LOCUS       RC230614                7896 bp    DNA     circular  SYN 
DEFINITION  MIB2 (Myc-DDK-tagged)-Human mindbomb homolog 2 (Drosophila) (MIB2), transcript variant 4
ACCESSION   NM_001170688
VERSION     NM_001170688.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4053..4144 
                     /label="Myc-DDK"  
     misc_feature    4174..4759 
                     /label="PolyA signal"  
     misc_feature    4231..4250 
                     /label="Restriction Enzyme"  
     misc_feature    5035..5707 
                     /label="ColE1"  
     misc_feature    6137..6931 
                     /label="Kan/Neo"  
     misc_feature    6966..7268 
                     /label="SV40 ori"  
     misc_feature    7354..7831 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4035..4041 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4035 
                     /label="MIB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGGGCG CTCCGGCGGG GGCGGGCCCT GGGCTCCCGC CCTTCGGGTC 
     1081 CCACAGTTTC CAGCCGCCGC TCTCCTCAGT GCCCGGTGGC CCAGGAGGGC CTGGGAGCCC 
     1141 GAAGCCGTCC CCGAGTCGCT CCTAGGTCAC TGGCGCGATG CGGGCCGTCC TCTCGGCTGA 
     1201 TGGGTTGGAA GCCCAGCGAG GCTAGAGGCC AGTCCCAAAG TTTCCAGGCA TCAGGGCTGC 
     1261 AGCCCAGGAG CCTCAAGGCG GCCCGGCGGG CGACTGGACG GCCGGACAGG TCCCGAGCAG 
     1321 CCCGGCCCAC CATGGACCCC TCTGCCCACA GGTCCCGAGC AGCCCCGCCC AACATGGACC 
     1381 CAGACCCCCA GGCGGGCGTG CAGGTGGGCA TGCGGGTGGT GCGCGGCGTG GACTGGAAGT 
     1441 GGGGCCAGCA GGACGGCGGC GAGGGCGGCG TGGGCACGGT GGTGGAGCTT GGCCGCCACG 
     1501 GCAGCCCCTC GACACCCGAC CGCACAGTGG TCGTGCAGTG GGACCAGGGC ACGCGCACCA 
     1561 ACTACCGCGC CGGCTACCAG GGCGCGCACG ACCTGCTGCT GTACGACAAC GCCCAGATCG 
     1621 GCGTCCGGCA CCCCAACATC ATCTGTGACT GCTGCAAGAA GCACGGGCTG CGGGGGATGC 
     1681 GCTGGAAGTG CCGTGTGTGC CTGGACTACG ACCTCTGCAC GCAGTGCTAC ATGCACAACA 
     1741 AGCATGAGCT CGCCCACGCC TTCGACCGCT ACGAGACCGC TCACTCGCGC CCTGTCACAC 
     1801 TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CACTAAGGGG CATCTTCCAG GGAGCGAAGG 
     1861 TGGTGCGAGG CCCCGACTGG GAGTGGGGCT CACAGGATGG CAAGCCGGCG GAGCTGCAGC 
     1921 GCAGGGTGAG TGCTGACAGC CAGCCCTTCC AGCACGGGGA CAAGGTCAAG TGTCTGCTGG 
     1981 ACACTGATGT CCTGCGGGAG ATGCAGGAAG GCCACGGCGG CTGGAACCCC AGGATGGCGG 
     2041 AGTTTATCGG ACAGACGGGC ACCGTGCATC GTATCACGGA CCGCGGGGAC GTGCGCGTGC 
     2101 AGTTCAACCA CGAGACGCGC TGGACCTTCC ACCCCGGGGC GCTCACCAAG CACCACTCCT 
     2161 TCTGGGTGGG CGACGTGGTC CGGGTCATCG GCGACCTTGA CACAGTGAAG CGGCTGCAGG 
     2221 CTGGGCATGG CGAGTGGACG GACGACATGG CCCCTGCCCT GGGCCGCGTC GGGAAGGTGG 
     2281 TGAAAGTGTT TGGAGACGGG AACCTGCGTG TAGCAGTCGC TGGTCAGCGG TGGACCTTCA 
     2341 GCCCCTCCTG CCTGGTGGCC TACCGGCCCG AGGAGGATGC CAACCTGGAC GTGGCCGAGC 
     2401 GCGCCCGGGA GAACAAAAGC TCACTGAGCG TGGCCCTGGA CAAGCTTCGG GCCCAGAAGA 
     2461 GTGACCCAGA GCACCCGGGA AGGCTGGTGG TGGAGGTGGC GCTGGGTAAC GCAGCCCGGG 
     2521 CTCTGGACCT GCTGCGGAGG CGCCCAGAGC AGGTGGACAC CAAGAACCAA GGCAGGACCG 
     2581 CTCTGCAAGT GGCTGCCTAC CTGGGCCAGG TGGAGTTGAT ACGGCTGCTG CTACAAGCCA 
     2641 GGGCGGGCGT GGACCTGCCG GACGACGAGG GCAACACGGC ACTGCACTAC GCGGCCCTGG 
     2701 GGAACCAGCC CGAGGCCACC AGGGTGCTCC TGAGTGCTGG GTGCCGGGCG GACGCCATCA 
     2761 ACAGCACCCA GAGCACAGCA CTGCACGTGG CCGTGCAGAG GGGCTTCCTG GAGGTGGTGC 
     2821 GGGCCCTGTG TGAGCGCGGC TGTGACGTCA ACCTGCCCGA CGCCCACTCG GACACGCCCC 
     2881 TGCACTCCGC CATCTCGGCG GGCACTGGAG CCAGCGGCAT TGTCGAGGTC CTCACGGAGG 
     2941 TGCCAAACAT CGATGTTACC GCCACCAACA GCCAGGGTTT CACCCTGCTG CACCATGCCT 
     3001 CCCTCAAGGG TCACGCGCTA GCTGTGAGAA AGATTCTGGC TCGGGCGCGG CAGCTGGTGG 
     3061 ACGCCAAGAA GGAGGACGGC TTCACGGCGC TGCATCTGGC TGCCCTCAAC AACCACCGCG 
     3121 AGGTGGCCCA GATCCTCATC CGGGAGGGCC GCTGTGACGT GAACGTGCGC AACCGGAAGC 
     3181 TGCAGTCCCC GCTGCATCTC GCCGTGCAAC AGGCCCACGT GGGGCTGGTG CCGCTACTGG 
     3241 TGGACGCTGG GTGCAGTGTC AACGCCGAGG ACGAGGAGGG GGACACAGCC CTGCACGTGG 
     3301 CGCTGCAGCG TCATCAGCTG CTGCCCCTGG TGGCTGATGG GGCCGGGGGG GACCCAGGGC 
     3361 CCTTGCAGCT GCTGTCCAGG CTACAGGCCT CGGGCCTCCC CGGCAGCGCG GAGCTGACGG 
     3421 TGGGCGCGGC GGTCGCCTGC TTCCTGGCGC TGGAGGGCGC CGACGTGAGC TACACCAACC 
     3481 ACCGCGGTCG GAGCCCGCTG GACCTGGCCG CCGAGGGTCG CGTGCTCAAG GCCCTTCAGG 
     3541 GCTGCGCCCA GCGCTTCCGG GAGCGGCAGG CGGGCGGGGG CGCGGCCCCG GGCCCCAGGC 
     3601 AAACGCTCGG GACCCCCAAC ACCGTGACGA ACCTGCACGT GGGCGCCGCG CCGGGGCCCG 
