LOCUS       RC229406                8013 bp    DNA     circular  SYN 
DEFINITION  MEGF11 (Myc-DDK-tagged)-Human multiple EGF-like-domains 11 (MEGF11)
ACCESSION   NM_032445
VERSION     NM_032445.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4170..4261 
                     /label="Myc-DDK"  
     misc_feature    4291..4876 
                     /label="PolyA signal"  
     misc_feature    4348..4367 
                     /label="Restriction Enzyme"  
     misc_feature    5152..5824 
                     /label="ColE1"  
     misc_feature    6254..7048 
                     /label="Kan/Neo"  
     misc_feature    7083..7385 
                     /label="SV40 ori"  
     misc_feature    7471..7948 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4152..4158 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4152 
                     /label="MEGF11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCTCTCC CTGACGGGGC TCATTGCCTT CTCCTTCCTG CAAGCCACCC 
     1081 TTGCCCTGAA CCCCGAGGAC CCCAACGTGT GCAGCCACTG GGAGAGCTAT GCTGTGACTG 
     1141 TCCAGGAATC GTATGCACAC CCCTTCGATC AGATCTATTA CACACGATGC ACAGACATCC 
     1201 TCAACTGGTT CAAGTGCACC AGGCACCGGA TCAGTTATAA GACGGCGTAT CGGAGAGGCC 
     1261 TCCGGACCAT GTACCGGCGG AGGTCCCAGT GCTGCCCTGG CTACTATGAG AGCGGAGACT 
     1321 TCTGCATACC CCTGTGTACG GAGGAGTGTG TGCACGGCCG CTGCGTTTCC CCGGACACCT 
     1381 GCCACTGCGA GCCTGGCTGG GGAGGGCCCG ACTGCTCCAG CGGCTGCGAC AGCGACCACT 
     1441 GGGGGCCCCA CTGCAGCAAC CGGTGCCAGT GCCAGAACGG CGCCCTGTGT AACCCCATCA 
     1501 CAGGCGCCTG CGTGTGCGCC GCCGGCTTCC GTGGATGGCG CTGCGAGGAG CTCTGCGCAC 
     1561 CTGGCACCCA CGGCAAGGGA TGCCAGCTGC CGTGCCAGTG CCGACACGGT GCCAGCTGCG 
     1621 ACCCCCGCGC CGGCGAGTGC CTCTGCGCAC CTGGCTACAC CGGCGTCTAC TGCGAGGAGC 
     1681 TGTGCCCTCC TGGGAGCCAT GGAGCTCACT GTGAGCTGCG CTGCCCCTGT CAGAATGGGG 
     1741 GCACCTGCCA CCACATCACT GGCGAGTGTG CCTGCCCCCC AGGCTGGACG GGAGCAGTGT 
     1801 GTGCCCAGCC CTGCCCACCA GGGACATTTG GCCAGAACTG CAGCCAGGAT TGTCCTTGCC 
     1861 ACCATGGAGG GCAGTGTGAC CACGTGACTG GACAGTGCCA CTGTACAGCT GGATACATGG 
     1921 GGGACAGGTG CCAAGAGGAG TGCCCCTTCG GGTCCTTCGG CTTCCAGTGC TCACAGCACT 
     1981 GTGACTGCCA CAATGGGGGG CAGTGTTCAC CCACCACGGG TGCCTGCGAG TGTGAGCCTG 
     2041 GCTACAAGGG CCCACGCTGC CAGGAGCGAC TGTGCCCGGA GGGCCTGCAT GGCCCAGGCT 
     2101 GCACCCTGCC CTGCCCCTGT GACGCTGACA ACACCATCAG CTGCCACCCA GTAACTGGAG 
     2161 CTTGTACCTG CCAGCCAGGC TGGTCTGGTC ACCACTGCAA TGAATCCTGC CCTGTTGGCT 
     2221 ACTATGGCGA TGGCTGCCAG CTGCCTTGCA CCTGTCAGAA TGGCGCCGAC TGCCACAGCA 
     2281 TCACTGGGGG CTGCACTTGT GCTCCGGGCT TCATGGGAGA GGTCTGTGCC GTTTCCTGTG 
     2341 CAGCAGGGAC CTATGGCCCC AACTGCTCGT CCATCTGTAG CTGTAACAAT GGTGGCACCT 
     2401 GCTCCCCAGT AGATGGCTCC TGTACCTGCA AGGAAGGGTG GCAGGGCCTG GACTGCACCC 
     2461 TGCCATGTCC CAGTGGGACG TGGGGCCTGA ACTGCAACGA GAGCTGCACC TGTGCCAATG 
     2521 GGGCAGCCTG CAGCCCCATA GACGGCTCCT GCTCCTGCAC TCCTGGCTGG CTGGGAGACA 
     2581 CCTGTGAGCT GCCTTGCCCG GATGGCACAT TTGGGCTGAA CTGCAGTGAA CACTGTGACT 
     2641 GCAGCCATGC TGATGGATGT GACCCCGTCA CAGGCCACTG CTGCTGCCTG GCCGGATGGA 
     2701 CAGGCATCCG CTGTGACAGC ACGTGTCCAC CTGGCCGCTG GGGCCCCAAC TGCTCTGTCT 
     2761 CCTGCAGCTG TGAGAATGGA GGCTCCTGCT CCCCAGAGGA TGGGAGCTGC GAGTGTGCCC 
     2821 CTGGCTTCCG AGGACCCTTA TGCCAGAGAA TCTGCCCCCC TGGGTTCTAT GGCCACGGCT 
     2881 GCGCCCAGCC ATGCCCCCTC TGCGTGCACA GCAGCAGGCC CTGCCACCAC ATCAGCGGCA 
     2941 TCTGTGAGTG CCTCCCAGGA TTCTCTGGAG CTCTCTGCAA CCAAGTGTGT GCTGGAGGAT 
     3001 ACTTTGGGCA GGACTGTGCC CAGCTCTGCT CCTGTGCCAA CAACGGGACC TGCAGCCCTA 
     3061 TCGATGGCTC CTGCCAGTGC TTTCCTGGAT GGATTGGCAA GGACTGCTCA CAGGCTTGCC 
     3121 CACCCGGGTT CTGGGGCCCC GCCTGCTTCC ACGCATGCAG CTGCCACAAC GGGGCGAGCT 
     3181 GCAGCGCCGA GGACGGGGCC TGCCACTGCA CCCCTGGCTG GACTGGACTC TTCTGCACAC 
     3241 AGCGCTGCCC AGCAGCATTT TTTGGGAAGG ACTGTGGGCG CGTATGCCAG TGTCAGAATG 
     3301 GCGCCAGCTG TGACCACATC AGTGGCAAGT GCACCTGCCG CACAGGCTTC ACCGGGCAAC 
     3361 ACTGTGAGCA GAGATGTGCC CCAGGAACCT TTGGCTATGG GTGTCAGCAG CTATGTGAGT 
     3421 GCATGAACAA CTCCACCTGT GACCATGTCA CCGGCACCTG TTACTGCAGC CCTGGCTTCA 
     3481 AAGGAATCAG GTGTGACCAA GCTGCCCTCA TGATGGAGGA GCTGAATCCC TACACCAAGA 
     3541 TCAGCCCAGC ACTGGGTGCA GAGCGGCACT CGGTGGGTGC TGTCACAGGC ATCATGCTCC 
     3601 TGTTATTCCT CATTGTGGTG CTGCTGGGCC TATTTGCCTG GCATCGGCGG CGGCAGAAAG 
     3661 AGAAGGGCCG AGACCTGGCT CCCCGTGTCT CCTACACACC TGCCATGAGG ATGACCAGCA 
