LOCUS       RC230625                7974 bp    DNA     circular  SYN 
DEFINITION  PARD3 (Myc-DDK-tagged)-Human par-3 partitioning defective 3 homolog (C. elegans) (PARD3), transcript variant 9
ACCESSION   NM_001184792
VERSION     NM_001184792.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4131..4222 
                     /label="Myc-DDK"  
     misc_feature    4252..4837 
                     /label="PolyA signal"  
     misc_feature    4309..4328 
                     /label="Restriction Enzyme"  
     misc_feature    5113..5785 
                     /label="ColE1"  
     misc_feature    6215..7009 
                     /label="Kan/Neo"  
     misc_feature    7044..7346 
                     /label="SV40 ori"  
     misc_feature    7432..7909 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4113..4119 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4113 
                     /label="PARD3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGACC GTGTGCTTCG GACGGACCCG GGTGGTCGTG CCGTGCGGGG 
     1081 ACGGCCACAT GAAAGTTTTC AGCCTCATCC AGCAGGCGGT GACCCGCTAC CGGAAGGCCA 
     1141 TCGCCAAGGA TCCAAACTAC TGGATACAGG TGCATCGCTT GGAACATGGA GATGGAGGAA 
     1201 TACTAGACCT TGATGACATT CTTTGTGATG TAGCAGACGA TAAAGACAGA CTGGTAGCAG 
     1261 TGTTTGATGA GCAGGATCCA CATCACGGAG GTGATGGCAC CAGTGCCAGT TCCACGGGTA 
     1321 CCCAGAGCCC AGAGATATTT GGTAGTGAGC TTGGCACCAA CAATGTCTCA GCCTTTCAGC 
     1381 CTTACCAAGC AACAAGTGAA ATTGAGGTCA CACCTTCAGT CCTTCGAGCA AATATGCCTC 
     1441 TTCATGTTCG ACGCAGTAGT GACCCAGCTC TAATTGGCCT CTCCACTTCT GTCAGTGATA 
     1501 GTAATTTTTC CTCTGAAGAG CCTTCAAGGA AAAATCCCAC ACGCTGGTCA ACAACAGCTG 
     1561 GCTTCCTCAA GCAGAACACT GCTGGGAGTC CTAAAACCTG CGACAGGAAG AAAGATGAAA 
     1621 ACTACAGAAG CCTCCCGCGG GATACTAGTA ACTGGTCTAA CCAATTTCAG AGAGACAATG 
     1681 CTCGCTCGTC TCTGAGTGCC AGTCACCCAA TGGTGGGCAA GTGGCTGGAG AAACAAGAAC 
     1741 AGGATGAGGA TGGGACAGAA GAGGATAACA GTCGTGTTGA ACCTGTTGGA CATGCTGACA 
     1801 CGGGTTTGGA GCATATACCC AACTTTTCTC TGGATGATAT GGTAAAGCTC GTAGAAGTCC 
     1861 CCAACGATGG AGGGCCTCTG GGAATCCATG TAGTGCCTTT CAGTGCTCGA GGCGGCAGAA 
     1921 CCCTGGGGTT ATTAGTAAAA CGATTGGAGA AAGGTGGTAA AGCTGAACAT GAAAATCTTT 
     1981 TTCGTGAGAA TGATTGCATT GTCAGGATTA ATGATGGCGA CCTTCGAAAT AGAAGATTTG 
     2041 AACAAGCACA ACATATGTTT CGCCAAGCCA TGCGTACACC CATCATTTGG TTCCATGTGG 
     2101 TTCCTGCAGC AAATAAAGAG CAGTATGAAC AACTATCCCA AAGTGAGAAG AACAATTACT 
     2161 ATTCAAGCCG TTTTAGCCCT GACAGCCAGT ATATTGACAA CAGGAGTGTG AACAGTGCAG 
     2221 GGCTTCACAC GGTGCAGAGA GCACCCCGAC TGAACCACCC GCCTGAGCAG ATAGACTCTC 
     2281 ACTCAAGACT ACCTCATAGC GCACACCCCT CGGGAAAACC ACCATCCGCT CCAGCCTCGG 
     2341 CACCTCAGAA TGTATTTAGT ACGACTGTAA GCAGTGGTTA TAACACCAAA AAAATAGGCA 
     2401 AGAGGCTTAA TATCCAGCTT AAGAAAGGTA CAGAAGGTTT GGGATTCAGC ATCACTTCCA 
     2461 GAGATGTAAC AATAGGTGGC TCAGCTCCAA TCTATGTGAA AAACATTCTC CCCCGGGGGG 
     2521 CGGCCATTCA GGATGGCCGA CTTAAGGCAG GAGACAGACT TATAGAGGTA AATGGAGTAG 
     2581 ATTTAGTGGG CAAATCCCAA GAGGAAGTTG TTTCGCTGTT GAGAAGCACC AAGATGGAAG 
     2641 GAACTGTGAG CCTTCTGGTC TTTCGCCAGG AAGACGCCTT CCACCCAAGG GAACTGAATG 
     2701 CAGAGCCAAG CCAGATGCAG ATTCCAAAAG AAACGAAAGC AGAAGATGAG GATATTGTTC 
     2761 TTACACCTGA TGGCACCAGG GAATTTCTGA CATTTGAAGT CCCACTTAAT GATTCAGGAT 
     2821 CTGCAGGCCT TGGTGTCAGT GTCAAAGGTA ACCGGTCAAA AGAGAACCAC GCAGATTTGG 
     2881 GAATCTTTGT CAAGTCCATT ATTAATGGAG GAGCAGCATC TAAAGATGGA AGGCTTCGGG 
     2941 TGAATGATCA ACTGATAGCA GTAAATGGAG AATCCCTGTT GGGCAAGACA AACCAAGATG 
     3001 CCATGGAAAC CCTAAGAAGG TCTATGTCTA CTGAAGGCAA TAAACGAGGA ATGATCCAGC 
     3061 TTATTGTTGC AAGGAGAATA AGCAAGTGCA ATGAGCTGAA GTCACCTGGG AGCCCCCCTG 
     3121 GACCTGAGCT GCCCATTGAA ACAGCGTTGG ATGATAGAGA ACGAAGAATT TCCCATTCCC 
     3181 TCTACAGTGG GATTGAGGGG CTTGATGAAT CGCCCAGCAG AAATGCTGCC CTCAGTAGGA 
     3241 TAATGGGTAA ATACCAGCTG TCCCCTACAG TGAATATGCC CCAAGATGAC ACTGTCATTA 
     3301 TAGAAGATGA CAGGTTGCCA GTGCTTCCTC CACATCTCTC TGACCAGTCC TCTTCCAGCT 
     3361 CCCATGATGA TGTGGGGTTT GTGACGGCAG ATGCTGGTAC TTGGGCCAAG GCTGCAATCA 
     3421 GTGATTCAGC CGACTGCTCT TTGAGTCCAG ATGTTGATCC AGTTCTTGCT TTTCAACGAG 
     3481 AAGGATTTGG ACGTCAGAGT ATGTCAGAAA AACGCACAAA GCAATTTTCA GATGCCAGTC 
     3541 AATTGGATTT CGTTAAAACA CGAAAATCAA AAAGCATGGA TTTAGGTATA GCTGACGAGA 
     3601 CTAAACTCAA TACAGTGGAT GACCAGAAAG CAGGTTCTCC CAGCAGAGAT GTGGGTCCTT 
