LOCUS       RC230640                8079 bp    DNA     circular  SYN 
DEFINITION  MIB2 (Myc-DDK-tagged)-Human mindbomb homolog 2 (Drosophila) (MIB2), transcript variant 2
ACCESSION   NM_001170686
VERSION     NM_001170686.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4236..4327 
                     /label="Myc-DDK"  
     misc_feature    4357..4942 
                     /label="PolyA signal"  
     misc_feature    4414..4433 
                     /label="Restriction Enzyme"  
     misc_feature    5218..5890 
                     /label="ColE1"  
     misc_feature    6320..7114 
                     /label="Kan/Neo"  
     misc_feature    7149..7451 
                     /label="SV40 ori"  
     misc_feature    7537..8014 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4218..4224 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4218 
                     /label="MIB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGGGCG CTCCGGCGGG GGCGGGCCCT GGGCTCCCGC CCTTCGGGTC 
     1081 CCACAGTTTC CAGCCGCCGC TCTCCTCAGT GCCCGGTGGC CCAGGAGGGC CTGGGAGCCC 
     1141 GAAGCCGTCC CCGAGTCGCT CCTAGGTCAC TGGCGCGATG CGGGCCGTCC TCTCGGCTGA 
     1201 TGGGTTGGAA GCCCAGCGAG GCTAGAGGCC AGTCCCAAAG TTTCCAGGCA TCAGGGCTGC 
     1261 AGCCCAGGAG CCTCAAGGCG GCCCGGCGGG CGACTGGACG GCCGGACAGG TCCCGAGCAG 
     1321 CCCGGCCCAC CATGGACCCC TCTGCCCACA GGTCCCGAGC AGCCCCGCCC AACATGGACC 
     1381 CAGACCCCCA GGCGGGCGTG CAGGTGGGCA TGCGGGTGGT GCGCGGCGTG GACTGGAAGT 
     1441 GGGGCCAGCA GGACGGCGGC GAGGGCGGCG TGGGCACGGT GGTGGAGCTT GGCCGCCACG 
     1501 GCAGCCCCTC GACACCCGAC CGCACAGTGG TCGTGCAGTG GGACCAGGGC ACGCGCACCA 
     1561 ACTACCGCGC CGGCTACCAG GGCGCGCACG ACCTGCTGCT GTACGACAAC GCCCAGATCG 
     1621 GCGTCCGGCA CCCCAACATC ATCTGTGACT GCTGCAAGAA GCACGGGCTG CGGGGGATGC 
     1681 GCTGGAAGTG CCGTGTGTGC CTGGACTACG ACCTCTGCAC GCAGTGCTAC ATGCACAACA 
     1741 AGCATGAGCT CGCCCACGCC TTCGACCGCT ACGAGACCGC TCACTCGCGC CCTGTCACAC 
     1801 TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CACTAAGGGG CATCTTCCAG GGAGCGAAGG 
     1861 TGGTGCGAGG CCCCGACTGG GAGTGGGGCT CACAGGATGG AGGGGAAGGG AAACCGGGCC 
     1921 GTGTGGTGGA CATCCGTGGC TGGGATGTGG AGACAGGCCG GAGTGTGGCC AGCGTGACGT 
     1981 GGGCTGATGG TACCACCAAT GTGTACCGTG TGGGCCACAA GGGCAAGGTG GACCTCAAGT 
     2041 GTGTGGGCGA GGCAGCGGGC GGCTTCTACT ACAAGGACCA CCTCCCAAGG CTCGGCAAGC 
     2101 CGGCGGAGCT GCAGCGCAGG GTGAGTGCTG ACAGCCAGCC CTTCCAGCAC GGGGACAAGG 
     2161 TCAAGTGTCT GCTGGACACT GATGTCCTGC GGGAGATGCA GGAAGGCCAC GGCGGCTGGA 
     2221 ACCCCAGGAT GGCGGAGACG GGCACCGTGC ATCGTATCAC GGACCGCGGG GACGTGCGCG 
     2281 TGCAGTTCAA CCACGAGACG CGCTGGACCT TCCACCCCGG GGCGCTCACC AAGCACCACT 
     2341 CCTTCTGGGT GGGCGACGTG GTCCGGGTCA TCGGCGACCT TGACACAGTG AAGCGGCTGC 
     2401 AGGCTGGGCA TGGCGAGTGG ACGGACGACA TGGCCCCTGC CCTGGGCCGC GTCGGGAAGG 
     2461 TGGTGAAAGT GTTTGGAGAC GGGAACCTGC GTGTAGCAGT CGCTGGTCAG CGGTGGACCT 
     2521 TCAGCCCCTC CTGCCTGGTG GCCTACCGGC CCGAGGAGGA TGCCAACCTG GACGTGGCCG 
     2581 AGCGCGCCCG GGAGAACAAA AGCTCACTGA GCGTGGCCCT GGACAAGCTT CGGGCCCAGA 
     2641 AGAGTGACCC AGAGCACCCG GGAAGGCTGG TGGTGGAGGT GGCGCTGGGT AACGCAGCCC 
     2701 GGGCTCTGGA CCTGCTGCGG AGGCGCCCAG AGCAGGTGGA CACCAAGAAC CAAGGCAGGA 
     2761 CCGCTCTGCA AGTGGCTGCC TACCTGGGCC AGGTGGAGTT GATACGGCTG CTGCTACAAG 
     2821 CCAGGGCGGG CGTGGACCTG CCGGACGACG AGGGCAACAC GGCACTGCAC TACGCGGCCC 
     2881 TGGGGAACCA GCCCGAGGCC ACCAGGGTGC TCCTGAGTGC TGGGTGCCGG GCGGACGCCA 
     2941 TCAACAGCAC CCAGAGCACA GCACTGCACG TGGCCGTGCA GAGGGGCTTC CTGGAGGTGG 
     3001 TGCGGGCCCT GTGTGAGCGC GGCTGTGACG TCAACCTGCC CGACGCCCAC TCGGACACGC 
     3061 CCCTGCACTC CGCCATCTCG GCGGGCACTG GAGCCAGCGG CATTGTCGAG GTCCTCACGG 
     3121 AGGTGCCAAA CATCGATGTT ACCGCCACCA ACAGCCAGGG TTTCACCCTG CTGCACCATG 
     3181 CCTCCCTCAA GGGTCACGCG CTAGCTGTGA GAAAGATTCT GGCTCGGGCG CGGCAGCTGG 
     3241 TGGACGCCAA GAAGGAGGAC GGCTTCACGG CGCTGCATCT GGCTGCCCTC AACAACCACC 
     3301 GCGAGGTGGC CCAGATCCTC ATCCGGGAGG GCCGCTGTGA CGTGAACGTG CGCAACCGGA 
     3361 AGCTGCAGTC CCCGCTGCAT CTCGCCGTGC AACAGGCCCA CGTGGGGCTG GTGCCGCTAC 
     3421 TGGTGGACGC TGGGTGCAGT GTCAACGCCG AGGACGAGGA GGGGGACACA GCCCTGCACG 
     3481 TGGCGCTGCA GCGTCATCAG CTGCTGCCCC TGGTGGCTGA TGGGGCCGGG GGGGACCCAG 
     3541 GGCCCTTGCA GCTGCTGTCC AGGCTACAGG CCTCGGGCCT CCCCGGCAGC GCGGAGCTGA 
     3601 CGGTGGGCGC GGCGGTCGCC TGCTTCCTGG CGCTGGAGGG CGCCGACGTG AGCTACACCA 
