LOCUS       RC230650                8193 bp    DNA     circular  SYN 
DEFINITION  ZEB1 (Myc-DDK-tagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 6
ACCESSION   NM_001174093
VERSION     NM_001174093.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4350..4441 
                     /label="Myc-DDK"  
     misc_feature    4471..5056 
                     /label="PolyA signal"  
     misc_feature    4528..4547 
                     /label="Restriction Enzyme"  
     misc_feature    5332..6004 
                     /label="ColE1"  
     misc_feature    6434..7228 
                     /label="Kan/Neo"  
     misc_feature    7263..7565 
                     /label="SV40 ori"  
     misc_feature    7651..8128 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4332..4338 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4332 
                     /label="ZEB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGATGGC CCCAGGTGTA AGCGCAGAAA GCAGGCGAAC CCGCGGCGCA 
     1081 ATAACGTTAC AAATTATAAT ACTGTGGTAG AAACAAATTC AGATTCAGAT GATGAAGACA 
     1141 AACTGCATAT TGTGGAAGAA GAAAGTGTTA CAGATGCAGC TGACTGTGAA GGTGTACCAG 
     1201 AGGATGACCT GCCAACAGAC CAGACAGTGT TACCAGGGAG GAGCAGTGAA AGAGAAGGGA 
     1261 ATGCTAAGAA CTGCTGGGAG GATGACATAA AAGATGATGA ATGCGAGTCA GATGCAGAAA 
     1321 ATGAGCAAAA CCATGATCCT AATGTTGAAG AGTTTCTACA ACAACAAGAC ACTGCTGTCA 
     1381 TTTTTCCTGA GGCACCTGAA GAGGACCAGA GGCAGGGCAC ACCAGAAGCC AGTGGTCATG 
     1441 ATGAAAATGG AACACCAGAT GCATTTTCAC AATTACTCAC CTGTCCATAT TGTGATAGAG 
     1501 GCTATAAACG CTTTACCTCT CTGAAAGAAC ACATTAAATA TCGTCATGAA AAGAATGAAG 
     1561 ATAACTTTAG TTGCTCCCTG TGCAGTTACA CCTTTGCATA CAGAACCCAA CTTGAACGTC 
     1621 ACATGACATC ACATAAATCA GGAAGAGATC AAAGACATGT GACGCAGTCT GGGTGTAATC 
     1681 GTAAATTCAA ATGCACTGAG TGTGGAAAAG CTTTCAAATA CAAACATCAC CTAAAAGAGC 
     1741 ACTTAAGAAT TCACAGTGGA GAGAAGCCAT ATGAATGCCC AAACTGCAAG AAACGCTTTT 
     1801 CCCATTCTGG CTCCTATAGC TCACACATAA GCAGTAAGAA ATGTATCAGC TTGATACCTG 
     1861 TGAATGGGCG ACCAAGAACA GGACTCAAGA CATCTCAGTG TTCTTCACCG TCTCTTTCAG 
     1921 CATCACCAGG CAGTCCCACA CGACCACAGA TACGGCAAAA GATAGAGAAT AAACCCCTTC 
     1981 AAGAACAACT TTCTGTTAAC CAAATTAAAA CTGAACCTGT GGATTATGAA TTCAAACCCA 
     2041 TAGTGGTTGC TTCAGGAATC AACTGTTCAA CCCCTTTACA AAATGGGGTT TTCACTGGTG 
     2101 GTGGCCCATT ACAGGCAACC AGTTCTCCTC AGGGCATGGT GCAAGCTGTT GTTCTGCCAA 
     2161 CAGTTGGTTT GGTGTCTCCC ATAAGTATCA ATTTAAGTGA TATTCAGAAT GTACTTAAAG 
     2221 TGGCGGTAGA TGGTAATGTA ATAAGGCAAG TGTTGGAGAA TAATCAAGCC AATCTTGCAT 
     2281 CCAAAGAACA AGAAACAATC AATGCTTCAC CCATACAACA AGGTGGCCAT TCTGTTATTT 
     2341 CAGCCATCAG TCTTCCTTTG GTTGATCAAG ATGGAACAAC CAAAATTATC ATCAACTACA 
     2401 GTCTTGAGCA GCCTAGCCAA CTTCAAGTTG TTCCTCAAAA TTTAAAAAAA GAAAATCCAG 
     2461 TCGCTACAAA CAGTTGTAAA AGTGAAAAGT TACCAGAAGA TCTTACTGTT AAGTCTGAGA 
     2521 AGGACAAAAG CTTTGAAGGG GGGGTGAATG ATAGCACTTG TCTTCTGTGT GATGATTGTC 
     2581 CAGGAGATAT TAATGCACTT CCAGAATTAA AGCACTATGA CCTAAAGCAG CCTACTCAGC 
     2641 CTCCTCCACT CCCTGCAGCA GAAGCTGAGA AGCCTGAGTC CTCTGTTTCA TCAGCTACTG 
     2701 GAGATGGCAA TTTGTCTCCT AGTCAGCCAC CTTTAAAGAA CCTCTTGTCT CTCCTAAAAG 
     2761 CATATTATGC TTTGAATGCA CAACCAAGTG CAGAAGAGCT CTCAAAAATT GCTGATTCAG 
     2821 TAAACCTACC ACTGGATGTA GTAAAAAAGT GGTTTGAAAA GATGCAAGCT GGACAGATTT 
     2881 CAGTGCAGTC TTCTGAACCA TCTTCTCCTG AACCAGGCAA AGTAAATATC CCTGCCAAGA 
     2941 ACAATGATCA GCCTCAATCT GCAAATGCAA ATGAACCCCA GGACAGCACA GTAAATCTAC 
     3001 AAAGTCCTTT GAAGATGACT AACTCCCCAG TTTTACCAGT GGGATCAACC ACCAATGGTT 
     3061 CCAGAAGTAG TACACCATCC CCATCACCTC TAAACCTTTC CTCATCCAGA AATACACAGG 
     3121 GTTACTTGTA CACAGCTGAG GGTGCACAAG AAGAGCCACA AGTAGAACCT CTTGATCTTT 
     3181 CACTACCAAA GCAACAGGGA GAATTATTAG AAAGGTCAAC TATCACTAGT GTTTACCAGA 
     3241 ACAGTGTTTA TTCTGTCCAG GAAGAACCCT TGAACTTGTC TTGCGCAAAA AAGGAGCCAC 
     3301 AAAAGGACAG TTGTGTTACA GACTCAGAAC CAGTTGTAAA TGTAATCCCA CCAAGTGCCA 
     3361 ACCCCATAAA TATCGCTATA CCTACAGTCA CTGCCCAGTT ACCCACAATC GTGGCCATTG 
     3421 CTGACCAGAA CAGTGTTCCA TGCTTAAGAG CGCTAGCTGC CAATAAGCAA ACGATTCTGA 
     3481 TTCCCCAGGT GGCATACACC TACTCAACTA CGGTCAGCCC TGCAGTCCAA GAACCACCCT 
     3541 TGAAAGTGAT CCAGCCAAAT GGAAATCAGG ATGAAAGACA AGATACTAGC TCAGAAGGAG 
     3601 TATCAAATGT AGAGGATCAG AATGACTCTG ATTCTACACC GCCCAAAAAG AAAATGCGGA 
     3661 AGACAGAAAA TGGAATGTAT GCTTGTGATT TGTGTGACAA GATATTCCAA AAGAGTAGTT 
     3721 CATTATTGAG ACATAAATAT GAACACACAG GTAAAAGACC TCATGAGTGT GGAATCTGTA 
