LOCUS       RC230630                8049 bp    DNA     circular  SYN 
DEFINITION  MIB2 (Myc-DDK-tagged)-Human mindbomb homolog 2 (Drosophila) (MIB2), transcript variant 3
ACCESSION   NM_001170687
VERSION     NM_001170687.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4206..4297 
                     /label="Myc-DDK"  
     misc_feature    4327..4912 
                     /label="PolyA signal"  
     misc_feature    4384..4403 
                     /label="Restriction Enzyme"  
     misc_feature    5188..5860 
                     /label="ColE1"  
     misc_feature    6290..7084 
                     /label="Kan/Neo"  
     misc_feature    7119..7421 
                     /label="SV40 ori"  
     misc_feature    7507..7984 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4188..4194 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4188 
                     /label="MIB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGGGCG CTCCGGCGGG GGCGGGCCCT GGGCTCCCGC CCTTCGGGTC 
     1081 CCACAGTTTC CAGCCGCCGC TCTCCTCAGT GCCCGGTGGC CCAGGAGGGC CTGGGAGCCC 
     1141 GAAGCCGTCC CCGAGTCGCT CCTAGGTCAC TGGCGCGATG CGGGCCGTCC TCTCGGCTGA 
     1201 TGGGTTGGAA GCCCAGCGAG GCTAGAGGCC AGTCCCAAAG TTTCCAGGCA TCAGGGCTGC 
     1261 AGCCCAGGAG CCTCAAGGCG GCCCGGCGGG CGACTGGACG GCCGGACAGG TCCCGAGCAG 
     1321 CCCCGCCCAA CATGGACCCA GACCCCCAGG CGGGCGTGCA GGTGGGCATG CGGGTGGTGC 
     1381 GCGGCGTGGA CTGGAAGTGG GGCCAGCAGG ACGGCGGCGA GGGCGGCGTG GGCACGGTGG 
     1441 TGGAGCTTGG CCGCCACGGC AGCCCCTCGA CACCCGACCG CACAGTGGTC GTGCAGTGGG 
     1501 ACCAGGGCAC GCGCACCAAC TACCGCGCCG GCTACCAGGG CGCGCACGAC CTGCTGCTGT 
     1561 ACGACAACGC CCAGATCGGC GTCCGGCACC CCAACATCAT CTGTGACTGC TGCAAGAAGC 
     1621 ACGGGCTGCG GGGGATGCGC TGGAAGTGCC GTGTGTGCCT GGACTACGAC CTCTGCACGC 
     1681 AGTGCTACAT GCACAACAAG CATGAGCTCG CCCACGCCTT CGACCGCTAC GAGACCGCTC 
     1741 ACTCGCGCCC TGTCACACTG AGTCCCCGCC AGGGCCTCCC GAGGATCCCA CTAAGGGGCA 
     1801 TCTTCCAGGG AGCGAAGGTG GTGCGAGGCC CCGACTGGGA GTGGGGCTCA CAGGATGGAG 
     1861 GGGAAGGGAA ACCGGGCCGT GTGGTGGACA TCCGTGGCTG GGATGTGGAG ACAGGCCGGA 
     1921 GTGTGGCCAG CGTGACGTGG GCTGATGGTA CCACCAATGT GTACCGTGTG GGCCACAAGG 
     1981 GCAAGGTGGA CCTCAAGTGT GTGGGCGAGG CAGCGGGCGG CTTCTACTAC AAGGACCACC 
     2041 TCCCAAGGCT CGGCAAGCCG GCGGAGCTGC AGCGCAGGGT GAGTGCTGAC AGCCAGCCCT 
     2101 TCCAGCACGG GGACAAGGTC AAGTGTCTGC TGGACACTGA TGTCCTGCGG GAGATGCAGG 
     2161 AAGGCCACGG CGGCTGGAAC CCCAGGATGG CGGAGTTTAT CGGACAGACG GGCACCGTGC 
     2221 ATCGTATCAC GGACCGCGGG GACGTGCGCG TGCAGTTCAA CCACGAGACG CGCTGGACCT 
     2281 TCCACCCCGG GGCGCTCACC AAGCACCACT CCTTCTGGGT GGGCGACGTG GTCCGGGTCA 
     2341 TCGGCGACCT TGACACAGTG AAGCGGCTGC AGGCTGGGCA TGGCGAGTGG ACGGACGACA 
     2401 TGGCCCCTGC CCTGGGCCGC GTCGGGAAGG TGGTGAAAGT GTTTGGAGAC GGGAACCTGC 
     2461 GTGTAGCAGT CGCTGGTCAG CGGTGGACCT TCAGCCCCTC CTGCCTGGTG GCCTACCGGC 
     2521 CCGAGGAGGA TGCCAACCTG GACGTGGCCG AGCGCGCCCG GGAGAACAAA AGCTCACTGA 
     2581 GCGTGGCCCT GGACAAGCTT CGGGCCCAGA AGAGTGACCC AGAGCACCCG GGAAGGCTGG 
     2641 TGGTGGAGGT GGCGCTGGGT AACGCAGCCC GGGCTCTGGA CCTGCTGCGG AGGCGCCCAG 
     2701 AGCAGGTGGA CACCAAGAAC CAAGGCAGGA CCGCTCTGCA AGTGGCTGCC TACCTGGGCC 
     2761 AGGTGGAGTT GATACGGCTG CTGCTACAAG CCAGGGCGGG CGTGGACCTG CCGGACGACG 
     2821 AGGGCAACAC GGCACTGCAC TACGCGGCCC TGGGGAACCA GCCCGAGGCC ACCAGGGTGC 
     2881 TCCTGAGTGC TGGGTGCCGG GCGGACGCCA TCAACAGCAC CCAGAGCACA GCACTGCACG 
     2941 TGGCCGTGCA GAGGGGCTTC CTGGAGGTGG TGCGGGCCCT GTGTGAGCGC GGCTGTGACG 
     3001 TCAACCTGCC CGACGCCCAC TCGGACACGC CCCTGCACTC CGCCATCTCG GCGGGCACTG 
     3061 GAGCCAGCGG CATTGTCGAG GTCCTCACGG AGGTGCCAAA CATCGATGTT ACCGCCACCA 
     3121 ACAGCCAGGG TTTCACCCTG CTGCACCATG CCTCCCTCAA GGGTCACGCG CTAGCTGTGA 
     3181 GAAAGATTCT GGCTCGGGCG CGGCAGCTGG TGGACGCCAA GAAGGAGGAC GGCTTCACGG 
     3241 CGCTGCATCT GGCTGCCCTC AACAACCACC GCGAGGTGGC CCAGATCCTC ATCCGGGAGG 
     3301 GCCGCTGTGA CGTGAACGTG CGCAACCGGA AGCTGCAGTC CCCGCTGCAT CTCGCCGTGC 
     3361 AACAGGCCCA CGTGGGGCTG GTGCCGCTAC TGGTGGACGC TGGGTGCAGT GTCAACGCCG 
     3421 AGGACGAGGA GGGGGACACA GCCCTGCACG TGGCGCTGCA GCGTCATCAG CTGCTGCCCC 
     3481 TGGTGGCTGA TGGGGCCGGG GGGGACCCAG GGCCCTTGCA GCTGCTGTCC AGGCTACAGG 
     3541 CCTCGGGCCT CCCCGGCAGC GCGGAGCTGA CGGTGGGCGC GGCGGTCGCC TGCTTCCTGG 
     3601 CGCTGGAGGG CGCCGACGTG AGCTACACCA ACCACCGCGG TCGGAGCCCG CTGGACCTGG 
     3661 CCGCCGAGGG TCGCGTGCTC AAGGCCCTTC AGGGCTGCGC CCAGCGCTTC CGGGAGCGGC 
