LOCUS       RC230664                8352 bp    DNA     circular  SYN 
DEFINITION  TJP2 (Myc-DDK-tagged)-Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 4
ACCESSION   NM_001170415
VERSION     NM_001170415.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4509..4600 
                     /label="Myc-DDK"  
     misc_feature    4630..5215 
                     /label="PolyA signal"  
     misc_feature    4687..4706 
                     /label="Restriction Enzyme"  
     misc_feature    5491..6163 
                     /label="ColE1"  
     misc_feature    6593..7387 
                     /label="Kan/Neo"  
     misc_feature    7422..7724 
                     /label="SV40 ori"  
     misc_feature    7810..8287 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4491..4497 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4491 
                     /label="TJP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGACTGCT CAAGCCCTAC ATAGGATGTG GATCCAGGCT GTTAAAAAGT 
     1081 TGAGGAGATG GAAAGGCCGT GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG 
     1141 TGACCCTACA AAAGGATTCC AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA 
     1201 ACCCCCACTT TGAAAATGGA GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC 
     1261 CTGCTGATGG GCTGCTCCAA GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG 
     1321 AGGATGTGCT TCATTCGTTT GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA 
     1381 TTGTGGTCAA GAGGCCCCGG AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG 
     1441 ATCAGGATGA CCGGGCTTTT GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG 
     1501 GCTACAGCGA GAGGAGCCGG CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA 
     1561 GCCCGGAAAG GGGGCGTCCC CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG 
     1621 ACCGGAGCCG TGGCCGGAGC CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG 
     1681 ACCGCAGCCG TGGCCGGAGC CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG 
     1741 ACCGGGACCG TGACCGCAGC CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC 
     1801 ACCGGGCCTA CGACCCAGAC TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC 
     1861 GCCACGATGC CCGCTCTCGG GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA 
     1921 GCCCCAGCCC CGAGCCTAGG GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA 
     1981 GAGCGAACGA AGAGTATGGT CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC 
     2041 GAACGGGTCT GGCAACTAAA GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA 
     2101 ATGGGACTGT AACTGAGAAC ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA 
     2161 GAGGAAAACT ACAGCTAGTG GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT 
     2221 CATTAAATGA CAGTGACTCA GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT 
     2281 TTTCTCCAGA GGAGAGACGT CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA 
     2341 AGCTGAAGGA AAGGCCAAGT TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG 
     2401 CGACACCCAC TCCCTTTAAG TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA 
     2461 ACAAGGAACC CAGATACCAA GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA 
     2521 CTTTTCTTCG TCCTAGTCCT GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA 
     2581 GGTTCAAGAA GGGAGACAGC GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT 
     2641 TTGTTGCTGG CATTCAAGAA GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC 
     2701 AGATTCTGAA GGTGAACACA CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT 
     2761 ACCTGTTAGA AATCCCTAAA GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG 
     2821 TGTATAGAGA CATCCTGGCT TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG 
     2881 AATGTGAGAA GGAAACTCCA CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG 
     2941 TAGACACACT GTATGACGGC AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT 
     3001 TGGAGAAAGG CTTAATCCCC AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG 
     3061 CCCAGAGAGA CAACGCTGGG GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG 
     3121 GGGTGAAGAA GAACCTGAGG AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA 
     3181 CCAAGTTCCC AGCTTATGAG AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG 
     3241 TCTTATTCGG CCCCATAGCT GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT 
     3301 GGTTTCAAAC TGCTAAAACG GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG 
     3361 TCCGGTTAAA TACCGTGAGG CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA 
     3421 CTCCGAAAGC TGTGGACCTG TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA 
     3481 ACCCAGACTC CAGACAAGGT GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA 
     3541 AAAGTTCTCG AAAGTTATTT GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT 
     3601 TTACAGCTAC AATCAACCTA AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA 
     3661 CTATTCAGCA TCAGCAAGGA GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG 
     3721 ATGATGACCC CGAAGACCGC ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT 
     3781 GCGACAGCCG CCTCATCAGT GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG 
     3841 ACAATGAGCT GGATGAGCCA GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG 
     3901 AGCCGGTGCA GCACGAGGAG GCCAAAACCC AGAACAAAGA AGAATCCTAT GACTTCTCCA 
     3961 AATCCTATGA ATATAAGTCA AACCCCTCTG CCGTTGCTGG TAATGAAACT CCTGGGGCAT 
     4021 CTACCAAAGG TTATCCTCCT CCTGTTGCAG CAAAACCTAC CTTTGGGCGG TCTATACTGA 
     4081 AGCCCTCCAC TCCCATCCCT CCTCAAGAGG GTGAGGAGGT GGGAGAGAGC AGTGAGGAGC 
     4141 AAGATAATGC TCCCAAATCA GTCCTGGGCA AAGTCAAAAT ATTTGAGAAG ATGGATCACA 
     4201 AGGCCAGGTT ACAGAGAATG CAGGAGCTCC AGGAAGCACA GAATGCAAGG ATCGAAATTG 
     4261 CCCAGAAGCA TCCTGATATC TATGCAGTTC CAATCAAAAC GCACAAGCCA GACCCTGGCA 
     4321 CGCCCCAGCA CACGAGTTCC AGACCCCCTG AGCCACAGAA AGCTCCTTCC AGACCTTATC 
     4381 AGGATACCAG AGGAAGTTAT GGCAGTGATG CCGAGGAGGA GGAGTACCGC CAGCAGCTGT 
     4441 CAGAACACTC CAAGCGCGGT TACTATGGCC AGTCTGCCCG ATACCGGGAC ACAGAATTAA 
     4501 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4561 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4621 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4681 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4741 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4801 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4861 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4921 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4981 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5041 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5101 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5161 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5221 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5281 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5341 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5401 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5461 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5521 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5581 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5641 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5701 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5761 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5821 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5881 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5941 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6001 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6061 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6121 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6181 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6241 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6301 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6361 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6421 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6481 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6541 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6601 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6661 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6721 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6781 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6841 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6901 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6961 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7021 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7081 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7141 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7201 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7261 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7321 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7381 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7441 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7501 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7561 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7621 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7681 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7741 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7801 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7861 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7921 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7981 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8041 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8101 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8161 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8221 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8281 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8341 AACGCGAATT TT
//