATG CAATGAGAGC TTCAGAGCTG 
     3541 CCATCGACAA ATCTTATGAT AAACCCGCGG TAGATGATGA TGATGAAGGC ATGGAGACCT 
     3601 TGGAAGAAGA CACAGAAGAA AGTTCAAGAT CAGGGAGAGA GTCTGTATCC ACAGCCAGTG 
     3661 ATCAGCCTTC CCACTCTCTG GAGAGACAAA TGAATGGAAA CCAAGAGAAA GGTGATAAGA 
     3721 CTGATAGAAA AAAGGATAAA ACTGGAAAAG AAAAGAAGAA AGATAGAGAT AAGGAGAAGG 
     3781 ATAAAATGAA AGCCAAGAAG GGAATGCTGA AGGGCTTGGG AGACATGTTC AGGTTTGGCA 
     3841 AACATCGAAA AGATGACAAG ATTGAGAAAA CGGGTAAAAT AAAAATACAG GAATCCTTTA 
     3901 CATCAGAAGA GGAGAGGATA CGAATGAAGC AGGAGCAGGA GAGGATTCAA GCCAAAACTC 
     3961 GAGAATTTAG GGAACGACAA GCTCGAGAGC GTGACTATGC TGAAATTCAA GATTTTCATC 
     4021 GGACATTTGG CTGTGATGAT GAGTTAATGT ATGGGGGAGT TTCTTCTTAT GAAGGTTCCA 
     4081 TGGCTCTCAA CGCTAGACCT CAGAGCCCAC GAGAAGGGCA TATGATGGAT GCTTTGTATG 
     4141 CCCAAGTCAA GAAGCCGCGG AATTCCAAAC CCTCACCTGT AGACAGTAAC AGATCAACTC 
     4201 CTAGCAATCA TGATCGGATA CAGCGTCTGA GGCAAGAATT TCAGCAAGCA AAGCAAGATG 
     4261 AAGATGTAGA AGATCGTCGG CGGACCTATA GTTTTGAGCA ACCCTGGCCG AACGCACGGC 
     4321 CGGCGACGCA GAGCGGGCGA CACTCGGTGT CCGTGGAGGT GCAGATGCAG CGGCAGCGGC 
     4381 AGGAGGAGCG CGAGAGCTCC CAGCAGGCCC AGCGCCAGTA CAGCTCTCTG CCTCGGCAAA 
     4441 GCAGGAAAAA TGCCAGCTCG GTCTCCCAGG ACTCTTGGGA GCAGAACTAC TCCCCTGGGG 
     4501 AAGGCTTCCA GAGTGCCAAA GAGAACCCCA GGTACTCCAG CTACCAAGGC TCCAGGAACG 
     4561 GCTACCTGGG AGGACATGGC TTCAACGCCA GGGTCATGCT GGAAACTCAG GAGCTCCTTC 
     4621 GCCAGGAACA GAGGCGGAAG GAGCAGCAGA TGAAGAAGCA GCCTCCTTCC GAGGGGCCCA 
     4681 GCAACTATGA CTCGTATAAG AAAGTCCAGG ACCCCAGTTA CGCCCCTCCC AAGGGGCCCT 
     4741 TCCGGCAAGA TGTGCCCCCC TCCCCTTCTC AGGTTGCGAG GCTGAACAGA CTTCAGACTC 
     4801 CTGAGAAAGG GAGGCCCTTC TATTCCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4861 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4921 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4981 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5041 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5101 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5161 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5221 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5281 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5341 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5401 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5461 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5521 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5581 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5641 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5701 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5761 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5821 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5881 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5941 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6001 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6061 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6121 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6181 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6241 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6301 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6361 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6421 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6481 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6541 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6601 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6661 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6721 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6781 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6841 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6901 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6961 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7021 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7081 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7141 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7201 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7261 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7321 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7381 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7441 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7501 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7561 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7621 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7681 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7741 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7801 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7861 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7921 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7981 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8041 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8101 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8161 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8221 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8281 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8341 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8401 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8461 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8521 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8581 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8641 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//