LOCUS       RC230667                8379 bp    DNA     circular  SYN 
DEFINITION  EGF (Myc-DDK-tagged)-Human epidermal growth factor (EGF), transcript variant 2
ACCESSION   NM_001178130
VERSION     NM_001178130.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4536..4627 
                     /label="Myc-DDK"  
     misc_feature    4657..5242 
                     /label="PolyA signal"  
     misc_feature    4714..4733 
                     /label="Restriction Enzyme"  
     misc_feature    5518..6190 
                     /label="ColE1"  
     misc_feature    6620..7414 
                     /label="Kan/Neo"  
     misc_feature    7449..7751 
                     /label="SV40 ori"  
     misc_feature    7837..8314 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4518..4524 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4518 
                     /label="EGF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCTCACT CTTATCATTC TGTTGCCAGT AGTTTCAAAA TTTAGTTTTG 
     1081 TTAGTCTCTC AGCACCGCAG CACTGGAGCT GTCCTGAAGG TACTCTCGCA GGAAATGGGA 
     1141 ATTCTACTTG TGTGGGTCCT GCACCCTTCT TAATTTTCTC CCATGGAAAT AGTATCTTTA 
     1201 GGATTGACAC AGAAGGAACC AATTATGAGC AATTGGTGGT GGATGCTGGT GTCTCAGTGA 
     1261 TCATGGATTT TCATTATAAT GAGAAAAGAA TCTATTGGGT GGATTTAGAA AGACAACTTT 
     1321 TGCAAAGAGT TTTTCTGAAT GGGTCAAGGC AAGAGAGAGT ATGTAATATA GAGAAAAATG 
     1381 TTTCTGGAAT GGCAATAAAT TGGATAAATG AAGAAGTTAT TTGGTCAAAT CAACAGGAAG 
     1441 GAATCATTAC AGTAACAGAT ATGAAAGGAA ATAATTCCCA CATTCTTTTA AGTGCTTTAA 
     1501 AATATCCTGC AAATGTAGCA GTTGATCCAG TAGAAAGGTT TATATTTTGG TCTTCAGAGG 
     1561 TGGCTGGAAG CCTTTATAGA GCAGATCTCG ATGGTGTGGG AGTGAAGGCT CTGTTGGAGA 
     1621 CATCAGAGAA AATAACAGCT GTGTCATTGG ATGTGCTTGA TAAGCGGCTG TTTTGGATTC 
     1681 AGTACAACAG AGAAGGAAGC AATTCTCTTA TTTGCTCCTG TGATTATGAT GGAGGTTCTG 
     1741 TCCACATTAG TAAACATCCA ACACAGCATA ATTTGTTTGC AATGTCCCTT TTTGGTGACC 
     1801 GTATCTTCTA TTCAACATGG AAAATGAAGA CAATTTGGAT AGCCAACAAA CACACTGGAA 
     1861 AGGACATGGT TAGAATTAAC CTCCATTCAT CATTTGTACC ACTTGGTGAA CTGAAAGTAG 
     1921 TGCATCCACT TGCACAACCC AAGGCAGAAG ATGACACTTG GGAGCCTGAG CAGAAACTTT 
     1981 GCAAATTGAG GAAAGGAAAC TGCAGCAGCA CTGTGTGTGG GCAAGACCTC CAGTCACACT 
     2041 TGTGCATGTG TGCAGAGGGA TACGCCCTAA GTCGAGACCG GAAGTACTGT GAAGATGTTA 
     2101 ATGAATGTGC TTTTTGGAAT CATGGCTGTA CTCTTGGGTG TAAAAACACC CCTGGATCCT 
     2161 ATTACTGCAC GTGCCCTGTA GGATTTGTTC TGCTTCCTGA TGGGAAACGA TGTCATCAAC 
     2221 TTGTTTCCTG TCCACGCAAT GTGTCTGAAT GCAGCCATGA CTGTGTTCTG ACATCAGAAG 
     2281 GTCCCTTATG TTTCTGTCCT GAAGGCTCAG TGCTTGAGAG AGATGGGAAA ACATGTAGCG 
     2341 GTTGTTCCTC ACCCGATAAT GGTGGATGTA GCCAGCTCTG CGTTCCTCTT AGCCCAGTAT 
     2401 CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAACT GGATGAAAAA AGCTGTGCAG 
     2461 CTTCAGGACC ACAACCATTT TTGCTGTTTG CCAATTCTCA AGATATTCGA CACATGCATT 
     2521 TTGATGGAAC AGACTATGGA ACTCTGCTCA GCCAGCAGAT GGGAATGGTT TATGCCCTAG 
     2581 ATCATGACCC TGTGGAAAAT AAGATATACT TTGCCCATAC AGCCCTGAAG TGGATAGAGA 
     2641 GAGCTAATAT GGATGGTTCC CAGCGAGAAA GGCTTATTGA GGAAGGAGTA GATGTGCCAG 
     2701 AAGGTCTTGC TGTGGACTGG ATTGGCCGTA GATTCTATTG GACAGACAGA GGGAAATCTC 
     2761 TGATTGGAAG GAGTGATTTA AATGGGAAAC GTTCCAAAAT AATCACTAAG GAGAACATCT 
     2821 CTCAACCACG AGGAATTGCT GTTCATCCAA TGGCCAAGAG ATTATTCTGG ACTGATACAG 
     2881 GGATTAATCC ACGAATTGAA AGTTCTTCCC TCCAAGGCCT TGGCCGTCTG GTTATAGCCA 
     2941 GCTCTGATCT AATCTGGCCC AGTGGAATAA CGATTGACTT CTTAACTGAC AAGTTGTACT 
     3001 GGTGCGATGC CAAGCAGTCT GTGATTGAAA TGGCCAATCT GGATGGTTCA AAACGCCGAA 
     3061 GACTTACCCA GAATGATGTA GGTCACCCAT TTGCTGTAGC AGTGTTTGAG GATTATGTGT 
     3121 GGTTCTCAGA TTGGGCTATG CCATCAGTAA TGAGAGTAAA CAAGAGGACT GGCAAAGATA 
     3181 GAGTACGTCT CCAAGGCAGC ATGCTGAAGC CCTCATCACT GGTTGTGGTT CATCCATTGG 
     3241 CAAAACCAGG AGCAGATCCC TGCTTATATC AAAACGGAGG CTGTGAACAT ATTTGCAAAA 
     3301 AGAGGCTTGG AACTGCTTGG TGTTCGTGTC GTGAAGGTTT TATGAAAGCC TCAGATGGGA 
     3361 AAACGTGTCT GGCTCTGGAT GGTCATCAGC TGTTGGCAGG TGGTGAAGTT GATCTAAAGA 
     3421 ACCAAGTAAC ACCATTGGAC ATCTTGTCCA AGACTAGAGT GTCAGAAGAT AACATTACAG 
     3481 AATCTCAACA CATGCTAGTG GCTGAAATCA TGGTGTCAGA TCAAGATGAC TGTGCTCCTG 
     3541 TGGGATGCAG CATGTATGCT CGGTGTATTT CAGAGGGAGA GGATGCCACA TGTCAGTGTT 
