LOCUS       RC230624                7962 bp    DNA     circular  SYN 
DEFINITION  KIAA0319 (Myc-DDK-tagged)-Human KIAA0319 (KIAA0319), transcript variant 4
ACCESSION   NM_001168376
VERSION     NM_001168376.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4119..4210 
                     /label="Myc-DDK"  
     misc_feature    4240..4825 
                     /label="PolyA signal"  
     misc_feature    4297..4316 
                     /label="Restriction Enzyme"  
     misc_feature    5101..5773 
                     /label="ColE1"  
     misc_feature    6203..6997 
                     /label="Kan/Neo"  
     misc_feature    7032..7334 
                     /label="SV40 ori"  
     misc_feature    7420..7897 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4101..4107 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4101 
                     /label="KIAA0319"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGTGTCT CACACCTTCC CTGTCGTAGA CTGCACGGCC GCTTGCTGTG 
     1081 ACCTGTCCAG CTGTGACCTG GCCTGGTGGT TCGAGGGCCG CTGCTACCTG GTGAGCTGCC 
     1141 CCCACAAAGA GAACTGTGAG CCCAAGAAGA TGGGCCCCAT CAGGTCTTAT CTCACTTTTG 
     1201 TGCTCCGGCC TGTTCAGAGG CCTGCACAGC TGCTGGACTA TGGGGACATG ATGCTGAACA 
     1261 GGGGCTCCCC CTCGGGGATC TGGGGGGACT CACCTGAGGA TATCAGAAAG GACTTGACCT 
     1321 TTCTAGGCAA AGATTGGGGC CTAGAGGAGA TGTCTGAGTA CTCAGATGAC TACCGGGAGC 
     1381 TGGAGAAGGA CCTCTTGCAA CCCAGTGGCA AGCAGGAGCC CAGAGGGAGT GCCGAGTACA 
     1441 CGGACTGGGG CCTACTGCCG GGCAGCGAGG GGGCCTTCAA CTCCTCTGTT GGAGACAGTC 
     1501 CTGCGGTGCC AGCGGAGACG CAGCAGGACC CTGAGCTCCA TTACCTGAAT GAGTCGGCTT 
     1561 CAACCCCTGC CCCAAAACTC CCTGAGAGAA GTGTGTTGCT TCCCTTGCCG ACTACTCCAT 
     1621 CTTCAGGAGA GGTGTTGGAG AAAGAAAAGG CTTCTCAGCT CCAGGAACAA TCCAGCAACA 
     1681 GCTCTGGAAA AGAGGTTCTA ATGCCTTCCC ATAGTCTTCC TCCGGCAAGC CTGGAGCTCA 
     1741 GCTCAGTCAC CGTGGAGAAA AGCCCAGTGC TCACAGTCAC CCCGGGGAGT ACAGAGCACA 
     1801 GCATCCCAAC ACCTCCCACT AGCGCAGCCC CCTCTGAGTC CACCCCATCT GAGCTACCCA 
     1861 TATCTCCTAC CACTGCTCCC AGGACAGTGA AAGAACTTAC GGTATCGGCT GGAGATAACC 
     1921 TAATTATAAC TTTACCCGAC AATGAAGTTG AACTGAAGGC CTTTGTTGCG CCAGCGCCAC 
     1981 CTGTAGAAAC AACCTACAAC TATGAATGGA ATTTAATAAG CCACCCCACA GACTACCAAG 
     2041 GTGAAATAAA ACAAGGACAC AAGCAAACTC TTAACCTCTC TCAATTGTCC GTCGGACTTT 
     2101 ATGTCTTCAA AGTCACTGTT TCTAGTGAAA ACGCCTTTGG AGAAGGATTT GTCAATGTCA 
     2161 CTGTTAAGCC TGCCAGAAGA GTCAACCTGC CACCTGTAGC AGTTGTTTCT CCCCAACTGC 
     2221 AAGAGCTCAC TTTGCCTTTG ACGTCAGCCC TCATTGATGG CAGCCAAAGT ACAGATGATA 
     2281 CTGAAATAGT GAGTTATCAT TGGGAAGAAA TAAACGGGCC CTTCATAGAA GAGAAGACTT 
     2341 CAGTTGACTC TCCCGTCTTA CGCTTGTCTA ACCTTGATCC TGGTAACTAT AGTTTCAGGT 
     2401 TGACTGTTAC AGACTCGGAC GGAGCCACTA ACTCTACAAC TGCAGCCCTA ATAGTGAACA 
     2461 ATGCTGTGGA CTACCCACCA GTTGCTAATG CAGGACCAAA TCACACCATA ACTTTGCCCC 
     2521 AAAACTCCAT CACTTTGAAT GGAAACCAGA GCAGTGACGA TCACCAGATT GTCCTCTATG 
     2581 AGTGGTCCCT GGGTCCTGGG AGTGAGGGCA AACATGTGGT CATGCAGGGA GTACAGACGC 
     2641 CATACCTTCA TTTATCTGCA ATGCAGGAAG GAGATTATAC ATTTCAGCTG AAGGTGACAG 
     2701 ATTCTTCAAG GCAACAGTCT ACTGCTGTGG TGACTGTGAT TGTCCAGCCT GAAAACAATA 
     2761 GACCTCCAGT GGCTGTGGCC GGCCCTGATA AAGAGCTGAT CTTCCCAGTG GAAAGTGCTA 
     2821 CCCTGGATGG GAGCAGCAGC AGCGATGACC ACGGCATTGT CTTCTACCAC TGGGAGCACG 
     2881 TCAGAGGCCC CAGTGCAGTG GAGATGGAAA ATATTGACAA AGCAATAGCC ACTGTGACTG 
     2941 GTCTCCAGGT GGGGACCTAC CACTTCCGTT TGACAGTGAA AGACCAGCAG GGACTGAGCA 
     3001 GCACGTCCAC CCTCACTGTG GCTGTGAAGA AGGAAAATAA TAGTCCTCCC AGAGCCCGGG 
     3061 CTGGTGGCAG ACATGTTCTT GTGCTTCCCA ATAATTCCAT TACTTTGGAT GGTTCAAGGT 
     3121 CTACTGATGA CCAAAGAATT GTGTCCTATC TGTGGATCCG GGATGGCCAG AGTCCAGCAG 
     3181 CTGGAGATGT CATCGATGGC TCTGACCACA GTGTGGCTCT GCAGCTTACG AATCTGGTGG 
     3241 AGGGGGTGTA CACTTTCCAC TTGCGAGTCA CCGACAGTCA GGGGGCCTCG GACACAGACA 
     3301 CTGCCACTGT GGAAGTGCAG CCAGACCCTA GGAAGAGTGG CCTGGTGGAG CTGACCCTGC 
     3361 AGGTTGGTGT TGGGCAGCTG ACAGAGCAGC GGAAGGACAC CCTTGTGAGG CAGCTGGCTG 
     3421 TGCTGCTGAA CGTGCTGGAC TCGGACATTA AGGTCCAGAA GATTCGGGCC CACTCGGATC 
     3481 TCAGCACCGT GATTGTGTTT TATGTACAGA GCAGGCCGCC TTTCAAGGTT CTCAAAGCTG 
     3541 CTGAAGTGGC CCGAAATCTG CACATGCGGC TCTCAAAGGA GAAGGCTGAC TTCTTGCTTT 
     3601 TCAAGGTCTT GAGGGTTGAT ACAGCAGGTT GCCTTCTGAA GTGTTCTGGC CATGGTCACT 
