LOCUS       RC230377                6786 bp    DNA     circular  SYN 
DEFINITION  SYK (Myc-DDK-tagged)-Human spleen tyrosine kinase (SYK), transcript variant 3
ACCESSION   NM_001174167
VERSION     NM_001174167.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2943..3034 
                     /label="Myc-DDK"  
     misc_feature    3064..3649 
                     /label="PolyA signal"  
     misc_feature    3121..3140 
                     /label="Restriction Enzyme"  
     misc_feature    3925..4597 
                     /label="ColE1"  
     misc_feature    5027..5821 
                     /label="Kan/Neo"  
     misc_feature    5856..6158 
                     /label="SV40 ori"  
     misc_feature    6244..6721 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2925..2931 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2925 
                     /label="SYK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGCAGC GGCATGGCTG ACAGCGCCAA CCACCTGCCC TTCTTTTTCG 
     1081 GCAACATCAC CCGGGAGGAG GCAGAAGATT ACCTGGTCCA GGGGGGCATG AGTGATGGGC 
     1141 TTTATTTGCT GCGCCAGAGC CGCAACTACC TGGGTGGCTT CGCCCTGTCC GTGGCCCACG 
     1201 GGAGGAAGGC ACACCACTAC ACCATCGAGC GGGAGCTGAA TGGCACCTAC GCCATCGCCG 
     1261 GTGGCAGGAC CCATGCCAGC CCCGCCGACC TCTGCCACTA CCACTCCCAG GAGTCTGATG 
     1321 GCCTGGTCTG CCTCCTCAAG AAGCCCTTCA ACCGGCCCCA AGGGGTGCAG CCCAAGACTG 
     1381 GGCCCTTTGA GGATTTGAAG GAAAACCTCA TCAGGGAATA TGTGAAGCAG ACATGGAACC 
     1441 TGCAGGGTCA GGCTCTGGAG CAGGCCATCA TCAGTCAGAA GCCTCAGCTG GAGAAGCTGA 
     1501 TCGCTACCAC AGCCCATGAA AAAATGCCTT GGTTCCATGG AAAAATCTCT CGGGAAGAAT 
     1561 CTGAGCAAAT TGTCCTGATA GGATCAAAGA CAAATGGAAA GTTCCTGATC CGAGCCAGAG 
     1621 ACAACAACGG CTCCTACGCC CTGTGCCTGC TGCACGAAGG GAAGGTGCTG CACTATCGCA 
     1681 TCGACAAAGA CAAGACAGGG AAGCTCTCCA TCCCCGAGGG AAAGAAGTTC GACACGCTCT 
     1741 GGCAGCTAGT CGAGCATTAT TCTTATAAAG CAGATGGTTT GTTAAGAGTT CTTACTGTCC 
     1801 CATGTCAAAA AATCGGCACA CAGGGAAATG TTAATTTTGG AGGCCGTCCA CAACTTCCAG 
     1861 GTTCCCATCC TGCGACTTGG TCAGCGGGTG GAATAATCTC AAGAATCAAA TCATACTCCT 
     1921 TCCCAAAGCC TGGCCACAGA AAGTCCTCCC CTGCCCAAGG GAACCGGCAA GAGAGTACTG 
     1981 TGTCATTCAA TCCGTATGAG CCAGAACTTG CACCCTGGGC TGCAGACAAA GGCCCCCAGA 
     2041 GAGAAGCCCT ACCCATGGAC ACAGAGGTGT ACGAGAGCCC CTACGCGGAC CCTGAGGAGA 
     2101 TCAGGCCCAA GGAGGTTTAC CTGGACCGAA AGCTGCTGAC GCTGGAAGAC AAAGAACTGG 
     2161 GCTCTGGTAA TTTTGGAACT GTGAAAAAGG GCTACTACCA AATGAAAAAA GTTGTGAAAA 
     2221 CCGTGGCTGT GAAAATACTG AAAAACGAGG CCAATGACCC CGCTCTTAAA GATGAGTTAT 
     2281 TAGCAGAAGC AAATGTCATG CAGCAGCTGG ACAACCCGTA CATCGTGCGC ATGATCGGGA 
     2341 TATGCGAGGC CGAGTCCTGG ATGCTAGTTA TGGAGATGGC AGAACTTGGT CCCCTCAATA 
     2401 AGTATTTGCA GCAGAACAGA CATGTCAAGG ATAAGAACAT CATAGAACTG GTTCATCAGG 
     2461 TTTCCATGGG CATGAAGTAC TTGGAGGAGA GCAATTTTGT GCACAGAGAT CTGGCTGCAA 
     2521 GAAATGTGTT GCTAGTTACC CAACATTATG CCAAGATCAG TGATTTCGGA CTCTCCAAAG 
     2581 CACTGCGTGC TGATGAAAAC TACTACAAGG CCCAGACCCA TGGAAAGTGG CCTGTCAAGT 
     2641 GGTACGCTCC GGAATGCATC AACTACTACA AGTTCTCCAG CAAAAGCGAT GTCTGGAGCT 
     2701 TTGGAGTGTT GATGTGGGAA GCATTCTCCT ATGGGCAGAA GCCATATCGA GGGATGAAAG 
     2761 GAAGTGAAGT CACCGCTATG TTAGAGAAAG GAGAGCGGAT GGGGTGCCCT GCAGGGTGTC 
     2821 CAAGAGAGAT GTACGATCTC ATGAATCTGT GCTGGACATA CGATGTGGAA AACAGGCCCG 
     2881 GATTCGCAGC AGTGGAACTG CGGCTGCGCA ATTACTACTA TGACGTGGTG AACACGCGTA 
     2941 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3001 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3061 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3121 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3181 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3241 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3301 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3361 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3421 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3481 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3541 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3601 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     3661 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     3721 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     3781 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     3841 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     3901 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     3961 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4021 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4081 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4141 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4201 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4261 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4321 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4381 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4441 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4501 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4561 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4621 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     4681 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     4741 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     4801 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     4861 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     4921 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     4981 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5041 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5101 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5161 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5221 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5281 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5341 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5401 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5461 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5521 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5581 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     5641 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     5701 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     5761 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     5821 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     5881 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     5941 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6001 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6061 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6121 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6181 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6241 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6301 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6361 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6421 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6481 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6541 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6601 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     6661 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     6721 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     6781 AATTTT
//