LOCUS       RC230286                6579 bp    DNA     circular  SYN 
DEFINITION  NPNT (Myc-DDK-tagged)-Human nephronectin (NPNT), transcript variant 5
ACCESSION   NM_001184693
VERSION     NM_001184693.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2736..2827 
                     /label="Myc-DDK"  
     misc_feature    2857..3442 
                     /label="PolyA signal"  
     misc_feature    2914..2933 
                     /label="Restriction Enzyme"  
     misc_feature    3718..4390 
                     /label="ColE1"  
     misc_feature    4820..5614 
                     /label="Kan/Neo"  
     misc_feature    5649..5951 
                     /label="SV40 ori"  
     misc_feature    6037..6514 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2718..2724 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2718 
                     /label="NPNT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTTTCTC CTGGCGCTGG TGCTGGTATC CTCGCTCTAC CTGCAGGCGG 
     1081 CCGCCGAGTT CGACGGGAGG TGGCCCAGGC AAATAGTGTC ATCGATTGGC CTATGTCGTT 
     1141 ATGGTGGGAG GATTGACTGC TGCTGGGGCT GGGCTCGCCA GTCTTGGGGA CAGTGTCAGC 
     1201 CTGTGTGCCA ACCACGATGC AAACATGGTG AATGTATCGG GCCAAACAAG TGCAAGTGTC 
     1261 ATCCTGGTTA TGCTGGAAAA ACCTGTAATC AAGACGAGCA CATCCCAGCT CCTCTTGACC 
     1321 AAGGCAGTGA ACAGCCTCTT TTCCAACCCC TGGATCACCA AGCCACAAGT TTGCCTTCAA 
     1381 GGGATCTAAA TGAGTGTGGC CTGAAGCCCC GGCCCTGTAA GCACAGGTGC ATGAACACTT 
     1441 ACGGCAGCTA CAAGTGCTAC TGTCTCAACG GATATATGCT CATGCCGGAT GGTTCCTGCT 
     1501 CAAGTGCCCT GACCTGCTCC ATGGCAAACT GTCAGTATGG CTGTGATGTT GTTAAAGGAC 
     1561 AAATACGGTG CCAGTGCCCA TCCCCTGGCC TGCAGCTGGC TCCTGATGGG AGGACCTGTG 
     1621 TAGATGTTGA TGAATGTGCT ACAGGAAGAG CCTCCTGCCC TAGATTTAGG CAATGTGTCA 
     1681 ACACTTTTGG GAGCTACATC TGCAAGTGTC ATAAAGGCTT CGATCTCATG TATATTGGAG 
     1741 GCAAATATCA ATGTCATGAC ATAGACGAAT GCTCACTTGG TCAGTATCAG TGCAGCAGCT 
     1801 TTGCTCGATG TTATAACATA CGTGGGTCCT ACAAGTGCAA ATGTAAAGAA GGATACCAGG 
     1861 GTGATGGACT GACTTGTGTG TATATCCCAA AAGTTATGAT TGAACCTTCA GGTCCAATTC 
     1921 ATGTACCAAA GGGAAATGGT ACCATTTTAA AGGGTGACAC AGGAAATAAT AATTGGATTC 
     1981 CTGATGTTGG AAGTACTTGG TGGCCTCCGA AGACACCATA TATTCCTCCT ATCATTACCA 
     2041 ACAGGCCTAC TTCTAAGCCA ACAACAAGAC CTACACCAAA GCCAACACCA ATTCCTACTC 
     2101 CACCACCACC ACCACCCCTG CCAACAGAGC TCAGAACACC TCTACCACCT ACAACCCCAG 
     2161 AAAGGCCAAC CACCGGACTG ACAACTATAG CACCAGCTGC CAGTACACCT CCAGGAGGGA 
     2221 TTACAGTTGA CAACAGGGTA CAGACAGACC CTCAGAAACC CAGAGGAGAT GTGTTCAGTG 
     2281 TTCTGGTACA CAGTTGTAAT TTTGACCATG GACTTTGTGG ATGGATCAGG GAGAAAGACA 
     2341 ATGACTTGCA CTGGGAACCA ATCAGGGACC CAGCAGGTGG ACAATATCTG ACAGTGTCGG 
     2401 CAGCCAAAGC CCCAGGGGGA AAAGCTGCAC GCTTGGTGCT ACCTCTCGGC CGCCTCATGC 
     2461 ATTCAGGGGA CCTGTGCCTG TCATTCAGGC ACAAGGTGAC GGGGCTGCAC TCTGGCACAC 
     2521 TCCAGGTGTT TGTGAGAAAA CACGGTGCCC ACGGAGCAGC CCTGTGGGGA AGAAATGGTG 
     2581 GCCATGGCTG GAGGCAAACA CAGATCACCT TGCGAGGGGC TGACATCAAG AGCGTCGTCT 
     2641 TCAAAGGTGA AAAAAGGCGT GGTCACACTG GGGAGATTGG ATTAGATGAT GTGAGCTTGA 
     2701 AAAAAGGCCA CTGCTCTGAA GAACGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     2761 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     2821 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     2881 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     2941 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3001 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3061 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3121 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3181 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3241 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3301 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3361 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     3421 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     3481 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     3541 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     3601 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     3661 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     3721 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     3781 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     3841 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     3901 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     3961 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4021 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4081 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4141 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4201 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4261 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4321 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4381 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     4441 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     4501 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     4561 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     4621 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     4681 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     4741 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     4801 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     4861 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     4921 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     4981 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5041 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5101 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5161 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5221 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5281 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5341 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     5401 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     5461 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     5521 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     5581 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     5641 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     5701 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     5761 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     5821 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     5881 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     5941 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6001 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6061 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6121 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6181 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6241 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6301 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6361 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     6421 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     6481 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     6541 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//