LOCUS       RC230660                8319 bp    DNA     circular  SYN 
DEFINITION  PTCH2 (Myc-DDK-tagged)-Human patched 2 (PTCH2), transcript variant 2
ACCESSION   NM_001166292
VERSION     NM_001166292.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4476..4567 
                     /label="Myc-DDK"  
     misc_feature    4597..5182 
                     /label="PolyA signal"  
     misc_feature    4654..4673 
                     /label="Restriction Enzyme"  
     misc_feature    5458..6130 
                     /label="ColE1"  
     misc_feature    6560..7354 
                     /label="Kan/Neo"  
     misc_feature    7389..7691 
                     /label="SV40 ori"  
     misc_feature    7777..8254 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4458..4464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4458 
                     /label="PTCH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTCGATCG CCGCCCCTCA GAGAGCTGCC CCCGAGTTAC ACACCCCCAG 
     1081 CTCGAACCGC AGCACCCCAG ATCCTAGCTG GGAGCCTGAA GGCTCCACTC TGGCTTCGTG 
     1141 CTTACTTCCA GGGCCTGCTC TTCTCTCTGG GATGCGGGAT CCAGAGACAT TGTGGCAAAG 
     1201 TGCTCTTTCT GGGACTGTTG GCCTTTGGGG CCCTGGCATT AGGTCTCCGC ATGGCCATTA 
     1261 TTGAGACAAA CTTGGAACAG CTCTGGGTAG AAGTGGGCAG CCGGGTGAGC CAGGAGCTGC 
     1321 ATTACACCAA GGAGAAGCTG GGGGAGGAGG CTGCATACAC CTCTCAGATG CTGATACAGA 
     1381 CCGCACGCCA GGAGGGAGAG AACATCCTCA CACCCGAAGC ACTTGGCCTC CACCTCCAGG 
     1441 CAGCCCTCAC TGCCAGTAAA GTCCAAGTAT CACTCTATGG GAAGTCCTGG GATTTGAACA 
     1501 AAATCTGCTA CAAGTCAGGA GTTCCCCTTA TTGAAAATGG AATGATTGAG CGGATGATTG 
     1561 AGAAGCTGTT TCCGTGCGTG ATCCTCACCC CCCTCGACTG CTTCTGGGAG GGAGCCAAAC 
     1621 TCCAAGGGGG CTCCGCCTAC CTGCCCGGCC GCCCGGATAT CCAGTGGACC AACCTGGATC 
     1681 CAGAGCAGCT GCTGGAGGAG CTGGGTCCCT TTGCCTCCCT TGAGGGCTTC CGGGAGCTGC 
     1741 TAGACAAGGC ACAGGTGGGC CAGGCCTACG TGGGGCGGCC CTGTCTGCAC CCTGATGACC 
     1801 TCCACTGCCC ACCTAGTGCC CCCAACCATC ACAGCAGGCA GGCTCCCAAT GTGGCTCACG 
     1861 AGCTGAGTGG GGGCTGCCAT GGCTTCTCCC ACAAATTCAT GCACTGGCAG GAGGAATTGC 
     1921 TGCTGGGAGG CATGGCCAGA GACCCCCAAG GAGAGCTGCT GAGGGCAGAG GCCCTGCAGA 
     1981 GCACCTTCTT GCTGATGAGT CCCCGCCAGC TGTACGAGCA TTTCCGGGGT GACTATCAGA 
     2041 CACATGACAT TGGCTGGAGT GAGGAGCAGG CCAGCACAGT GCTACAAGCC TGGCAGCGGC 
     2101 GCTTTGTGCA GCTGGCCCAG GAGGCCCTGC CTGAGAACGC TTCCCAGCAG ATCCATGCCT 
     2161 TCTCCTCCAC CACCCTGGAT GACATCCTGC ATGCGTTCTC TGAAGTCAGT GCTGCCCGTG 
     2221 TGGTGGGAGG CTATCTGCTC ATGCTGGCCT ATGCCTGTGT GACCATGCTG CGGTGGGACT 
     2281 GCGCCCAGTC CCAGGGTTCC GTGGGCCTTG CCGGGGTACT GCTGGTGGCC CTGGCGGTGG 
     2341 CCTCAGGCCT TGGGCTCTGT GCCCTGCTCG GCATCACCTT CAATGCTGCC ACTACCCAGG 
     2401 TGCTGCCCTT CTTGGCTCTG GGAATCGGCG TGGATGACGT ATTCCTGCTG GCGCATGCCT 
     2461 TCACAGAGGC TCTGCCTGGC ACCCCTCTCC AGGAGCGCAT GGGCGAGTGT CTGCAGCGCA 
     2521 CGGGCACCAG TGTCGTACTC ACATCCATCA ACAACATGGC CGCCTTCCTC ATGGCTGCCC 
     2581 TCGTTCCCAT CCCTGCGCTG CGAGCCTTCT CCCTACAGGC GGCCATAGTG GTTGGCTGCA 
     2641 CCTTTGTAGC CGTGATGCTT GTCTTCCCAG CCATCCTCAG CCTGGACCTA CGGCGGCGCC 
     2701 ACTGCCAGCG CCTTGATGTG CTCTGCTGCT TCTCCAGTCC CTGCTCTGCT CAGGTGATTC 
     2761 AGATCCTGCC CCAGGAGCTG GGGGACGGGA CAGTACCAGT GGGCATTGCC CACCTCACTG 
     2821 CCACAGTTCA AGCCTTTACC CACTGTGAAG CCAGCAGCCA GCATGTGGTC ACCATCCTGC 
     2881 CTCCCCAAGC CCACCTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG CTCTTCAGCC 
     2941 CTGGAGGGTC CACACGGGAC CTTCTAGGCC AGGAGGAGGA GACAAGGCAG AAGGCAGCCT 
     3001 GCAAGTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT CGCCCGCTAT CAGTTTGCCC 
     3061 CGTTGCTGCT CCAGTCACAT GCTAAGGCCA TCGTGCTGGT GCTCTTTGGT GCTCTTCTGG 
     3121 GCCTGAGCCT CTACGGAGCC ACCTTGGTGC AAGACGGCCT GGCCCTGACG GATGTGGTGC 
     3181 CTCGGGGCAC CAAGGAGCAT GCCTTCCTGA GCGCCCAGCT CAGGTACTTC TCCCTGTACG 
     3241 AGGTGGCCCT GGTGACCCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC GCCCTCTTTG 
     3301 ATCTGCACCA GCGCTTCAGT TCCCTCAAGG CGGTGCTGCC CCCACCGGCC ACCCAGGCAC 
     3361 CCCGCACCTG GCTGCACTAT TACCGCAACT GGCTACAGGG AATCCAGGCT GCCTTTGACC 
     3421 AGGACTGGGC TTCTGGGCGC ATCACCCGCC ACTCGTACCG CAATGGCTCT GAGGATGGGG 
     3481 CCCTGGCCTA CAAGCTGCTC ATCCAGACTG GAGACGCCCA GGAGCCTCTG GATTTCAGCC 
     3541 AGCTGACCAC AAGGAAGCTG GTGGACAGAG AGGGACTGAT TCCACCCGAG CTCTTCTACA 
     3601 TGGGGCTGAC CGTGTGGGTG AGCAGTGACC CCCTGGGTCT GGCAGCCTCA CAGGCCAACT 
     3661 TCTACCCCCC ACCTCCTGAA TGGCTGCACG ACAAATACGA CACCACGGGG GAGAACCTTC 
     3721 GCATCCCGCC AGCTCAGCCC TTGGAGTTTG CCCAGTTCCC CTTCCTGCTG CGTGGCCTCC 
     3781 AGAAGACTGC AGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC GCAGAGGCCG 