     3781 AAAAGGCATT TAAACACAAA CATCATTTGA TTGAACACAT GCGATTACAT TCTGGAGAAA 
     3841 AGCCCTATCA ATGTGACAAA TGTGGAAAGC GCTTCTCACA CTCTGGGTCT TATTCTCAAC 
     3901 ACATGAATCA TCGCTACTCC TACTGTAAGA GAGAAGCGGA AGAACGTGAC AGCACAGAGC 
     3961 AGGAAGAGGC AGGGCCTGAA ATCCTCTCGA ATGAGCACGT GGGTGCCAGG GCGTCTCCCT 
     4021 CACAGGGCGA CTCGGACGAG AGAGAGAGTT TGACAAGGGA AGAGGATGAA GACAGTGAAA 
     4081 AAGAGGAAGA GGAGGAGGAT AAAGAGATGG AAGAATTGCA GGAAGAAAAA GAATGTGAAA 
     4141 AACCACAAGG GGATGAGGAA GAGGAGGAGG AGGAGGAAGA AGTGGAAGAA GAAGAGGTAG 
     4201 AAGAGGCAGA GAATGAGGGA GAAGAAGCAA AAACTGAAGG TCTGATGAAG GATGACAGGG 
     4261 CTGAAAGTCA AGCAAGCAGC TTAGGACAAA AAGTAGGCGA GAGTAGTGAG CAAGTGTCTG 
     4321 AAGAAAAGAC AAATGAAGCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4381 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4441 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4501 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4561 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4621 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4681 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4741 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4801 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4861 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4921 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4981 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5041 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5101 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5161 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5221 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5281 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5341 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5461 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5521 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5581 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5641 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5761 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5821 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5881 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5941 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6001 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6061 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6121 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6181 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6241 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6301 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6361 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6421 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6481 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6541 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6601 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6661 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6721 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6781 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6841 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6901 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6961 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7021 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7081 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7141 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7201 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7261 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7321 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7381 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7441 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7501 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7561 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7621 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7681 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7741 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7801 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7861 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7921 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7981 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8041 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8101 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8161 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//