     3661 GCGACCCCCT CACAAAGCGC TGCATTTGCT CTCACTTATG GATGGAGAAC CTTATACAGC 
     3721 GTTATATCTG GGATGGAGAG AGCAACTGTG AGTGGAGTAT ATTCTATGTG ACAGTGTTGG 
     3781 CTTTTACTCT TATTGTGCTA ACAGGAGGTT TCACTTGGCT TTGCATCTGC TGCTGCAAAA 
     3841 GACAAAAAAG GACTAAAATC AGGAAAAAAA CAAAGTACAC CATCCTGGAT AACATGGATG 
     3901 AACAGGAAAG AATGGAACTG AGGCCCAAAT ATGGTATCAA GCACCGAAGC ACAGAGCACA 
     3961 ACTCCAGCCT GATGGTATCC GAGTCTGAGT TTGACAGTGA CCAGGACACA ATCTTCAGCC 
     4021 GAGAAAAGAT GGAGAGAGGG AATCCAAAGG TTTCCATGAA TGGTTCCATC AGAAATGGAG 
     4081 CTTCCTTCAG TTATTGCTCA AAGGACAGAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4141 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4201 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4261 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4321 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4381 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4441 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4501 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4561 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4621 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4681 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4741 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4801 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4861 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4921 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4981 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5041 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5101 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5161 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5221 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5281 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5341 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5401 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5461 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5521 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5581 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5641 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5701 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5761 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5821 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5881 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5941 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6001 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6061 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6121 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6181 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6241 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6301 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6361 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6421 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6481 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6541 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6601 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6661 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6721 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6781 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6841 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6901 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6961 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7021 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7081 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7141 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7201 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7261 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7321 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7381 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7441 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7501 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7561 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7621 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7681 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7741 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7801 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7861 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7921 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//