LOCUS       RC230621                7941 bp    DNA     circular  SYN 
DEFINITION  TJP2 (Myc-DDK-tagged)-Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 5
ACCESSION   NM_001170414
VERSION     NM_001170414.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4098..4189 
                     /label="Myc-DDK"  
     misc_feature    4219..4804 
                     /label="PolyA signal"  
     misc_feature    4276..4295 
                     /label="Restriction Enzyme"  
     misc_feature    5080..5752 
                     /label="ColE1"  
     misc_feature    6182..6976 
                     /label="Kan/Neo"  
     misc_feature    7011..7313 
                     /label="SV40 ori"  
     misc_feature    7399..7876 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4080..4086 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4080 
                     /label="TJP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGAGCTG ATATGGGAAC AGTACACTGT GACCCTACAA AAGGATTCCA 
     1081 AAAGAGGATT TGGAATTGCA GTGTCCGGAG GCAGAGACAA CCCCCACTTT GAAAATGGAG 
     1141 AAACGTCAAT TGTCATTTCT GATGTGCTCC CGGGTGGGCC TGCTGATGGG CTGCTCCAAG 
     1201 AAAATGACAG AGTGGTCATG GTCAATGGCA CCCCCATGGA GGATGTGCTT CATTCGTTTG 
     1261 CAGTTCAGCA GCTCAGAAAA AGTGGGAAGG TCGCTGCTAT TGTGGTCAAG AGGCCCCGGA 
     1321 AGGTCCAGGT GGCCGCACTT CAGGCCAGCC CTCCCCTGGA TCAGGATGAC CGGGCTTTTG 
     1381 AGGTGATGGA CGAGTTTGAT GGCAGAAGTT TCCGGAGTGG CTACAGCGAG AGGAGCCGGC 
     1441 TGAACAGCCA TGGGGGGCGC AGCCGCAGCT GGGAGGACAG CCCGGAAAGG GGGCGTCCCC 
     1501 ATGAGCGGGC CCGGAGCCGG GAGCGGGACC TCAGCCGGGA CCGGAGCCGT GGCCGGAGCC 
     1561 TGGAGCGGGG CCTGGACCAA GACCATGCGC GCACCCGAGA CCGCAGCCGT GGCCGGAGCC 
     1621 TGGAGCGGGG CCTGGACCAC GACTTTGGGC CATCCCGGGA CCGGGACCGT GACCGCAGCC 
     1681 GCGGCCGGAG CATTGACCAG GACTACGAGC GAGCCTATCA CCGGGCCTAC GACCCAGACT 
     1741 ACGAGCGGGC CTACAGCCCG GAGTACAGGC GCGGGGCCCG CCACGATGCC CGCTCTCGGG 
     1801 GACCCCGAAG CCGCAGCCGC GAGCACCCGC ACTCACGGAG CCCCAGCCCC GAGCCTAGGG 
     1861 GGCGGCCGGG GCCCATCGGG GTCCTCCTGA TGAAAAGCAG AGCGAACGAA GAGTATGGTC 
     1921 TCCGGCTTGG GAGTCAGATC TTCGTAAAGG AAATGACCCG AACGGGTCTG GCAACTAAAG 
     1981 ATGGCAACCT TCACGAAGGA GACATAATTC TCAAGATCAA TGGGACTGTA ACTGAGAACA 
     2041 TGTCTTTAAC GGATGCTCGA AAATTGATAG AAAAGTCAAG AGGAAAACTA CAGCTAGTGG 
     2101 TGTTGAGAGA CAGCCAGCAG ACCCTCATCA ACATCCCGTC ATTAAATGAC AGTGACTCAG 
     2161 AAATAGAAGA TATTTCAGAA ATAGAGTCAA ACCGATCATT TTCTCCAGAG GAGAGACGTC 
     2221 ATCAGTATTC TGATTATGAT TATCATTCCT CAAGTGAGAA GCTGAAGGAA AGGCCAAGTT 
     2281 CCAGAGAGGA CACGCCGAGC AGATTGTCCA GGATGGGTGC GACACCCACT CCCTTTAAGT 
     2341 CCACAGGGGA TATTGCAGGC ACAGTTGTCC CAGAGACCAA CAAGGAACCC AGATACCAAG 
     2401 AGGACCCCCC AGCTCCTCAA CCAAAAGCAG CCCCGAGAAC TTTTCTTCGT CCTAGTCCTG 
     2461 AAGATGAAGC AATATATGGC CCTAATACCA AAATGGTAAG GTTCAAGAAG GGAGACAGCG 
     2521 TGGGCCTCCG GTTGGCTGGT GGCAATGATG TCGGGATATT TGTTGCTGGC ATTCAAGAAG 
     2581 GGACCTCGGC GGAGCAGGAG GGCCTTCAAG AAGGAGACCA GATTCTGAAG GTGAACACAC 
     2641 AGGATTTCAG AGGATTAGTG CGGGAGGATG CCGTTCTCTA CCTGTTAGAA ATCCCTAAAG 
     2701 GTGAAATGGT GACCATTTTA GCTCAGAGCC GAGCCGATGT GTATAGAGAC ATCCTGGCTT 
     2761 GTGGCAGAGG GGATTCGTTT TTTATAAGAA GCCACTTTGA ATGTGAGAAG GAAACTCCAC 
     2821 AGAGCCTGGC CTTCACCAGA GGGGAGGTCT TCCGAGTGGT AGACACACTG TATGACGGCA 
     2881 AGCTGGGCAA CTGGCTGGCT GTGAGGATTG GGAACGAGTT GGAGAAAGGC TTAATCCCCA 
     2941 ACAAGAGCAG AGCTGAACAA ATGGCCAGTG TTCAAAATGC CCAGAGAGAC AACGCTGGGG 
     3001 ACCGGGCAGA TTTCTGGAGA ATGCGTGGCC AGAGGTCTGG GGTGAAGAAG AACCTGAGGA 
     3061 AAAGTCGGGA AGACCTCACA GCTGTTGTGT CTGTCAGCAC CAAGTTCCCA GCTTATGAGA 
     3121 GGGTTTTGCT GCGAGAAGCT GGTTTCAAGA GACCTGTGGT CTTATTCGGC CCCATAGCTG 
     3181 ATATAGCAAT GGAAAAATTG GCTAATGAGT TACCTGACTG GTTTCAAACT GCTAAAACGG 
     3241 AACCAAAAGA TGCAGGATCT GAGAAATCCA CTGGAGTGGT CCGGTTAAAT ACCGTGAGGC 
     3301 AAATTATTGA ACAGGATAAG CATGCACTAC TGGATGTGAC TCCGAAAGCT GTGGACCTGT 
     3361 TGAATTACAC CCAGTGGTTC CCAATTGTGA TTTTTTTCAA CCCAGACTCC AGACAAGGTG 
     3421 TCAAAACCAT GAGACAAAGG TTAAATCCAA CGTCCAACAA AAGTTCTCGA AAGTTATTTG 
     3481 ATCAAGCCAA CAAGCTTAAA AAAACGTGTG CACACCTTTT TACAGCTACA ATCAACCTAA 
     3541 ATTCAGCCAA TGATAGCTGG TTTGGCAGCT TAAAGGACAC TATTCAGCAT CAGCAAGGAG 
     3601 AAGCGGTTTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGACCCC GAAGACCGCA 
