LOCUS       RC230653                8223 bp    DNA     circular  SYN 
DEFINITION  ESYT1 (Myc-DDK-tagged)-Human extended synaptotagmin-like protein 1 (ESYT1), transcript variant 1
ACCESSION   NM_001184796
VERSION     NM_001184796.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4380..4471 
                     /label="Myc-DDK"  
     misc_feature    4501..5086 
                     /label="PolyA signal"  
     misc_feature    4558..4577 
                     /label="Restriction Enzyme"  
     misc_feature    5362..6034 
                     /label="ColE1"  
     misc_feature    6464..7258 
                     /label="Kan/Neo"  
     misc_feature    7293..7595 
                     /label="SV40 ori"  
     misc_feature    7681..8158 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4362..4368 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4362 
                     /label="ESYT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGATCT CCAGGAGAGG GCCCCAGCCC CAGCCCCATG GACCAGCCCT 
     1081 CTGCTCCCTC CGACCCCACT GACCAGCCCC CCGCTGCTCA CGCAAAGCCA GACCCAGGTT 
     1141 CTGGGGGCCA ACCTGCTGGC CCTGGCGCGG CGGGTGAGGC CCTGGCGGTG CTGACTTCAT 
     1201 TCGGGAGGCG GTTGCTGGTG CTGATACCTG TGTATTTGGC CGGGGCAGTG GGACTCAGCG 
     1261 TGGGTTTCGT GCTCTTCGGC CTCGCCCTCT ACCTGGGCTG GCGCCGGGTC CGCGACGAGA 
     1321 AAGAACGGAG CCTTCGAGCA GCGAGGCAGC TACTGGACGA CGAGGAGCAG CTCACTGCGA 
     1381 AAACTCTCTA TATGAGTCAT CGAGAGCTAC CTGCCTGGGT CAGCTTCCCA GACGTGGAAA 
     1441 AGGCTGAATG GCTCAATAAG ATTGTGGCCC AGGTCTGGCC CTTCCTGGGC CAGTATATGG 
     1501 AGAAGCTTCT GGCTGAAACT GTGGCTCCGG CTGTTAGGGG ATCTAACCCC CATCTGCAAA 
     1561 CATTTACATT TACACGAGTG GAACTGGGTG AAAAGCCATT GCGCATCATT GGAGTCAAGG 
     1621 TTCACCCAGG TCAGAGAAAA GAGCAGATCC TGCTGGACTT GAACATCAGC TATGTAGGTG 
     1681 ATGTGCAGAT TGATGTGGAA GTGAAGAAAT ATTTTTGCAA AGCAGGAGTC AAGGGCATGC 
     1741 AGCTACATGG CGTTTTGCGG GTGATACTGG AGCCACTCAT TGGGGACCTT CCCTTCGTGG 
     1801 GGGCTGTGTC AATGTTCTTC ATCCGACGCC CGACCCTAGA CATCAACTGG ACAGGGATGA 
     1861 CCAACCTGCT GGATATCCCA GGACTTAGCT CACTCTCTGA CACCATGATC ATGGACTCCA 
     1921 TTGCTGCCTT CCTCGTGTTG CCCAACCGAT TACTGGTGCC CCTTGTGCCT GACCTTCAAG 
     1981 ATGTGGCTCA GTTGCGTTCC CCTCTGCCCA GGGGCATTAT TCGAATTCAC CTGCTGGCTG 
     2041 CTCGAGGGCT GAGTTCCAAG GACAAATATG TGAAGGGCCT GATTGAGGGC AAGTCAGACC 
     2101 CATATGCACT TGTGCGTTTG GGTACCCAGA CATTCTGCAG TCGTGTCATT GATGAAGAAC 
     2161 TCAACCCACA GTGGGGAGAG ACTTATGAGG TGATGGTACA CGAGGTCCCA GGGCAGGAGA 
     2221 TTGAAGTGGA GGTGTTCGAC AAGGATCCAG ATAAAGATGA CTTTCTGGGC AGAATGAAGC 
     2281 TGGATGTAGG GAAGGTGTTA CAGGCTAGCG TTCTGGATGA TTGGTTCCCT CTACAAGGTG 
     2341 GGCAAGGCCA AGTTCACTTG AGGCTAGAAT GGCTGTCACT TTTGTCAGAT GCAGAGAAAC 
     2401 TGGAGCAGGT TCTACAGTGG AATTGGGGAG TCTCCTCTCG ACCAGATCCC CCGTCAGCTG 
     2461 CCATCTTAGT TGTCTACCTG GATCGGGCCC AGGATCTTCC TATGGTGACC TCTGAATTGT 
     2521 ACCCACCACA GCTGAAGAAG GGGAACAAGG AACCCAACCC TATGGTACAA CTGTCAATTC 
     2581 AGGATGTGAC TCAGGAGAGC AAGGCTGTCT ACAGTACCAA CTGCCCAGTG TGGGAGGAAG 
     2641 CGTTCCGGTT CTTCCTACAA GACCCTCAAA GCCAGGAGCT CGATGTGCAA GTGAAGGATG 
     2701 ATTCCAGGGC CCTGACTTTA GGAGCACTGA CGCTGCCTCT GGCCCGCCTG CTGACTGCCC 
     2761 CAGAACTCAT CCTGGACCAG TGGTTCCAGC TCAGCAGCTC TGGTCCAAAC TCCAGACTCT 
     2821 ATATGAAACT AGTCATGAGG ATCCTGTACT TGGATTCATC AGAAATATGC TTCCCCACGG 
     2881 TGCCTGGTTG TCCTGGTGCT TGGGACGTGG ACAGTGAGAA TCCCCAGAGA GGCAGCAGTG 
     2941 TGGATGCCCC ACCTCGACCC TGTCACACGA CTCCTGATAG CCAGTTTGGG ACTGAGCATG 
     3001 TGCTTCGGAT CCATGTATTA GAGGCCCAGG ACCTGATTGC CAAAGACCGT TTCTTGGGGG 
     3061 GACTGGTGAA GGGCAAGTCA GACCCCTATG TCAAACTAAA GTTGGCAGGA CGAAGCTTCC 
     3121 GGAGCCATGT TGTTCGGGAA GATCTCAATC CCCGCTGGAA TGAGGTTTTT GAGGTGATCG 
     3181 TCACATCAGT TCCAGGCCAA GAGCTAGAGG TTGAAGTCTT TGACAAGGAC TTGGACAAGG 
     3241 ATGATTTTCT GGGCAGGTGT AAAGTGCGTC TCACCACAGT CTTAAACAGT GGCTTCCTTG 
     3301 ATGAGTGGCT GACCCTGGAG GATGTCCCAT CTGGCCGCCT GCACTTGCGC CTGGAGCGTC 
     3361 TCACCCCCCG TCCCACTGCT GCTGAGTTAG AGGAGGTGCT GCAGGTGAAT AGTTTGATCC 
     3421 AGACTCAGAA GAGTGCGGAG CTGGCTGCGG CCCTGCTATC CATCTATATG GAGCGGGCAG 
     3481 AGGACCTCCC GCTGCGAAAA GGCACCAAGC ACCTCAGCCC TTATGCTACT CTCACTGTGG 
     3541 GAGATAGTTC TCATAAAACC AAGACTATTT CGCAAACTTC AGCCCCTGTC TGGGATGAGA 
     3601 GTGCCTCCTT TCTCATCAGG AAACCACACA CTGAGAGCCT AGAGTTGCAG GTTCGGGGTG 
     3661 AGGGCACTGG CGTGCTGGGC TCATTATCCC TGCCCCTCTC AGAGCTCCTC GTGGCTGACC 
     3721 AGCTCTGCTT GGACCGCTGG TTTACACTCA GCAGTGGTCA GGGGCAGGTG CTACTGAGAG 
