LOCUS RC230447 6999 bp DNA circular SYN DEFINITION PCSK1 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 1 (PCSK1), transcript variant 2 ACCESSION NM_001177875 VERSION NM_001177875.2 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3156..3247 /label="Myc-DDK" misc_feature 3277..3862 /label="PolyA signal" misc_feature 3334..3353 /label="Restriction Enzyme" misc_feature 4138..4810 /label="ColE1" misc_feature 5240..6034 /label="Kan/Neo" misc_feature 6069..6371 /label="SV40 ori" misc_feature 6457..6934 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3138..3144 /label="Restriction Enzyme" misc_feature 1020..3138 /label="PCSK1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGGGAAGGGA TCTATTTCTT TCTTGTTCTT CAGCCAAATT GGTTCACTTG 1081 AAAATCACTA CTTATTCAAA CATAAAAACC ACCCCAGAAG GTCTCGAAGG AGTGCCTTTC 1141 ATATCACTAA GAGATTATCT GATGATGATC GTGTGATATG GGCTGAACAA CAGTATGAAA 1201 AAGAAAGAAG TAAACGTTCA GCTCTAAGGG ACTCAGCACT AAATCTCTTC AATGATCCCA 1261 TGTGGAATCA GCAATGGTAC TTGCAAGATA CCAGGATGAC GGCAGCCCTG CCCAAGCTGG 1321 ACCTTCATGT GATACCTGTT TGGCAAAAAG GCATTACGGG CAAAGGAGTT GTTATCACCG 1381 TACTGGATGA TGGTTTGGAG TGGAATCACA CGGACATTTA TGCCAACTAT GATCCAGAGG 1441 CTAGCTATGA TTTTAATGAT AATGACCATG ATCCATTTCC CCGATATGAT CCCACAAACG 1501 AGAACAAACA CGGGACCAGA TGTGCAGGAG AAATTGCCAT GCAAGCAAAT AATCACAAAT 1561 GCGGGGTTGG AGTTGCATAC AATTCCAAAG TTGGAGGCAT AAGAATGCTG GATGGCATTG 1621 TGACGGATGC TATTGAGGCC AGTTCAATTG GATTCAATCC TGGACACGTG GATATTTACA 1681 GTGCAAGCTG GGGCCCTAAT GATGATGGGA AAACTGTGGA GGGGCCTGGC CGGCTAGCCC 1741 AGAAGGCTTT TGAATATGGT GTCAAACAGG GGAGACAGGG GAAGGGGTCC ATCTTCGTCT 1801 GGGCTTCGGG AAACGGGGGG CGTCAGGGAG ATAATTGTGA CTGTGATGGC TACACAGACA 1861 GCATCTACAC CATCTCCATC AGCAGTGCCT CCCAGCAAGG CCTATCCCCC TGGTACGCTG 1921 AGAAGTGCTC CTCCACACTG GCCACCTCTT ACAGCAGCGG AGATTACACC GACCAGAGAA 1981 TCACGAGCGC TGACCTGCAC AATGACTGCA CGGAGACGCA CACAGGCACC TCGGCCTCTG 2041 CACCTCTGGC TGCTGGCATC TTCGCTCTGG CCCTGGAAGC AAACCCAAAT CTCACCTGGC 2101 GAGATATGCA GCACCTGGTT GTCTGGACCT CTGAGTATGA CCCGCTGGCC AATAACCCTG 2161 GATGGAAAAA GAATGGAGCA GGCTTGATGG TGAATAGTCG ATTTGGATTT GGCTTGCTAA 2221 ATGCCAAAGC TCTGGTGGAT TTAGCTGACC CCAGGACCTG GAGGAGCGTG CCTGAGAAGA 2281 AAGAGTGTGT TGTAAAGGAC AATGACTTTG AGCCCAGAGC CCTGAAAGCT AATGGAGAAG 2341 TTATCATTGA AATTCCAACA AGAGCTTGTG AAGGACAAGA AAATGCTATC AAGTCCCTGG 2401 AGCATGTACA ATTTGAAGCA ACAATTGAAT ATTCCCGAAG AGGAGACCTT CATGTCACAC 2461 TTACTTCTGC TGCTGGAACT AGCACTGTGC TCTTGGCTGA AAGAGAACGG GATACATCTC 2521 CTAATGGCTT TAAGAATTGG GACTTCATGT CTGTTCACAC ATGGGGAGAG AACCCTATAG 2581 GTACTTGGAC TTTGAGAATT ACAGACATGT CTGGAAGAAT TCAAAATGAA GGAAGAATTG 2641 TGAACTGGAA GCTGATTTTG CACGGGACCT CTTCTCAGCC AGAGCATATG AAGCAGCCTC 2701 GTGTGTACAC GTCCTACAAC ACTGTTCAGA ATGACAGAAG AGGGGTGGAG AAGATGGTGG 2761 ATCCAGGGGA GGAGCAGCCC ACACAAGAGA ACCCTAAGGA GAACACCCTG GTGTCCAAAA 2821 GCCCCAGCAG CAGCAGCGTA GGGGGCCGGA GGGATGAGTT GGAGGAGGGA GCCCCTTCCC 2881 AGGCCATGCT GCGACTCCTG CAAAGTGCTT TCAGTAAAAA CTCACCGCCA AAGCAATCAC 2941 CAAAGAAGTC CCCAAGTGCA AAGCTCAACA TCCCTTATGA AAACTTCTAC GAAGCCCTGG 3001 AAAAGCTGAA CAAACCTTCC CAGCTTAAAG ACTCTGAAGA CAGTCTGTAT AATGACTATG 3061 TTGATGTTTT TTATAACACT AAACCTTACA AGCACAGAGA CGACCGGCTG CTTCAAGCTC 3121 TGGTGGACAT TCTGAATGAG GAAAATACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 3181 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 3241 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 3301 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 3361 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 3421 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 3481 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 3541 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 3601 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 3661 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 3721 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 3781 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 3841 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 3901 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 3961 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 4021 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 4081 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 4141 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 4201 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 4261 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 4321 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 4381 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 4441 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 4501 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 4561 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 4621 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 4681 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 4741 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 4801 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 4861 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 4921 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 4981 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 5041 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 5101 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 5161 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 5221 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 5281 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 5341 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 5401 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 5461 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 5521 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 5581 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 5641 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 5701 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 5761 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 5821 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 5881 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 5941 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 6001 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 6061 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 6121 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 6181 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 6241 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 6301 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 6361 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 6421 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 6481 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 6541 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 6601 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 6661 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 6721 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 6781 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 6841 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 6901 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 6961 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT //