LOCUS       RC229078                9273 bp    DNA     circular  SYN 
DEFINITION  PEG3 (Myc-DDK-tagged)-Human paternally expressed 3 (PEG3), transcript variant 5
ACCESSION   NM_001146187
VERSION     NM_001146187.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5430..5521 
                     /label="Myc-DDK"  
     misc_feature    5551..6136 
                     /label="PolyA signal"  
     misc_feature    5608..5627 
                     /label="Restriction Enzyme"  
     misc_feature    6412..7084 
                     /label="ColE1"  
     misc_feature    7514..8308 
                     /label="Kan/Neo"  
     misc_feature    8343..8645 
                     /label="SV40 ori"  
     misc_feature    8731..9208 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5412..5418 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5412 
                     /label="PEG3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACCAACCA GAAGACGACA ACAACAGTGA CGTGACCAGC GACGACGACA 
     1081 TGACCCGGAA CAGAAGAGAG TCCTCACCAC CTCACTCAGT CCATTCTTTC AGTGGTGACC 
     1141 GGGACTGGGA CCGGAGGGGC AGAAGCAGAG ACATGGAGCC ACGAGACCGC TGGTCCCACA 
     1201 CCAGGAACCC AAGAAGCAGG ATGCCTCCGC GGGATCTTTC CCTTCCTGTG GTGGCGAAAA 
     1261 CAAGCTTTGA AATGGACAGA GAGGACGACA GGGACTCCAG GGCTTATGAG TCCCGATCTC 
     1321 AGGATGCTGA ATCATACCAA AATGTGGTGG ACCTCGCTGA GGACAGGAAA CCTCACAACA 
     1381 CAATCCAGGA CAACATGGAA AACTACAGGA AGCTGCTCTC CCTCGGAGTG CAGCTTGCTG 
     1441 AAGACGATGG CCACTCCCAC ATGACGCAGG GCCACTCATC AAGATCCAAG AGAAGTGCCT 
     1501 ACCCAAGCAC CAGTCGAGGT CTAAAAACTA TGCCTGAAGC CAAAAAATCA ACCCACCGGC 
     1561 GGGGGATTTG TGAAGATGAA TCTTCCCACG GAGTGATAAT GGAAAAATTC ATCAAGGATG 
     1621 TGTCACGCAG TTCCAAATCG GGAAGAGCAA GGGAGTCAAG CGACCGGTCA CAGAGATTCC 
     1681 CCAGAATGTC AGATGATAAC TGGAAGGACA TTTCATTGAA CAAGAGGGAG TCAGTGATCC 
     1741 AGCAGCGGGT TTATGAAGGG AATGCATTTA GGGGAGGCTT TAGGTTTAAT TCAACCCTTG 
     1801 TTTCCAGAAA GAGAGTTCTT GAAAGAAAGA GGCGCTATCA TTTTGACACA GATGGGAAGG 
     1861 GCTCGATTCA CGATCAAAAA GGCTGTCCCA GGAAGAAGCC CTTTGAATGT GGTAGTGAGA 
     1921 TGAGAAAAGC CATGAGCGTG AGCAGCCTGA GCAGCCTCAG CTCCCCCTCC TTTACCGAGT 
