LOCUS       RC229432                8910 bp    DNA     circular  SYN 
DEFINITION  SYNGAP1 (Myc-DDK-tagged)-Human synaptic Ras GTPase activating protein 1 (SYNGAP1)
ACCESSION   NM_006772
VERSION     NM_006772.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5067..5158 
                     /label="Myc-DDK"  
     misc_feature    5188..5773 
                     /label="PolyA signal"  
     misc_feature    5245..5264 
                     /label="Restriction Enzyme"  
     misc_feature    6049..6721 
                     /label="ColE1"  
     misc_feature    7151..7945 
                     /label="Kan/Neo"  
     misc_feature    7980..8282 
                     /label="SV40 ori"  
     misc_feature    8368..8845 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5049..5055 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5049 
                     /label="SYNGAP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCAGGTCT CGAGCCTCCA TCCATCGGGG GAGCATCCCC GCGATGTCCT 
     1081 ATGCCCCCTT CAGAGATGTA CGGGGACCCT CTATGCACCG AACCCAATAC GTTCATTCCC 
     1141 CGTATGATCG TCCTGGTTGG AACCCTCGGT TCTGCATCAT CTCGGGGAAC CAGCTGCTCA 
     1201 TGCTGGATGA GGATGAGATA CACCCCCTAC TGATCCGGGA CCGGAGGAGC GAGTCCAGTC 
     1261 GCAACAAACT GCTGAGACGC ACAGTCTCCG TGCCGGTGGA GGGGCGGCCC CACGGCGAGC 
     1321 ATGAATACCA CTTGGGTCGC TCGAGGAGGA AGAGTGTCCC AGGGGGGAAG CAGTACAGCA 
     1381 TGGAGGGTGC CCCTGCTGCG CCCTTCCGGC CCTCGCAAGG CTTCCTGAGC CGACGGCTAA 
     1441 AAAGCTCCAT CAAACGAACG AAGTCACAAC CCAAACTTGA CCGGACCAGC AGCTTTCGCC 
     1501 AGATCCTGCC TCGCTTCCGA AGTGCTGACC ATGACCGGGC CCGGCTGATG CAAAGCTTTA 
     1561 AGGAGTCACA CTCTCATGAG TCCTTGCTGA GTCCTAGCAG TGCAGCTGAG GCATTGGAGC 
     1621 TCAACTTGGA TGAAGATTCC ATTATCAAGC CAGTGCACAG CTCCATCCTG GGCCAGGAGT 
     1681 TCTGTTTTGA GGTAACAACT TCATCAGGAA CAAAATGCTT TGCCTGTCGG TCTGCGGCCG 
     1741 AAAGAGACAA ATGGATTGAG AATCTGCAGC GGGCAGTAAA GCCCAACAAG GACAACAGCC 
     1801 GCCGGGTAGA CAATGTGCTA AAGCTGTGGA TCATAGAGGC CCGGGAGCTG CCCCCCAAGA 
     1861 AGCGGTACTA CTGTGAGCTC TGCCTGGATG ACATGCTGTA TGCACGCACC ACCTCCAAGC 
     1921 CCCGCTCTGC CTCTGGGGAC ACCGTCTTCT GGGGCGAGCA CTTCGAGTTT AACAACCTGC 
     1981 CGGCTGTCCG TGCCCTGCGG CTGCATCTGT ACCGTGACTC AGACAAAAAG CGCAAGAAGG 
     2041 ACAAGGCAGG CTATGTCGGC CTGGTGACTG TGCCAGTGGC CACCCTGGCT GGGCGCCACT 
     2101 TCACAGAGCA GTGGTACCCT GTAACCCTGC CAACAGGCAG TGGGGGATCT GGGGGCATGG 
     2161 GTTCGGGAGG GGGAGGGGGC TCGGGGGGTG GCTCAGGGGG CAAGGGCAAA GGAGGTTGCC 
     2221 CGGCTGTGCG GCTGAAAGCA CGTTACCAGA CAATGAGCAT CTTGCCCATG GAGCTATATA 
     2281 AAGAGTTTGC AGAGTATGTC ACCAACCATT ATCGGATGCT GTGTGCAGTC TTGGAGCCCG 
     2341 CCCTGAATGT CAAAGGCAAG GAGGAGGTTG CCAGTGCACT AGTTCACATC CTGCAGAGTA 
     2401 CAGGCAAGGC CAAGGACTTC CTTTCAGACA TGGCCATGTC TGAGGTAGAC CGGTTCATGG 
     2461 AACGGGAGCA CCTCATATTC CGCGAGAACA CGCTTGCCAC TAAAGCCATA GAAGAGTATA 
     2521 TGAGACTGAT TGGTCAGAAA TACCTCAAGG ATGCCATTGG AGAATTCATC CGTGCTCTGT 
     2581 ATGAATCTGA GGAAAACTGC GAGGTAGACC CTATCAAGTG CACAGCATCC AGTTTGGCAG 
     2641 AGCACCAGGC CAACCTGCGA ATGTGCTGTG AGTTGGCCCT GTGCAAGGTG GTCAACTCCC 
     2701 ACTGCGTGTT CCCGAGGGAG CTGAAGGAGG TGTTTGCTTC GTGGCGGCTG CGCTGCGCAG 
     2761 AGCGAGGCCG GGAGGACATC GCAGACAGGC TTATCAGCGC CTCACTCTTC CTGCGCTTCC 
     2821 TCTGCCCAGC GATTATGTCG CCCAGTCTCT TTGGGCTTAT GCAGGAGTAC CCAGATGAGC 
