LOCUS       RC230671                8442 bp    DNA     circular  SYN 
DEFINITION  PLCB4 (Myc-DDK-tagged)-Human phospholipase C, beta 4 (PLCB4), transcript variant 3
ACCESSION   NM_001172646
VERSION     NM_001172646.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4599..4690 
                     /label="Myc-DDK"  
     misc_feature    4720..5305 
                     /label="PolyA signal"  
     misc_feature    4777..4796 
                     /label="Restriction Enzyme"  
     misc_feature    5581..6253 
                     /label="ColE1"  
     misc_feature    6683..7477 
                     /label="Kan/Neo"  
     misc_feature    7512..7814 
                     /label="SV40 ori"  
     misc_feature    7900..8377 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4581..4587 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4581 
                     /label="PLCB4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAAACCT TATGAATTTA ACTGGCAGAA GGAAGTTCCC TCCTTTTTGC 
     1081 AAGAAGGAAC AGTTTTTGAC AGATACGAGG AGGAATCCTT TGTGTTTGAA CCCAACTGCC 
     1141 TCTTCAAAGT GGATGAGTTT GGCTTCTTTC TGACATGGAG AAGTGAAGGC AAGGAAGGAC 
     1201 AGGTGCTAGA ATGCTCCCTC ATCAACAGTA TTCGGTCGGG AGCCATACCA AAGGATCCCA 
     1261 AAATCTTGGC TGCTCTTGAA GCTGTTGGAA AATCAGAAAA TGATCTGGAA GGGCGGATAG 
     1321 TTTGTGTCTG CAGTGGCACA GATCTAGTGA ACATTAGTTT TACCTACATG GTGGCTGAAA 
     1381 ATCCAGAAGT AACTAAGCAA TGGGTAGAAG GCCTGAGATC AATCATACAC AACTTCAGGG 
     1441 CCAACAACGT CAGTCCAATG ACATGCCTCA AGAAACACTG GATGAAATTG GCATTTATGA 
     1501 CCAACACAAA TGGTAAAATT CCAGTTAGGA GTATTACTAG AACATTTGCA TCGGGAAAAA 
     1561 CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGTTAGGTCT TCCCAGTGGA AAGAATGATG 
     1621 AAATTGAGCC CACAGCATTT TCTTATGAAA AGTTCTATGA ACTGACACAA AAGATTTGTC 
     1681 CTCGGACAGA TATAGAAGAT CTTTTCAAAA AAATCAATGG AGACAAAACT GATTATTTAA 
     1741 CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA TTGAATGAAA 
     1801 TTTTATTTCC ATTTTATGAT GCCAAAAGGG CAATGCAGAT CATTGAGATG TATGAACCTG 
     1861 ATGAAGATTT GAAGAAAAAA GGCCTTATAT CAAGTGATGG GTTTTGCAGA TATCTGATGT 
     1921 CAGATGAAAA CGCCCCAGTC TTCCTAGATC GTTTAGAACT TTACCAAGAA ATGGACCATC 
     1981 CTCTGGCTCA CTACTTCATC AGTTCTTCCC ATAACACTTA TCTCACTGGC AGACAGTTCG 
     2041 GCGGGAAGTC TTCGGTAGAA ATGTACAGAC AGGTTCTCCT GGCTGGTTGC AGATGTGTTG 
     2101 AACTTGACTG CTGGGATGGA AAAGGTGAAG ACCAAGAACC AATAATAACT CATGGAAAAG 
     2161 CAATGTGTAC AGATATCCTT TTTAAGGATG TAATTCAAGC CATCAAGGAA ACTGCATTTG 
     2221 TCACATCAGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA TATCAACAGT 
     2281 ACAAGATGTC CAAATATTGC GAAGATCTAT TTGGGGATCT CCTGTTGAAA CAAGCACTTG 
     2341 AATCACATCC ACTTGAACCA GGCAGGGCTT TGCCATCCCC CAATGACCTC AAAAGAAAAA 
     2401 TACTCATAAA AAACAAGCGG CTGAAACCTG AAGTTGAAAA AAAACAGCTG GAAGCTTTGA 
     2461 GAAGCATGAT GGAAGCTGGA GAATCTGCCT CCCCAGCAAA CATCTTAGAG GACGATAATG 
     2521 AAGAGGAGAT CGAAAGTGCT GACCAAGAGG AGGAAGCTCA CCCCGAATTC AAATTTGGAA 
     2581 ATGAACTTTC TGCTGATGAC TTGGGTCACA AGGAAGCTGT TGCAAATAGC GTCAAGAAGG 
     2641 CTTCAGATGA CCTTGAACAT GAAAACAACA AAAAGGGCCT GGTCACTGTA GAAGATGAGC 
     2701 AGGCGTGGAT GGCATCTTAT AAATATGTAG GTGCTACCAC TAATATCCAT CCATATTTGT 
     2761 CCACAATGAT CAACTACGCC CAGCCTGTAA AGTTTCAAGG TTTCCATGTG GCAGAAGAAC 
     2821 GCAATATTCA TTATAACATG TCTTCTTTTA ATGAATCAGT CGGTCTTGGC TACTTGAAGA 
     2881 CACATGCAAT TGAATTTGTC AATTATAACA AACGGCAAAT GAGTCGCATT TACCCCAAGG 
     2941 GAGGCCGAGT CGATTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT GGCTGCCAGA 
     3001 TGGTTTCACT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG GGAAAATTTG 
     3061 AGTATAATGG ATCGTGCGGG TACCTTCTCA AACCAGATTT CATGAGGCGG CCTGATCGAA 
     3121 CATTTGACCC CTTCTCTGAA ACTCCTGTTG ATGGTGTTAT TGCAGCCACT TGCTCAGTGC 
     3181 AGGTTATATC AGGTCAATTC TTATCAGATA AGAAAATTGG CACCTACGTA GAGGTGGATA 
     3241 TGTATGGGTT GCCCACTGAC ACCATACGTA AGGAATTCCG AACTCGCATG GTTATGAATA 
     3301 ATGGACTCAA TCCAGTTTAC AATGAAGAGT CATTTGTATT TCGGAAGGTG ATCCTGCCGG 
     3361 ACCTGGCTGT CTTGAGAATA GCTGTGTATG ATGATAACAA CAAGCTGATT GGCCAGAGGA 
     3421 TCCTCCCGCT TGATGGCCTC CAAGCCGGAT ATCGACACAT TTCCCTTCGA AATGAGGGAA 
     3481 ATAAACCATT ATCACTACCA ACAATTTTCT GCAATATTGT TCTTAAAACA TATGTGCCTG 
     3541 ATGGATTTGG AGATATCGTG GATGCTTTAT CAGATCCAAA GAAATTTCTC TCAATTACAG 
     3601 AAAAGAGAGC AGACCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATA GCCGACGTGC 
     3661 CCAGTGACAC TTCCAAAAAT GACAAGAAAG GAAAGGCCAA CACCGCCAAA GCAAATGTGA 
     3721 CCCCTCAGAG TAGCTCTGAG CTCAGACCAA CCACCACGGC TGCCCTGGCC TCTGGTGTGG 
     3781 AAGCCAAGAA AGGTATTGAA CTTATCCCTC AAGTAAGGAT AGAAGACTTA AAGCAGATGA 
     3841 AGGCTTACTT GAAGCATTTA AAGAAACAGC AGAAGGAGCT AAATTCTTTA AAGAAGAAAC 
