LOCUS       RC230633                8070 bp    DNA     circular  SYN 
DEFINITION  PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 1
ACCESSION   NM_001184896
VERSION     NM_001184896.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4225..4316 
                     /label="Myc-DDK"  
     misc_feature    4346..4931 
                     /label="PolyA signal"  
     misc_feature    4403..4422 
                     /label="Restriction Enzyme"  
     misc_feature    5207..5879 
                     /label="ColE1"  
     misc_feature    6309..7103 
                     /label="Kan/Neo"  
     misc_feature    7138..7440 
                     /label="SV40 ori"  
     misc_feature    7526..8003 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4200..4207 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4200 
                     /label="PHF8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACAGGAGC CGCGCTATCG TCCAGAGAGG ACGCGTGCTG CCGCCTCCCG 
     1081 CCCCTCTTGA CACGACGAAC CTGGCCGGCC GCAGAACGCT CCAGGGCCGA GCGAAGATGG 
     1141 CCTCGGTGCC GGTGTATTGC CTCTGCCGGC TGCCTTACGA TGTGACCCGC TTCATGATCG 
     1201 AGTGTGACAT GTGCCAGGAC TGGTTTCATG GCAGTTGTGT TGGTGTTGAA GAGGAGAAGG 
     1261 CTGCTGACAT TGACCTCTAC CACTGCCCCA ACTGTGAAGT CTTGCATGGG CCCTCCATTA 
     1321 TGAAAAAACG CCGTGGATCT TCAAAGGGGC ATGATACACA CAAGGGGAAA CCAGTGAAGA 
     1381 CCGGGAGCCC TACGTTCGTC AGAGAGCTCC GGAGTAGGAC TTTTGACAGC TCAGATGAAG 
     1441 TGATTCTGAA GCCCACTGGA AATCAACTGA CCGTGGAATT CCTGGAAGAA AATAGCTTCA 
     1501 GTGTGCCCAT CCTGGTCCTG AAGAAGGATG GGTTGGGCAT GACGCTGCCC TCGCCATCAT 
     1561 TCACTGTGAG GGATGTTGAA CACTATGTTG GTTCTGACAA AGAGATTGAT GTGATTGATG 
     1621 TGACCCGCCA GGCTGACTGC AAGATGAAGC TTGGTGATTT TGTGAAATAC TATTACAGCG 
     1681 GGAAGAGGGA GAAAGTCCTC AATGTCATTA GTTTGGAATT CTCTGATACC AGACTTTCTA 
     1741 ACCTTGTGGA GACACCGAAG ATTGTTCGAA AGCTGTCATG GGTCGAAAAC TTGTGGCCAG 
     1801 AGGAATGTGT CTTTGAGAGA CCCAATGTAC AGAAGTACTG CCTCATGAGT GTGCGAGATA 
     1861 GCTATACAGA CTTTCACATT GACTTTGGTG GCACCTCTGT CTGGTACCAT GTACTCAAGG 
     1921 GTGAAAAGAT CTTCTACCTG ATCCGCCCAA CAAATGCCAA TCTGACTCTC TTTGAGTGCT 
     1981 GGAGCAGTTC CTCTAATCAG AATGAGATGT TCTTTGGGGA CCAGGTGGAC AAGTGCTACA 
     2041 AGTGTTCCGT GAAGCAAGGA CAGACACTTT TCATTCCCAC AGGGTGGATC CATGCTGTGC 
     2101 TGACGCCTGT GGACTGCCTT GCCTTTGGAG GGAACTTCTT ACACAGCCTT AACATCGAGA 
     2161 TGCAGCTCAA AGCCTATGAG ATTGAGAAGC GGCTGAGCAC AGCAGACCTC TTCAGATTCC 
     2221 CCAACTTTGA GACCATCTGT TGGTATGTGG GAAAGCACAT CCTGGACATC TTTCGCGGTT 
     2281 TGCGAGAGAA CAGGAGACAC CCTGCCTCCT ACCTGGTCCA TGGTGGCAAA GCCTTGAACT 
     2341 TGGCCTTTAG AGCCTGGACA AGGAAAGAAG CTCTGCCAGA CCATGAGGAT GAGATCCCGG 
     2401 AGACAGTGCG AACCGTACAG CTCATTAAAG ATCTGGCCAG GGAGATCCGC CTGGTGGAAG 
     2461 ACATCTTCCA ACAGAACGTT GGGAAGACGA GCAATATCTT TGGGCTGCAG AGGATCTTCC 
     2521 CAGCCGGCTC CATTCCCCTA ACCAGGCCAG CCCATTCCAC TTCAGTGTCC ATGTCCAGGC 
     2581 TGTCACTGCC CTCCAAAAAT GGTTCAAAGA AGAAAGGCCT GAAGCCCAAG GAACTCTTCA 
     2641 AGAAGGCAGA GCGAAAGGGC AAGGAGAGTT CAGCCTTGGG GCCTGCTGGC CAGTTGAGCT 
     2701 ATAATCTCAT GGACACATAC AGTCATCAGG CACTGAAGAC AGGCTCTTTC CAGAAAGCAA 
     2761 AGTTCAACAT CACTGGTGCC TGCTTGAATG ACTCAGATGA CGACTCACCA GACTTGGACC 
     2821 TTGATGGAAA TGAGAGCCCA TTGGCCCTAT TGATGTCTAA CGGCAGTACG AAAAGGGTGA 
     2881 AGAGTTTATC CAAATCTCGG CGAACCAAGA TAGCAAAGAA GGTAGACAAG GCTAGGCTGA 
     2941 TGGCAGAACA GGTGATGGAA GACGAATTTG ACTTGGATTC AGATGATGAG CTGCAGATTG 
     3001 ACGAGAGATT GGGAAAGGAG AAGGCGACCC TGATAATAAG ACCAAAATTT CCCCGGAAAT 
     3061 TGCCCCGTGC GAAGCCTTGC TCTGACCCCA ACCGAGTTCG TGAACCAGGA GAAGTTGAGT 
     3121 TTGACATTGA GGAGGACTAT ACAACAGATG AGGACATGGT GGAAGGGGTT GAAGGCAAGC 
     3181 TTGGGAATGG TAGTGGCGCT GGTGGCATTC TTGATCTGCT CAAGGCCAGC AGGCAGGTGG 
     3241 GGGGACCTGA CTATGCTGCC CTCACCGAGG CCCCAGCTTC TCCCAGCACT CAGGAGGCCA 
     3301 TCCAGGGCAT GCTGTGCATG GCCAACCTGC AGTCCTCATC GTCCTCACCG GCTACCTCTA 
     3361 GCCTGCAGGC CTGGTGGACT GGGGGACAGG ATCGAAGCAG TGGGAGCTCC AGCAGTGGGC 
     3421 TGGGCACAGT GTCTAACAGT CCTGCTTCCC AGCGCACCCC AGGGAAGCGG CCCATCAAGC 
     3481 GGCCAGCATA CTGGAGAACC GAGAGCGAGG AGGAGGAGGA GAACGCCAGT CTGGATGAAC 
     3541 AGGACAGCTT GGGAGCGTGC TTCAAGGATG CAGAGTATAT CTATCCTTCA CTGGAGTCTG 
     3601 ATGATGATGA CCCTGCTTTG AAATCTCGAC CCAAGAAAAA GAAGAATTCA GATGATGCTC 
     3661 CATGGAGTCC TAAAGCCCGC GTGACCCCAA CTCTGCCGAA GCAGGACCGT CCTGTGCGTG 
