LOCUS       RC230676                8535 bp    DNA     circular  SYN 
DEFINITION  AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 1
ACCESSION   NM_001166693
VERSION     NM_001166693.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4692..4783 
                     /label="Myc-DDK"  
     misc_feature    4813..5398 
                     /label="PolyA signal"  
     misc_feature    4870..4889 
                     /label="Restriction Enzyme"  
     misc_feature    5674..6346 
                     /label="ColE1"  
     misc_feature    6776..7570 
                     /label="Kan/Neo"  
     misc_feature    7605..7907 
                     /label="SV40 ori"  
     misc_feature    7993..8470 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4674..4680 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4674 
                     /label="AFF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATTTACA GAAAGAGTCA ACAGCAGTGG CAACAGTTTG TACAATGACG 
     1081 ACAGAAACCT GCTTCGAATT AGAGAGAAGG AAAGACGCAA CCAGGAAGCC CACCAAGAGA 
     1141 AAGAGGCATT TCCTGAAAAG ATTCCCCTTT TTGGAGAGCC CTACAAGACA GCAAAAGGTG 
     1201 ATGAGCTGTC TAGTCGAATA CAGAACATGT TGGGAAACTA CGAAGAAGTG AAGGAGTTCC 
     1261 TTAGTACTAA GTCTCACACT CATCGCCTGG ATGCTTCTGA AAATAGGTTG GGAAAGCCGA 
     1321 AATATCCTTT AATTCCTGAC AAAGGGAGCA GCATTCCATC CAGCTCCTTC CACACTAGTG 
     1381 TCCACCACCA GTCCATTCAC ACTCCTGCGT CTGGACCACT TTCTGTTGGC AACATTAGCC 
     1441 ACAATCCAAA GATGGCGCAG CCAAGAACTG AACCAATGCC AAGTCTCCAT GCCAAAAGCT 
     1501 GCGGCCCACC GGACAGCCAG CACCTGACCC AGGATCGCCT TGGTCAGGAG GGGTTCGGCT 
     1561 CTAGTCATCA CAAGAAAGGT GACCGAAGAG CTGACGGAGA CCACTGTGCT TCGGTGACAG 
     1621 ATTCGGCTCC AGAGAGGGAG CTTTCTCCCT TAATCTCTTT GCCTTCCCCA GTTCCCCCTT 
     1681 TGTCACCTAT ACATTCCAAC CAGCAAACTC TTCCCCGGAC GCAAGGAAGC AGCAAGGTTC 
     1741 ATGGCAGCAG CAATAACAGT AAAGGCTATT GCCCAGCCAA ATCTCCCAAG GACCTAGCAG 
     1801 TGAAAGTCCA TGATAAAGAG ACCCCTCAAG ACAGTTTGGT GGCCCCTGCC CAGCCGCCTT 
     1861 CTCAGACATT TCCACCTCCC TCCCTCCCCT CAAAAAGTGT TGCAATGCAG CAGAAGCCCA 
     1921 CGGCTTATGT CCGGCCCATG GATGGTCAAG ATCAGGCCCC TAGTGAATCC CCTGAACTGA 
     1981 AACCACTGCC GGAGGACTAT CGACAGCAGA CCTTTGAAAA AACAGACTTG AAAGTGCCTG 
     2041 CCAAAGCCAA GCTCACCAAA CTGAAGATGC CTTCTCAGTC AGTTGAGCAG ACCTACTCCA 
     2101 ATGAAGTCCA TTGTGTTGAA GAGATTCTGA AGGAAATGAC CCATTCATGG CCGCCTCCTT 
     2161 TGACAGCAAT ACATACGCCT AGTACAGCTG AGCCATCCAA GTTTCCTTTC CCTACAAAGG 
     2221 ACTCTCAGCA TGTCAGTTCT GTAACCCAAA ACCAAAAACA ATATGATACA TCTTCAAAAA 
     2281 CTCACTCAAA TTCTCAGCAA GGAACGTCAT CCATGCTCGA AGACGACCTT CAGCTCAGTG 
     2341 ACAGTGAGGA CAGTGACAGT GAACAAACCC CAGAGAAGCC TCCCTCCTCA TCTGCACCTC 
     2401 CAAGTGCTCC ACAGTCCCTT CCAGAACCAG TGGCATCAGC ACATTCCAGC AGTGCAGAGT 
     2461 CAGAAAGCAC CAGTGACTCA GACAGTTCCT CAGACTCAGA GAGCGAGAGC AGTTCAAGTG 
     2521 ACAGCGAAGA AAATGAGCCC CTAGAAACCC CAGCTCCGGA GCCTGAGCCT CCAACAACAA 
     2581 ACAAATGGCA GCTGGACAAC TGGCTGACCA AAGTCAGCCA GCCAGCTGCG CCACCAGAGG 
     2641 GCCCCAGGAG CACAGAGCCC CCACGGCGGC ACCCAGAGAG TAAGGGCAGC AGCGACAGTG 
     2701 CCACGAGTCA GGAGCATTCT GAATCCAAAG ATCCTCCCCC TAAAAGCTCC AGCAAAGCCC 
     2761 CCCGGGCCCC ACCCGAAGCC CCCCACCCCG GAAAGAGGAG CTGTCAGAAG TCTCCGGCAC 
     2821 AGCAGGAGCC CCCACAAAGG CAAACCGTTG GAACCAAACA ACCCAAAAAA CCTGTCAAGG 
     2881 CCTCTGCCCG GGCAGGTTCA CGGACCAGCC TGCAGGGGGA AAGGGAGCCA GGGCTTCTTC 
     2941 CCTATGGCTC CCGAGACCAG ACTTCCAAAG ACAAGCCCAA GGTGAAGACG AAAGGACGGC 
     3001 CCCGGGCCGC AGCAAGCAAC GAACCCAAGC CAGCAGTGCC CCCCTCCAGT GAGAAGAAGA 
     3061 AGCACAAGAG CTCCCTCCCT GCCCCCTCTA AGGCTCTCTC AGGCCCAGAA CCCGCGAAGG 
     3121 ACAATGTGGA GGACAGGACC CCTGAGCACT TTGCTCTTGT TCCCCTGACT GAGAGCCAGG 
     3181 GCCCACCCCA CAGTGGCAGC GGCAGCAGGA CTAGTGGCTG CCGCCAAGCC GTGGTGGTCC 
     3241 AGGAGGACAG CCGCAAAGAC AGACTCCCAT TGCCTTTGAG AGACACCAAG CTGCTCTCAC 
     3301 CGCTCAGGGA CACTCCTCCC CCACAAAGCT TGATGGTGAA GATCACCCTA GACCTGCTCT 
     3361 CTCGGATACC CCAGCCTCCC GGGAAGGGGA GCCGCCAGAG GAAAGCAGAA GATAAACAGC 
     3421 CGCCCGCAGG GAAGAAGCAC AGCTCTGAGA AGAGGAGCTC AGACAGCTCA AGCAAGTTGG 
     3481 CCAAAAAGAG AAAGGGTGAA GCAGAAAGAG ACTGTGATAA CAAGAAAATC AGACTGGAGA 
     3541 AGGAAATCAA ATCACAGTCA TCTTCATCTT CATCCTCCCA CAAAGAATCT TCTAAAACAA 
     3601 AGCCCTCCAG GCCCTCCTCA CAGTCCTCAA AGAAGGAAAT GCTCCCCCCG CCACCCGTGT 
     3661 CCTCGTCCTC CCAGAAGCCA GCCAAGCCTG CACTTAAGAG GTCAAGGCGG GAAGCAGACA 
     3721 CCTGTGGCCA GGACCCTCCC AAAAGTGCCA GCAGTACCAA GAGCAACCAC AAAGACTCTT 
     3781 CCATTCCCAA GCAGAGAAGA GTAGAGGGGA AGGGCTCCAG AAGCTCCTCG GAGCACAAGG 
     3841 GTTCTTCCGG AGATACTGCA AATCCTTTTC CAGTGCCTTC TTTGCCAAAT GGTAACTCTA 
     3901 AACCAGGGAA GCCTCAAGTG AAGTTTGACA AACAACAAGC AGACCTTCAC ATGAGGGAGG 