     3781 CACAGCTAGG GATCCTGGTG TCCCAGCACT CGGGAGTGGA AGCTCATAGC CACAGCTACA 
     3841 GCCACAGCTC CTCATCGCTG AGTGAAGAAC CAGAGCTCTC GGGGGGACCC CCTCACATCA 
     3901 CCTCCTCAGC CCCAGAGCTC CGGCAGCGCC TAACACATGT TGACAGTCCC CTTGAGGCTC 
     3961 CAGCCGGGCC TCTGGGCCAG GTGAAACTGA CTCTGTGGTA CTACAGTGAA GAACGAAAGC 
     4021 TGGTCAGCAT TGTTCATGGT TGCCGGTCCC TTCGACAGAA TGGACGTGAT CCTCCTGATC 
     4081 CCTATGTGTC ACTGTTGCTA CTGCCAGACA AGAACCGAGG CACCAAGAGG AGGACCTCAC 
     4141 AGAAGAAGAG GACCCTGAGT CCTGAATTTA ATGAACGGTT TGAGTGGGAA CTCCCCCTGG 
     4201 ATGAGGCCCA GAGACGAAAG CTGGATGTCT CTGTCAAGTC TAATTCCTCC TTCATGTCAA 
     4261 GAGAGCGTGA GCTGCTGGGG AAGGTGCAGC TGGACCTAGC TGAGACAGAC CTTTCCCAGG 
     4321 GTGTAGCCCG GTGGTATGAC CTGATGGACA ACAAGGACAA GGGCAGCTCC ACGCGTACGC 
     4381 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4441 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4501 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4561 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4621 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4681 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4741 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4801 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4861 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4921 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4981 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5041 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5101 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5161 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5221 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5281 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5341 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5401 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5461 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5521 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5581 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5641 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5701 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5761 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5821 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5881 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5941 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6001 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6061 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6121 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6181 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6241 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6301 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6361 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6421 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6481 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6541 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6601 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6661 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6721 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6781 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6841 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6901 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6961 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7021 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7081 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7141 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7201 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7261 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7321 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7381 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7441 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7501 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7561 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7621 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7681 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7741 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7801 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7861 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7921 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7981 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8041 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8101 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8161 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8221 TTT
//