LOCUS       RC230651                8202 bp    DNA     circular  SYN 
DEFINITION  ZEB1 (Myc-DDK-tagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 7
ACCESSION   NM_001174094
VERSION     NM_001174094.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4359..4450 
                     /label="Myc-DDK"  
     misc_feature    4480..5065 
                     /label="PolyA signal"  
     misc_feature    4537..4556 
                     /label="Restriction Enzyme"  
     misc_feature    5341..6013 
                     /label="ColE1"  
     misc_feature    6443..7237 
                     /label="Kan/Neo"  
     misc_feature    7272..7574 
                     /label="SV40 ori"  
     misc_feature    7660..8137 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4341..4347 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4341 
                     /label="ZEB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTTACA AATTATAATA CTGTGGTAGA AACAAATTCA GATTCAGATG 
     1081 ATGAAGACAA ACTGCATATT GTGGAAGAAG AAAGTGTTAC AGATGCAGCT GACTGTGAAG 
     1141 GTGTACCAGA GGATGACCTG CCAACAGACC AGACAGTGTT ACCAGGGAGG AGCAGTGAAA 
     1201 GAGAAGGGAA TGCTAAGAAC TGCTGGGAGG ATGACAGAAA GGAAGGGCAA GAAATCCTGG 
     1261 GGCCTGAAGC TCAGGCAGAT GAAGCAGGAT GTACAGTAAA AGATGATGAA TGCGAGTCAG 
     1321 ATGCAGAAAA TGAGCAAAAC CATGATCCTA ATGTTGAAGA GTTTCTACAA CAACAAGACA 
     1381 CTGCTGTCAT TTTTCCTGAG GCACCTGAAG AGGACCAGAG GCAGGGCACA CCAGAAGCCA 
     1441 GTGGTCATGA TGAAAATGGA ACACCAGATG CATTTTCACA ATTACTCACC TGTCCATATT 
     1501 GTGATAGAGG CTATAAACGC TTTACCTCTC TGAAAGAACA CATTAAATAT CGTCATGAAA 
     1561 AGAATGAAGA TAACTTTAGT TGCTCCCTGT GCAGTTACAC CTTTGCATAC AGAACCCAAC 
     1621 TTGAACGTCA CATGACATCA CATAAATCAG GAAGAGATCA AAGACATGTG ACGCAGTCTG 
     1681 GGTGTAATCG TAAATTCAAA TGCACTGAGT GTGGAAAAGC TTTCAAATAC AAACATCACC 
     1741 TAAAAGAGCA CTTAAGAATT CACAGTGGAG AGAAGCCATA TGAATGCCCA AACTGCAAGA 
     1801 AACGCTTTTC CCATTCTGGC TCCTATAGCT CACACATAAG CAGTAAGAAA TGTATCAGCT 
     1861 TGATACCTGT GAATGGGCGA CCAAGAACAG GACTCAAGAC ATCTCAGTGT TCTTCACCGT 
     1921 CTCTTTCAGC ATCACCAGGC AGTCCCACAC GACCACAGAT ACGGCAAAAG ATAGAGAATA 
     1981 AACCCCTTCA AGAACAACTT TCTGTTAACC AAATTAAAAC TGAACCTGTG GATTATGAAT 
     2041 TCAAACCCAT AGTGGTTGCT TCAGGAATCA ACTGTTCAAC CCCTTTACAA AATGGGGTTT 
     2101 TCACTGGTGG TGGCCCATTA CAGGCAACCA GTTCTCCTCA GGGCATGGTG CAAGCTGTTG 
     2161 TTCTGCCAAC AGTTGGTTTG GTGTCTCCCA TAAGTATCAA TTTAAGTGAT ATTCAGAATG 
     2221 TACTTAAAGT GGCGGTAGAT GGTAATGTAA TAAGGCAAGT GTTGGAGAAT AATCAAGCCA 
     2281 ATCTTGCATC CAAAGAACAA GAAACAATCA ATGCTTCACC CATACAACAA GGTGGCCATT 
     2341 CTGTTATTTC AGCCATCAGT CTTCCTTTGG TTGATCAAGA TGGAACAACC AAAATTATCA 
     2401 TCAACTACAG TCTTGAGCAG CCTAGCCAAC TTCAAGTTGT TCCTCAAAAT TTAAAAAAAG 
     2461 AAAATCCAGT CGCTACAAAC AGTTGTAAAA GTGAAAAGTT ACCAGAAGAT CTTACTGTTA 
     2521 AGTCTGAGAA GGACAAAAGC TTTGAAGGGG GGGTGAATGA TAGCACTTGT CTTCTGTGTG 
     2581 ATGATTGTCC AGGAGATATT AATGCACTTC CAGAATTAAA GCACTATGAC CTAAAGCAGC 
     2641 CTACTCAGCC TCCTCCACTC CCTGCAGCAG AAGCTGAGAA GCCTGAGTCC TCTGTTTCAT 
     2701 CAGCTACTGG AGATGGCAAT TTGTCTCCTA GTCAGCCACC TTTAAAGAAC CTCTTGTCTC 
     2761 TCCTAAAAGC ATATTATGCT TTGAATGCAC AACCAAGTGC AGAAGAGCTC TCAAAAATTG 
     2821 CTGATTCAGT AAACCTACCA CTGGATGTAG TAAAAAAGTG GTTTGAAAAG ATGCAAGCTG 
     2881 GACAGATTTC AGTGCAGTCT TCTGAACCAT CTTCTCCTGA ACCAGGCAAA GTAAATATCC 
     2941 CTGCCAAGAA CAATGATCAG CCTCAATCTG CAAATGCAAA TGAACCCCAG GACAGCACAG 
     3001 TAAATCTACA AAGTCCTTTG AAGATGACTA ACTCCCCAGT TTTACCAGTG GGATCAACCA 
     3061 CCAATGGTTC CAGAAGTAGT ACACCATCCC CATCACCTCT AAACCTTTCC TCATCCAGAA 
     3121 ATACACAGGG TTACTTGTAC ACAGCTGAGG GTGCACAAGA AGAGCCACAA GTAGAACCTC 
     3181 TTGATCTTTC ACTACCAAAG CAACAGGGAG AATTATTAGA AAGGTCAACT ATCACTAGTG 
     3241 TTTACCAGAA CAGTGTTTAT TCTGTCCAGG AAGAACCCTT GAACTTGTCT TGCGCAAAAA 
     3301 AGGAGCCACA AAAGGACAGT TGTGTTACAG ACTCAGAACC AGTTGTAAAT GTAATCCCAC 
     3361 CAAGTGCCAA CCCCATAAAT ATCGCTATAC CTACAGTCAC TGCCCAGTTA CCCACAATCG 
     3421 TGGCCATTGC TGACCAGAAC AGTGTTCCAT GCTTAAGAGC GCTAGCTGCC AATAAGCAAA 
     3481 CGATTCTGAT TCCCCAGGTG GCATACACCT ACTCAACTAC GGTCAGCCCT GCAGTCCAAG 
     3541 AACCACCCTT GAAAGTGATC CAGCCAAATG GAAATCAGGA TGAAAGACAA GATACTAGCT 
     3601 CAGAAGGAGT ATCAAATGTA GAGGATCAGA ATGACTCTGA TTCTACACCG CCCAAAAAGA 
     3661 AAATGCGGAA GACAGAAAAT GGAATGTATG CTTGTGATTT GTGTGACAAG ATATTCCAAA 
     3721 AGAGTAGTTC ATTATTGAGA CATAAATATG AACACACAGG TAAAAGACCT CATGAGTGTG 
