LOCUS       RC229421                8628 bp    DNA     circular  SYN 
DEFINITION  ITSN2 (Myc-DDK-tagged)-Human intersectin 2 (ITSN2), transcript variant 2
ACCESSION   NM_147152
VERSION     NM_147152.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4785..4876 
                     /label="Myc-DDK"  
     misc_feature    4906..5491 
                     /label="PolyA signal"  
     misc_feature    4963..4982 
                     /label="Restriction Enzyme"  
     misc_feature    5767..6439 
                     /label="ColE1"  
     misc_feature    6869..7663 
                     /label="Kan/Neo"  
     misc_feature    7698..8000 
                     /label="SV40 ori"  
     misc_feature    8086..8563 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4773 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="ITSN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCTCAG TTTCCCACAG CTATGAATGG AGGGCCAAAC ATGTGGGCTA 
     1081 TTACCTCTGA AGAACGTACT AAGCATGACA GGCAGTTTGA TAACCTCAAA CCTTCAGGAG 
     1141 GTTACATAAC AGGTGATCAA GCACGTAATT TTTTCCTACA ATCAGGTCTG CCGGCCCCTG 
     1201 TTTTAGCTGA AATATGGGCT TTATCAGACC TAAACAAGGA TGGGAAGATG GATCAGCAAG 
     1261 AGTTCTCCAT AGCTATGAAA CTCATCAAAC TGAAGCTTCA AGGCCAACAG TTGCCTGTGG 
     1321 TTCTCCCTCC TATTATGAAG CAACCCCCTA TGTTTTCTCC ATTAATTTCT GCTCGTTTTG 
     1381 GAATGGGAAG CATGCCCAAT CTGTCCATTC CTCAGCCATT GCCTCCAGCT GCACCTATAA 
     1441 CATCATTGTC TTCTGCGACT TCAGGGACCA ACCTTCCTCC CTTAATGATG CCCACTCCCC 
     1501 TAGTGCCTTC TGTTAGCACA TCATCATTAC CAAATGGAAC CGCCAGTCTC ATTCAGCCTT 
     1561 TACCCATTCC TTATTCTTCT TCAACATTGC CTCATGGGTC ATCTTATAGT CTGATGATGG 
     1621 GAGGATTTGG AGGTGCTAGT ATACAGAAAG CGCAGTCTCT GATTGATTTA GGATCTAGTA 
     1681 GCTCAACTTC CTCGACTGCT TCACTCTCAG GGAACTCACC CAAGACTGGG ACCTCAGAGT 
     1741 GGGCAGTTCC TCAGCCTACA AGATTAAAAT ATCGGCAAAA ATTTAATACT CTTGACAAAA 
     1801 GTATGAGTGG ATATCTCTCA GGTTTTCAAG CTAGAAATGC CCTTCTTCAG TCAAATCTTT 
     1861 CTCAAACTCA GCTGGCTACT ATTTGGACTC TGGCTGACGT TGATGGTGAT GGACAGCTAA 
     1921 AAGCAGAAGA GTTTATTCTT GCAATGCACC TTACTGACAT GGCCAAAGCT GGACAGCCAT 
     1981 TACCACTGAC TTTACCTCCT GAGCTTGTTC CTCCATCTTT CAGAGGAGGA AAGCAAATTG 
     2041 ATTCCATTAA TGGAACTCTG CCTTCATATC AGAAAATGCA AGAAGAGGAG CCTCAGAAGA 
     2101 AATTACCAGT TACTTTTGAG GACAAACGGA AAGCCAACTA TGAGCGAGGG AACATGGAGC 
     2161 TGGAAAAGCG ACGCCAAGCC TTGATGGAGC AGCAACAAAG GGAGGCAGAA CGTAAAGCCC 
     2221 AGAAAGAAAA GGAAGAGTGG GAACGAAAAC AGAGAGAATT ACAAGAACAA GAATGGAAGA 
     2281 AACAACTTGA ATTAGAAAAA CGCTTAGAGA AGCAACGGGA ATTGGAGAGA CAACGAGAGG 
     2341 AAGAAAGGAG AAAAGACATA GAAAGACGAG AGGCAGCAAA ACAGGAACTT GAACGACAAC 
     2401 GTCGCTTAGA ATGGGAGAGA ATTCGGCGAC AGGAGCTTCT CAATCAAAAG AATAGAGAAC 
     2461 AAGAAGAAAT TGTCAGGTTA AACTCTAAAA AGAAGAATCT TCATCTTGAG TTGGAAGCAC 
     2521 TGAATGGCAA ACATCAGCAG ATCTCAGGCA GACTTCAGGA TGTCCGACTC AAAAAGCAAA 
     2581 CTCAAAAGAC TGAGCTGGAA GTTCTGGATA AGCAGTGTGA CTTGGAAATT ATGGAAATCA 
     2641 AGCAACTTCA ACAGGAACTT CAGGAATATC AGAATAAGCT TATCTATCTG GTACCTGAGA 
     2701 AGCAATTATT AAATGAAAGA ATTAAAAACA TGCAGTTCAG TAACACACCT GATTCAGGGG 
     2761 TCAGTTTACT TCATAAAAAA TCATTAGAAA AGGAAGAATT ATGCCAAAGA CTTAAAGAAC 
     2821 AGTTAGATGC TCTTGAAAAA GAAACTGCAT CTAAGCTGTC AGAAATGGAT TCTTTTAACA 
     2881 ATCAACTAAA GTGTGGGAAT ATGGATGACT CTGTTCTTCA GTGCCTTTTG TCTCTGCTAA 
     2941 GCTGTCTCAA CAACCTCTTC CTCTTACTTA AGGAACTGAG AGAAACCTAC AACACACAGC 
     3001 AGTTAGCCCT TGAACAGCTT TATAAGATCA AACGTGACAA GTTGAAGGAA ATTGAAAGGA 
     3061 AAAGATTAGA ACTAATGCAG AAAAAGAAAC TAGAAGATGA GGCTGCAAGG AAAGCAAAGC 
     3121 AAGGAAAAGA AAACTTATGG AAAGAAAATC TTAGAAAGGA GGAAGAAGAA AAACAAAAGC 
     3181 GACTCCAGGA AGAAAAAACA CAAGAAAAAA TTCAAGAAGA GGAACGGAAA GCTGAGGAGA 
     3241 AACAACGTAA GGATAAGGAT ACTTTGAAAG CTGAGGAGAA AAAACGTGAG ACAGCTAGTG 
     3301 TTTTGGTGAA TTATAGAGCA TTATACCCCT TTGAAGCAAG GAACCATGAT GAGATGAGTT 
     3361 TTAATTCTGG AGATATAATT CAGGTTGATG AAAAAACCGT AGGAGAACCT GGTTGGCTTT 
     3421 ATGGTAGTTT TCAAGGAAAT TTTGGCTGGT TTCCATGCAA TTATGTAGAA AAAATGCCAT 
     3481 CAAGTGAAAA TGAAAAAGCT GTATCTCCAA AGAAGGCCTT ACTTCCTCCT ACAGTTTCTT 
     3541 TATCTGCTAC CTCAACTTCC TCTGAACCAC TTTCTTCAAA TCAACCAGCA TCAGTGACTG 
     3601 ATTATCAAAA TGTATCTTTT TCAAACCTAA CTGTAAATAC ATCATGGCAG AAAAAATCAG 
     3661 CCTTCACTCG AACTGTGTCC CCTGGATCTG TATCACCTAT TCATGGACAG GGACAAGTGG 
     3721 TAGAAAACTT AAAAGCACAG GCCCTTTGTT CCTGGACTGC AAAGAAAGAT AACCACTTGA 
