LOCUS       RC230666                8376 bp    DNA     circular  SYN 
DEFINITION  EGF (Myc-DDK-tagged)-Human epidermal growth factor (EGF), transcript variant 3
ACCESSION   NM_001178131
VERSION     NM_001178131.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4533..4624 
                     /label="Myc-DDK"  
     misc_feature    4654..5239 
                     /label="PolyA signal"  
     misc_feature    4711..4730 
                     /label="Restriction Enzyme"  
     misc_feature    5515..6187 
                     /label="ColE1"  
     misc_feature    6617..7411 
                     /label="Kan/Neo"  
     misc_feature    7446..7748 
                     /label="SV40 ori"  
     misc_feature    7834..8311 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4515..4521 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4515 
                     /label="EGF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCTCACT CTTATCATTC TGTTGCCAGT AGTTTCAAAA TTTAGTTTTG 
     1081 TTAGTCTCTC AGCACCGCAG CACTGGAGCT GTCCTGAAGG TACTCTCGCA GGAAATGGGA 
     1141 ATTCTACTTG TGTGGGTCCT GCACCCTTCT TAATTTTCTC CCATGGAAAT AGTATCTTTA 
     1201 GGATTGACAC AGAAGGAACC AATTATGAGC AATTGGTGGT GGATGCTGGT GTCTCAGTGA 
     1261 TCATGGATTT TCATTATAAT GAGAAAAGAA TCTATTGGGT GGATTTAGAA AGACAACTTT 
     1321 TGCAAAGAGT TTTTCTGAAT GGGTCAAGGC AAGAGAGAGT ATGTAATATA GAGAAAAATG 
     1381 TTTCTGGAAT GGCAATAAAT TGGATAAATG AAGAAGTTAT TTGGTCAAAT CAACAGGAAG 
     1441 GAATCATTAC AGTAACAGAT ATGAAAGGAA ATAATTCCCA CATTCTTTTA AGTGCTTTAA 
     1501 AATATCCTGC AAATGTAGCA GTTGATCCAG TAGAAAGGTT TATATTTTGG TCTTCAGAGG 
     1561 TGGCTGGAAG CCTTTATAGA GCAGATCTCG ATGGTGTGGG AGTGAAGGCT CTGTTGGAGA 
     1621 CATCAGAGAA AATAACAGCT GTGTCATTGG ATGTGCTTGA TAAGCGGCTG TTTTGGATTC 
     1681 AGTACAACAG AGAAGGAAGC AATTCTCTTA TTTGCTCCTG TGATTATGAT GGAGGTTCTG 
     1741 TCCACATTAG TAAACATCCA ACACAGCATA ATTTGTTTGC AATGTCCCTT TTTGGTGACC 
     1801 GTATCTTCTA TTCAACATGG AAAATGAAGA CAATTTGGAT AGCCAACAAA CACACTGGAA 
     1861 AGGACATGGT TAGAATTAAC CTCCATTCAT CATTTGTACC ACTTGGTGAA CTGAAAGTAG 
     1921 TGCATCCACT TGCACAACCC AAGGCAGAAG ATGACACTTG GGAGCCTGAT GTTAATGAAT 
     1981 GTGCTTTTTG GAATCATGGC TGTACTCTTG GGTGTAAAAA CACCCCTGGA TCCTATTACT 
     2041 GCACGTGCCC TGTAGGATTT GTTCTGCTTC CTGATGGGAA ACGATGTCAT CAACTTGTTT 
     2101 CCTGTCCACG CAATGTGTCT GAATGCAGCC ATGACTGTGT TCTGACATCA GAAGGTCCCT 
     2161 TATGTTTCTG TCCTGAAGGC TCAGTGCTTG AGAGAGATGG GAAAACATGT AGCGGTTGTT 
     2221 CCTCACCCGA TAATGGTGGA TGTAGCCAGC TCTGCGTTCC TCTTAGCCCA GTATCCTGGG 
     2281 AATGTGATTG CTTTCCTGGG TATGACCTAC AACTGGATGA AAAAAGCTGT GCAGCTTCAG 
     2341 GACCACAACC ATTTTTGCTG TTTGCCAATT CTCAAGATAT TCGACACATG CATTTTGATG 
     2401 GAACAGACTA TGGAACTCTG CTCAGCCAGC AGATGGGAAT GGTTTATGCC CTAGATCATG 
     2461 ACCCTGTGGA AAATAAGATA TACTTTGCCC ATACAGCCCT GAAGTGGATA GAGAGAGCTA 
     2521 ATATGGATGG TTCCCAGCGA GAAAGGCTTA TTGAGGAAGG AGTAGATGTG CCAGAAGGTC 
     2581 TTGCTGTGGA CTGGATTGGC CGTAGATTCT ATTGGACAGA CAGAGGGAAA TCTCTGATTG 
     2641 GAAGGAGTGA TTTAAATGGG AAACGTTCCA AAATAATCAC TAAGGAGAAC ATCTCTCAAC 
     2701 CACGAGGAAT TGCTGTTCAT CCAATGGCCA AGAGATTATT CTGGACTGAT ACAGGGATTA 
     2761 ATCCACGAAT TGAAAGTTCT TCCCTCCAAG GCCTTGGCCG TCTGGTTATA GCCAGCTCTG 
     2821 ATCTAATCTG GCCCAGTGGA ATAACGATTG ACTTCTTAAC TGACAAGTTG TACTGGTGCG 
     2881 ATGCCAAGCA GTCTGTGATT GAAATGGCCA ATCTGGATGG TTCAAAACGC CGAAGACTTA 
     2941 CCCAGAATGA TGTAGGTCAC CCATTTGCTG TAGCAGTGTT TGAGGATTAT GTGTGGTTCT 
     3001 CAGATTGGGC TATGCCATCA GTAATGAGAG TAAACAAGAG GACTGGCAAA GATAGAGTAC 
     3061 GTCTCCAAGG CAGCATGCTG AAGCCCTCAT CACTGGTTGT GGTTCATCCA TTGGCAAAAC 
     3121 CAGGAGCAGA TCCCTGCTTA TATCAAAACG GAGGCTGTGA ACATATTTGC AAAAAGAGGC 
     3181 TTGGAACTGC TTGGTGTTCG TGTCGTGAAG GTTTTATGAA AGCCTCAGAT GGGAAAACGT 
     3241 GTCTGGCTCT GGATGGTCAT CAGCTGTTGG CAGGTGGTGA AGTTGATCTA AAGAACCAAG 
     3301 TAACACCATT GGACATCTTG TCCAAGACTA GAGTGTCAGA AGATAACATT ACAGAATCTC 
     3361 AACACATGCT AGTGGCTGAA ATCATGGTGT CAGATCAAGA TGACTGTGCT CCTGTGGGAT 
     3421 GCAGCATGTA TGCTCGGTGT ATTTCAGAGG GAGAGGATGC CACATGTCAG TGTTTGAAAG 
     3481 GATTTGCTGG GGATGGAAAA CTATGTTCTG ATATAGATGA ATGTGAGATG GGTGTCCCAG 
     3541 TGTGCCCCCC TGCCTCCTCC AAGTGCATCA ACACCGAAGG TGGTTATGTC TGCCGGTGCT 
     3601 CAGAAGGCTA CCAAGGAGAT GGGATTCACT GTCTTGATAT TGATGAGTGC CAACTGGGGG 
     3661 AGCACAGCTG TGGAGAGAAT GCCAGCTGCA CAAATACAGA GGGAGGCTAT ACCTGCATGT 
     3721 GTGCTGGACG CCTGTCTGAA CCAGGACTGA TTTGCCCTGA CTCTACTCCA CCCCCTCACC 
     3781 TCAGGGAAGA TGACCACCAC TATTCCGTAA GAAATAGTGA CTCTGAATGT CCCCTGTCCC 
