LOCUS       RC230627                7998 bp    DNA     circular  SYN 
DEFINITION  NLRP2 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 2 (NLRP2), transcript variant 4
ACCESSION   NM_001174083
VERSION     NM_001174083.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4155..4246 
                     /label="Myc-DDK"  
     misc_feature    4276..4861 
                     /label="PolyA signal"  
     misc_feature    4333..4352 
                     /label="Restriction Enzyme"  
     misc_feature    5137..5809 
                     /label="ColE1"  
     misc_feature    6239..7033 
                     /label="Kan/Neo"  
     misc_feature    7068..7370 
                     /label="SV40 ori"  
     misc_feature    7456..7933 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4137..4143 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4137 
                     /label="NLRP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGTCTTCG GCGCAGATGG GCTTCAACCT GCAGGCTCTC CTGGAGCAGC 
     1081 TCAGCCAGGA TGAGTTGAGC AAGTTCAAGT ATCTGATCAC GACCTTCTCC CTGGCACACG 
     1141 AGCTCCAGAA GATCCCCCAC AAGGAGATGG CGAGCCTCCA GGTCTTTGAA AAGATGCACC 
     1201 GAATGGATCT GTCTGAGAGA GCAAAGGATG AAGTCAGAGA AGCAGCTTTG AAATCCTTTA 
     1261 ATAAAAGGAA GCCTCTATCA TTAGGGATAA CACGGAAAGA ACGACCACCT CTAGACGTGG 
     1321 ACGAAATGCT GGAGCGCTTC AAAACAGAAG CACAAGCGTT TACAGAAACG AAAGGAAATG 
     1381 TCATCTGCCT GGGTAAAGAA GTCTTTAAAG GAAAAAAGCC AGACAAAGAC AATAGGTGCA 
     1441 GGTATATATT GAAGACGAAG TTCCGGGAGA TGTGGAAGAG CTGGCCTGGA GATAGCAAAG 
     1501 AGGTCCAGGT TATGGCTGAG AGATACAAGA TGCTGATCCC ATTCAGCAAC CCCAGGGTGC 
     1561 TTCCCGGGCC CTTCTCATAC ACGGTGGTGC TGTATGGTCC TGCAGGCCTT GGGAAAACCA 
     1621 CGCTGGCCCA GAAACTAATG CTAGACTGGG CAGAGGACAA CCTCATCCAC AAATTCAAAT 
     1681 ATGCGTTCTA CCTCAGCTGC AGGGAGCTCA GCCGCCTGGG CCCGTGCAGT TTTGCAGAGC 
     1741 TGGTCTTCAG GGACTGGCCT GAATTGCAGG ATGACATTCC ACACATCCTA GCCCAAGCAC 
     1801 GGAAAATCTT GTTCGTGATT GACGGCTTTG ATGAGCTGGG AGCCGCACCT GGGGCGCTGA 
     1861 TCGAGGACAT CTGCGGGGAC TGGGAGAAGA AGAAGCCGGT GCCCGTCCTC CTGGGGAGTT 
     1921 TGCTGAACAG GGTGATGTTA CCCAAGGCCG CCCTGCTGGT CACCACGCGG CCCAGGGCCC 
     1981 TGAGGGACCT CCGGATCCTG GCGGAGGAGC CGATCTACAT AAGGGTGGAG GGCTTCCTGG 
     2041 AGGAGGACAG GAGGGCCTAT TTCCTGAGAC ACTTTGGAGA CGAGGACCAA GCCATGCGTG 
     2101 CCTTTGAGCT AATGAGGAGC AACGCGGCCC TGTTCCAGCT GGGCTCGGCC CCCGCGGTGT 
     2161 GCTGGATCGT GTGCACGACT CTGAAGCTGC AGATGGAGAA GGGGGAGGAC CCGGTCCCCA 
     2221 CCTGCCTCAC CCGCACGGGG CTGTTCCTGC GTTTCCTCTG CAGCCGGTTC CCGCAGGGCG 
     2281 CACAGCTGCG GGGCGCGCTG CGGACGCTGA GCCTCCTGGC CGCGCAGGGC CTGTGGGCGC 
     2341 AGACGTCCGT GCTTCACCGA GAGGATCTGG AAAGGCTCGG GGTGCAGGAG TCCGACCTCC 
     2401 GTCTGTTCCT GGACGGAGAC ATCCTCCGCC AGGACAGAGT CTCCAAAGGC TGCTACTCCT 
     2461 TCATCCACCT CAGCTTCCAG CAGTTTCTCA CTGCCCTGTT CTACACCCTG GAGAAGGAGG 
     2521 AGGAAGAGGA TAGGGACGGC CACACCTGGG ACATTGGGGA CGTACAGAAG CTGCTTTCCG 
     2581 GAGTAGAAAG ACTCAGGAAC CCCGACCTGA TCCAAGCAGG CTACTACTCC TTTGGCCTCG 
     2641 CTAACGAGAA GAGAGCCAAG GAGTTGGAGG CCACTTTTGG CTGCCGGATG TCACCGGACA 
     2701 TCAAACAGGA ATTGCTGCGA TGCGACATAA GTTGTAAGGG TGGACATTCA ACGGTGACAG 
     2761 ACCTGCAGGA GCTCCTCGGC TGTCTGTACG AGTCTCAGGA GGAGGAGCTG GTGAAGGAGG 
     2821 TGATGGCTCA GTTCAAAGAA ATATCCCTGC ACTTAAATGC AGTAGACGTT GTGCCATCTT 
     2881 CATTCTGCGT CAAGCACTGT CGAAACCTGC AGAAAATGTC ACTGCAGGTA ATAAAGGAGA 
     2941 ATCTCCCGGA GAATGTCACT GCGTCTGAAT CAGACGCCGA GGTTGAGAGA TCCCAGGATG 
     3001 ATCAGCACAT GCTTCCTTTC TGGACGGACC TTTGTTCCAT ATTTGGATCA AATAAGGATC 
     3061 TGATGGGTCT AGCAATCAAT GATAGCTTTC TCAGTGCCTC CCTAGTAAGG ATCCTGTGTG 
     3121 AACAAATAGC CTCTGACACC TGTCATCTCC AGAGAGTGGT GTTCAAAAAC ATTTCCCCAG 
     3181 CTGATGCTCA TCGGAACCTC TGCCTAGCTC TTCGAGGTCA CAAGACTGTA ACGTATCTGA 
     3241 CCCTTCAAGG CAATGACCAG GATGATATGT TTCCCGCATT GTGTGAGGTC TTGAGACATC 
     3301 CAGAATGTAA CCTGCGATAT CTCGGGTTGG TGTCTTGTTC CGCTACCACT CAGCAGTGGG 
     3361 CTGATCTCTC CTTGGCCCTT GAAGTCAACC AGTCCCTGAC GTGCGTAAAC CTCTCCGACA 
     3421 ATGAGCTTCT GGATGAGGGT GCTAAGTTGC TGTACACAAC TTTGAGACAC CCCAAGTGCT 
     3481 TTCTGCAGAG GTTGTCGTTG GAAAACTGTC ACCTTACAGA AGCCAATTGC AAGGACCTTG 
     3541 CTGCTGTGTT GGTTGTCAGC CGGGAGCTGA CACACCTGTG CTTGGCCAAG AACCCCATTG 
     3601 GGAATACAGG GGTGAAGTTT CTGTGTGAGG GCTTGAGGTA CCCCGAGTGT AAACTGCAGA 