     3781 ACTTCTCAAA ACATGACATT ATTACTGTCT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG 
     3841 AGGTGCATGG AGGAAGAGGA TGGTTTCCCA AATCTTATGT CAAGATCATT CCTGGGAGTG 
     3901 AAGTAAAACG GGAAGAACCA GAAGCTTTGT ATGCAGCTGT AAATAAGAAA CCTACCTCGG 
     3961 CAGCCTATTC AGTTGGAGAA GAATATATTG CACTTTATCC ATATTCAAGT GTGGAACCTG 
     4021 GAGATTTGAC TTTCACAGAA GGTGAAGAAA TATTGGTGAC CCAGAAAGAT GGAGAGTGGT 
     4081 GGACAGGAAG TATTGGAGAT AGAAGTGGAA TTTTTCCATC AAACTATGTC AAACCAAAGG 
     4141 ATCAAGAGAG TTTTGGGAGT GCTAGCAAGT CTGGAGCATC AAATAAAAAA CCTGAGATTG 
     4201 CTCAGGTAAC TTCAGCATAT GTTGCTTCTG GTTCTGAACA ACTTAGCCTT GCACCAGGAC 
     4261 AGTTAATATT AATTCTAAAG AAAAATACAA GTGGGTGGTG GCAAGGAGAG TTACAGGCCA 
     4321 GAGGAAAAAA GCGACAGAAA GGATGGTTTC CTGCCAGTCA TGTTAAACTT TTGGGTCCAA 
     4381 GTAGTGAAAG AGCCACACCT GCCTTTCATC CTGTATGTCA GGTGATTGCT ATGTATGACT 
     4441 ATGCAGCAAA TAATGAAGAT GAGCTCAGTT TCTCCAAGGG ACAACTCATT AATGTTATGA 
     4501 ACAAAGATGA TCCTGATTGG TGGCAAGGAG AGATCAACGG GGTGACTGGT CTCTTTCCTT 
     4561 CAAACTACGT TAAGATGACG ACAGACTCAG ATCCAAGTCA ACAGTGGTGT GCTGATCTGC 
     4621 AAACCCTGGA CACAATGCAG CCAATTGAGA GGAAAAGACA GGGCTATATT CATGAGCTGA 
     4681 TTCAGACCGA AGAGCGGTAC ATGGCTGACC TTCAGCTCGT CGTCGAGGTG AGGAGGCTGC 
     4741 TGCTGGCTAG CTCTCGGGGT ATCTGCTGTC TCTCAACGCG TACGCGGCCG CTCGAGCAGA 
     4801 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4861 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4921 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4981 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5041 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5101 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5161 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5221 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5281 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5341 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5401 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5461 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5521 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5581 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5641 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5761 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5821 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5881 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5941 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6061 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6121 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6181 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6241 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6361 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6421 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6481 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6541 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6601 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6661 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6721 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6781 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6841 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6901 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6961 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7021 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7081 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7141 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7201 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7261 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7321 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7381 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7441 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7501 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7561 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7621 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7681 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7741 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7801 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7861 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8041 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8101 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8161 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8401 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8521 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//