     1981 CACAGCCAAT TGATTTTGGG GCAATGCCAT ATGTATGTGA TGAGTGTGGG AGGTCGTTCA 
     2041 GTGTCATCTC AGAATTTGTT GAGCACCAGA TCATGCATAC TAGAGAGAAC CTCTATGAGT 
     2101 ATGGTGAGTC CTTTATCCAC AGTGTGGCTG TCAGTGAAGT TCAGAAAAGT CAGGTTGGAG 
     2161 GGAAACGTTT TGAATGTAAG GACTGTGGAG AGACCTTCAA TAAGAGTGCC GCCTTGGCTG 
     2221 AACATCGGAA GATTCATGCT AGAGGTTATC TTGTGGAATG TAAGAATCAG GAATGTGAGG 
     2281 AAGCCTTCAT GCCTAGCCCC ACCTTTAGTG AGCTTCAGAA AATATATGGC AAAGACAAAT 
     2341 TCTACGAGTG CAGGGTGTGT AAGGAAACCT TCCTTCATAG TTCTGCCCTG ATTGAGCACC 
     2401 AGAAAATCCA CTTTGGGGAT GACAAAGATA ATGAGCGTGA ACATGAACGT GAACGTGAAC 
     2461 GTGAGCGCGG GGAAACCTTT AGGCCCAGCC CAGCCCTTAA TGAGTTTCAG AAAATGTATG 
     2521 GTAAAGAGAA AATGTACGAA TGTAAGGTGT GTGGGGAGAC TTTCCTTCAT AGCTCATCCC 
     2581 TGAAAGAACA TCAGAAAATC CATACTAGAG GGAACCCATT TGAAAACAAG GGTAAAGTGT 
     2641 GTGAGGAAAC CTTTATTCCT GGTCAGTCCC TTAAAAGGCG TCAGAAAACT TACAATAAGG 
     2701 AGAAGCTCTG TGACTTTACA GATGGCCGGG ATGCCTTCAT GCAAAGCTCA GAGCTCAGTG 
     2761 AGCATCAGAA AATTCATTCT CGAAAGAACC TCTTTGAAGG CAGAGGGTAT GAGAAATCTG 
     2821 TCATTCATAG TGGGCCATTC ACTGAATCTC AGAAGAGTCA TACTATAACA AGACCTCTTG 
     2881 AAAGTGATGA GGACGAAAAG GCGTTCACCA TTAGCTCTAA CCCCTATGAA AACCAGAAGA 
     2941 TTCCCACTAA GGAAAATGTC TACGAGGCAA AATCATATGA GAGGTCTGTT ATTCATAGCT 
     3001 TAGCCTCTGT GGAAGCTCAG AAAAGTCACA GTGTAGCAGG GCCCAGTAAA CCAAAAGTAA 
     3061 TGGCAGAGTC TACCATTCAG AGCTTCGATG CTATCAACCA TCAGAGAGTT CGTGCTGGAG 
     3121 GGAACACCTC TGAAGGAAGG GAATACAGTA GGTCTGTTAT CCATAGCTTA GTGGCTTCCA 
     3181 AACCTCCAAG AAGTCACAAT GGAAATGAAT TGGTGGAATC TAATGAGAAG GGAGAATCCT 
     3241 CCATTTATAT CTCAGACCTT AATGATAAGC GACAGAAGAT TCCTGCCAGA GAGAACCCTT 
     3301 GTGAAGGGGG CAGTAAGAAT CGCAACTATG AAGACTCTGT CATACAGAGT GTATTCCGTG 
     3361 CCAAACCTCA GAAAAGTGTT CCTGGAGAGG GATCTGGTGA GTTTAAGAAG GATGGCGAAT 
     3421 TCTCTGTTCC CAGCTCAAAT GTCCGTGAAT ACCAGAAGGC TCGTGCTAAA AAGAAATACA 
     3481 TTGAGCATAG GAGCAATGAG ACCTCTGTAA TTCACTCTCT GCCTTTTGGT GAACAAACAT 
     3541 TTCGCCCTCG AGGGATGCTC TATGAATGTC AGGAGTGTGG GGAGTGCTTT GCTCATAGCT 
     3601 CTGACCTCAC TGAGCACCAG AAGATTCATG ATAGGGAGAA GCCCTCTGGA AGCAGAAACT 
     3661 ATGAATGGTC TGTCATTCGC AGCTTGGCCC CTACTGACCC TCAAACAAGT TACGCCCAAG 
