LOCUS       RC230541                7434 bp    DNA     circular  SYN 
DEFINITION  PFKM (Myc-DDK-tagged)-Human phosphofructokinase, muscle (PFKM), transcript variant 1
ACCESSION   NM_001166686
VERSION     NM_001166686.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3591..3682 
                     /label="Myc-DDK"  
     misc_feature    3712..4297 
                     /label="PolyA signal"  
     misc_feature    3769..3788 
                     /label="Restriction Enzyme"  
     misc_feature    4573..5245 
                     /label="ColE1"  
     misc_feature    5675..6469 
                     /label="Kan/Neo"  
     misc_feature    6504..6806 
                     /label="SV40 ori"  
     misc_feature    6892..7369 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3573..3579 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3573 
                     /label="PFKM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATAAAGAC GAGTTTCATC TGAAATTTTT CATGTGTGTG ATTCAGTCTC 
     1081 GCCAGTTAGT CAGGACTCCT CAGAGAACAG CTGGGGAAGC TTCTACTTCC AGCATGCTCA 
     1141 TACCAAAGCC ACCACCAAAG ACAGACATCT TGAAGAGTCT AGATACTATG GATGATCCAG 
     1201 ACACCGTGGG AAGCATACCT GTTTTCAAAA CTGAGTGGAT CATGACCCAT GAAGAGCACC 
     1261 ATGCAGCCAA AACCCTGGGG ATTGGCAAAG CCATTGCTGT CTTAACCTCT GGTGGAGATG 
     1321 CCCAAGGTAT GAATGCTGCT GTCAGGGCTG TGGTTCGAGT TGGTATCTTC ACCGGTGCCC 
     1381 GTGTCTTCTT TGTCCATGAG GGTTATCAAG GCCTGGTGGA TGGTGGAGAT CACATCAAGG 
     1441 AAGCCACCTG GGAGAGCGTT TCGATGATGC TTCAGCTGGG AGGCACGGTG ATTGGAAGTG 
     1501 CCCGGTGCAA GGACTTTCGG GAACGAGAAG GACGACTCCG AGCTGCCTAC AACCTGGTGA 
     1561 AGCGTGGGAT CACCAATCTC TGTGTCATTG GGGGTGATGG CAGCCTCACT GGGGCTGACA 
     1621 CCTTCCGTTC TGAGTGGAGT GACTTGTTGA GTGACCTCCA GAAAGCAGGT AAGATCACAG 
     1681 ATGAGGAGGC TACGAAGTCC AGCTACCTGA ACATTGTGGG CCTGGTTGGG TCAATTGACA 
     1741 ATGACTTCTG TGGCACCGAT ATGACCATTG GCACTGACTC TGCCCTGCAT CGGATCATGG 
     1801 AAATTGTAGA TGCCATCACT ACCACTGCCC AGAGCCACCA GAGGACATTT GTGTTAGAAG 
     1861 TAATGGGCCG CCACTGTGGA TACCTGGCCC TTGTCACCTC TCTGTCCTGT GGGGCCGACT 
     1921 GGGTTTTTAT TCCTGAATGT CCACCAGATG ACGACTGGGA GGAACACCTT TGTCGCCGAC 
     1981 TCAGCGAGAC AAGGACCCGT GGTTCTCGTC TCAACATCAT CATTGTGGCT GAGGGTGCAA 
     2041 TTGACAAGAA TGGAAAACCA ATCACCTCAG AAGACATCAA GAATCTGGTG GTTAAGCGTC 
     2101 TGGGATATGA CACCCGGGTT ACTGTCTTGG GGCATGTGCA GAGGGGTGGG ACGCCATCAG 
     2161 CCTTTGACAG AATTCTGGGC AGCAGGATGG GTGTGGAAGC AGTGATGGCA CTTTTGGAGG 
     2221 GGACCCCAGA TACCCCAGCC TGTGTAGTGA GCCTCTCTGG TAACCAGGCT GTGCGCCTGC 
     2281 CCCTCATGGA ATGTGTCCAG GTGACCAAAG ATGTGACCAA GGCCATGGAT GAGAAGAAAT 
     2341 TTGACGAAGC CCTGAAGCTG AGAGGCCGGA GCTTCATGAA CAACTGGGAG GTGTACAAGC 
     2401 TTCTAGCTCA TGTCAGACCC CCGGTATCTA AGAGTGGTTC GCACACAGTG GCTGTGATGA 
     2461 ACGTGGGGGC TCCGGCTGCA GGCATGAATG CTGCTGTTCG CTCCACTGTG AGGATTGGCC 
     2521 TTATCCAGGG CAACCGAGTG CTCGTTGTCC ATGATGGTTT CGAGGGCCTG GCCAAGGGGC 
     2581 AGATAGAGGA AGCTGGCTGG AGCTATGTTG GGGGCTGGAC TGGCCAAGGT GGCTCTAAAC 
     2641 TTGGGACTAA AAGGACTCTA CCCAAGAAGA GCTTTGAACA GATCAGTGCC AATATAACTA 
     2701 AGTTTAACAT TCAGGGCCTT GTCATCATTG GGGGCTTTGA GGCTTACACA GGGGGCCTGG 
     2761 AACTGATGGA GGGCAGGAAG CAGTTTGATG AGCTCTGCAT CCCATTTGTG GTCATTCCTG 
     2821 CTACAGTCTC CAACAATGTC CCTGGCTCAG ACTTCAGCGT TGGGGCTGAC ACAGCACTCA 
     2881 ATACTATCTG CACAACCTGT GACCGCATCA AGCAGTCAGC AGCTGGCACC AAGCGTCGGG 
     2941 TGTTTATCAT TGAGACTATG GGTGGCTACT GTGGCTACCT GGCTACCATG GCTGGACTGG 
     3001 CAGCTGGGGC CGATGCTGCC TACATTTTTG AGGAGCCCTT CACCATTCGA GACCTGCAGG 
     3061 CAAATGTTGA ACATCTGGTG CAAAAGATGA AAACAACTGT GAAAAGGGGC TTGGTGTTAA 
     3121 GGAATGAAAA GTGCAATGAG AACTATACCA CTGACTTCAT TTTCAACCTG TACTCTGAGG 
     3181 AGGGGAAGGG CATCTTCGAC AGCAGGAAGA ATGTGCTTGG TCACATGCAG CAGGGTGGGA 
     3241 GCCCAACCCC ATTTGATAGG AATTTTGCCA CTAAGATGGG CGCCAAGGCT ATGAACTGGA 
     3301 TGTCTGGGAA AATCAAAGAG AGTTACCGTA ATGGGCGGAT CTTTGCCAAT ACTCCAGATT 
     3361 CGGGCTGTGT TCTGGGGATG CGTAAGAGGG CTCTGGTCTT CCAACCAGTG GCTGAGCTGA 
     3421 AGGACCAGAC AGATTTTGAG CATCGAATCC CCAAGGAACA GTGGTGGCTG AAACTGAGGC 
     3481 CCATCCTCAA AATCCTAGCC AAGTACGAGA TTGACTTGGA CACTTCAGAC CATGCCCACC 
     3541 TGGAGCACAT CACCCGGAAG CGGTCCGGGG AAGCTGCCGT CACGCGTACG CGGCCGCTCG 
     3601 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3661 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3721 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3781 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3841 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3901 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3961 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4021 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4081 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4141 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4201 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4261 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4321 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4381 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4441 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4501 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4561 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4621 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4681 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4741 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4801 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4861 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4921 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4981 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5041 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5101 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5161 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5221 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5281 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5341 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5401 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5461 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5521 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5581 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5641 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5701 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5761 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5821 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5881 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5941 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6001 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6061 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6121 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6181 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6241 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6301 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6361 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6421 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6481 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6541 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6601 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6661 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6721 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6781 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6841 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6901 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6961 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7021 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7081 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7141 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7201 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7261 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7321 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7381 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//