LOCUS       RC229420                8598 bp    DNA     circular  SYN 
DEFINITION  MECOM (Myc-DDK-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 4
ACCESSION   NM_004991
VERSION     NM_004991.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4755..4846 
                     /label="Myc-DDK"  
     misc_feature    4876..5461 
                     /label="PolyA signal"  
     misc_feature    4933..4952 
                     /label="Restriction Enzyme"  
     misc_feature    5737..6409 
                     /label="ColE1"  
     misc_feature    6839..7633 
                     /label="Kan/Neo"  
     misc_feature    7668..7970 
                     /label="SV40 ori"  
     misc_feature    8056..8533 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4737..4743 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4737 
                     /label="MECOM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGATCCAAA GGCAGGGCAA GGAAACTGGC CACAAATAAT GAGTGTGTAT 
     1081 ATGGCAACTA CCCTGAAATA CCTTTGGAAG AAATGCCAGA TGCAGATGGA GTAGCCAGCA 
     1141 CTCCCTCCCT CAATATTCAA GAGCCATGCT CTCCTGCCAC ATCCAGTGAA GCATTCACTC 
     1201 CAAAGGAGGG TTCTCCTTAC AAAGCCCCCA TCTACATCCC TGATGATATC CCCATTCCTG 
     1261 CTGAGTTTGA ACTTCGAGAG TCAAATATGC CTGGGGCAGG ACTAGGAATA TGGACCAAAA 
     1321 GGAAGATCGA AGTAGGTGAA AAGTTTGGGC CTTATGTGGG AGAGCAGAGG TCAAACCTGA 
     1381 AAGACCCCAG TTATGGATGG GAGATCTTAG ACGAATTTTA CAATGTGAAG TTCTGCATAG 
     1441 ATGCCAGTCA ACCAGATGTT GGAAGCTGGC TCAAGTACAT TAGATTCGCT GGCTGTTATG 
     1501 ATCAGCACAA CCTTGTTGCA TGCCAGATAA ATGATCAGAT ATTCTATAGA GTAGTTGCAG 
     1561 ACATTGCGCC GGGAGAGGAG CTTCTGCTGT TCATGAAGAG CGAAGACTAT CCCCATGAAA 
     1621 CTATGGCGCC GGATATCCAC GAAGAACGGC AATATCGCTG CGAAGACTGT GACCAGCTCT 
     1681 TTGAATCTAA GGCTGAACTA GCAGATCACC AAAAGTTTCC ATGCAGTACT CCTCACTCAG 
     1741 CATTTTCAAT GGTTGAAGAG GACTTTCAGC AAAAACTCGA AAGCGAGAAT GATCTCCAAG 
     1801 AGATACACAC GATCCAGGAG TGTAAGGAAT GTGACCAAGT TTTTCCTGAT TTGCAAAGCC 
     1861 TGGAGAAACA CATGCTGTCA CATACTGAAG AGAGGGAATA CAAGTGTGAT CAGTGTCCCA 
     1921 AGGCATTTAA CTGGAAGTCC AATTTAATTC GCCACCAGAT GTCACATGAC AGTGGAAAGC 
     1981 ACTATGAATG TGAAAACTGT GCCAAGGTTT TCACGGACCC TAGCAACCTT CAGCGGCACA 
     2041 TTCGCTCTCA GCATGTCGGT GCCCGGGCCC ATGCATGCCC GGAGTGTGGC AAAACGTTTG 
     2101 CCACTTCGTC GGGCCTCAAA CAACACAAGC ACATCCACAG CAGTGTGAAG CCCTTTATCT 
     2161 GTGAGGTCTG CCATAAATCC TATACTCAGT TTTCAAACCT TTGCCGTCAT AAGCGCATGC 
     2221 ATGCTGATTG CAGAACCCAA ATCAAGTGCA AAGACTGTGG ACAAATGTTC AGCACTACGT 
     2281 CTTCCTTAAA TAAACACAGG AGGTTTTGTG AGGGCAAGAA CCATTTTGCG GCAGGTGGAT 
     2341 TTTTTGGCCA AGGCATTTCA CTTCCTGGAA CCCCAGCTAT GGATAAAACG TCCATGGTTA 
     2401 ATATGAGTCA TGCCAACCCG GGCCTTGCTG ACTATTTTGG CGCCAATAGG CATCCTGCTG 
     2461 GTCTTACCTT TCCAACAGCT CCTGGATTTT CTTTTAGCTT CCCTGGTCTG TTTCCTTCCG 
     2521 GCTTGTACCA CAGGCCTCCT TTGATACCTG CTAGTTCTCC TGTTAAAGGA CTATCAAGTA 
     2581 CTGAACAGAC AAACAAAAGT CAAAGTCCCC TCATGACACA TCCTCAGATA CTGCCAGCTA 
     2641 CACAGGATAT TTTGAAGGCA CTATCTAAAC ACCCATCTGT AGGGGACAAT AAGCCAGTGG 
     2701 AGCTCCAGCC CGAGAGGTCC TCTGAAGAGA GGCCCTTTGA GAAAATCAGT GACCAGTCAG 
     2761 AGAGTAGTGA CCTTGATGAT GTCAGTACAC CAAGTGGCAG TGACCTGGAA ACAACCTCGG 
     2821 GCTCTGATCT GGAAAGTGAC ATTGAAAGTG ATAAAGAGAA ATTTAAAGAA AATGGTAAAA 
     2881 TGTTCAAAGA CAAAGTAAGC CCTCTTCAGA ATCTGGCTTC AATAAATAAT AAGAAAGAAT 
     2941 ACAGCAATCA TTCCATTTTC TCACCATCTT TAGAGGAGCA GACTGCGGTG TCAGGAGCTG 
     3001 TGAATGATTC TATAAAGGCT ATTGCTTCTA TTGCTGAAAA ATACTTTGGT TCAACAGGAC 
     3061 TGGTGGGGCT GCAAGACAAA AAAGTTGGAG CTTTACCTTA CCCTTCCATG TTTCCCCTCC 
     3121 CATTTTTTCC AGCATTCTCT CAATCAATGT ACCCATTTCC TGATAGAGAC TTGAGATCGT 
     3181 TACCTTTGAA AATGGAACCC CAATCACCAG GTGAAGTAAA GAAACTGCAG AAGGGCAGCT 
     3241 CTGAGTCCCC CTTTGATCTC ACCACTAAGC GAAAGGATGA GAAGCCCTTG ACTCCAGTCC 
     3301 CCTCCAAGCC TCCAGTGACA CCTGCCACAA GCCAAGACCA GCCCCTGGAT CTAAGTATGG 
     3361 GCAGTAGGAG TAGAGCCAGT GGGACAAAGC TGACTGAGCC TCGAAAAAAC CACGTGTTTG 
     3421 GGGGAAAAAA AGGAAGCAAC GTCGAATCAA GACCTGCTTC AGATGGTTCC TTGCAGCATG 
     3481 CAAGACCCAC TCCTTTCTTT ATGGACCCTA TTTACAGAGT AGAGAAAAGA AAACTAACTG 
     3541 ACCCACTTGA AGCTTTAAAA GAGAAATACT TGAGGCCTTC TCCAGGATTC TTGTTTCACC 
     3601 CACAATTCCA ACTGCCTGAT CAGAGAACTT GGATGTCAGC TATTGAAAAC ATGGCAGAAA 
     3661 AGCTAGAGAG CTTCAGTGCC CTGAAACCTG AGGCCAGTGA GCTCTTACAG TCAGTGCCCT 
     3721 CTATGTTCAA CTTCAGGGCG CCTCCCAATG CCCTGCCAGA GAACCTTCTG CGGAAGGGAA 
     3781 AGGAGCGCTA TACCTGCAGA TACTGTGGCA AGATTTTTCC AAGGTCTGCA AACCTAACAC 
     3841 GGCACTTGAG AACCCACACA GGAGAGCAGC CTTACAGATG CAAATACTGT GACAGATCAT 
     3901 TTAGCATATC TTCTAACTTG CAAAGGCATG TTCGCAACAT CCACAATAAA GAGAAGCCAT 