     3961 CAAAAAAGAT GAAGCAGAAA GCAGAGTTAA TGACGGACAG GGTTGGAAAG GCTTTTAAGT 
     4021 ACCTGGAAGC CGTCTTGTCC TTCATTGAGT GCGGAATTGC CACAGAGTCT GAAAGCCAGT 
     4081 CATCCAAGTC AGCTTACTCT GTCTACTCAG AAACTGTAGA TCTCATTAAA TTCATAATGT 
     4141 CATTAAAATC CTTCTCAGAT GCCACAGCGC CAACACAAGA GAAAATATTT GCTGTTTTAT 
     4201 GCATGCGTTG CCAGTCCATT TTGAACATGG CGATGTTTCG TTGTAAAAAA GACATAGCAA 
     4261 TAAAGTATTC TCGTACTCTT AATAAACACT TCGAGAGTTC TTCCAAAGTC GCCCAGGCAC 
     4321 CTTCTCCATG CATTGCAAGA AGCACAGGCA CACCATCCCC TCTTTCCCCA ATGCCTTCTC 
     4381 CTGCCAGCTC CGTAGGGTCC CAGTCAAGTG CTGGCAGTGT GGGGAGCAGT GGGGTGGCTG 
     4441 CCACTATCAG CACCCCAGTC ACCATCCAGA ATATGACATC TTCCTATGTC ACCATCACAT 
     4501 CCCATGTTCT TACCGCCTTT GACCTTTGGG AACAGGCCGA GGCCCTCACG AGGAAGAATA 
     4561 AAGAATTCTT TGCTCGGCTC AGCACAAATG TGTGCACCTT GGCCCTCAAC AGCAGTTTGG 
     4621 TGGACCTGGT GCACTATACA CGACAGGGTT TTCAGCAGCT ACAAGAATTA ACCAAAACAC 
     4681 CTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4741 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4801 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4861 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4921 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4981 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5041 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5101 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5161 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5221 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5281 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5341 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5401 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5461 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5521 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5581 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5641 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5701 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5761 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5821 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5881 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5941 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6001 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6061 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6121 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6181 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6241 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6301 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6361 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6421 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6481 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6541 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6601 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6661 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6721 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6781 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6841 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6901 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6961 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7021 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7081 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7141 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7201 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7261 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7321 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7381 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7441 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7501 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7561 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7621 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7681 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7741 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7801 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7861 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7921 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7981 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8041 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8101 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8161 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8221 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8281 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8341 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8401 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8461 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8521 TTTAACGCGA ATTTT
//