LOCUS       RC230631                8052 bp    DNA     circular  SYN 
DEFINITION  ZEB1 (Myc-DDK-tagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 8
ACCESSION   NM_001174095
VERSION     NM_001174095.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4209..4300 
                     /label="Myc-DDK"  
     misc_feature    4330..4915 
                     /label="PolyA signal"  
     misc_feature    4387..4406 
                     /label="Restriction Enzyme"  
     misc_feature    5191..5863 
                     /label="ColE1"  
     misc_feature    6293..7087 
                     /label="Kan/Neo"  
     misc_feature    7122..7424 
                     /label="SV40 ori"  
     misc_feature    7510..7987 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4191..4197 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4191 
                     /label="ZEB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGATGGC CCCAGGTGTA AGCGCAGAAA GCAGGCGAAC CCGCGGCGCA 
     1081 ATAACGGAAA GGAAGGGCAA GAAATCCTGG GGCCTGAAGC TCAGGCAGAT GAAGCAGGAT 
     1141 GTACAGTAAA AGATGATGAA TGCGAGTCAG ATGCAGAAAA TGAGCAAAAC CATGATCCTA 
     1201 ATGTTGAAGA GTTTCTACAA CAACAAGACA CTGCTGTCAT TTTTCCTGAG GCACCTGAAG 
     1261 AGGACCAGAG GCAGGGCACA CCAGAAGCCA GTGGTCATGA TGAAAATGGA ACACCAGATG 
     1321 CATTTTCACA ATTACTCACC TGTCCATATT GTGATAGAGG CTATAAACGC TTTACCTCTC 
     1381 TGAAAGAACA CATTAAATAT CGTCATGAAA AGAATGAAGA TAACTTTAGT TGCTCCCTGT 
     1441 GCAGTTACAC CTTTGCATAC AGAACCCAAC TTGAACGTCA CATGACATCA CATAAATCAG 
     1501 GAAGAGATCA AAGACATGTG ACGCAGTCTG GGTGTAATCG TAAATTCAAA TGCACTGAGT 
     1561 GTGGAAAAGC TTTCAAATAC AAACATCACC TAAAAGAGCA CTTAAGAATT CACAGTGGAG 
     1621 AGAAGCCATA TGAATGCCCA AACTGCAAGA AACGCTTTTC CCATTCTGGC TCCTATAGCT 
     1681 CACACATAAG CAGTAAGAAA TGTATCAGCT TGATACCTGT GAATGGGCGA CCAAGAACAG 
     1741 GACTCAAGAC ATCTCAGTGT TCTTCACCGT CTCTTTCAGC ATCACCAGGC AGTCCCACAC 
     1801 GACCACAGAT ACGGCAAAAG ATAGAGAATA AACCCCTTCA AGAACAACTT TCTGTTAACC 
     1861 AAATTAAAAC TGAACCTGTG GATTATGAAT TCAAACCCAT AGTGGTTGCT TCAGGAATCA 
     1921 ACTGTTCAAC CCCTTTACAA AATGGGGTTT TCACTGGTGG TGGCCCATTA CAGGCAACCA 
     1981 GTTCTCCTCA GGGCATGGTG CAAGCTGTTG TTCTGCCAAC AGTTGGTTTG GTGTCTCCCA 
     2041 TAAGTATCAA TTTAAGTGAT ATTCAGAATG TACTTAAAGT GGCGGTAGAT GGTAATGTAA 
     2101 TAAGGCAAGT GTTGGAGAAT AATCAAGCCA ATCTTGCATC CAAAGAACAA GAAACAATCA 
     2161 ATGCTTCACC CATACAACAA GGTGGCCATT CTGTTATTTC AGCCATCAGT CTTCCTTTGG 
     2221 TTGATCAAGA TGGAACAACC AAAATTATCA TCAACTACAG TCTTGAGCAG CCTAGCCAAC 
     2281 TTCAAGTTGT TCCTCAAAAT TTAAAAAAAG AAAATCCAGT CGCTACAAAC AGTTGTAAAA 
     2341 GTGAAAAGTT ACCAGAAGAT CTTACTGTTA AGTCTGAGAA GGACAAAAGC TTTGAAGGGG 
     2401 GGGTGAATGA TAGCACTTGT CTTCTGTGTG ATGATTGTCC AGGAGATATT AATGCACTTC 
     2461 CAGAATTAAA GCACTATGAC CTAAAGCAGC CTACTCAGCC TCCTCCACTC CCTGCAGCAG 
     2521 AAGCTGAGAA GCCTGAGTCC TCTGTTTCAT CAGCTACTGG AGATGGCAAT TTGTCTCCTA 
     2581 GTCAGCCACC TTTAAAGAAC CTCTTGTCTC TCCTAAAAGC ATATTATGCT TTGAATGCAC 
     2641 AACCAAGTGC AGAAGAGCTC TCAAAAATTG CTGATTCAGT AAACCTACCA CTGGATGTAG 
     2701 TAAAAAAGTG GTTTGAAAAG ATGCAAGCTG GACAGATTTC AGTGCAGTCT TCTGAACCAT 
     2761 CTTCTCCTGA ACCAGGCAAA GTAAATATCC CTGCCAAGAA CAATGATCAG CCTCAATCTG 
     2821 CAAATGCAAA TGAACCCCAG GACAGCACAG TAAATCTACA AAGTCCTTTG AAGATGACTA 
     2881 ACTCCCCAGT TTTACCAGTG GGATCAACCA CCAATGGTTC CAGAAGTAGT ACACCATCCC 
     2941 CATCACCTCT AAACCTTTCC TCATCCAGAA ATACACAGGG TTACTTGTAC ACAGCTGAGG 
     3001 GTGCACAAGA AGAGCCACAA GTAGAACCTC TTGATCTTTC ACTACCAAAG CAACAGGGAG 
     3061 AATTATTAGA AAGGTCAACT ATCACTAGTG TTTACCAGAA CAGTGTTTAT TCTGTCCAGG 
     3121 AAGAACCCTT GAACTTGTCT TGCGCAAAAA AGGAGCCACA AAAGGACAGT TGTGTTACAG 
     3181 ACTCAGAACC AGTTGTAAAT GTAATCCCAC CAAGTGCCAA CCCCATAAAT ATCGCTATAC 
     3241 CTACAGTCAC TGCCCAGTTA CCCACAATCG TGGCCATTGC TGACCAGAAC AGTGTTCCAT 
     3301 GCTTAAGAGC GCTAGCTGCC AATAAGCAAA CGATTCTGAT TCCCCAGGTG GCATACACCT 
     3361 ACTCAACTAC GGTCAGCCCT GCAGTCCAAG AACCACCCTT GAAAGTGATC CAGCCAAATG 
     3421 GAAATCAGGA TGAAAGACAA GATACTAGCT CAGAAGGAGT ATCAAATGTA GAGGATCAGA 
     3481 ATGACTCTGA TTCTACACCG CCCAAAAAGA AAATGCGGAA GACAGAAAAT GGAATGTATG 
     3541 CTTGTGATTT GTGTGACAAG ATATTCCAAA AGAGTAGTTC ATTATTGAGA CATAAATATG 
     3601 AACACACAGG TAAAAGACCT CATGAGTGTG GAATCTGTAA AAAGGCATTT AAACACAAAC 
     3661 ATCATTTGAT TGAACACATG CGATTACATT CTGGAGAAAA GCCCTATCAA TGTGACAAAT 
