LOCUS       RC230458                7059 bp    DNA     circular  SYN 
DEFINITION  ADD2 (Myc-DDK-tagged)-Human adducin 2 (beta) (ADD2), transcript variant 5
ACCESSION   NM_001185054
VERSION     NM_001185054.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3216..3307 
                     /label="Myc-DDK"  
     misc_feature    3337..3922 
                     /label="PolyA signal"  
     misc_feature    3394..3413 
                     /label="Restriction Enzyme"  
     misc_feature    4198..4870 
                     /label="ColE1"  
     misc_feature    5300..6094 
                     /label="Kan/Neo"  
     misc_feature    6129..6431 
                     /label="SV40 ori"  
     misc_feature    6517..6994 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3198..3204 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3198 
                     /label="ADD2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGAAGAG ACGGTCCCCG AGGCTGCCTC GCCGCCGCCC CCGCAGGGGC 
     1081 AGCCTTACTT TGACCGCTTC TCAGAGGACG ACCCCGAGTA CATGCGCCTT CGCAACCGGG 
     1141 CGGCGGACCT GCGGCAGGAC TTCAACCTGA TGGAGCAGAA GAAGCGCGTC ACCATGATCC 
     1201 TGCAGAGTCC CTCTTTCAGG GAGGAGCTGG AAGGCCTCAT CCAGGAGCAG ATGAAGAAGG 
     1261 GGAACAACTC CTCCAACATC TGGGCCCTGC GACAGATCGC GGACTTCATG GCCAGCACCT 
     1321 CCCACGCAGT CTTCCCGACA TCTTCCATGA ATGTCTCCAT GATGACGCCT ATCAATGACC 
     1381 TCCACACAGC TGACTCCCTG AACCTGGCCA AAGGGGAGCG GCTCATGCGG TGCAAGATCA 
     1441 GCAGTGTCTA CCGACTCCTG GACCTCTATG GCTGGGCCCA GCTGAGTGAC ACCTATGTCA 
     1501 CGTTGAGAGT CAGCAAGGAG CAGGACCACT TCCTGATCAG CCCTAAGGGA GTTTCTTGCA 
     1561 GTGAAGTCAC AGCGTCCAGC CTGATCAAGG TGAACATTCT GGGAGAGGTG GTGGAGAAGG 
     1621 GCAGCAGCTG CTTCCCAGTG GACACCACAG GCTTCTGTCT GCACTCGGCC ATCTATGCAG 
     1681 CGAGGCCCGA CGTGCGCTGC ATCATCCACC TGCACACACC GGCCACAGCA GCGGTGTCGG 
     1741 CCATGAAGTG GGGCCTCCTG CCTGTCTCCC ACAATGCCCT GCTGGTGGGG GACATGGCCT 
     1801 ATTATGACTT CAATGGGGAA ATGGAGCAGG AAGCCGATCG GATCAACCTG CAGAAGTGCC 
     1861 TTGGACCCAC CTGCAAGATC CTGGTGCTAA GAAACCATGG AGTGGTTGCT CTGGGTGACA 
     1921 CGGTAGAGGA GGCATTTTAC AAGATCTTCC ACCTGCAGGC TGCATGTGAG ATACAGGTGT 
     1981 CGGCTCTGTC CAGTGCCGGG GGAGTGGAGA ACCTCATCCT CCTGGAGCAG GAGAAGCACC 
     2041 GGCCCCATGA GGTGGGCTCC GTGCAGTGGG CCGGGAGCAC CTTTGGGCCT ATGCAGAAGA 
     2101 GTCGGCTGGG GGAGCATGAG TTTGAGGCCC TCATGAGGAT GCTGGACAAC CTGGGCTACA 
     2161 GAACAGGTTA CACGTATCGC CACCCCTTTG TTCAAGAGAA AACCAAACAC AAAAGTGAGG 
     2221 TGGAGATTCC AGCCACGGTC ACAGCCTTCG TGTTTGAGGA GGACGGTGCC CCGGTGCCCG 
     2281 CCCTGCGACA GCATGCCCAG AAGCAGCAGA AGGAGAAGAC CCGCTGGCTC AATACGCCCA 
     2341 ACGCCTACCT GCGGGTCAAT GTGGCCGATG AGGTCCAGAG GAGCATGGGC AGCCCCCGAC 
     2401 CCAAGACCAC GTGGATGAAG GCTGACGAGG TGGAGAAATC CAGCAGTGGC ATGCCGATTC 
     2461 GCATCGAAAA CCCAAACCAA TTTGTGCCTC TCTATACTGA CCCCCAGGAA GTACTGGAGA 
     2521 TGAGGAACAA GATTCGAGAA CAAAACCGAC AAGATGTGAA GTCAGCGGGG CCTCAGTCCC 
     2581 AGCTCCTGGC GAGCGTCATT GCCGAGAAGA GCCGAAGCCC GTCTACAGAG AGCCAGCTGA 
     2641 TGTCCAAGGG AGACGAGGAT ACCAAAGACG ATTCAGAGGA GACGGTGCCC AACCCCTTCA 
     2701 GCCAACTCAC TGACCAGGAG TTGGAGGAGT ACAAGAAAGA GGTGGAGAGG AAGAAACTAG 
     2761 AACTTGATGG AGAGAAAGAA ACTGCCCCAG AAGAGCCTGG CTCACCTGCA AAGTCTGCAC 
     2821 CTGCTTCTCC AGTGCAGAGC CCAGCGAAGG AGGCAGAGAC AAAGAGCCCT TTAGTCTCTC 
     2881 CTTCCAAGTC TTTAGAGGAA GGTACTAAGA AGACAGAAAC AAGCAAAGCC GCCACCACAG 
     2941 AGCCCGAAAC AACCCAGCCG GAAGGGGTGG TGGTCAACGG GAGGGAGGAG GAGCAGACGG 
     3001 CAGAGGAAAT CCTCAGCAAA GGCCTGAGCC AGATGACCAC CAGTGCTGAC ACGGATGTTG 
     3061 ATACCTCTAA GGACAAAACC GAGTCGGTCA CCAGCGGCCC CATGTCCCCA GAGGGCTCAC 
     3121 CTTCCAAGTC TCCCTCAAAG AAGAAAAAGA AATTCCGAAC CCCCTCCTTC CTGAAAAAGA 
     3181 GCAAAAAGAA GGAGAAAGTG GAGTCCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3241 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3301 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3361 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3421 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3481 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3541 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3601 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3661 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3721 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3781 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3841 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     3901 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     3961 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4021 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4081 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4141 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4201 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4261 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4321 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4381 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4441 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4501 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4561 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4621 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4681 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4741 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4801 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4861 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     4921 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     4981 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5041 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5101 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5161 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5221 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5281 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5341 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5401 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5461 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5521 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5581 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5641 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5701 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5761 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5821 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     5881 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     5941 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6001 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6061 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6121 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6181 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6241 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6301 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6361 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6421 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6481 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6541 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6601 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6661 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6721 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6781 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6841 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     6901 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     6961 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7021 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//