     3661 AGGCCGCTGA GTGCCTGGTG TGCTCCGAGC TGGCGCTGCT GGTGCTGTTC TCGCCGTGCC 
     3721 AGCACCGCAC CGTGTGTGAG GAGTGCGCGC GCAGGATGAA GAAGTGCATC AGGTGCCAGG 
     3781 TGGTCGTCAG CAAGAAACTG CGCCCAGACG GCTCTGAGGT GGCGAGCGCC GCCCCCGCCC 
     3841 CCGGCCCGCC GCGCCAGCTG GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAAC 
     3901 GCATCACCTG CCCCATCTGC ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG 
     3961 GCGCATGCGC CCCCTGCGGC TCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC 
     4021 GCGACCGCAT CCAGATCTTC GTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4081 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4141 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4201 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4261 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4321 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4381 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4441 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4501 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4561 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4621 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4681 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4741 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4801 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4861 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4921 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4981 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5041 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5101 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5161 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5221 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5281 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5341 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5401 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5461 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5521 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5581 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5641 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5701 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5761 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5821 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5881 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5941 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6001 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6061 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6121 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6181 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6241 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6301 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6361 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6421 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6481 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6541 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6601 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6661 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6721 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6781 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6841 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6901 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6961 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7021 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7081 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7141 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7201 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7261 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7321 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7381 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7441 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7501 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7561 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7621 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7681 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7741 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7801 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7861 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//