     3781 GAATCTGTAA AAAGGCATTT AAACACAAAC ATCATTTGAT TGAACACATG CGATTACATT 
     3841 CTGGAGAAAA GCCCTATCAA TGTGACAAAT GTGGAAAGCG CTTCTCACAC TCTGGGTCTT 
     3901 ATTCTCAACA CATGAATCAT CGCTACTCCT ACTGTAAGAG AGAAGCGGAA GAACGTGACA 
     3961 GCACAGAGCA GGAAGAGGCA GGGCCTGAAA TCCTCTCGAA TGAGCACGTG GGTGCCAGGG 
     4021 CGTCTCCCTC ACAGGGCGAC TCGGACGAGA GAGAGAGTTT GACAAGGGAA GAGGATGAAG 
     4081 ACAGTGAAAA AGAGGAAGAG GAGGAGGATA AAGAGATGGA AGAATTGCAG GAAGAAAAAG 
     4141 AATGTGAAAA ACCACAAGGG GATGAGGAAG AGGAGGAGGA GGAGGAAGAA GTGGAAGAAG 
     4201 AAGAGGTAGA AGAGGCAGAG AATGAGGGAG AAGAAGCAAA AACTGAAGGT CTGATGAAGG 
     4261 ATGACAGGGC TGAAAGTCAA GCAAGCAGCT TAGGACAAAA AGTAGGCGAG AGTAGTGAGC 
     4321 AAGTGTCTGA AGAAAAGACA AATGAAGCCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4381 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4441 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4501 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4561 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4621 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4681 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4741 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4801 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4861 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4921 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4981 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5041 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5101 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5161 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5221 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5281 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5341 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5401 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5461 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5521 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5581 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5641 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5701 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5761 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5821 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5881 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5941 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6001 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6061 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6121 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6181 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6241 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6301 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6361 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6421 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6481 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6541 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6601 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6661 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6721 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6781 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6841 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6901 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6961 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7021 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7081 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7141 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7201 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7261 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7321 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7381 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7441 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7501 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7561 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7621 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7681 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7741 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7801 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7861 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7921 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7981 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8041 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8101 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8161 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//