LOCUS       RC230659                8316 bp    DNA     circular  SYN 
DEFINITION  SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5
ACCESSION   NM_001174061
VERSION     NM_001174061.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4473..4564 
                     /label="Myc-DDK"  
     misc_feature    4594..5179 
                     /label="PolyA signal"  
     misc_feature    4651..4670 
                     /label="Restriction Enzyme"  
     misc_feature    5455..6127 
                     /label="ColE1"  
     misc_feature    6557..7351 
                     /label="Kan/Neo"  
     misc_feature    7386..7688 
                     /label="SV40 ori"  
     misc_feature    7774..8251 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4455..4461 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4455 
                     /label="SMG7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTAGTTACC GATTTGGAAT ACGCTTTAGA CAAGAAAGTA GAACAGGATC 
     1081 TCTGGAATCA CGCCTTTAAG AATCAGATCA CAACACTGCA AGGCCAGGCA AAGAATCGAG 
     1141 CAAATCCGAA TCGGAGTGAA GTTCAGGCAA ACCTTTCTCT GTTCCTAGAG GCAGCTAGTG 
     1201 GCTTCTATAC TCAGTTATTA CAAGAACTGT GTACAGTATT TAATGTAGAT TTACCATGCC 
     1261 GTGTGAAGTC TTCCCAATTG GGAATTATCA GCAATAAACA GACGCATACC AGCGCCATAG 
     1321 TGAAGCCACA GTCTAGCTCC TGTTCCTATA TCTGCCAGCA CTGCCTCGTC CACCTTGGAG 
     1381 ACATTGCTCG ATACAGAAAC CAGACCAGCC AGGCAGAGTC CTACTATAGG CATGCAGCTC 
     1441 AGCTTGTCCC CTCCAATGGT CAGCCTTATA ATCAGTTGGC TATCTTAGCT TCTTCCAAAG 
     1501 GAGACCATCT GACCACAATT TTCTACTACT GCAGAAGCAT TGCTGTGAAG TTCCCTTTCC 
     1561 CAGCTGCCTC CACTAATCTG CAAAAAGCAC TTTCTAAAGC ACTGGAAAGC CGAGATGAGG 
     1621 TGAAAACCAA GTGGGGTGTT TCTGACTTCA TCAAGGCCTT TATTAAATTC CACGGTCATG 
     1681 TGTACCTGAG TAAGAGCTTG GAAAAGTTGA GCCCTCTTCG AGAGAAATTG GAAGAACAGT 
     1741 TTAAGAGGCT GCTATTCCAA AAAGCTTTCA ACTCTCAGCA GTTAGTTCAT GTCACTGTCA 
     1801 TTAACCTGTT TCAACTTCAT CACCTTCGTG ACTTTAGCAA TGAAACCGAG CAGCACACTT 
     1861 ATAGCCAAGA TGAGCAGCTA TGTTGGACAC AGTTGCTGGC CCTCTTTATG TCTTTTCTTG 
     1921 GCATCCTGTG CAAGTGTCCT CTACAGAATG AGTCTCAGGA GGAGTCCTAC AATGCCTATC 
     1981 CTCTTCCAGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CAGACCCAGG GTCTTTCAGG 
     2041 AGGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGTT GATTTCTCTT CTGAATAGTT 
     2101 TCCATCCCCA TGAAGAGGAC CTCTCAAGTA TTAGTGCGAC ACCACTTCCA GAGGAGTTTG 
     2161 AATTACAAGG ATTTTTGGCA TTGAGACCTT CTTTCAGGAA CTTGGATTTT TCCAAAGGTC 
     2221 ACCAGGGTAT TACAGGGGAC AAAGAAGGCC AGCAACGACG AATACGACAG CAACGCTTGA 
     2281 TCTCTATAGG CAAATGGATT GCTGATAATC AGCCAAGGCT GATTCAGTGT GAAAATGAGG 
     2341 TAGGGAAATT GTTGTTTATC ACAGAAATCC CAGAATTAAT ACTGGAAGAC CCCAGTGAAG 
     2401 CCAAAGAGAA CCTCATTCTG CAAGAAACAT CTGTGATAGA GTCGCTGGCT GCAGATGGGA 
     2461 GCCCAGGGCT AAAATCAGTG CTATCTACAA GCCGAAATTT AAGCAACAAC TGTGACACAG 
     2521 GAGAGAAGCC AGTGGTTACC TTCAAAGAAA ACATTAAGAC ACGAGAAGTG AACAGAGACC 
     2581 AAGGAAGAAG TTTTCCTCCC AAAGAGGTGA GAAGGGACTA TAGCAAAGGA ATAACTGTAA 
     2641 CTAAGAATGA TGGAAAGAAG GACAACAACA AGAGGAAAAC TGAAACCAAG AAATGCACCT 
     2701 TAGAAAAGTT ACAGGAAACA GGAAAGCAGA ATGTGGCAGT GCAGGTAAAA TCCCAGACAG 
     2761 AACTAAGAAA GACTCCAGTG TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC 
     2821 AAGCAAGTAA CTCCCAGTTC ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA 
     2881 GCAGGCCAGG GTTTCCGCCC CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG 
     2941 GCTCAGGTTA CACCTTCCCA GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG 
     3001 CTCACTCTCC AGCAGGAAAC CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC 
     3061 AGCAACGGCC CTCTGGACCA GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT 
     3121 CCCAGCAACC CCTTACATCT TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC 
     3181 AGGTTCAAGC TCTAACTCAG CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA 
     3241 AAAGCCCGCC TCACCACTCT GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC 
     3301 TGTGGAATCC CCCTCAGGTT CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT 
     3361 ACTACCTTCA GACCCAAGAC CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA 
     3421 TGCAGCAGCA GCCTCTAGAA AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA 
     3481 ATCCCTCAGA AGTCAAGGTC CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA 
     3541 ACAGATCTGT AATGGCACAG CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG 
     3601 TCTTCCGTCC AGAGCAGGAT CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC 
     3661 CTCTGCTTCC TCCGGACCTG TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA 
     3721 TTTTTTCTAA CCCTCCTGAT CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG 
     3781 GACGAAGCCT TTTTAAATCC TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT 
     3841 CTTTCCTGTC CCTCACCGGA TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT 
     3901 TCAATGAGGT ATATGGGAAA AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA 
     3961 TGGCCCCCCA GGAAACATCT CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC 
     4021 TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA 
     4081 GCAGCCTACC CAGCTCTCCT CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG 
     4141 GACCCATTGG GACTCCAGAT AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA 
     4201 AGCCAGCCAT GGGTGGGTTT GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA 
     4261 GTTGGCATCA GGCCAGCACT CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG 
     4321 ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC 
     4381 CTGGACCTTC TGCTCTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAACGGGGAC 
     4441 AAGGCACCAT GAACCCTCCA CACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4501 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4561 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4621 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4681 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4741 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4801 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4861 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4921 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4981 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5041 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5101 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5161 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5221 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5281 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5341 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5401 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5461 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5521 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5581 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5641 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5701 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5761 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5821 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5881 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5941 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6001 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6061 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6121 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6181 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6241 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6301 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6361 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6421 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6481 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6541 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6601 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6661 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6721 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6781 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6841 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6901 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6961 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7021 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7081 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7141 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7201 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7261 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7321 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7381 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7441 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7501 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7561 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7681 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7741 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7801 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7861 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7921 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7981 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8041 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8101 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8161 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8221 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8281 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//