     3841 ACGATGGGTA CTGCCTCCAT GATGGTGTGT GCATGTATAT TGAAGCATTG GACAAGTATG 
     3901 CATGCAACTG TGTTGTTGGC TACATCGGGG AGCGATGTCA GTACCGAGAC CTGAAGTGGT 
     3961 GGGAACTGCG CCACGCTGGC CACGGGCAGC AGCAGAAGGT CATCGTGGTG GCTGTCTGCG 
     4021 TGGTGGTGCT TGTCATGCTG CTCCTCCTGA GCCTGTGGGG GGCCCACTAC TACAGGACTC 
     4081 AGAAGCTGCT ATCGAAAAAC CCAAAGAATC CTTATGAGGA GTCGAGCAGA GATGTGAGGA 
     4141 GTCGCAGGCC TGCTGACACT GAGGATGGGA TGTCCTCTTG CCCTCAACCT TGGTTTGTGG 
     4201 TTATAAAAGA ACACCAAGAC CTCAAGAATG GGGGTCAACC AGTGGCTGGT GAGGATGGCC 
     4261 AGGCAGCAGA TGGGTCAATG CAACCAACTT CATGGAGGCA GGAGCCCCAG TTATGTGGAA 
     4321 TGGGCACAGA GCAAGGCTGC TGGATTCCAG TATCCAGTGA TAAGGGCTCC TGTCCCCAGG 
     4381 TAATGGAGCG AAGCTTTCAT ATGCCCTCCT ATGGGACACA GACCCTTGAA GGGGGTGTCG 
     4441 AGAAGCCCCA TTCTCTCCTA TCAGCTAACC CATTATGGCA ACAAAGGGCC CTGGACCCAC 
     4501 CACACCAAAT GGAGCTGACT CAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4561 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4621 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4681 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4741 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4801 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4861 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4921 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4981 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5041 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5101 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5161 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5221 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5281 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5341 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5401 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5461 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5521 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5581 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5641 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5701 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5761 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5821 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5881 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5941 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6001 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6061 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6121 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6181 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6241 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6301 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6361 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6421 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6481 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6541 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6601 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6661 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6721 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6781 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6841 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6901 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6961 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7021 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7081 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7141 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7201 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7261 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7321 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7381 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7441 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7501 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7561 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7621 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7801 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7861 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7921 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7981 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8041 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8101 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8161 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8221 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8281 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8341 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//