     3661 ACCACCGCGG TCGGAGCCCG CTGGACCTGG CCGCCGAGGG TCGCGTGCTC AAGGCCCTTC 
     3721 AGGGCTGCGC CCAGCGCTTC CGGGAGCGGC AGGCGGGCGG GGGCGCGGCC CCGGGCCCCA 
     3781 GGCAAACGCT CGGGACCCCC AACACCGTGA CGAACCTGCA CGTGGGCGCC GCGCCGGGGC 
     3841 CCGAGGCCGC TGAGTGCCTG GTGTGCTCCG AGCTGGCGCT GCTGGTGCTG TTCTCGCCGT 
     3901 GCCAGCACCG CACCGTGTGT GAGGAGTGCG CGCGCAGGAT GAAGAAGTGC ATCAGGTGCC 
     3961 AGGTGGTCGT CAGCAAGAAA CTGCGCCCAG ACGGCTCTGA GGTGGCGAGC GCCGCCCCCG 
     4021 CCCCCGGCCC GCCGCGCCAG CTGGTGGAGG AGCTGCAGAG CCGCTACCGG CAGATGGAGG 
     4081 AACGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTCGTGTTC CAGTGCGGCC 
     4141 ACGGCGCATG CGCCCCCTGC GGCTCCGCGC TCAGCGCCTG CCCCATCTGC CGCCAGCCCA 
     4201 TCCGCGACCG CATCCAGATC TTCGTGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4261 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4321 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4381 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4441 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4501 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4561 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4621 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4681 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4741 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4801 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4861 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4921 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4981 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5041 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5101 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5161 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5221 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5281 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5341 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5401 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5461 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5521 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5581 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5641 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5701 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5761 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5821 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5881 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5941 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6001 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6061 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6121 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6181 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6241 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6301 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6361 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6421 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6481 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6541 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6601 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6661 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6721 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6781 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6841 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6901 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6961 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7021 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7081 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7141 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7201 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7261 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7321 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7501 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7561 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7621 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7681 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7741 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7801 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7861 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7921 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7981 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8041 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//