     3661 CCCTGGGTCT GAAGAAGTCA AGCTCGTTGG AGAGTCTGCA GACCGCAGTT GCCGAGGTGA 
     3721 CTTTGAATGG GGATATTCCT TTCCATCGTC CACGGCCGCG GATAATCAGA GGCAGGGGAT 
     3781 GCAATGAGAG CTTCAGAGCT GCCATCGACA AATCTTATGA TAAACCCGCG GTAGATGATG 
     3841 ATGATGAAGG CATGGAGACC TTGGAAGAAG ACACAGAAGA AAGTTCAAGA TCAGGGAGAG 
     3901 AGTCTGTATC CACAGCCAGT GATCAGCCTT CCCACTCTCT GGAGAGACAA ATGAATGGAA 
     3961 ACCAAGAGAA AGGTGATAAG ACTGATAGAA AAAAGGATAA AACTGGAAAA GAAAAGAAGA 
     4021 AAGATAGAGA TAAGGAGAAG GATAAAATGA AAGCCAAGAA GGGAATGCTG AAGGGCTTGG 
     4081 GAGACATGTT CAGCCTTGCC AAACTGAAGC CCGAGAAGAG AACGCGTACG CGGCCGCTCG 
     4141 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4201 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4261 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4321 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4381 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4441 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4501 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4561 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4621 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4681 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4741 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4801 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4861 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4921 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4981 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5041 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5101 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5161 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5221 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5281 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5341 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5401 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5461 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5521 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5581 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5641 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5701 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5761 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5821 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5881 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5941 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6001 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6061 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6121 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6181 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6241 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6301 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6361 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6421 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6481 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6541 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6601 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6661 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6721 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6781 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6841 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6901 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6961 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7021 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7081 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7141 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7201 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7261 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7321 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7381 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7441 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7501 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7561 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7621 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7681 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7741 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7801 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7861 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7921 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//