LOCUS       RC230672                8451 bp    DNA     circular  SYN 
DEFINITION  ZEB2 (Myc-DDK-tagged)-Human zinc finger E-box binding homeobox 2 (ZEB2), transcript variant 2
ACCESSION   NM_001171653
VERSION     NM_001171653.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4608..4699 
                     /label="Myc-DDK"  
     misc_feature    4729..5314 
                     /label="PolyA signal"  
     misc_feature    4786..4805 
                     /label="Restriction Enzyme"  
     misc_feature    5590..6262 
                     /label="ColE1"  
     misc_feature    6692..7486 
                     /label="Kan/Neo"  
     misc_feature    7521..7823 
                     /label="SV40 ori"  
     misc_feature    7909..8386 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4590..4596 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4590 
                     /label="ZEB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCAGCCG ATCATGGCGG ATGGCCCCCG GTGCAAGAGG CGCAAACAAG 
     1081 CCAATCCCAG GAGGAAAAAC GTGGTGAACT ATGACAATGT AGTGGACACA GGTTCTGAAA 
     1141 CAGATGAGGA AGACAAGCTT CATATTGCTG AGGATGACGG TATTGCCAAC CCTCTGGACC 
     1201 AGGAGACGAG TCCAGCTAGT GTGCCCAACC ATGAGTCCTC CCCACACGTG AGCCAAGCTC 
     1261 TGTTGCCAAG AGAGGAAGAG GAAGATGAAA TAAGGGAGGG TGGAGTGGAA CACCCCTGGC 
     1321 ACAACAACGA GATTCTACAA GCCTCTGTAG ATGGTCCAGT GAAGAATGCA AATTGCACAT 
     1381 CAGATTTTGA GGAATACTTT GCCAAAAGAA AACTGGAGGA ACGCGATGGT CATGCAGTCA 
     1441 GCATCGAGGA GTACCTTCAG CGCAGTGACA CAGCCATTAT TTACCCAGAA GCCCCTGAGG 
     1501 AGCTGTCTCG CCTTGGCACG CCAGAGGCCA ATGGGCAAGA AGAAAATGAC CTGCCACCTG 
     1561 GAACTCCAGA TGCTTTTGCC CAACTGCTGA CCTGCCCCTA CTGCGACCGG GGCTACAAGC 
     1621 GCTTGACATC ACTGAAGGAG CACATCAAGT ACCGCCACGA GAAGAATGAA GAGAACTTTT 
     1681 CCTGCCCTCT CTGTAGCTAC ACGTTTGCCT ACCGCACCCA GCTCGAGCGG CATATGGTGA 
     1741 CACACAAGCC AGGGACAGAT CAGCACCAAA TGCTAACCCA AGGAGCAGGT AATCGCAAGT 
     1801 TCAAATGCAC AGAGTGTGGC AAGGCCTTCA AATATAAACA CCATCTGAAA GAACACCTGC 
     1861 GAATTCACAG TGGTGAAAAA CCTTACGAGT GCCCAAACTG CAAGAAACGT TTCTCCCATT 
     1921 CTGGTTCCTA CAGTTCGCAC ATCAGCAGCA AGAAATGTAT TGGTTTAATC TCTGTAAATG 
     1981 GCCGAATGAG AAACAATATC AAGACGGGTT CTTCCCCTAA TTCTGTTTCT TCTTCTCCTA 
     2041 CTAATTCAGC CATTACCCAG TTAAGAAACA AGTTGGAGAA TGGAAAACCA CTTAGTATGT 
     2101 CTGAACAGAC AGGCTTACTT AAAATTAAAA CAGAACCACT AGACTTCAAT GACTATAAAG 
     2161 TTCTTATGGC TACACACGGG TTTAGTGGCA CTAGTCCCTT TATGAATGGT GGGCTTGGAG 
     2221 CCACCAGCCC TTTAGGAGTT CATCCATCTG CTCAGAGTCC AATGCAGCAC TTAGGTGTAG 
     2281 GGATGGAAGC CCCTTTACTT GGGTTTCCCA CCATGAATAG TAATTTAAGT GAGGTACAAA 
     2341 AGGTTCTACA GATTGTGGAC AATACTGTTT CCAGGCAAAA AATGGACTGC AAGGCTGAAG 
     2401 AAATTTCAAA GTTGAAAGGT TATCACATGA AGGATCCATG CTCTCAACCT GAGGAACAAG 
     2461 GAGTTACTTC TCCTAATATT CCGCCTGTCG GTCTTCCGGT AGTGAGTCAT AATGGTGCCA 
     2521 CTAAAAGTAT TATTGACTAT ACGTTGGAAA AAGTCAATGA AGCCAAAGCT TGCCTCCAGA 
     2581 GCTTGACTAC TGACTCAAGG AGACAGATCA GTAATATAAA GAAAGAGAAG CTACGTACTT 
     2641 TAATAGATTT GGTCACTGAT GACAAAATGA TTGAGAACCA CAACATATCC ACTCCATTTT 
     2701 CATGCCAGTT CTGTAAAGAA AGTTTTCCTG GCCCCATCCC TTTGCATCAG CATGAACGTT 
     2761 ACCTTTGTAA GATGAATGAA GAGATCAAGG CGGTCCTGCA GCCTCATGAA AACATAGTCC 
     2821 CCAACAAAGC CGGAGTTTTT GTTGATAATA AAGCCCTCCT CTTGTCATCT GTACTTTCTG 
     2881 AGAAAGGAAT GACAAGCCCC ATCAACCCAT ACAAGGACCA CATGTCTGTA CTCAAAGCAT 
     2941 ACTATGCTAT GAACATGGAG CCCAACTCCG ATGAACTGCT GAAAATTTCC ATTGCTGTGG 
     3001 GCCTTCCTCA GGAATTTGTG AAGGAATGGT TTGAACAACG AAAAGTCTAC CAGTACTCAA 
     3061 ATTCCAGGTC CCCATCCCTG GAAAGAAGCT CCAAGCCGTT AGCTCCCAAC AGTAACCCTC 
     3121 CCACAAAAGA CTCTTTATTA CCCAGGTCTC CTGTAAAACC TATGGACTCC ATAACATCAC 
     3181 CATCTATAGC AGAACTCCAC AACAGTGTTA CGAATTGTGA TCCTCCTCTC AGGCTAACAA 
     3241 AACCTTCCCA TTTTACCAAT ATTAAACCAG TTGAAAAATT GGACCACTCC AGGAGTAATA 
     3301 CTCCTTCTCC CTTAAATCTT TCCTCCACAT CTTCTAAAAA CTCCCACAGT AGTTCATACA 
     3361 CTCCAAACAG CTTCTCTTCT GAGGAGCTCC AGGCTGAGCC TTTAGACTTG TCATTACCAA 
     3421 AACAAATGAA AGAACCCAAA AGTATTATAG CCACAAAGAA CAAAACAAAA GCTAGTAGCA 
     3481 TCAGTTTAGA TCATAACAGT GTTTCTTCCT CATCTGAAAA CTCAGATGAG CCTCTGAACT 
     3541 TGACTTTTAT CAAGAAGGAA TTTTCAAATT CAAATAATCT GGACAACAAA AGCACTAACC 
     3601 CAGTGTTCAG CATGAACCCA TTTAGTGCCA AACCTTTATA CACAGCTCTT CCACCTCAAA 
     3661 GCGCATTTCC CCCTGCTACT TTCATGCCAC CAGTCCAGAC CAGTATTCCT GGGCTACGAC 
     3721 CATACCCAGG ACTGGATCAG ATGAGCTTCC TACCACATAT GGCCTACACC TACCCAACTG 
     3781 GAGCAGCTAC TTTTGCTGAT ATGCAGCAAA GGAGAAAGTA CCAGCGGAAA CAAGGATTTC 
     3841 AGGGAGAATT GCTTGATGGA GCACAAGACT ACATGTCAGG CCTAGATGAT ATGACAGACT 
