LOCUS       RC230639                8076 bp    DNA     circular  SYN 
DEFINITION  GLI1 (Myc-DDK-tagged)-Human GLI family zinc finger 1 (GLI1), transcript variant 3
ACCESSION   NM_001167609
VERSION     NM_001167609.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4233..4324 
                     /label="Myc-DDK"  
     misc_feature    4354..4939 
                     /label="PolyA signal"  
     misc_feature    4411..4430 
                     /label="Restriction Enzyme"  
     misc_feature    5215..5887 
                     /label="ColE1"  
     misc_feature    6317..7111 
                     /label="Kan/Neo"  
     misc_feature    7146..7448 
                     /label="SV40 ori"  
     misc_feature    7534..8011 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4215..4221 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4215 
                     /label="GLI1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCAACTCG ATGACCCCAC CACCAATCAG TAGCTATGGC GAGCCCTGCT 
     1081 GTCTCCGGCC CCTCCCCAGT CAGGGGGCCC CCAGTGTGGG GACAGAAGTC AAGTTGACCA 
     1141 AGAAGCGGGC ACTGTCCATC TCACCTCTGT CGGATGCCAG CCTGGACCTG CAGACGGTTA 
     1201 TCCGCACCTC ACCCAGCTCC CTCGTAGCTT TCATCAACTC GCGATGCACA TCTCCAGGAG 
     1261 GCTCCTACGG TCATCTCTCC ATTGGCACCA TGAGCCCATC TCTGGGATTC CCAGCCCAGA 
     1321 TGAATCACCA AAAAGGGCCC TCGCCTTCCT TTGGGGTCCA GCCTTGTGGT CCCCATGACT 
     1381 CTGCCCGGGG TGGGATGATC CCACATCCTC AGTCCCGGGG ACCCTTCCCA ACTTGCCAGC 
     1441 TGAAGTCTGA GCTGGACATG CTGGTTGGCA AGTGCCGGGA GGAACCCTTG GAAGGTGATA 
     1501 TGTCCAGCCC CAACTCCACA GGCATACAGG ATCCCCTGTT GGGGATGCTG GATGGGCGGG 
     1561 AGGACCTCGA GAGAGAGGAG AAGCGTGAGC CTGAATCTGT GTATGAAACT GACTGCCGTT 
     1621 GGGATGGCTG CAGCCAGGAA TTTGACTCCC AAGAGCAGCT GGTGCACCAC ATCAACAGCG 
     1681 AGCACATCCA CGGGGAGCGG AAGGAGTTCG TGTGCCACTG GGGGGGCTGC TCCAGGGAGC 
     1741 TGAGGCCCTT CAAAGCCCAG TACATGCTGG TGGTTCACAT GCGCAGACAC ACTGGCGAGA 
     1801 AGCCACACAA GTGCACGTTT GAAGGGTGCC GGAAGTCATA CTCACGCCTC GAAAACCTGA 
     1861 AGACGCACCT GCGGTCACAC ACGGGTGAGA AGCCATACAT GTGTGAGCAC GAGGGCTGCA 
     1921 GTAAAGCCTT CAGCAATGCC AGTGACCGAG CCAAGCACCA GAATCGGACC CATTCCAATG 
     1981 AGAAGCCGTA TGTATGTAAG CTCCCTGGCT GCACCAAACG CTATACAGAT CCTAGCTCGC 
     2041 TGCGAAAACA TGTCAAGACA GTGCATGGTC CTGACGCCCA TGTGACCAAA CGGCACCGTG 
     2101 GGGATGGCCC CCTGCCTCGG GCACCATCCA TTTCTACAGT GGAGCCCAAG AGGGAGCGGG 
     2161 AAGGAGGTCC CATCAGGGAG GAAAGCAGAC TGACTGTGCC AGAGGGTGCC ATGAAGCCAC 
     2221 AGCCAAGCCC TGGGGCCCAG TCATCCTGCA GCAGTGACCA CTCCCCGGCA GGGAGTGCAG 
     2281 CCAATACAGA CAGTGGTGTG GAAATGACTG GCAATGCAGG GGGCAGCACT GAAGACCTCT 
     2341 CCAGCTTGGA CGAGGGACCT TGCATTGCTG GCACTGGTCT GTCCACTCTT CGCCGCCTTG 
     2401 AGAACCTCAG GCTGGACCAG CTACATCAAC TCCGGCCAAT AGGGACCCGG GGTCTCAAAC 
     2461 TGCCCAGCTT GTCCCACACC GGTACCACTG TGTCCCGCCG CGTGGGCCCC CCAGTCTCTC 
     2521 TTGAACGCCG CAGCAGCAGC TCCAGCAGCA TCAGCTCTGC CTATACTGTC AGCCGCCGCT 
     2581 CCTCCCTGGC CTCTCCTTTC CCCCCTGGCT CCCCACCAGA GAATGGAGCA TCCTCCCTGC 
     2641 CTGGCCTTAT GCCTGCCCAG CACTACCTGC TTCGGGCAAG ATATGCTTCA GCCAGAGGGG 
     2701 GTGGTACTTC GCCCACTGCA GCATCCAGCC TGGATCGGAT AGGTGGTCTT CCCATGCCTC 
     2761 CTTGGAGAAG CCGAGCCGAG TATCCAGGAT ACAACCCCAA TGCAGGGGTC ACCCGGAGGG 
     2821 CCAGTGACCC AGCCCAGGCT GCTGACCGTC CTGCTCCAGC TAGAGTCCAG AGGTTCAAGA 
     2881 GCCTGGGCTG TGTCCATACC CCACCCACTG TGGCAGGGGG AGGACAGAAC TTTGATCCTT 
     2941 ACCTCCCAAC CTCTGTCTAC TCACCACAGC CCCCCAGCAT CACTGAGAAT GCTGCCATGG 
     3001 ATGCTAGAGG GCTACAGGAA GAGCCAGAAG TTGGGACCTC CATGGTGGGC AGTGGTCTGA 
     3061 ACCCCTATAT GGACTTCCCA CCTACTGATA CTCTGGGATA TGGGGGACCT GAAGGGGCAG 
     3121 CAGCTGAGCC TTATGGAGCG AGGGGTCCAG GCTCTCTGCC TCTTGGGCCT GGTCCACCCA 
     3181 CCAACTATGG CCCCAACCCC TGTCCCCAGC AGGCCTCATA TCCTGACCCC ACCCAAGAAA 
     3241 CATGGGGTGA GTTCCCTTCC CACTCTGGGC TGTACCCAGG CCCCAAGGCT CTAGGTGGAA 
     3301 CCTACAGCCA GTGTCCTCGA CTTGAACATT ATGGACAAGT GCAAGTCAAG CCAGAACAGG 
     3361 GGTGCCCAGT GGGGTCTGAC TCCACAGGAC TGGCACCCTG CCTCAATGCC CACCCCAGTG 
     3421 AGGGGCCCCC ACATCCACAG CCTCTCTTTT CCCATTACCC CCAGCCCTCT CCTCCCCAAT 
     3481 ATCTCCAGTC AGGCCCCTAT ACCCAGCCAC CCCCTGATTA TCTTCCTTCA GAACCCAGGC 
     3541 CTTGCCTGGA CTTTGATTCC CCCACCCATT CCACAGGGCA GCTCAAGGCT CAGCTTGTGT 
     3601 GTAATTATGT TCAATCTCAA CAGGAGCTAC TGTGGGAGGG TGGGGGCAGG GAAGATGCCC 
     3661 CCGCCCAGGA ACCTTCCTAC CAGAGTCCCA AGTTTCTGGG GGGTTCCCAG GTTAGCCCAA 