     3721 CCGACTACTC CCTCTCAGGT GCTTGTGGAA TGGATAGACG TCAGAACACA TACATTATGG 
     3781 ACAAAGGCTT CAAAGATTAC ATGAAAGAAT CCGTGTGCAG TTCTAGTACT TGTTCCTTGA 
     3841 ATAGCAGTGA AAACCCTTAC GCCACAATTA AGGACCCACC CATCCTCACC TGCAAGCTTC 
     3901 CAGAAAGCAG CTATGTAGAA ATGAAGTCGC CTGTGCACAT GGGGTCTCCG TACACAGATG 
     3961 TGCCATCCTT GTCGACATCT AATAAAAATA TATATGAAGT TGAGCCCACA GTCAGTGTGG 
     4021 TCCAAGAAGG TTGCGGTCAT AACTCCAGCT ATATCCAGAA TGCATACGAC CTACCTAGGA 
     4081 ACAGCCATAT TCCTGGTCAT TATGACCTCC TCCCAGTAAG ACAGAGCCCT GCCAATGGGC 
     4141 CGTCCCAGGA CAAGCAATCT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4201 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4261 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4321 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4381 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4441 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4501 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4561 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4621 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4681 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4741 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4801 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4861 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4921 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4981 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5041 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5101 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5161 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5221 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5281 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5341 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5401 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5461 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5521 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5581 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5641 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5701 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5761 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5821 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5881 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5941 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6001 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6061 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6121 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6181 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6241 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6301 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6361 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6421 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6481 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6541 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6601 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6661 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6721 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6781 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6841 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6901 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6961 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7021 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7081 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7141 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7201 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7261 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7321 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7381 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7441 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7501 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7561 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7621 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7681 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7741 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7801 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7861 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7921 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7981 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//