     3721 AGGCGGGCGG GGGCGCGGCC CCGGGCCCCA GGCAAACGCT CGGGACCCCC AACACCGTGA 
     3781 CGAACCTGCA CGTGGGCGCC GCGCCGGGGC CCGAGGCCGC TGAGTGCCTG GTGTGCTCCG 
     3841 AGCTGGCGCT GCTGGTGCTG TTCTCGCCGT GCCAGCACCG CACCGTGTGT GAGGAGTGCG 
     3901 CGCGCAGGAT GAAGAAGTGC ATCAGGTGCC AGGTGGTCGT CAGCAAGAAA CTGCGCCCAG 
     3961 ACGGCTCTGA GGTGGCGAGC GCCGCCCCCG CCCCCGGCCC GCCGCGCCAG CTGGTGGAGG 
     4021 AGCTGCAGAG CCGCTACCGG CAGATGGAGG AACGCATCAC CTGCCCCATC TGCATCGACA 
     4081 GCCACATCCG CCTCGTGTTC CAGTGCGGCC ACGGCGCATG CGCCCCCTGC GGCTCCGCGC 
     4141 TCAGCGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGACGC 
     4201 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4261 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4321 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4381 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4441 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4501 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4561 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4621 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4681 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4741 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4801 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4861 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4921 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4981 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5041 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5101 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5161 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5221 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5281 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5341 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5401 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5461 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5521 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5581 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5641 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5701 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5761 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5821 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5881 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5941 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6001 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6061 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6121 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6181 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6241 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6301 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6361 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6421 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6481 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6541 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6601 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6661 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6721 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6781 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6841 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6901 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6961 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7021 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7081 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7141 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7201 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7261 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7321 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7381 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7441 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7501 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7561 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7621 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7681 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7741 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7801 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7861 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7921 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7981 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8041 GCGAATTTT
//