     3721 GCCGTGCTAA AGCTCCAGTG AACACATATG GACCTGGCTT TGGACCCAAC TTGCCCAATC 
     3781 ACAAGTCAGG TTCCTATCCC ACCCCTTCAC CATGCCATGA AAATTTTGTA GTGGGGGCAA 
     3841 ATAGGGCTTC ACATAGGGCA GCAGCACCAC CTCGACTTCT GCCCCCATTG CCCACTTGCT 
     3901 ATGGGCCTCT CAAAGTGGGA GGCACAAACC CCAGCTGTGG TCATCCTGAG GTGGGCAGGC 
     3961 TAGGAGGGGG TCCTGCCTTG TACCCTCCTC CCGAAGGACA GGTATGTAAC CCCCTGGACT 
     4021 CTCTTGATCT TGACAACACT CAGCTGGACT TTGTGGCTAT TCTGGATGAG CCCCAGGGGC 
     4081 TGAGTCCTCC TCCTTCCCAT GATCAGCGGG GCAGCTCTGG ACATACCCCA CCTCCCTCTG 
     4141 GGCCCCCCAA CATGGCTGTG GGCAACATGA GTGTCTTACT GAGATCCCTA CCTGGGGAAA 
     4201 CAGAATTCCT CAACTCTAGT GCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4261 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4321 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4381 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4441 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4501 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4561 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4621 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4681 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4741 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4801 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4861 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4921 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4981 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5041 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5101 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5161 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5221 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5281 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5341 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5401 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5461 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5521 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5581 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5641 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5701 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5761 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5821 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5881 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5941 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6001 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6061 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6121 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6181 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6241 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6301 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6361 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6421 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6481 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6541 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6601 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6661 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6721 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6781 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6841 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6901 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6961 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7021 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7081 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7141 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7201 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7261 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7321 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7381 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7441 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7501 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7561 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7621 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7681 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7741 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7801 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7861 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7921 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7981 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8041 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//