     3721 AGGGGACCCG GGTAGCCTCT ATTGAGACAG GTTTGGCTGC AGCAGCTGCA AAGCTGGCCC 
     3781 AGCAGGAGCT ACAGAAGGCC CAAAAGAAGA AATATATCAA GAAGAAGCCT TTGCTGAAGG 
     3841 AGGTAGAACA GCCTCGCCCT CAAGACTCCA ATCTCAGTCT GACAGTACCA GCCCCCACTG 
     3901 TGGCTGCCAC ACCACAACTT GTCACCTCCT CCTCACCCCT GCCTCCTCCT GAGCCTAAAC 
     3961 AAGAGGCCCT GTCAGGAAGT CTCGCTGACC ATGAGTACAC CGCTCGTCCC AATGCCTTTG 
     4021 GCATGGCCCA GGCAAACCGC AGCACCACAC CTATGGCCCC CGGTGTCTTC TTGACCCAGC 
     4081 GGCGCCCTTC AGTTGGCTCC CAGAGCAATC AGGCAGGACA AGGAAAGCGT CCCAAAAAGG 
     4141 GCCTGGCCAC AGCAAAGCAG AGACTCGGCC GTATCCTGAA AATCCACAGA AATGGCAAAC 
     4201 TACTTCTGAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4261 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4321 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4381 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4441 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4501 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4561 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4621 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4681 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4741 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4801 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4861 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4921 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4981 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5041 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5101 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5161 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5221 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5281 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5341 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5401 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5461 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5521 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5581 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5641 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5701 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5761 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5821 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5881 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5941 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6001 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6061 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6121 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6181 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6241 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6301 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6361 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6421 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6481 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6541 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6601 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6661 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6721 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6781 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6841 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6901 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6961 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7021 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7081 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7141 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7201 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7261 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7321 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7381 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7441 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7501 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7561 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7621 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7681 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7741 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7801 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7861 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7921 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7981 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8041 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//