LOCUS       RC229371                7218 bp    DNA     circular  SYN 
DEFINITION  MMEL1 (Myc-DDK-tagged)-Human membrane metallo-endopeptidase-like 1 (MMEL1)
ACCESSION   NM_033467
VERSION     NM_033467.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3375..3466 
                     /label="Myc-DDK"  
     misc_feature    3496..4081 
                     /label="PolyA signal"  
     misc_feature    3553..3572 
                     /label="Restriction Enzyme"  
     misc_feature    4357..5029 
                     /label="ColE1"  
     misc_feature    5459..6253 
                     /label="Kan/Neo"  
     misc_feature    6288..6590 
                     /label="SV40 ori"  
     misc_feature    6676..7153 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3357..3363 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3357 
                     /label="MMEL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAGTCC GAAGGCCCAG TGGGGATGGT GGAGAGCGCC GGCCGTGCAG 
     1081 GGCAGAAGCG CCCGGGGTTC CTGGAGGGGG GGCTGCTGCT GCTGCTGCTG CTGGTGACCG 
     1141 CTGCCCTGGT GGCCTTGGGT GTCCTCTACG CCGACCGCAG AGGGAAGCAG CTGCCACGCC 
     1201 TTGCTAGCCG GCTGTGCTTC TTACAGGAGG AGAGGACCTT TGTAAAACGA AAACCCCGAG 
     1261 GGATCCCAGA GGCCCAAGAG GTGAGCGAGG TCTGCACCAC CCCTGGCTGC GTGATAGCAG 
     1321 CTGCCAGGAT CCTCCAGAAC ATGGACCCGA CCACGGAACC GTGTGACGAC TTCTACCAGT 
     1381 TTGCATGCGG AGGCTGGCTG CGGCGCCACG TGATCCCTGA GACCAACTCA AGATACAGCA 
     1441 TCTTTGACGT CCTCCGCGAC GAGCTGGAGG TCATCCTCAA AGCGGTGCTG GAGAATTCGA 
     1501 CTGCCAAGGA CCGGCCGGCT GTGGAGAAGG CCAGGACGCT GTACCGCTCC TGCATGAACC 
     1561 AGAGTGTGAT AGAGAAGCGA GGCTCTCAGC CCCTGCTGGA CATCTTGGAG GTGGTGGGAG 
     1621 GCTGGCCGGT GGCGATGGAC AGGTGGAACG AGACCGTAGG ACTCGAGTGG GAGCTGGAGC 
     1681 GGCAGCTGGC GCTGATGAAC TCACAGTTCA ACAGGCGCGT CCTCATCGAC CTCTTCATCT 
     1741 GGAACGACGA CCAGAACTCC AGCCGGCACA TCATCTACAT AGACCAGCCC ACCTTGGGCA 
     1801 TGCCCTCCCG AGAGTACTAC TTCAACGGCG GCAGCAACCG GAAGGTGCGG GAAGCCTACC 
     1861 TGCAGTTCAT GGTGTCAGTG GCCACGTTGC TGCGGGAGGA TGCAAACCTG CCCAGGGACA 
     1921 GCTGCCTGGT GCAGGAGGAC ATGGTGCAGG TGCTGGAGCT GGAGACACAG CTGGCCAAGG 
     1981 CCACGGTACC CCAGGAGGAG AGACACGACG TCATCGCCTT GTACCACCGG ATGGGACTGG 
     2041 AGGAGCTGCA AAGCCAGTTT GGCCTGAAGG GATTTAACTG GACTCTGTTC ATACAAACTG 
     2101 TGCTATCCTC TGTCAAAATC AAGCTGCTGC CAGATGAGGA AGTGGTGGTC TATGGCATCC 
     2161 CCTACCTGCA GAACCTTGAA AACATCATCG ACACCTACTC AGCCAGGACC ATACAGAACT 
     2221 ACCTGGTCTG GCGCCTGGTG CTGGACCGCA TTGGTAGCCT AAGCCAGAGA TTCAAGGACA 
     2281 CACGAGTGAA CTACCGCAAG GCGCTGTTTG GCACAATGGT GGAGGAGGTG CGCTGGCGTG 
     2341 AATGTGTGGG CTACGTCAAC AGCAACATGG AGAACGCCGT GGGCTCCCTC TACGTCAGGG 
     2401 AGGCGTTCCC TGGAGACAGC AAGAGCATGG TCAGAGAACT CATTGACAAG GTGCGGACAG 
     2461 TGTTTGTGGA GACGCTGGAC GAGCTGGGCT GGATGGACGA GGAGTCCAAG AAGAAGGCGC 
     2521 AGGAGAAGGC CATGAGCATC CGGGAGCAGA TCGGGCACCC TGACTACATC CTGGAGGAGA 
     2581 TGAACAGGCG CCTGGACGAG GAGTACTCCA ATCTGAACTT CTCAGAGGAC CTGTACTTTG 
     2641 AGAACAGTCT GCAGAACCTC AAGGTGGGCG CCCAGCGGAG CCTCAGGAAG CTTCGGGAAA 
     2701 AGGTGGACCC AAATCTCTGG ATCATCGGGG CGGCGGTGGT CAATGCGTTC TACTCCCCAA 
     2761 ACCGAAACCA GATTGTATTC CCTGCCGGGA TCCTCCAGCC CCCCTTCTTC AGCAAGGAGC 
     2821 AGCCACAGGC CTTGAACTTT GGAGGCATTG GGATGGTGAT CGGGCACGAG ATCACGCACG 
     2881 GCTTTGACGA CAATGGCCGG AACTTCGACA AGAATGGCAA CATGATGGAT TGGTGGAGTA 
     2941 ACTTCTCCAC CCAGCACTTC CGGGAGCAGT CAGAGTGCAT GATCTACCAG TACGGCAACT 
     3001 ACTCCTGGGA CCTGGCAGAC GAACAGAACG TGAACGGATT CAACACCCTT GGGGAAAACA 
     3061 TTGCTGACAA CGGAGGGGTG CGGCAAGCCT ATAAGGCCTA CCTCAAGTGG ATGGCAGAGG 
     3121 GTGGCAAGGA CCAGCAGCTG CCCGGCCTGG ATCTCACCCA TGAGCAGCTC TTCTTCATCA 
     3181 ACTATGCCCA GGTGTGGTGC GGGTCCTACC GGCCCGAGTT CGCCATCCAA TCCATCAAGA 
     3241 CAGACGTCCA CAGTCCCCTG AAGTACAGGG TACTGGGGTC GCTGCAGAAC CTGGCCGCCT 
     3301 TCGCAGACAC GTTCCACTGT GCCCGGGGCA CCCCCATGCA CCCCAAGGAG CGATGCCGCG 
     3361 TGTGGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3421 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3481 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3541 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3601 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3661 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3721 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3781 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3841 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3901 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3961 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4021 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4081 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4141 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4201 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4261 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4321 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4381 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4441 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4501 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4561 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4621 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4681 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4741 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4801 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4861 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4921 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4981 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5041 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5101 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5161 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5221 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5281 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5341 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5401 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5461 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5521 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5581 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5641 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5701 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5761 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5821 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5881 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5941 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6001 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6061 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6121 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6181 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6241 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6301 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6361 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6421 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6481 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6541 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6601 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6661 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6721 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6781 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6841 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6901 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6961 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7021 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7081 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7141 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7201 AAATTTAACG CGAATTTT
//