     2881 AGACCTCACG AACCCTCACC CTCATTGCCA AGGTCATCCA GAACCTGGCC AACTTTTCCA 
     2941 AGTTTACCTC AAAGGAGGAC TTTCTGGGCT TCATGAATGA GTTTCTGGAG CTGGAATGGG 
     3001 GTTCCATGCA GCAGTTTTTG TATGAGATCT CCAATCTGGA CACGCTAACC AACAGCAGTA 
     3061 GCTTTGAGGG TTACATCGAC TTGGGCCGAG AGCTCTCCAC ACTGCATGCC CTACTCTGGG 
     3121 AGGTGCTGCC CCAGCTCAGC AAGGAAGCCC TCCTGAAGCT GGGTCCACTG CCCCGGCTCC 
     3181 TCAACGACAT CAGCACAGCT CTGAGGAACC CCAACATCCA AAGGCAGCCA AGCCGCCAGA 
     3241 GTGAGCGGCC CCGGCCTCAG CCTGTGGTAC TGCGGGGGCC ATCGGCTGAG ATGCAGGGCT 
     3301 ACATGATGCG GGACCTCAAC AGCTCCATCG ACCTTCAGTC CTTCATGGCT CGAGGCCTCA 
     3361 ACAGCTCTAT GGACATGGCT CGCCTCCCCT CCCCAACCAA GGAAAAGCCA CCCCCACCAC 
     3421 CGCCTGGTGG TGGTAAAGAC CTGTTCTATG TAAGCCGTCC ACCCCTGGCC CGTTCCTCAC 
     3481 CAGCATACTG CACGAGCAGC TCGGACATCA CAGAGCCAGA GCAGAAGATG CTGAGTGTCA 
     3541 ACAAGAGTGT GTCCATGCTG GACTTACAGG GTGATGGGCC TGGTGGCCGC CTCAACAGCA 
     3601 GCAGTGTTTC GAACCTGGCG GCCGTAGGGG ACCTGCTGCA CTCAAGCCAG GCCTCGCTGA 
     3661 CAGCAGCCTT GGGGCTACGG CCTGCGCCTG CCGGACGCCT CTCCCAGGGG AGTGGCTCAT 
     3721 CCATCACGGC GGCTGGCATG CGCCTCAGCC AGATGGGTGT CACCACAGAC GGTGTCCCTG 
     3781 CCCAGCAACT GCGAATCCCC CTCTCCTTCC AGAACCCTCT CTTCCACATG GCTGCTGATG 
     3841 GGCCAGGTCC CCCAGGCGGC CATGGAGGGG GCGGTGGCCA TGGCCCACCT TCCTCCCATC 
     3901 ACCACCACCA CCACCATCAC CACCACCGAG GTGGAGAGCC CCCTGGGGAC ACCTTTGCCC 
     3961 CATTCCATGG CTATAGCAAG AGTGAGGACC TCTCTTCCGG GGTCCCCAAG CCCCCTGCTG 
     4021 CCTCCATCCT TCATAGCCAC AGCTACAGTG ATGAGTTTGG ACCCTCTGGC ACTGACTTCA 
     4081 CCCGTCGGCA GCTTTCACTC CAGGACAACC TGCAGCACAT GCTGTCCCCT CCCCAGATCA 
     4141 CCATTGGTCC CCAGAGGCCA GCCCCCTCAG GGCCTGGAGG TGGGAGCGGT GGGGGCAGCG 
     4201 GTGGGGGTGG CGGGGGCCAG CCGCCTCCAT TGCAGAGGGG CAAGTCTCAG CAGTTGACAG 
     4261 TCAGCGCAGC CCAGAAACCC CGGCCATCCA GCGGGAATCT ATTGCAGTCC CCAGAGCCAA 
     4321 GTTATGGCCC CGCCCGTCCA CGGCAACAGA GCCTCAGCAA GGAGGGCAGC ATTGGGGGCA 
     4381 GCGGGGGCAG CGGTGGCGGA GGGGGTGGGG GGCTGAAGCC CTCCATCACC AAGCAGCATT 
     4441 CTCAGACACC ATCCACATTG AACCCCACAA TGCCAGCCTC TGAGCGGACA GTGGCCTGGG 
     4501 TCTCCAACAT GCCTCACCTG TCGGCTGACA TCGAGAGTGC CCACATCGAG CGGGAAGAGT 
     4561 ACAAGCTCAA GGAGTACTCA AAATCGATGG ATGAGAGCCG GCTGGATAGG GTGAAGGAGT 
     4621 ACGAGGAGGA GATTCACTCA CTGAAAGAGC GGCTGCACAT GTCCAACCGG AAGCTGGAAG 
     4681 AGTATGAGCG GAGGCTGCTG TCCCAGGAAG AACAAACCAG CAAAATCCTG ATGCAGTATC 
     4741 AGGCCCGACT GGAGCAGAGT GAGAAGAGGC TAAGGCAGCA GCAGGCAGAG AAGGATTCCC 
     4801 AGATCAAGAG CATCATTGGC AGGCTGATGC TGGTGGAGGA GGAGCTGCGC CGGGACCACC 
     4861 CCGCCATGGC TGAGCCGCTG CCAGAACCCA AGAAGAGGCT GCTCGACGCT CAGGAGAGGC 
     4921 AGCTTCCCCC CTTGGGTCCA ACAAACCCGC GTGTGACGCT GGCCCCACCG TGGAATGGCC 
     4981 TGGCCCCCCC AGCCCCACCA CCCCCACCCC GGCTGCAGAT TACGGAGAAC GGCGAGTTCC 
     5041 GAAACACCGC AGACCACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5101 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5161 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5221 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5281 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5341 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5401 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5461 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5521 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5581 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5641 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5701 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5761 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5821 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5881 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5941 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6001 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6061 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6121 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6181 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6241 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6301 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6361 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6421 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6481 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6541 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6601 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6661 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6721 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6781 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6841 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6901 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6961 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7021 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7081 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7141 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7201 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7261 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7321 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7381 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7441 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7501 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7561 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7621 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7681 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7741 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7801 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7861 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7921 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7981 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8041 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8101 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8161 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8221 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8281 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8341 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8401 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8461 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8521 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8581 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8641 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8701 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8761 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8821 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8881 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//