     3601 TGAAAGGATT TGCTGGGGAT GGAAAACTAT GTTCTGATAT AGATGAATGT GAGATGGGTG 
     3661 TCCCAGTGTG CCCCCCTGCC TCCTCCAAGT GCATCAACAC CGAAGGTGGT TATGTCTGCC 
     3721 GGTGCTCAGA AGGCTACCAA GGAGATGGGA TTCACTGTCT TGACTCTACT CCACCCCCTC 
     3781 ACCTCAGGGA AGATGACCAC CACTATTCCG TAAGAAATAG TGACTCTGAA TGTCCCCTGT 
     3841 CCCACGATGG GTACTGCCTC CATGATGGTG TGTGCATGTA TATTGAAGCA TTGGACAAGT 
     3901 ATGCATGCAA CTGTGTTGTT GGCTACATCG GGGAGCGATG TCAGTACCGA GACCTGAAGT 
     3961 GGTGGGAACT GCGCCACGCT GGCCACGGGC AGCAGCAGAA GGTCATCGTG GTGGCTGTCT 
     4021 GCGTGGTGGT GCTTGTCATG CTGCTCCTCC TGAGCCTGTG GGGGGCCCAC TACTACAGGA 
     4081 CTCAGAAGCT GCTATCGAAA AACCCAAAGA ATCCTTATGA GGAGTCGAGC AGAGATGTGA 
     4141 GGAGTCGCAG GCCTGCTGAC ACTGAGGATG GGATGTCCTC TTGCCCTCAA CCTTGGTTTG 
     4201 TGGTTATAAA AGAACACCAA GACCTCAAGA ATGGGGGTCA ACCAGTGGCT GGTGAGGATG 
     4261 GCCAGGCAGC AGATGGGTCA ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG 
     4321 GAATGGGCAC AGAGCAAGGC TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC 
     4381 AGGTAATGGA GCGAAGCTTT CATATGCCCT CCTATGGGAC ACAGACCCTT GAAGGGGGTG 
     4441 TCGAGAAGCC CCATTCTCTC CTATCAGCTA ACCCATTATG GCAACAAAGG GCCCTGGACC 
     4501 CACCACACCA AATGGAGCTG ACTCAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4561 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5341 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5401 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5461 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5521 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5581 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5641 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5701 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5761 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5821 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5881 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5941 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6001 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6061 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6121 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6181 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6241 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6301 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6361 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6421 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6481 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6541 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6601 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6661 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6721 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6781 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6841 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6901 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6961 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7021 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7081 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7141 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7201 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7261 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7321 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7381 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7441 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7501 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7561 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7621 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7681 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7801 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7861 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7921 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7981 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8041 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8101 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8161 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8221 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8281 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8341 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//