LOCUS       RC230663                8334 bp    DNA     circular  SYN 
DEFINITION  PHKA1 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 3
ACCESSION   NM_001172436
VERSION     NM_001172436.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4491..4582 
                     /label="Myc-DDK"  
     misc_feature    4612..5197 
                     /label="PolyA signal"  
     misc_feature    4669..4688 
                     /label="Restriction Enzyme"  
     misc_feature    5473..6145 
                     /label="ColE1"  
     misc_feature    6575..7369 
                     /label="Kan/Neo"  
     misc_feature    7404..7706 
                     /label="SV40 ori"  
     misc_feature    7792..8269 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4473..4479 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4473 
                     /label="PHKA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAGCCGG AGTAACTCCG GGGTCCGGCT GGACGGCTAC GCTCGACTGG 
     1081 TGCAACAGAC CATCCTGTGC CATCAGAATC CAGTGACTGG CTTGCTTCCA GCCAGCTATG 
     1141 ATCAGAAAGA TGCTTGGGTC CGAGATAATG TGTACAGCAT CTTGGCTGTG TGGGGTTTGG 
     1201 GCCTGGCCTA TCGGAAGAAT GCAGACCGGG ATGAGGATAA GGCAAAGGCC TATGAATTGG 
     1261 AGCAGAGTGT AGTGAAGCTG ATGAGAGGAC TACTGCACTG CATGATCAGA CAGGTGGATA 
     1321 AAGTAGAATC CTTCAAATAT AGTCAGAGTA CTAAGGATAG CCTCCATGCA AAGTACAACA 
     1381 CCAAAACCTG TGCCACTGTA GTGGGTGATG ATCAATGGGG ACACCTGCAG TTGGATGCTA 
     1441 CCTCTGTGTA CCTGCTCTTC TTAGCCCAAA TGACTGCCTC AGGACTCCAT ATCATCCACA 
     1501 GCCTAGATGA AGTCAATTTC ATACAGAACC TTGTGTTTTA CATTGAAGCT GCATATAAAA 
     1561 CTGCTGACTT CGGGATATGG GAACGTGGAG ACAAGACCAA CCAAGGGATC TCAGAGTTGA 
     1621 ATGCCAGTTC AGTTGGAATG GCAAAGGCAG CCCTGGAAGC ATTAGATGAA CTGGATCTGT 
     1681 TTGGTGTGAA AGGTGGGCCT CAATCAGTTA TCCATGTCCT GGCTGATGAA GTACAGCACT 
     1741 GCCAGTCTAT CCTAAATTCA CTACTGCCCC GTGCTTCAAC ATCAAAAGAG GTTGATGCTA 
     1801 GTCTACTCTC AGTGGTTTCC TTCCCTGCCT TTGCAGTAGA GGATAGCCAG TTGGTGGAGC 
     1861 TCACAAAACA GGAAATCATC ACCAAGCTTC AGGGTCGTTA TGGTTGCTGT CGCTTTCTAC 
     1921 GAGATGGATA TAAAACTCCT AAAGAGGATC CCAATCGTCT GTACTATGAA CCAGCTGAGC 
     1981 TGAAGCTATT TGAAAACATT GAGTGTGAAT GGCCATTGTT CTGGACATAC TTTATTCTTG 
     2041 ATGGGGTCTT CAGTGGCAAT GCAGAACAGG TTCAAGAATA TAAAGAGGCT CTTGAAGCAG 
     2101 TCCTCATCAA GGGCAAAAAT GGAGTCCCAC TTCTGCCAGA GCTGTACAGT GTTCCTCCTG 
     2161 ACAGGGTCGA TGAAGAATAT CAGAATCCTC ACACTGTGGA CCGAGTCCCC ATGGGGAAAT 
     2221 TGCCTCACAT GTGGGGTCAG TCTCTATACA TTTTAGGAAG CTTGATGGCA GAGGGATTTT 
     2281 TAGCCCCTGG AGAAATTGAT CCCCTGAATC GCAGGTTTTC TACTGTACCG AAGCCCGATG 
     2341 TTGTGGTTCA AGTCTCCATT CTAGCTGAAA CAGAAGAAAT CAAGACCATT TTGAAGGACA 
     2401 AGGGAATTTA CGTGGAGACC ATTGCTGAGG TATACCCCAT CAGAGTACAA CCAGCTCGTA 
     2461 TTCTCAGCCA CATTTATTCC AGCCTAGGAT GCAACAATAG AATGAAACTC AGTGGACGAC 
     2521 CCTACAGACA CATGGGAGTG CTTGGAACTT CAAAACTCTA TGACATTCGG AAAACTATCT 
     2581 TTACTTTCAC TCCACAGTTT ATAGACCAGC AACAGTTCTA CCTGGCTCTG GACAACAAGA 
     2641 TGATAGTGGA AATGCTTAGA ACAGACCTCT CCTACCTCTG TAGCCGCTGG CGGATGACAG 
     2701 GCCAGCCCAC CATCACCTTC CCCATCTCAC ACAGCATGCT TGATGAAGAT GGAACAAGCT 
     2761 TGAATTCAAG TATCCTGGCA GCACTCCGAA AAATGCAAGA TGGGTATTTT GGTGGGGCAA 
     2821 GGGTTCAAAC AGGTAAATTG TCAGAGTTTT TGACAACATC TTGTTGCACA CACTTGAGCT 
     2881 TCATGGACCC TGGACCTGAG GGTAAGCTGT ACAGTGAAGA TTATGATGAC AACTATGATT 
     2941 ACCTGGAATC TGGCAACTGG ATGAATGATT ATGATTCAAC CAGTCATGAT GTTCACATGT 
     3001 ATCTTCCTAC GAAGTTATTT CAGGCTTCCC GGCCTTCATT CAACTTACTT GATTCACCTC 
     3061 ATCCCCGACA GGAGAACCAG GTTCCCTCTG TTCGTGTAGA AATACATCTT CCTAGAGACC 
     3121 AGTCTGGGGA GGTGGACTTT AAAGCACTGG TTTTACAGTT GAAGGAGACC TCAAGCTTAC 
     3181 AGGAACAAGC TGATATCCTC TATATGCTGT ATACTATGAA AGGACCTGAC TGGAACACTG 
     3241 AATTGTATAA TGAACGGAGT GCTACAGTGA GAGAGCTTCT TACCGAGCTG TATGGCAAAG 
     3301 TGGGAGAAAT TCGTCACTGG GGCCTGATCC GATACATTTC TGGGATCTTA AGGAAGAAAG 
     3361 TGGAAGCACT TGATGAGGCC TGCACAGACC TTCTCTCCCA CCAGAAACAT TTGACAGTAG 
     3421 GACTTCCTCC AGAACCTCGA GAAAAGACTA TCTCTGCACC TCTGCCCTAT GAGGCGCTCA 
     3481 CTCAGCTGAT AGATGAAGCC AGTGAAGGGG ATATGAGCAT TTCAATCCTT ACACAGGAAA 
     3541 TAATGGTATA TCTAGCCATG TATATGCGAA CCCAGCCTGG CCTCTTTGCT GAAATGTTTC 
     3601 GACTTCGAAT TGGTCTGATC ATACAAGTTA TGGCAACAGA ACTGGCCCAC TCCCTTCGAT 
     3661 GCTCAGCTGA GGAAGCCACA GAGGGCCTGA TGAATCTCAG TCCTTCGGCC ATGAAGAATC 
     3721 TCCTGCATCA CATTCTCAGC GGCAAGGAGT TTGGAGTGGA ACGAAGCGTT CGTCCCACTG 
     3781 ATTCAAATGT CAGTCCTGCT ATTTCTATCC ACGAGATTGG TGCTGTTGGA GCAACCAAAA 
