LOCUS       RC230656                8256 bp    DNA     circular  SYN 
DEFINITION  ZEB1 (Myc-DDK-tagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 9
ACCESSION   NM_001174096
VERSION     NM_001174096.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4413..4504 
                     /label="Myc-DDK"  
     misc_feature    4534..5119 
                     /label="PolyA signal"  
     misc_feature    4591..4610 
                     /label="Restriction Enzyme"  
     misc_feature    5395..6067 
                     /label="ColE1"  
     misc_feature    6497..7291 
                     /label="Kan/Neo"  
     misc_feature    7326..7628 
                     /label="SV40 ori"  
     misc_feature    7714..8191 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4395..4401 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4395 
                     /label="ZEB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGATGGC CCCAGGTGTA AGCGCAGAAA GCAGGCGAAC CCGCGGCGCA 
     1081 ATAACGTTAC AAATTATAAT ACTGTGGTAG AAACAAATTC AGATTCAGAT GATGAAGACA 
     1141 AACTGCATAT TGTGGAAGAA GAAAGTGTTA CAGATGCAGC TGACTGTGAA GGTGTACCAG 
     1201 AGGATGACCT GCCAACAGAC CAGACAGTGT TACCAGGGAG GAGCAGTGAA AGAGAAGGGA 
     1261 ATGCTAAGAA CTGCTGGGAG GATGACACAG GAAAGGAAGG GCAAGAAATC CTGGGGCCTG 
     1321 AAGCTCAGGC AGATGAAGCA GGATGTACAG TAAAAGATGA TGAATGCGAG TCAGATGCAG 
     1381 AAAATGAGCA AAACCATGAT CCTAATGTTG AAGAGTTTCT ACAACAACAA GACACTGCTG 
     1441 TCATTTTTCC TGAGGCACCT GAAGAGGACC AGAGGCAGGG CACACCAGAA GCCAGTGGTC 
     1501 ATGATGAAAA TGGAACACCA GATGCATTTT CACAATTACT CACCTGTCCA TATTGTGATA 
     1561 GAGGCTATAA ACGCTTTACC TCTCTGAAAG AACACATTAA ATATCGTCAT GAAAAGAATG 
     1621 AAGATAACTT TAGTTGCTCC CTGTGCAGTT ACACCTTTGC ATACAGAACC CAACTTGAAC 
     1681 GTCACATGAC ATCACATAAA TCAGGAAGAG ATCAAAGACA TGTGACGCAG TCTGGGTGTA 
     1741 ATCGTAAATT CAAATGCACT GAGTGTGGAA AAGCTTTCAA ATACAAACAT CACCTAAAAG 
     1801 AGCACTTAAG AATTCACAGT GGAGAGAAGC CATATGAATG CCCAAACTGC AAGAAACGCT 
     1861 TTTCCCATTC TGGCTCCTAT AGCTCACACA TAAGCAGTAA GAAATGTATC AGCTTGATAC 
     1921 CTGTGAATGG GCGACCAAGA ACAGGACTCA AGACATCTCA GTGTTCTTCA CCGTCTCTTT 
     1981 CAGCATCACC AGGCAGTCCC ACACGACCAC AGATACGGCA AAAGATAGAG AATAAACCCC 
     2041 TTCAAGAACA ACTTTCTGTT AACCAAATTA AAACTGAACC TGTGGATTAT GAATTCAAAC 
     2101 CCATAGTGGT TGCTTCAGGA ATCAACTGTT CAACCCCTTT ACAAAATGGG GTTTTCACTG 
     2161 GTGGTGGCCC ATTACAGGCA ACCAGTTCTC CTCAGGGCAT GGTGCAAGCT GTTGTTCTGC 
     2221 CAACAGTTGG TTTGGTGTCT CCCATAAGTA TCAATTTAAG TGATATTCAG AATGTACTTA 
     2281 AAGTGGCGGT AGATGGTAAT GTAATAAGGC AAGTGTTGGA GAATAATCAA GCCAATCTTG 
     2341 CATCCAAAGA ACAAGAAACA ATCAATGCTT CACCCATACA ACAAGGTGGC CATTCTGTTA 
     2401 TTTCAGCCAT CAGTCTTCCT TTGGTTGATC AAGATGGAAC AACCAAAATT ATCATCAACT 
     2461 ACAGTCTTGA GCAGCCTAGC CAACTTCAAG TTGTTCCTCA AAATTTAAAA AAAGAAAATC 
     2521 CAGTCGCTAC AAACAGTTGT AAAAGTGAAA AGTTACCAGA AGATCTTACT GTTAAGTCTG 
     2581 AGAAGGACAA AAGCTTTGAA GGGGGGGTGA ATGATAGCAC TTGTCTTCTG TGTGATGATT 
     2641 GTCCAGGAGA TATTAATGCA CTTCCAGAAT TAAAGCACTA TGACCTAAAG CAGCCTACTC 
     2701 AGCCTCCTCC ACTCCCTGCA GCAGAAGCTG AGAAGCCTGA GTCCTCTGTT TCATCAGCTA 
     2761 CTGGAGATGG CAATTTGTCT CCTAGTCAGC CACCTTTAAA GAACCTCTTG TCTCTCCTAA 
     2821 AAGCATATTA TGCTTTGAAT GCACAACCAA GTGCAGAAGA GCTCTCAAAA ATTGCTGATT 
     2881 CAGTAAACCT ACCACTGGAT GTAGTAAAAA AGTGGTTTGA AAAGATGCAA GCTGGACAGA 
     2941 TTTCAGTGCA GTCTTCTGAA CCATCTTCTC CTGAACCAGG CAAAGTAAAT ATCCCTGCCA 
     3001 AGAACAATGA TCAGCCTCAA TCTGCAAATG CAAATGAACC CCAGGACAGC ACAGTAAATC 
     3061 TACAAAGTCC TTTGAAGATG ACTAACTCCC CAGTTTTACC AGTGGGATCA ACCACCAATG 
     3121 GTTCCAGAAG TAGTACACCA TCCCCATCAC CTCTAAACCT TTCCTCATCC AGAAATACAC 
     3181 AGGGTTACTT GTACACAGCT GAGGGTGCAC AAGAAGAGCC ACAAGTAGAA CCTCTTGATC 
     3241 TTTCACTACC AAAGCAACAG GGAGAATTAT TAGAAAGGTC AACTATCACT AGTGTTTACC 
     3301 AGAACAGTGT TTATTCTGTC CAGGAAGAAC CCTTGAACTT GTCTTGCGCA AAAAAGGAGC 
     3361 CACAAAAGGA CAGTTGTGTT ACAGACTCAG AACCAGTTGT AAATGTAATC CCACCAAGTG 
     3421 CCAACCCCAT AAATATCGCT ATACCTACAG TCACTGCCCA GTTACCCACA ATCGTGGCCA 
     3481 TTGCTGACCA GAACAGTGTT CCATGCTTAA GAGCGCTAGC TGCCAATAAG CAAACGATTC 
     3541 TGATTCCCCA GGTGGCATAC ACCTACTCAA CTACGGTCAG CCCTGCAGTC CAAGAACCAC 
     3601 CCTTGAAAGT GATCCAGCCA AATGGAAATC AGGATGAAAG ACAAGATACT AGCTCAGAAG 
     3661 GAGTATCAAA TGTAGAGGAT CAGAATGACT CTGATTCTAC ACCGCCCAAA AAGAAAATGC 
     3721 GGAAGACAGA AAATGGAATG TATGCTTGTG ATTTGTGTGA CAAGATATTC CAAAAGAGTA 