     3721 GTGGAAAGCG CTTCTCACAC TCTGGGTCTT ATTCTCAACA CATGAATCAT CGCTACTCCT 
     3781 ACTGTAAGAG AGAAGCGGAA GAACGTGACA GCACAGAGCA GGAAGAGGCA GGGCCTGAAA 
     3841 TCCTCTCGAA TGAGCACGTG GGTGCCAGGG CGTCTCCCTC ACAGGGCGAC TCGGACGAGA 
     3901 GAGAGAGTTT GACAAGGGAA GAGGATGAAG ACAGTGAAAA AGAGGAAGAG GAGGAGGATA 
     3961 AAGAGATGGA AGAATTGCAG GAAGAAAAAG AATGTGAAAA ACCACAAGGG GATGAGGAAG 
     4021 AGGAGGAGGA GGAGGAAGAA GTGGAAGAAG AAGAGGTAGA AGAGGCAGAG AATGAGGGAG 
     4081 AAGAAGCAAA AACTGAAGGT CTGATGAAGG ATGACAGGGC TGAAAGTCAA GCAAGCAGCT 
     4141 TAGGACAAAA AGTAGGCGAG AGTAGTGAGC AAGTGTCTGA AGAAAAGACA AATGAAGCCA 
     4201 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4261 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4321 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4381 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4441 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4501 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4561 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4621 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4681 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4741 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4801 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4861 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4921 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4981 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5041 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5101 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5161 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5221 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5281 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5341 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5401 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5461 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5521 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5581 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5641 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5701 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5761 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5821 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5881 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5941 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6001 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6061 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6121 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6181 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6241 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6301 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6361 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6421 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6481 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6541 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6601 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6661 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6721 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6781 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6841 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6901 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6961 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7021 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7081 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7141 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7201 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7261 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7321 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7381 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7441 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7501 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7561 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7621 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7681 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7741 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7801 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7861 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7921 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7981 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8041 AACGCGAATT TT
//