     3901 ATGCAAAGGA ACACAGTACC ATGCAGAAGT TACACTGCAC GCAAGTTGAC AAAATTGTGG 
     3961 CACAGTATGA CAAAGAGAAG TCGACTCATG AGAAAATCCT AGAGAAGGCA ATGAAGAAGA 
     4021 AGGGGGGAAG TAATTGTCTC GAAATGAAAA AAGAAACAGA AATCAAAATT CAGACGCTGA 
     4081 CATCAGATCA CAAATCTAAG GTCAAAGAGA TTGTAGCACA GCACACAAAG GAATGGTCAG 
     4141 AAATGATCAA TACCCACAGT GCTGAGGAGC AAGAAATCCG AGACCTGCAC CTCAGCCAGC 
     4201 AGTGTGAGCT GCTGAAAAAG CTACTCATCA ATGCCCACGA GCAGCAAACC CAGCAGCTGA 
     4261 AACTGTCCCA TGACAGGGAA AGCAAGGAAA TGCGAGCACA CCAGGCTAAG ATTTCTATGG 
     4321 AAAATAGCAA AGCCATCAGC CAAGATAAAT CTATCAAGAA TAAAGCAGAA CGGGAAAGGC 
     4381 GAGTCAGGGA GTTAAACAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA AAGAGACTTG 
     4441 CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTTGAA CATCTAGAAT 
     4501 TCCTAGAGAA ACAGAATGAG CAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG 
     4561 AGATGGACCG CAGACCAGCA ACAGTAGTAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4621 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4681 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4741 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4801 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4861 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4921 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4981 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5041 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5101 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5161 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5221 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5281 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5341 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5401 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5461 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5521 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5581 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5641 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5701 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5761 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5821 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5881 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5941 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6001 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6061 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6121 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6181 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6241 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6301 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6361 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6421 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6481 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6541 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6601 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6661 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6721 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6781 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6841 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6901 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6961 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7021 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7081 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7141 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7201 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7261 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7321 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7381 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7441 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7501 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7561 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7621 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7681 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7741 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7801 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7861 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7921 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7981 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8041 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8101 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8161 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8221 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8281 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8341 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8401 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//