     3661 CCTTGGTGCT TTGGAACTGC GACATAACTA GCGATGGCTG CTGCGATCTC ACAAAGCTTC 
     3721 TCCAAGAAAA ATCAAGCCTG TTGTGTTTGG ATCTGGGGCT GAATCACATA GGAGTTAAGG 
     3781 GAATGAAGTT CCTGTGTGAG GCTTTGAGGA AACCACTGTG CAACTTGAGA TGTCTGTGGT 
     3841 TGTGGGGATG TTCCATCCCT CCGTTCAGTT GTGAAGACCT CTGCTCTGCC CTCAGCTGCA 
     3901 ACCAGAGCCT CGTCACTCTG GACCTGGGTC AGAATCCCTT GGGGTCTAGT GGAGTGAAGA 
     3961 TGCTGTTTGA AACCTTGACA TGTTCCAGTG GCACCCTCCG GACACTCAGG TTGAAAATCG 
     4021 ATGACTTTAA TGATGAACTC AATAAGCTGC TGGAAGAAAT AGAAGAAAAA AACCCACAAC 
     4081 TGATTATTGA TACTGAGAAA CATCATCCCT GGGCAGAAAG GCCTTCTTCT CATGACTTCA 
     4141 TGATCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4201 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4261 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4321 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4381 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4441 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4501 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4561 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4621 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4681 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4741 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4801 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4861 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4921 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4981 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5041 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5101 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5161 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5221 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5281 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5341 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5401 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5461 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5521 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5581 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5641 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5701 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5761 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5821 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5881 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5941 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6001 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6061 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6121 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6181 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6241 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6301 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6361 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6421 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6481 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6541 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6601 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6661 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6721 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6781 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6841 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6901 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6961 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7021 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7081 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7141 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7201 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7261 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7321 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7381 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7441 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7501 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7561 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7621 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7681 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7741 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7801 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7861 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7921 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7981 AAATTTAACG CGAATTTT
//