     3841 CAGAACGAAC TGGGATCATG CAGTTAAAAA GTGAGATAAA GCAGTCACCT GGAACCTCTA 
     3901 TGACTCCAAG TAGTGGGTCC TTTCCTAGTG CATATGATCA GCAGTCATCT AAAGATAGTC 
     3961 GTCAAGGTCA ATGGCAACGC CGAAGAAGGC TGGATGGGGC ACTGAATAGA GTTCCAGTTG 
     4021 GATTTTATCA GAAAGTATGG AAAGTTTTGC AGAAGTGTCA CGGACTTTCT GTTGAAGGGT 
     4081 TTGTCCTTCC TTCCTCTACC ACTAGAGAGA TGACTCCAGG TGAGATTAAA TTCTCTGTTC 
     4141 ATGTGGAGTC TGTCCTGAAT CGTGTACCTC AGCCAGAGTA CCGTCAGCTG CTGGTTGAAG 
     4201 CCATCCTTGT CCTCACCATG CTGGCAGATA TTGAAATTCA TAGCATCGGA AGCATCATTG 
     4261 CTGTGGAAAA AATAGTGCAT ATTGCCAATG ACTTGTTCCT TCAAGAACAG AAAACCCTTG 
     4321 GCGCAGATGA TACCATGTTG GCAAAGGATC CCGCATCTGG CATCTGTACT CTTCTGTATG 
     4381 ACAGTGCACC CAGTGGCAGG TTTGGCACCA TGACCTACCT CTCCAAGGCA GCCGCCACCT 
     4441 ACGTGCAGGA GTTCCTGCCC CACAGCATCT GTGCCATGCA AACGCGTACG CGGCCGCTCG 
     4501 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4561 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4621 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4681 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4741 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4801 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4861 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4921 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4981 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5041 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5101 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5161 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5221 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5281 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5341 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5401 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5461 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5521 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5581 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5641 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5701 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5761 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5821 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5881 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5941 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6001 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6061 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6121 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6181 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6241 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6301 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6361 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6421 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6481 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6541 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6601 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6661 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6721 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6781 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6841 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6901 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6961 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7021 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7081 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7141 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7201 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7261 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7321 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7381 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7441 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7501 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7561 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7621 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7681 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7741 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7801 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7861 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7921 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7981 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8041 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8101 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8161 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8221 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8281 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//