LOCUS       RC230626                7983 bp    DNA     circular  SYN 
DEFINITION  CPEB2 (Myc-DDK-tagged)-Human cytoplasmic polyadenylation element binding protein 2 (CPEB2), transcript variant D. Note: ORF is codon optimized
ACCESSION   NM_001177382
VERSION     NM_001177382.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4140..4231 
                     /label="Myc-DDK"  
     misc_feature    4261..4846 
                     /label="PolyA signal"  
     misc_feature    4318..4337 
                     /label="Restriction Enzyme"  
     misc_feature    5122..5794 
                     /label="ColE1"  
     misc_feature    6224..7018 
                     /label="Kan/Neo"  
     misc_feature    7053..7355 
                     /label="SV40 ori"  
     misc_feature    7441..7918 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4122..4128 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4122 
                     /label="CPEB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGACTTC GGCTTTGGCG TCCTGCAAAC CGCTCCGCTC CGCTCTAGCT 
     1081 CCCCCGGCCC TCTGTTTTGC GGGGAGGCTT ATGGGCCCTA CGCCGTAGGT AGTGTGAATC 
     1141 CATTGCCCAG CGCAACACCT TTTGGACCGC TGAGCCCACC CCCTCTGCCA GTGACAGGTT 
     1201 TTCTGGAGGC AGCCTCCCCA TTCTCCGTCC CCCTTGGCGG AGGTGCTGGA TCCCCAGCCG 
     1261 CAGCAGCATC TTCCAGCAGT CCTTTTCTGG CCCACCAGCA GACAATGCAG GATGAGCTCC 
     1321 TTTTGGGTCT TACCCAGCAA CCCGCACGAC CATTGTCTGG GGCCGCCGCT ACAGAGAAGC 
     1381 TGCCCGATCA CCACCCCGGT GGTGGCACCA TTGCCGGGGT GACACATTTG CTGCCAAGTC 
     1441 AGGATTTTAA GCCCAGCCTG CACCACCCCT CATCAAGCAG TGCCAGCAGT TGCTGCTGTT 
     1501 GTAGGACTTC AAGCCCCCAG GACTTCAGTA AGAGACAGCA ACAGCAGCTT TCAAGCCAGA 
     1561 AGCGGAAGGA GTTTTCCCCG CCTCACCTGC CCCACCCCCC CGACAGTAAG CCACCCCCTC 
     1621 CACCCCCTCC ACTGCATTGT CCTGGGCGCT TCTCCCCTCC ACCCCCCCCA GCTGGACCCC 
     1681 TCCTGCAGCC TGCCCAGCTG GCACAGCGCC AGCAGCAGCA ACCCCCTCAA CAGTTCAGCT 
     1741 TGCTGCACCA GCAGCACCTG TCCCCTCAGG ACTTTGCCCC ACGCCAGCGG CCCGCTGATC 
     1801 TCCCTCCTCT CCCCCAGCTG CCACCATCAC CCCCAGCAGC ACCCAGAAGG CGCCATGGTG 
     1861 GTGCCGGATC TCCTCGAAAG ACGCCCGCCG CCGGAGAGGG ATCTGCCGCC GAGAGCCCCA 
     1921 ACGCAGGGTT GGCCTCTTCT ACACCGGTAA ATCCTGCTCC CGGCTCCATG GAATCCCCAA 
     1981 ACCATCCCCT CCTCAATTCC CCGAGTAATC TTCTGCCTGG GGGCGCCCTG GGCGCGGGAG 
     2041 CCTTTTCCTC TCTGCAGAGT CCTGACCTGC CGCATCCTGG CGGGGGGGGG GGAGGGGGGG 
     2101 GTGGGGGGCC ACCTGGGGGG GGAGGAGGTG GTGGTAGTGC CAGCCCTCCA CCTCTCCCAG 
     2161 GCTTCGGAAC GCCTTGGAGC GTGCAAACTG CCTCTCCTCC CCCACAGCCG CAGCAGCCAC 
     2221 CCCCTACCCA GCCTCAGCAA CAGCCACCCC CTCCCCAGCA GCCTCCTCAG CCGCAGCCTC 
     2281 AGCCCCCCGG TTCATCCGCT ACTACTCCAG GGGGCGGCAG CGGCGGCTCT CTCAGCGCAA 
     2341 TGCCCCCTCC TTCCCCCGAC TCAGAGAATG GATTCTATCC TGGACTCCCC AGCAGTATGA 
     2401 ATCCAGCATT TTTTCCATCT TTTAGTCCTG TTAGCCCCCA TGGCTGTACC GGCCTCAGTG 
     2461 TCCCAACCTC CGGCGGCGGA GGCGGGGGCT TCGGAGGCCC CTTTTCTGCC ACTGCAGTCC 
     2521 CTCCCCCTCC TCCCCCCGCG ATGAACATCC CCCAGCAACA GCCCCCACCA CCGGCCGCTC 
     2581 CCCAACAGCC TCAGTCTAGG CGGAGTCCCG TCTCACCGCA GCTGCAGCAG CAGCACCAAG 
     2641 CAGCCGCAGC CGCTTTCCTT CAGCAGCGGA ACAGTTACAA TCATCATCAG CCGCTCCTTA 
     2701 AACAGTCCCC TTGGAGTAAC CACCAATCCT CCGGTTGGGG GACGGGGTCT ATGAGTTGGG 
     2761 GGGCGATGCA CGGGAGAGAT CACAGAAGGA CAGGAAACAT GGGAATCCCT GGGACAATGA 
     2821 ACCAAATAAG TCCCCTCAAA AAGCCCTTCA GCGGAAATGT TATCGCACCC CCCAAATTCA 
     2881 CACGGTCCAC ACCTTCATTG ACACCGAAGT CCTGGATAGA AGACAATGTT TTTAGGACTG 
     2941 ACAACAATAG CAATACTCTG CTCCCACTGC AGGTGCGAAG CTCCCTTCAG CTGCCCGCCT 
     3001 GGGGATCAGA CAGCCTCCAA GACTCCTGGT GCACCGCAGC CGGCACAAGC CGAATCGATC 
     3061 AGGACCGCAG CCGGATGTAC GACTCTCTGA ATATGCACAG TCTGGAGAAC AGCCTGATTG 
     3121 ACATTATGCG AGCTGAGCAC GACCCCCTTA AGGGCCGCCT CTCATATCCA CATCCCGGAA 
     3181 CCGACAATCT TCTCATGCTT AACGCCCGCA GTTATGGAAG GCGCCGAGGC AGATCTTCCC 
     3241 TGTTCCCTAT TGATGACGGT CTTCTGGACG ACGGCCATTC AGATCAAGTA GGAGTGCTTA 
     3301 ACAGCCCCAC CTGCTACAGT GCACACCAGA ACGGCGAGAG AATTGAGAGG TTCTCTCGAA 
     3361 AAGTTTTTGT GGGTGGGCTT CCTCCCGACA TTGACGAGGA TGAGATTACA GCCAGTTTCA 
     3421 GGCGCTTTGG GCCCTTGGTC GTAGACTGGC CTCACAAAGC AGAATCCAAG AGCTACTTTC 
     3481 CTCCCAAAGG CTACGCCTTT CTCCTGTTCC AAGAAGAGTC CTCTGTCCAG GCGCTCATTG 
     3541 ATGCTTGCAT CGAAGAAGAC GGGAAGCTTT ATCTGTGCGT TTCTTCACCT ACTATAAAAG 
     3601 ATAAACCAGT GCAGATTCGA CCCTGGAATC TGTCCGACAG CGATTTCGTC ATGGACGGTA 
