LOCUS       RC229079                9327 bp    DNA     circular  SYN 
DEFINITION  KDM5D (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 5D (KDM5D), transcript variant 3
ACCESSION   NM_001146706
VERSION     NM_001146706.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5484..5575 
                     /label="Myc-DDK"  
     misc_feature    5605..6190 
                     /label="PolyA signal"  
     misc_feature    5662..5681 
                     /label="Restriction Enzyme"  
     misc_feature    6466..7138 
                     /label="ColE1"  
     misc_feature    7568..8362 
                     /label="Kan/Neo"  
     misc_feature    8397..8699 
                     /label="SV40 ori"  
     misc_feature    8785..9262 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5466..5472 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5466 
                     /label="KDM5D"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACCGGGG TGTGACGAGT TCCTGCCGCC ACCGGAGTGC CCGGTTTTTG 
     1081 AGCCTAGCTG GGCTGAATTC CAAGACCCGC TTGGCTACAT TGCGAAAATA AGGCCCATAG 
     1141 CAGAGAAGTC TGGCATCTGC AAAATCCGCC CACCCGCGGA TTGGCAGCCT CCTTTTGCAG 
     1201 TAGAAGTTGA CAATTTCAGA TTTACTCCTC GCGTCCAAAG GCTAAATGAA CTGGAGGCCC 
     1261 AAACTAGAGT GAAATTGAAC TATTTGGATC AGATTGCAAA ATTCTGGGAA ATTCAAGGCT 
     1321 CCTCTTTAAA GATTCCCAAT GTGGAGCGGA AGATCTTGGA CCTCTACAGC CTTAGTAAGC 
     1381 AATGTAACAC ACACCCGTTT GACAATGAGG TAAAAGATAA GGAATACAAG CCCCACAGCA 
     1441 TCCCCCTTAG ACAGTCTGTG CAGCCTTCAA AGTTCAGCAG CTACAGTCGA CGGGCAAAAA 
     1501 GGCTACAGCC TGATCCAGAG CCTACAGAGG AGGACATTGA GAAGCATCCA GAGCTAAAGA 
     1561 AGTTACAGAT ATATGGGCCA GGTCCCAAAA TGATGGGCTT GGGCCTTATG GCTAAGGATA 
     1621 AGGATAAGAC TGTGCATAAG AAAGTCACAT GCCCCCCAAC TGTTACGGTG AAGGATGAGC 
     1681 AAAGTGGAGG TGGGAACGTG TCATCAACAT TGCTCAAGCA GCACTTGAGC CTAGAGCCCT 
     1741 GCACTAAGAC AACCATGCAA CTTCGAAAGA ATCACAGCAG TGCCCAGTTT ATTGACTCAT 
     1801 ATATTTGCCA AGTATGCTCC CGTGGGGATG AAGATGATAA GCTTCTTTTC TGTGATGGCT 
     1861 GTGATGACAA TTACCACATC TTCTGCTTGT TACCACCCCT TCCTGAAATC CCCAGAGGCA 
     1921 TCTGGAGGTG CCCAAAATGT ATCTTGGCGG AGTGTAAACA GCCTCCTGAA GCTTTTGGAT 
     1981 TTGAACAGGC TACCCAGGAG TACAGTTTGC AGAGTTTTGG TGAAATGGCT GATTCCTTCA 
     2041 AGTCCGACTA CTTCAACATG CCTGTACATA TGGTGCCTAC AGAACTTGTA GAGAAGGAAT 
     2101 TCTGGAGGCT GGTGAGCAGC ATTGAGGAAG ACGTGACAGT TGAATATGGA GCTGATATTC 
     2161 ATTCCAAAGA ATTTGGCAGT GGCTTTCCTG TCAGCAATAG CAAACAAAAC TTATCTCCTG 
     2221 AGGAGAAGGA GTATGCGACC AGTGGTTGGA ACCTGAATGT GATGCCAGTG CTAGATCAGT 
     2281 CTGTTCTCTG TCACATCAAT GCAGACATCT CAGGCATGAA GGTGCCCTGG CTGTACGTGG 
     2341 GCATGGTTTT CTCAGCATTT TGTTGGCATA TTGAGGATCA CTGGAGTTAC TCTATTAACT 
     2401 ATCTGCATTG GGGTGAGCCG AAGACCTGGT ATGGTGTACC CTCCCTGGCA GCAGAGCATT 
     2461 TGGAGGAGGT GATGAAGATG CTGACACCTG AGCTGTTTGA TAGCCAGCCT GATCTCCTAC 
     2521 ACCAGCTTGT CACTCTCATG AATCCCAACA CTTTGATGTC CCATGGTGTG CCAGTTGTCC 
     2581 GCACAAACCA GTGTGCAGGG GAGTTTGTCA TCACTTTTCC TCGTGCTTAC CACAGTGGTT 
     2641 TTAACCAAGG CTACAATTTT GCTGAAGCTG TCAACTTTTG TACTGCTGAC TGGCTACCTG 
     2701 CTGGACGCCA GTGCATTGAA CACTACCGCC GGCTCCGGCG CTATTGTGTC TTCTCCCACG 
     2761 AGGAGCTCAT CTGCAAGATG GCTGCCTTCC CAGAGACGTT GGATCTCAAT CTAGCAGTAG 
     2821 CTGTGCACAA GGAGATGTTC ATTATGGTTC AGGAGGAGCG ACGTCTACGA AAGGCCCTTT 
     2881 TGGAGAAGGG CGTCACGGAG GCTGAGCGAG AGGCTTTTGA GCTGCTCCCA GATGATGAAC 
     2941 GCCAGTGCAT CAAGTGCAAG ACCACGTGCT TCTTGTCAGC CCTGGCCTGC TACGACTGCC 
