LOCUS       RC230677                8544 bp    DNA     circular  SYN 
DEFINITION  TJP2 (Myc-DDK-tagged)-Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 3
ACCESSION   NM_001170416
VERSION     NM_001170416.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4701..4792 
                     /label="Myc-DDK"  
     misc_feature    4822..5407 
                     /label="PolyA signal"  
     misc_feature    4879..4898 
                     /label="Restriction Enzyme"  
     misc_feature    5683..6355 
                     /label="ColE1"  
     misc_feature    6785..7579 
                     /label="Kan/Neo"  
     misc_feature    7614..7916 
                     /label="SV40 ori"  
     misc_feature    8002..8479 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4683..4689 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4683 
                     /label="TJP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGACTGCT CAAGCCCTAC ATAGGATGTG GATCCAGGCT GTTAAAAAGT 
     1081 TGAGGAGATG GAAAGGCCGT GTGAGTCCCT CTGCAAGCTC TCCCCTTGTT TTCCCCAACC 
     1141 TTTCTTCATG GGAAGGGGAG GGAAGCAAAA CCATTCTCAC AGCCCCAGGC ATGGAAGAGC 
     1201 TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA TTTGGAATTG 
     1261 CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA ATTGTCATTT 
     1321 CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC AGAGTGGTCA 
     1381 TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG CAGCTCAGAA 
     1441 AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG GTGGCCGCAC 
     1501 TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG GACGAGTTTG 
     1561 ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC CATGGGGGGC 
     1621 GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG GCCCGGAGCC 
     1681 GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG GGCCTGGACC 
     1741 AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG GGCCTGGACC 
     1801 ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG AGCATTGACC 
     1861 AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG GCCTACAGCC 
     1921 CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA AGCCGCAGCC 
     1981 GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG GGGCCCATCG 
     2041 GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT GGGAGTCAGA 
     2101 TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC CTTCACGAAG 
     2161 GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA ACGGATGCTC 
     2221 GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA GACAGCCAGC 
     2281 AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA GATATTTCAG 
     2341 AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT TCTGATTATG 
     2401 ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG GACACGCCGA 
     2461 GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG GATATTGCAG 
     2521 GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC CCAGCTCCTC 
     2581 AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA GCAATATATG 
     2641 GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG 
     2701 GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG GCGGAGCAGG 
     2761 AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC AGAGGATTAG 
     2821 TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG GTGACCATTT 
     2881 TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA GGGGATTCGT 
     2941 TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG GCCTTCACCA 
     3001 GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC AACTGGCTGG 
     3061 CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC AGAGCTGAAC 
     3121 AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA GATTTCTGGA 
     3181 GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG GAAGACCTCA 
     3241 CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG CTGCGAGAAG 
     3301 CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA ATGGAAAAAT 
     3361 TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA GATGCAGGAT 
     3421 CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT GAACAGGATA 
     3481 AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC ACCCAGTGGT 
     3541 TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC ATGAGACAAA 
     3601 GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC AACAAGCTTA 
     3661 AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC AATGATAGCT 
     3721 GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT TGGGTCTCTG 
     3781 AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC TTAACCGCCA 
     3841 TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA GACACGGACG 
     3901 GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG CCGCTGGTGT 
     3961 CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG AAACCCAGCC 
     4021 CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG GACAATAGCC 
     4081 CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA GAAGAATCCT 
     4141 ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT GGTAATGAAA 
     4201 CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT ACCTTTGGGC 
     4261 GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG GTGGGAGAGA 
     4321 GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA ATATTTGAGA 
     4381 AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA CAGAATGCAA 
     4441 GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA ACGCACAAGC 
     4501 CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG AAAGCTCCTT 
     4561 CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG GAGGAGTACC 
     4621 GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC CGATACCGGG 
     4681 ACACAGAATT AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4741 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4801 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4861 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4921 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4981 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5041 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5101 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5161 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5221 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5281 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5341 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5401 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5461 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5521 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5581 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5641 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5701 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5761 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5821 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5881 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5941 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6001 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6061 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6121 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6181 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6241 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6301 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6361 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6421 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6481 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6541 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6601 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6661 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6721 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6781 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6841 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6901 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6961 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7021 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7081 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7141 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7201 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7261 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7321 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7381 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7441 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7501 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7561 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7621 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7681 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7741 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7801 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7861 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7921 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7981 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8041 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8101 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8161 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8221 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8281 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8341 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8401 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8461 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8521 TAACAAAAAT TTAACGCGAA TTTT
//