     3661 GCCAGCCACT TGACCCAAGA AAGACCATCT TTGTGGGCGG TGTCCCTCGC CCACTGCGAG 
     3721 CCGTTGAGCT CGCCATGATC ATGGATCGAC TCTACGGTGG AGTATGCTAC GCCGGAATCG 
     3781 ATACAGATCC GGAGCTCAAA TACCCAAAGG GAGCTGGAAG GGTGGCCTTC TCCAATCAGC 
     3841 AATCATATAT CGCTGCGATT TCCGCTCGAT TCGTGCAGTT GCAACATGGC GATATCGACA 
     3901 AAAGAGTGGA AGTAAAACCG TATGTGCTTG ATGACCAAAT GTGTGACGAG TGCCAGGGCG 
     3961 CTCGGTGTGG GGGTAAGTTT GCCCCCTTTT TCTGTGCCAA TGTAACTTGC TTGCAGTATT 
     4021 ACTGCGAATT CTGCTGGGCA AACATTCACT CTCGAGCCGG GAGGGAGTTC CACAAACCCT 
     4081 TGGTCAAGGA GGGAGCTGAC CGCCCACGCC AAATCCACTT CCGATGGAAC ACGCGTACGC 
     4141 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4201 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4261 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4321 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4381 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4441 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4501 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4561 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4621 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4681 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4741 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4801 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4861 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4921 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4981 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5041 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5101 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5161 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5221 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5281 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5341 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5401 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5461 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5521 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5581 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5641 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5701 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5761 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5821 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5881 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5941 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6001 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6061 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6121 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6181 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6241 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6301 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6361 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6421 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6481 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6541 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6601 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6661 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6721 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6781 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6841 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6901 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6961 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7021 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7081 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7141 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7201 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7261 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7321 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7381 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7441 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7501 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7561 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7621 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7681 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7741 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7801 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7861 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7921 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7981 TTT
//