     3781 GTTCATTATT GAGACATAAA TATGAACACA CAGGTAAAAG ACCTCATGAG TGTGGAATCT 
     3841 GTAAAAAGGC ATTTAAACAC AAACATCATT TGATTGAACA CATGCGATTA CATTCTGGAG 
     3901 AAAAGCCCTA TCAATGTGAC AAATGTGGAA AGCGCTTCTC ACACTCTGGG TCTTATTCTC 
     3961 AACACATGAA TCATCGCTAC TCCTACTGTA AGAGAGAAGC GGAAGAACGT GACAGCACAG 
     4021 AGCAGGAAGA GGCAGGGCCT GAAATCCTCT CGAATGAGCA CGTGGGTGCC AGGGCGTCTC 
     4081 CCTCACAGGG CGACTCGGAC GAGAGAGAGA GTTTGACAAG GGAAGAGGAT GAAGACAGTG 
     4141 AAAAAGAGGA AGAGGAGGAG GATAAAGAGA TGGAAGAATT GCAGGAAGAA AAAGAATGTG 
     4201 AAAAACCACA AGGGGATGAG GAAGAGGAGG AGGAGGAGGA AGAAGTGGAA GAAGAAGAGG 
     4261 TAGAAGAGGC AGAGAATGAG GGAGAAGAAG CAAAAACTGA AGGTCTGATG AAGGATGACA 
     4321 GGGCTGAAAG TCAAGCAAGC AGCTTAGGAC AAAAAGTAGG CGAGAGTAGT GAGCAAGTGT 
     4381 CTGAAGAAAA GACAAATGAA GCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4441 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4501 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4561 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4621 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4681 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4741 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4801 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4861 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4921 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4981 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5041 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5101 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5161 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5221 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5281 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5341 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5401 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5461 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5521 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5581 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5641 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5701 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5761 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5821 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5881 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5941 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6001 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6061 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6121 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6181 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6241 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6301 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6361 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6421 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6481 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6541 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6601 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6661 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6721 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6781 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6841 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6901 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6961 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7021 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7081 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7141 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7201 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7261 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7321 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7381 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7441 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7501 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7621 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7681 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7741 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7801 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7861 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7921 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7981 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8041 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8101 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8161 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8221 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//