LOCUS       RC230680                8613 bp    DNA     circular  SYN 
DEFINITION  PARD3 (Myc-DDK-tagged)-Human par-3 partitioning defective 3 homolog (C. elegans) (PARD3), transcript variant 8
ACCESSION   NM_001184791
VERSION     NM_001184791.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4770..4861 
                     /label="Myc-DDK"  
     misc_feature    4891..5476 
                     /label="PolyA signal"  
     misc_feature    4948..4967 
                     /label="Restriction Enzyme"  
     misc_feature    5752..6424 
                     /label="ColE1"  
     misc_feature    6854..7648 
                     /label="Kan/Neo"  
     misc_feature    7683..7985 
                     /label="SV40 ori"  
     misc_feature    8071..8548 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4752..4758 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4752 
                     /label="PARD3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGACC GTGTGCTTCG GACGGACCCG GGTGGTCGTG CCGTGCGGGG 
     1081 ACGGCCACAT GAAAGTTTTC AGCCTCATCC AGCAGGCGGT GACCCGCTAC CGGAAGGCCA 
     1141 TCGCCAAGGA TCCAAACTAC TGGATACAGG TGCATCGCTT GGAACATGGA GATGGAGGAA 
     1201 TACTAGACCT TGATGACATT CTTTGTGATG TAGCAGACGA TAAAGACAGA CTGGTAGCAG 
     1261 TGTTTGATGA GCAGGATCCA CATCACGGAG GTGATGGCAC CAGTGCCAGT TCCACGGGTA 
     1321 CCCAGAGCCC AGAGATATTT GGTAGTGAGC TTGGCACCAA CAATGTCTCA GCCTTTCAGC 
     1381 CTTACCAAGC AACAAGTGAA ATTGAGGTCA CACCTTCAGT CCTTCGAGCA AATATGCCTC 
     1441 TTCATGTTCG ACGCAGTAGT GACCCAGCTC TAATTGGCCT CTCCACTTCT GTCAGTGATA 
     1501 GTAATTTTTC CTCTGAAGAG CCTTCAAGGA AAAATCCCAC ACGCTGGTCA ACAACAGCTG 
     1561 GCTTCCTCAA GCAGAACACT GCTGGGAGTC CTAAAACCTG CGACAGGAAG GATGAGGATG 
     1621 GGACAGAAGA GGATAACAGT CGTGTTGAAC CTGTTGGACA TGCTGACACG GGTTTGGAGC 
     1681 ATATACCCAA CTTTTCTCTG GATGATATGG TAAAGCTCGT AGAAGTCCCC AACGATGGAG 
     1741 GGCCTCTGGG AATCCATGTA GTGCCTTTCA GTGCTCGAGG CGGCAGAACC CTGGGGTTAT 
     1801 TAGTAAAACG ATTGGAGAAA GGTGGTAAAG CTGAACATGA AAATCTTTTT CGTGAGAATG 
     1861 ATTGCATTGT CAGGATTAAT GATGGCGACC TTCGAAATAG AAGATTTGAA CAAGCACAAC 
     1921 ATATGTTTCG CCAAGCCATG CGTACACCCA TCATTTGGTT CCATGTGGTT CCTGCAGCAA 
     1981 ATAAAGAGCA GTATGAACAA CTATCCCAAA GTGAGAAGAA CAATTACTAT TCAAGCCGTT 
     2041 TTAGCCCTGA CAGCCAGTAT ATTGACAACA GGAGTGTGAA CAGTGCAGGG CTTCACACGG 
     2101 TGCAGAGAGC ACCCCGACTG AACCACCCGC CTGAGCAGAT AGACTCTCAC TCAAGACTAC 
     2161 CTCATAGCGC ACACCCCTCG GGAAAACCAC CATCCGCTCC AGCCTCGGCA CCTCAGAATG 
     2221 TATTTAGTAC GACTGTAAGC AGTGGTTATA ACACCAAAAA AATAGGCAAG AGGCTTAATA 
     2281 TCCAGCTTAA GAAAGGTACA GAAGGTTTGG GATTCAGCAT CACTTCCAGA GATGTAACAA 
     2341 TAGGTGGCTC AGCTCCAATC TATGTGAAAA ACATTCTCCC CCGGGGGGCG GCCATTCAGG 
     2401 ATGGCCGACT TAAGGCAGGA GACAGACTTA TAGAGGTAAA TGGAGTAGAT TTAGTGGGCA 
     2461 AATCCCAAGA GGAAGTTGTT TCGCTGTTGA GAAGCACCAA GATGGAAGGA ACTGTGAGCC 
     2521 TTCTGGTCTT TCGCCAGGAA GACGCCTTCC ACCCAAGGGA ACTGAAAGCA GAAGATGAGG 
     2581 ATATTGTTCT TACACCTGAT GGCACCAGGG AATTTCTGAC ATTTGAAGTC CCACTTAATG 
     2641 ATTCAGGATC TGCAGGCCTT GGTGTCAGTG TCAAAGGTAA CCGGTCAAAA GAGAACCACG 
     2701 CAGATTTGGG AATCTTTGTC AAGTCCATTA TTAATGGAGG AGCAGCATCT AAAGATGGAA 
     2761 GGCTTCGGGT GAATGATCAA CTGATAGCAG TAAATGGAGA ATCCCTGTTG GGCAAGACAA 
     2821 ACCAAGATGC CATGGAAACC CTAAGAAGGT CTATGTCTAC TGAAGGCAAT AAACGAGGAA 
     2881 TGATCCAGCT TATTGTTGCA AGGAGAATAA GCAAGTGCAA TGAGCTGAAG TCACCTGGGA 
     2941 GCCCCCCTGG ACCTGAGCTG CCCATTGAAA CAGCGTTGGA TGATAGAGAA CGAAGAATTT 
     3001 CCCATTCCCT CTACAGTGGG ATTGAGGGGC TTGATGAATC GCCCAGCAGA AATGCTGCCC 
     3061 TCAGTAGGAT AATGGGTAAA TACCAGCTGT CCCCTACAGT GAATATGCCC CAAGATGACA 
     3121 CTGTCATTAT AGAAGATGAC AGGTTGCCAG TGCTTCCTCC ACATCTCTCT GACCAGTCCT 
     3181 CTTCCAGCTC CCATGATGAT GTGGGGTTTG TGACGGCAGA TGCTGGTACT TGGGCCAAGG 
     3241 CTGCAATCAG TGATTCAGCC GACTGCTCTT TGAGTCCAGA TGTTGATCCA GTTCTTGCTT 
     3301 TTCAACGAGA AGGATTTGGA CGTCAGAGTA TGTCAGAAAA ACGCACAAAG CAATTTTCAG 
     3361 ATGCCAGTCA ATTGGATTTC GTTAAAACAC GAAAATCAAA AAGCATGGAT TTAGGTAGTT 
     3421 CTCCCAGCAG AGATGTGGGT CCTTCCCTGG GTCTGAAGAA GTCAAGCTCG TTGGAGAGTC 
     3481 TGCAGACCGC AGTTGCCGAG GTGACTTTGA ATGGGGATAT TCCTTTCCAT CGTCCACGGC 
     3541 CGCGGATAAT CAGAGGCAGG GGATGCAATG AGAGCTTCAG AGCTGCCATC GACAAATCTT 
     3601 ATGATAAACC CGCGGTAGAT GATGATGATG AAGGCATGGA GACCTTGGAA GAAGACACAG 
     3661 AAGAAAGTTC AAGATCAGGG AGAGAGTCTG TATCCACAGC CAGTGATCAG CCTTCCCACT 
     3721 CTCTGGAGAG ACAAATGAAT GGAAACCAAG AGAAAGGTGA TAAGACTGAT AGAAAAAAGG 
     3781 ATAAAACTGG AAAAGAAAAG AAGAAAGATA GAGATAAGGA GAAGGATAAA ATGAAAGCCA 
     3841 AGAAGGGAAT GCTGAAGGGC TTGGGAGACA TGTTCAGGAT TCAAGCCAAA ACTCGAGAAT 
     3901 TTAGGGAACG ACAAGCTCGA GAGCGTGACT ATGCTGAAAT TCAAGATTTT CATCGGACAT 