     3961 TTAAGTGTCA CTTATGTGAT AGGTGTTTTG GTCAACAAAC CAATTTAGAC AGACACCTAA 
     4021 AGAAACATGA GAATGGGAAC ATGTCCGGTA CAGCAACATC GTCGCCTCAT TCTGAACTGG 
     4081 AAAGTACAGG TGCGATTCTG GATGACAAAG AAGATGCTTA CTTCACAGAA ATTCGAAATT 
     4141 TCATTGGGAA CAGCAACCAT GGCAGCCAAT CTCCCAGGAA TGTGGAGGAG AGAATGAATG 
     4201 GCAGTCATTT TAAAGATGAA AAGGCTTTGG TGACCAGTCA AAATTCAGAC TTGCTGGATG 
     4261 ATGAAGAAGT TGAAGATGAG GTGTTGTTAG ATGAGGAGGA TGAAGACAAT GATATTACTG 
     4321 GAAAAACAGG AAAGGAACCA GTGACAAGTA ATTTACATGA AGGAAACCCT GAGGATGACT 
     4381 ATGAAGAAAC CAGTGCCCTG GAGATGAGTT GCAAGACATC CCCAGTGAGG TATAAAGAGG 
     4441 AAGAATATAA AAGTGGACTT TCTGCTCTAG ATCATATAAG GCACTTCACA GATAGCCTCA 
     4501 AAATGAGGAA AATGGAAGAT AATCAATATT CTGAAGCTGA GCTGTCTTCT TTTAGTACTT 
     4561 CCCATGTGCC AGAGGAACTT AAGCAGCCGT TACACAGAAA GTCCAAATCG CAGGCATATG 
     4621 CTATGATGCT GTCACTGTCT GACAAGGAGT CCCTCCATTC TACATCCCAC AGTTCTTCCA 
     4681 ACGTGTGGCA CAGTATGGCC AGGGCTGCGG CGGAATCCAG TGCTATCCAG TCCATAAGCC 
     4741 ACGTAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4801 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4861 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4921 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4981 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5041 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5101 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5161 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5221 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5281 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5341 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5401 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5461 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5521 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5581 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5641 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5701 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5761 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5821 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5881 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5941 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6001 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6061 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6121 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6181 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6241 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6301 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6361 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6421 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6481 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6541 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6601 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6661 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6721 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6781 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6841 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6901 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6961 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7021 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7081 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7141 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7201 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7261 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7321 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7381 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7441 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7501 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7561 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7621 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7681 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7741 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7801 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7861 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7921 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7981 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8041 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8101 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8161 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8221 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8281 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8341 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8401 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8461 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8521 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8581 AAATTTAACG CGAATTTT
//