LOCUS       RC230490                7215 bp    DNA     circular  SYN 
DEFINITION  SP3 (Myc-DDK-tagged)-Human Sp3 transcription factor (SP3), transcript variant 3
ACCESSION   NM_001172712
VERSION     NM_001172712.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3372..3463 
                     /label="Myc-DDK"  
     misc_feature    3493..4078 
                     /label="PolyA signal"  
     misc_feature    3550..3569 
                     /label="Restriction Enzyme"  
     misc_feature    4354..5026 
                     /label="ColE1"  
     misc_feature    5456..6250 
                     /label="Kan/Neo"  
     misc_feature    6285..6587 
                     /label="SV40 ori"  
     misc_feature    6673..7150 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3354..3360 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3354 
                     /label="SP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGCTCCC GAAAAGCCCG TGAAACAAGA GGAAATGGCT GCCTTGGACG 
     1081 TGGATAGCGG CGGCGGCGGT GGCGGCGGCG GCGGCCACGG CGAGTATCTG CAGCAGCAGC 
     1141 AACAGCACGG AAACGGTGCG GTGGCGGCGG CAGCGGCGGC CCAGCCGTCA CCGCTCGCTC 
     1201 TGCTGGCCGC TACCTGCAGC AAGATAGGGC CGCCATCGCC GGGCGACGAC GAGGAGGAGG 
     1261 CGGCCGCCGC AGCCGGGGCC CCCGCCGCCG CCGGAGCGAC AGGTGATTTG GCTTCTGCAC 
     1321 AGTTAGGAGG AGCACCAAAC CGATGGGAGG TTTTGTCAGC CACACCTACA ACTATAAAAG 
     1381 ATGAAGCTGG TAATCTAGTC CAGATTCCAA GTGCTGCTAC TTCAAGTGGG CAGTATGTTC 
     1441 TTCCCCTTCA GAATTTGCAG AATCAACAAA TATTTTCCGT TGCACCAGGA TCAGATTCAT 
     1501 CAAATGGTAC AGTGTCCAGT GTTCAATATC AAGTGATACC ACAGATCCAG TCAGCAGATG 
     1561 GTCAGCAGGT TCAAATTGGT TTCACAGGCT CTTCAGATAA TGGGGGTATA AATCAAGAAA 
     1621 GCAGTCAAAT TCAGATCATT CCTGGCTCTA ATCAAACCTT ACTTGCCTCT GGAACACCTT 
     1681 CTGCTAACAT CCAGAATCTC ATACCACAGA CTGGTCAAGT CCAGGTTCAG GGAGTTGCAA 
     1741 TTGGTGGTTC ATCTTTTCCT GGTCAAACCC AAGTAGTTGC TAATGTGCCT CTTGGTCTGC 
     1801 CAGGAAATAT TACGTTTGTA CCAATCAATA GTGTCGATCT AGATTCTTTG GGACTCTCGG 
     1861 GCAGTTCTCA GACAATGACT GCAGGCATTA ATGCCGACGG ACATTTGATA AACACAGGAC 
     1921 AAGCTATGGA TAGTTCAGAC AATTCAGAAA GGACTGGTGA GCGGGTTTCT CCTGATATTA 
     1981 ATGAAACTAA TACTGATACA GATTTATTTG TGCCAACATC CTCTTCATCA CAGTTGCCTG 
     2041 TTACGATAGA TAGTACAGGT ATATTACAAC AAAACACAAA TAGCTTGACT ACATCTAGTG 
     2101 GGCAGGTTCA TTCTTCAGAT CTTCAGGGAA ATTATATCCA GTCGCCTGTT TCTGAAGAGA 
     2161 CACAGGCACA GAATATTCAG GTTTCTACAG CACAGCCTGT TGTACAGCAT CTACAACTTC 
     2221 AAGAGTCTCA GCAGCCAACC AGTCAAGCCC AAATTGTGCA AGGTATTACA CCACAGACAA 
     2281 TCCATGGTGT GCAAGCCAGT GGTCAAAATA TATCACAACA GGCTTTGCAA AATCTTCAGT 
     2341 TGCAGCTGAA TCCTGGAACC TTTTTAATTC AGGCACAGAC AGTGACCCCT TCTGGACAGG 
     2401 TAACTTGGCA AACGTTTCAA GTACAAGGGG TCCAGAACTT GCAGAATTTG CAAATACAGA 
     2461 ATACTGCTGC CCAACAAATA ACTTTGACGC CTGTTCAAAC CCTCACACTT GGTCAAGTTG 
     2521 CGGCAGGTGG AGCCTTCACT TCAACTCCAG TTAGTCTAAG CACTGGTCAG TTGCCAAATC 
     2581 TACAAACAGT TACAGTGAAC TCTATAGATT CTGCTGGTAT ACAGCTACAT CCAGGAGAGA 
     2641 ATGCTGACAG TCCTGCAGAT ATTAGGATCA AGGAAGAAGA ACCTGATCCT GAAGAGTGGC 
     2701 AGCTCAGTGG TGATTCTACC TTGAATACCA ATGACCTAAC ACACTTAAGA GTACAGGTGG 
     2761 TAGATGAAGA AGGGGACCAA CAACATCAAG AAGGAAAAAG ACTTCGGAGG GTAGCTTGCA 
     2821 CCTGTCCCAA CTGTAAAGAA GGTGGTGGAA GAGGTACCAA TCTTGGGAAA AAGAAGCAAC 
     2881 ACATTTGTCA TATACCAGGA TGTGGTAAAG TCTATGGGAA GACCTCACAT CTGAGAGCTC 
     2941 ATCTGCGTTG GCATTCTGGA GAACGCCCTT TTGTTTGTAA CTGGATGTAC TGTGGTAAAA 
     3001 GATTTACTCG AAGTGATGAA TTACAGAGGC ACAGAAGAAC ACATACAGGT GAGAAGAAAT 
     3061 TTGTTTGTCC AGAATGTTCA AAACGCTTTA TGAGAAGTGA CCACCTTGCC AAACATATTA 
     3121 AAACACACCA GAATAAAAAA GGTATTCACT CTAGCAGTAC AGTGCTGGCA TCTGTGGAAG 
     3181 CTGCGCGAGA TGATACTTTG ATTACTGCAG GAGGAACAAC GCTTATCCTT GCAAATATTC 
     3241 AACAAGGTTC TGTTTCAGGG ATAGGAACTG TTAATACTTC CGCCACCAGC AATCAAGATA 
     3301 TCCTTACCAA CACTGAAATA CCTTTACAGC TTGTCACAGT TTCTGGAAAT GAGACAATGG 
     3361 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3421 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3481 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3541 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3601 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3661 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3721 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3781 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3841 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3901 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3961 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4021 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4081 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4141 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4201 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4261 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4321 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4381 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4441 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4501 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4561 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4621 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4681 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4741 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4801 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4861 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4921 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     4981 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5041 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5101 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5161 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5221 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5281 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5341 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5401 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5461 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5521 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5581 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5641 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5701 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5761 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5821 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5881 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     5941 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6001 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6061 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6121 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6181 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6241 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6301 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6361 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6421 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6481 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6541 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6601 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6661 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6721 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6781 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6841 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6901 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     6961 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7021 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7081 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7141 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7201 TTTAACGCGA ATTTT
//