     3961 TTGGCTGTGA TGATGAGTTA ATGTATGGGG GAGTTTCTTC TTATGAAGGT TCCATGGCTC 
     4021 TCAACGCTAG ACCTCAGAGC CCACGAGAAG GGCATATGAT GGATGCTTTG TATGCCCAAG 
     4081 TCAAGAAGCC GCGGAATTCC AAACCCTCAC CTGTAGACAG TAACAGATCA ACTCCTAGCA 
     4141 ATCATGATCG GATACAGCGT CTGAGGCAAG AATTTCAGCA AGCAAAGCAA GATGAAGATG 
     4201 TAGAAGATCG TCGGCGGACC TATAGTTTTG AGCAACCCTG GCCGAACGCA CGGCCGGCGA 
     4261 CGCAGAGCGG GCGACACTCG GTGTCCGTGG AGGTGCAGAT GCAGCGGCAG CGGCAGGAGG 
     4321 AGCGCGAGAG CTCCCAGCAG GCCCAGCGCC AGTACAGCTC TCTGCCTCGG CAAAGCAGGA 
     4381 AAAATGCCAG CTCGGTCTCC CAGGACTCTT GGGAGCAGAA CTACTCCCCT GGGGAAGGCT 
     4441 TCCAGAGTGC CAAAGAGAAC CCCAGGTACT CCAGCTACCA AGGCTCCAGG AACGGCTACC 
     4501 TGGGAGGACA TGGCTTCAAC GCCAGGGTCA TGCTGGAAAC TCAGGAGCTC CTTCGCCAGG 
     4561 AACAGAGGCG GAAGGAGCAG CAGATGAAGA AGCAGCCTCC TTCCGAGGGG CCCAGCAACT 
     4621 ATGACTCGTA TAAGAAAGTC CAGGACCCCA GTTACGCCCC TCCCAAGGGG CCCTTCCGGC 
     4681 AAGATGTGCC CCCCTCCCCT TCTCAGGTTG CGAGGCTGAA CAGACTTCAG ACTCCTGAGA 
     4741 AAGGGAGGCC CTTCTATTCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4801 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4861 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4921 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4981 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5041 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5101 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5161 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5221 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5281 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5341 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5401 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5461 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5521 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5581 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5641 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5701 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5761 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5821 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5881 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5941 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6001 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6061 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6121 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6181 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6241 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6301 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6361 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6421 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6481 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6541 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6601 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6661 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6721 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6781 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6841 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6901 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6961 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7021 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7081 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7141 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7201 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7261 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7321 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7381 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7441 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7501 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7561 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7621 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7681 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7741 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7801 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7861 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8041 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8101 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8161 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8221 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8281 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8341 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8401 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8461 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8521 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8581 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//