     3001 CAGATGGCCT TGTATGCCTT TCCCACATCA ATGACCTCTG CAAGTGCTCT AGTAGCCGAC 
     3061 AGTACCTCCG GTATCGGTAC ACCTTGGATG AGCTCCCCAC CATGCTGCAT AAACTGAAGA 
     3121 TTCGGGCTGA GTCTTTTGAC ACCTGGGCCA ACAAAGTGCG AGTGGCCTTG GAGGTGGAGG 
     3181 ATGGCCGTAA ACGCAGCTTT GAAGAGCTAA GGGCACTGGA GTCTGAGGCT CGTGAGAGGA 
     3241 GGTTTCCTAA TAGTGAGCTG CTTCAGCGAC TGAAGAACTG CCTGAGTGAG GTGGAGGCTT 
     3301 GTATTGCTCA AGTCCTGGGG CTGGTCAGTG GTCAGGTGGC CAGGATGGAC ACTCCACAGC 
     3361 TGACCTTGAC TGAACTCCGG GTCCTTCTTG AGCAGATGGG CAGCCTGCCC TGTGCCATGC 
     3421 ATCAGATTGG GGATGTCAAG GATGTCCTGG AACAGGTGGA GGCCTATCAA GCTGAGGCTC 
     3481 GTGAGGCTCT GGCCACACTG CCCTCTAGTC CAGGGCTATT GCGGTCCCTG TTGGAGAGGG 
     3541 GGCAGCAGCT GGGTGTAGAG GTGCCTGAAG CCCATCAGCT TCAGCAGCAG GTGGAGCAGG 
     3601 CGCAATGGCT AGATGAAGTG AAGCAGGCCC TGGCCCCTTC TGCTCACAGG GGCTCTCTGG 
     3661 TCATCATGCA GGGGCTTTTG GTTATGGGTG CCAAGATAGC CTCCAGCCCT TCTGTGGACA 
     3721 AGGCCCGGGC TGAGCTGCAA GAACTACTGA CCATTGCAGA GCGCTGGGAA GAAAAGGCTC 
     3781 ATTTCTGCCT GGAGGCCAGG CAGAAGCATC CACCAGCCAC ATTGGAAGCC ATAATTCGTG 
     3841 AGACAGAAAA CATCCCTGTT CACCTGCCTA ACATCCAGGC TCTCAAAGAA GCTCTGACTA 
     3901 AGGCACAAGC TTGGATTGCT GATGTGGATG AGATCCAAAA TGGTGACCAC TACCCCTGTC 
     3961 TAGATGACTT GGAGGGCCTG GTGGCTGTGG GCCGGGACCT GCCTGTGGGG CTGGAAGAGC 
     4021 TGAGACAGCT AGAGCTGCAG GTATTGACAG CACATTCCTG GAGAGAGAAG GCCTCCAAGA 
     4081 CCTTTCTCAA GAAGAATTCT TGCTACACAC TGCTTGAGGT GCTTTGCCCG TGTGCAGACG 
     4141 CTGGCTCAGA CAGCACCAAG CGTAGCCGGT GGATGGAGAA GGCGCTGGGG TTGTACCAGT 
     4201 GTGACACAGA GCTGCTGGGG CTGTCTGCAC AGGACCTCAG AGACCCAGGC TCTGTGATTG 
     4261 TGGCCTTCAA GGAAGGGGAA CAGAAGGAGA AGGAGGGTAT CCTGCAGCTG CGTCGCACCA 
     4321 ACTCAGCCAA GCCCAGTCCA CTGGCACCAT CCCTCATGGC CTCTTCTCCG ACTTCTATCT 
     4381 GTGTGTGTGG GCAGGTGCCA GCTGGGGTGG GAGTTCTGCA GTGTGACCTG TGTCAGGACT 
     4441 GGTTCCATGG GCAGTGTGTG TCAGTGCCCC ATCTCCTCAC CTCTCCAAAG CCCAGTCTCA 
     4501 CTTCATCTCC ACTGCTAGCC TGGTGGGAAT GGGACACAAA ATTCCTGTGT CCACTGTGTA 
     4561 TGCGCTCACG ACGGCCACGC CTAGAGACAA TCCTAGCCTT GCTGGTTGCC CTGCAGAGGC 
     4621 TGCCCGTGCG GCTGCCTGAG GGTGAGGCCC TTCAGTGTCT CACAGAGAGG GCCATTGGCT 
     4681 GGCAAGACCG TGCCAGAAAG GCTCTGGCCT CTGAAGATGT GACTGCTCTG TTGCGACAGC 
     4741 TGGCTGAGCT TCGCCAACAG CTACAGGCCA AACCCAGACC AGAGGAGGCC TCAGTCTACA 
     4801 CTTCAGCCAC TGCCTGTGAC CCTATCAGAG AAGGCAGTGG CAACAATATT TCTAAGGTCC 
     4861 AAGGGCTGCT GGAGAATGGA GACAGTGTGA CCAGTCCTGA GAACATGGCT CCAGGAAAGG 
     4921 GCTCTGACCT GGAGCTACTG TCCTCACTGT TGCCGCAGTT GACTGGCCCT GTGTTGGAGC 
     4981 TGCCTGAGGC AATCCGGGCT CCCCTGGAGG AGCTCATGAT GGAAGGGGAC CTGCTTGAGG 
     5041 TGACCCTGGA TGAGAACCAC AGCATCTGGC AGCTGCTGCA GGCTGGACAG CCTCCAGACC 
     5101 TGGACAGAAT TCGCACACTT CTGGAGCTGG AAAAATTTGA ACATCAAGGG AGTCGGACAA 
     5161 GGAGCCGGGC TCTGGAGAGG CGACGGCGGC GGCAGAAGGT GGATCAGGGT AGAAACGTTG 
     5221 AGAATCTTGT TCAACAGGAG CTTCAGTCAA AAAGGGCTCG GAGCTCAGGG ATTATGTCTC 
     5281 AGGTGGGCCG AGAAGAAGAA CATTATCAGG AGAAAGCAGA CCGTGAAAAT ATGTTCCTGA 
     5341 CACCTTCCAC AGACCACAGC CCTTTCTTGA AAGGAAACCA AAATAGCTTA CAACACAAGG 
     5401 ATTCAGGCTC TTCAGCTGCT TGTCCTTCTT TAATGCCTTT GCTACAACTC TCCTACTCTG 
     5461 ATGAGCAACA GTTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5521 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5581 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5641 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5701 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5761 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5821 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5881 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5941 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     6001 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6061 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6121 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6181 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6241 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6301 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6361 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6421 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6481 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6541 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6601 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6661 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6721 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6781 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6841 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6901 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6961 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     7021 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7081 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7141 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7201 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7261 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7321 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7381 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7441 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7501 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7561 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7621 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7681 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7741 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7801 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7861 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7921 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7981 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     8041 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8101 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8161 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8221 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8281 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8341 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8401 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8461 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8521 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8581 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8641 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8701 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8761 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8821 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8881 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8941 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     9001 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9061 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9121 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9181 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9241 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9301 ATTTAACAAA AATTTAACGC GAATTTT
//