LOCUS       RC230506                7305 bp    DNA     circular  SYN 
DEFINITION  NLGN3 (Myc-DDK-tagged)-Human neuroligin 3 (NLGN3), transcript variant 3
ACCESSION   NM_001166660
VERSION     NM_001166660.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3462..3553 
                     /label="Myc-DDK"  
     misc_feature    3583..4168 
                     /label="PolyA signal"  
     misc_feature    3640..3659 
                     /label="Restriction Enzyme"  
     misc_feature    4444..5116 
                     /label="ColE1"  
     misc_feature    5546..6340 
                     /label="Kan/Neo"  
     misc_feature    6375..6677 
                     /label="SV40 ori"  
     misc_feature    6763..7240 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3444..3450 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3444 
                     /label="NLGN3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCTGCGG CTTGGCCCGC CCTCGCTGTC CCTGAGCCCC AAGCCCACGG 
     1081 TTGGCAGGAG CCTGTGCCTC ACCCTGTGGT TCCTCAGTTT GGCGCTGAGG GCCAGTACCC 
     1141 AGGCCCCAGC ACCCACAGTC AACACTCACT TTGGGAAGCT AAGGGGTGCC CGAGTACCAC 
     1201 TGCCCAGTGA GATCCTGGGG CCTGTGGACC AATACCTGGG GGTGCCCTAC GCAGCTCCCC 
     1261 CGATCGGCGA GAAACGTTTC CTGCCCCCTG AACCACCCCC ATCCTGGTCG GGCATCCGGA 
     1321 ACGCCACACA CTTTCCCCCA GTGTGCCCCC AGAACATCCA CACAGCTGTG CCCGAAGTCA 
     1381 TGCTGCCGGT CTGGTTCACT GCCAACTTGG ATATCGTCGC TACTTACATC CAGGAGCCCA 
     1441 ACGAAGACTG TCTCTACCTG AACGTCTATG TGCCGACGGA GGATGACATC CGGGACAGTG 
     1501 GTGCTAAACC CGTCATGGTC TACATCCACG GAGGCTCTTA CATGGAAGGG ACAGGCAACA 
     1561 TGATTGATGG CAGCATCCTC GCCAGTTATG GCAATGTCAT CGTCATCACC CTCAACTATC 
     1621 GGGTTGGAGT GCTAGGTTTC CTGAGTACTG GAGATCAGGC TGCCAAGGGC AACTATGGGC 
     1681 TCCTTGACCA GATCCAGGCC CTCCGCTGGG TGAGCGAGAA TATTGCCTTC TTCGGGGGAG 
     1741 ACCCCCGCCG GATCACTGTC TTTGGCTCGG GCATTGGTGC ATCCTGCGTC AGCCTCCTCA 
     1801 CGTTGTCACA TCACTCAGAG GGACTTTTCC AGAGAGCCAT CATCCAAAGT GGCTCTGCTC 
     1861 TGTCCAGCTG GGCTGTGAAC TACCAACCAG TGAAGTACAC CAGCCTGCTG GCAGACAAAG 
     1921 TGGGCTGTAA TGTGCTGGAC ACCGTGGATA TGGTGGACTG TCTTCGGCAA AAGAGTGCCA 
     1981 AGGAGCTGGT AGAGCAGGAC ATCCAGCCAG CCCGCTACCA CGTGGCCTTT GGCCCTGTGA 
     2041 TTGATGGTGA TGTCATTCCT GATGACCCTG AGATCCTCAT GGAGCAGGGC GAGTTCCTCA 
     2101 ACTATGACAT CATGCTAGGT GTCAACCAGG GCGAGGGTCT CAAGTTTGTG GAAGGGGTGG 
     2161 TGGACCCTGA GGATGGTGTC TCTGGCACTG ACTTTGACTA TTCCGTCTCC AATTTTGTGG 
     2221 ACAATCTGTA TGGCTATCCT GAGGGTAAGG ACACCCTGCG AGAGACCATC AAGTTCATGT 
     2281 ATACAGACTG GGCAGACCGT GACAACCCTG AGACCCGCCG TAAAACACTG GTGGCACTCT 
     2341 TCACTGACCA CCAGTGGGTG GAGCCCTCAG TGGTGACAGC CGATCTGCAT GCCCGCTACG 
     2401 GCTCGCCTAC CTACTTCTAC GCCTTCTATC ATCACTGCCA GAGCCTCATG AAGCCTGCTT 
     2461 GGTCAGATGC AGCTCATGGG GATGAAGTAC CCTATGTTTT TGGGGTTCCT ATGGTAGGCC 
     2521 CCACTGACCT TTTCCCCTGC AACTTCTCCA AGAATGATGT TATGCTCAGT GCTGTCGTCA 
     2581 TGACCTATTG GACCAACTTT GCCAAGACTG GGGATCCCAA CAAGCCGGTC CCCCAGGACA 
     2641 CCAAGTTCAT TCACACCAAG GCCAACCGCT TTGAGGAAGT GGCCTGGTCC AAATACAATC 
     2701 CCCGAGACCA GCTCTACCTT CACATCGGGC TGAAACCAAG GGTCCGAGAT CATTACCGGG 
     2761 CCACTAAGGT GGCCTTTTGG AAACATCTGG TGCCCCACCT ATACAACCTG CATGACATGT 
     2821 TCCACTATAC GTCCACCACC ACCAAAGTGC CGCCTCCGGA TACCACCCAC AGCTCCCACA 
     2881 TCACCCGCAG GCCCAATGGC AAGACCTGGA GCACCAAGCG GCCAGCCATC TCACCTGCCT 
     2941 ACAGCAACGA GAATGCCCAG GGGTCCTGGA ACGGGGACCA GGATGCAGGG CCACTCCTGG 
     3001 TGGAGAACCC TCGTGACTAC TCCACTGAAT TAAGTGTCAC CATCGCCGTG GGGGCCTCCC 
     3061 TCCTGTTCCT TAACGTTCTG GCCTTCGCTG CCCTCTACTA CCGTAAGGAC AAACGGCGCC 
     3121 AGGAGCCCCT GCGGCAGCCT AGCCCTCAGC GGGGAGCCGG GGCCCCGGAG TTGGGAGCTG 
     3181 CTCCAGAGGA GGAGCTGGCA GCATTACAAC TGGGCCCCAC CCACCACGAG TGTGAGGCCG 
     3241 GTCCCCCCCA TGACACGCTG CGCCTCACTG CATTGCCCGA CTACACCCTG ACCCTGCGGC 
     3301 GCTCCCCGGA TGACATCCCA CTCATGACCC CCAACACCAT CACTATGATC CCCAACTCCC 
     3361 TGGTAGGGCT GCAGACATTG CACCCCTATA ACACCTTTGC CGCAGGGTTC AACAGTACCG 
     3421 GGCTGCCCCA CTCACACTCC ACTACCCGGG TAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3481 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3541 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3601 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3661 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3721 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3781 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3841 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3901 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3961 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4021 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4081 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4141 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4201 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4261 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4321 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4381 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4441 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4501 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4561 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4621 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4681 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4741 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4801 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4861 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4921 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4981 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5041 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5101 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5161 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5221 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5281 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5341 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5401 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5461 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5521 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5581 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5641 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5701 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5761 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5821 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5881 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5941 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6001 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6061 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6121 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6181 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6241 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6301 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6361 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6421 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6481 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6541 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6601 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6661 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6721 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6781 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6841 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6901 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6961 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7021 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7081 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7141 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7201 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7261 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//