     3841 GCCAGGCTGG GGTGCACGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG GAACAGTATC 
     3901 TGGGCCTGCG GCGCTGCTTC CTGCTGGCCG TCTGCATCCT GCTGGTGTGC ACTTTCCTCG 
     3961 TCTGTGCTCT GCTGCTCCTC AACCCCTGGA CGGCTGGCCT CATAGTGCTG GTCCTGGCGA 
     4021 TGATGACAGT GGAACTCTTT GGTATCATGG GTTTCCTGGG CATCAAGCTG AGTGCCATCC 
     4081 CCGTGGTGAT CCTTGTGGCC TCTGTAGGCA TTGGCGTTGA GTTCACAGTC CACGTGGCTC 
     4141 TGGGCTTCCT GACCACCCAG GGCAGCCGGA ACCTGCGGGC CGCCCATGCC CTTGAGCACA 
     4201 CATTTGCCCC CGTGACCGAT GGGGCCATCT CCACATTGCT GGGTCTGCTC ATGCTTGCTG 
     4261 GTTCCCACTT TGACTTCATT GTAAGGTACT TCTTTGCGGC GCTGACAGTG CTCACGCTCC 
     4321 TGGGCCTCCT CCATGGACTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG GGCCCGCCGC 
     4381 CAGAGGTGAT ACAGATGTAC AAGGAAAGCC CAGAGATCCT GAGTCCACCA GCTCCACAGG 
     4441 GAGGCGGGCT TAGGCCCGAG GAGATCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4501 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4561 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4621 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4681 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4741 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4801 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4861 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4921 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4981 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5041 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5101 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5161 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5221 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5281 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5341 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5401 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5461 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5521 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5581 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5641 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5701 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5761 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5821 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5881 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5941 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6001 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6061 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6121 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6181 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6241 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6301 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6361 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6421 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6481 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6541 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6601 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6661 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6721 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6781 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6841 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6901 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6961 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7021 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7081 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7141 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7201 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7261 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7321 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7381 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7441 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7501 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7561 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7741 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7801 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7861 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7921 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7981 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8041 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8101 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8161 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8221 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8281 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//