     3661 TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG CGACAGCCGC CTCATCAGTG 
     3721 ACTTTGAAGA CACGGACGGT GAAGGAGGCG CCTACACTGA CAATGAGCTG GATGAGCCAG 
     3781 CCGAGGAGCC GCTGGTGTCG TCCATCACCC GCTCCTCGGA GCCGGTGCAG CACGAGGAGA 
     3841 TCGAAATTGC CCAGAAGCAT CCTGATATCT ATGCAGTTCC AATCAAAACG CACAAGCCAG 
     3901 ACCCTGGCAC GCCCCAGCAC ACGAGTTCCA GACCCCCTGA GCCACAGAAA GCTCCTTCCA 
     3961 GACCTTATCA GGATACCAGA GGAAGTTATG GCAGTGATGC CGAGGAGGAG GAGTACCGCC 
     4021 AGCAGCTGTC AGAACACTCC AAGCGCGGTT ACTATGGCCA GTCTGCCCGA TACCGGGACA 
     4081 CAGAATTAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4141 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4201 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4261 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4321 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4381 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4441 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4501 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4561 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4621 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4681 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4741 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4801 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4861 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4921 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4981 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5041 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5101 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5161 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5221 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5281 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5341 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5401 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5461 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5521 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5581 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5641 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5701 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5761 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5821 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5881 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5941 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6001 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6061 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6121 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6181 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6241 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6301 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6361 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6421 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6481 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6541 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6601 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6661 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6721 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6781 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6841 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6901 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6961 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7021 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7081 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7141 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7201 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7261 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7321 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7381 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7441 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7501 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7561 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7621 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7681 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7741 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7801 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7861 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7921 CAAAAATTTA ACGCGAATTT T
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