     3901 CCGACTCCTG TCTGTCTCGC AAAAAGATCA AGAAGACAGA GAGTGGCATG TATGCATGTG 
     3961 ACTTATGTGA CAAGACATTC CAGAAAAGCA GTTCCCTTCT GCGACATAAA TACGAACACA 
     4021 CAGGAAAAAG ACCACATCAG TGTCAGATTT GTAAGAAAGC GTTTAAACAC AAGCACCACC 
     4081 TTATCGAGCA CTCAAGGCTT CACTCGGGCG AGAAGCCCTA TCAGTGTGAT AAATGTGGCA 
     4141 AGCGCTTCTC ACACTCGGGC TCGTACTCGC AGCACATGAA TCACAGGTAT TCCTACTGCA 
     4201 AGCGGGAGGC GGAGGAGCGG GAAGCGGCGG AGCGCGAGGC GCGCGAGAAA GGGCACTTGG 
     4261 AACCCACCGA GCTGCTGATG AACCGGGCTT ACTTGCAGAG CATTACCCCT CAGGGGTACT 
     4321 CTGACTCGGA GGAGAGGGAG AGTATGCCGA GGGATGGCGA GAGCGAGAAG GAGCACGAGA 
     4381 AAGAAGGCGA GGATGGCTAC GGGAAGCTGG GCAGACAGGA TGGCGACGAG GAGTTCGAGG 
     4441 AGGAAGAGGA AGAAAGTGAA AATAAAAGTA TGGATACGGA TCCCGAAACG ATACGAGATG 
     4501 AAGAAGAGAC TGGAGATCAC TCCATGGACG ATAGTTCGGA GGATGGGAAA ATGGAAACCA 
     4561 AATCAGACCA CGAGGAAGAC AATATGGAAG ATGGCATGAC GCGTACGCGG CCGCTCGAGC 
     4621 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4681 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4741 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4801 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4861 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4921 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4981 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5041 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5101 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5161 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5221 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5281 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5341 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5401 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5461 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5521 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5581 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5641 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5701 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5761 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5821 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5881 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5941 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6001 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6061 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6121 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6181 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6241 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6301 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6361 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6421 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6481 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6541 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6601 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6661 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6721 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6781 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6841 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6901 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6961 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7021 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7081 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7141 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7201 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7261 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7321 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7381 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7441 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7501 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7561 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7621 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7681 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7861 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7921 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7981 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8041 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8101 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8161 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8221 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8281 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8341 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8401 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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