     3721 AGCAGTATGC TAAAGAGCAA GCGCGGAACA AATGTAAGGA CTTCAGACAA TTTTTTGCTA 
     3781 CCAGCGAAGA CCTCAACACA AACCAGAAAA TCTATGACCA AGAGAAGTCT CATGGCGAGG 
     3841 AGTCTCAAGG CGAGAATACT GATGGGGAGG AGACCCACAG CGAGGAGACC CATGGTCAGG 
     3901 AGACAATTGA AGACCCTGTC ATTCAAGGCT CAGACATGGA AGACCCTCAG AAGGATGACC 
     3961 CTGATGACAA AATCTATGAA TGTGAGGACT GTGGCCTGGG CTTTGTGGAT CTCACAGACC 
     4021 TCACAGACCA TCAGAAAGTC CACAGCAGGA AGTGCCTGGT TGACAGTCGG GAGTACACAC 
     4081 ATTCTGTAAT TCACACCCAT TCCATCAGCG AGTATCAGAG AGATTACACT GGAGAGCAGC 
     4141 TGTATGAATG TCCAAAGTGT GGGGAATCTT TTATTCATAG CTCATTCCTT TTCGAGCATC 
     4201 AGAGAATCCA TGAACAAGAC CAGTTGTATT CCATGAAGGG GTGTGATGAT GGTTTTATTG 
     4261 CCCTCTTGCC CATGAAGCCA CGGAGGAATC GTGCTGCAGA GAGGAATCCT GCTCTTGCTG 
     4321 GGTCGGCCAT TCGATGCCTT TTGTGTGGAC AAGGCTTCAT TCATAGCTCT GCCCTTAATG 
     4381 AGCATATGAG ACTTCATAGG GAAGATGATT TACTGGAGCA GAGCCAGATG GCTGAGGAAG 
     4441 CTATCATTCC AGGCTTAGCC CTCACTGAGT TTCAGAGAAG TCAGACCGAA GAGAGACTCT 
     4501 TTGAATGTGC AGTCTGTGGA GAATCTTTCG TCAACCCAGC AGAACTTGCA GATCACGTAA 
     4561 CTGTTCATAA GAATGAGCCC TATGAGTACG GGTCCTCCTA TACTCACACC TCATTTCTTA 
     4621 CTGAGCCCCT CAAAGGAGCT ATACCATTCT ATGAATGCAA GGATTGTGGT AAGTCCTTTA 
     4681 TTCATAGCAC AGTCCTCACT AAACATAAGG AGCTTCATCT GGAAGAAGAA GAAGAAGATG 
     4741 AAGCAGCAGC AGCTGCAGCA GCAGCAGCCC AGGAAGTTGA AGCCAATGTC CATGTTCCAC 
     4801 AAGTAGTTCT GAGGATTCAG GGCTTAAACG TAGAGGCTGC TGAGCCAGAA GTGGAGGCTG 
     4861 CCGAGCCAGA AGTGGAGGCT GCTGAGCCAG AAGTGGAGGC TGCTGAGCCA AACGGAGAGG 
     4921 CTGAAGGGCC AGATGGAGAG GCTGCAGAGC CCATTGGAGA GGCTGGACAG CCAAATGGAG 
     4981 AGGCCGAGCA GCCAAATGGG GATGCTGATG AGCCAGATGG TGCAGGTATT GAAGACCCAG 
     5041 AAGAAAGAGC TGAAGAGCCA GAGGGAAAAG CTGAAGAGCC AGAGGGAGAT GCCGACGAGC 
     5101 CTGACGGTGT GGGAATTGAA GACCCAGAAG AAGGTGAAGA TCAAGAGATT CAGGTAGAAG 
     5161 AACCATACTA TGACTGCCAT GAATGCACAG AAACCTTCAC TTCCAGCACA GCATTCAGTG 
     5221 AACACCTGAA AACTCATGCC AGCATGATCA TATTTGAGCC TGCAAATGCC TTTGGGGAGT 
     5281 GCTCAGGCTA CATCGAACGT GCCAGCACCA GCACAGGTGG TGCCAATCAA GCTGATGAGA 
     5341 AGTACTTCAA ATGTGACGTC TGTGGGCAGC TCTTCAATGA CCGCCTGTCC CTCGCCAGAC 
     5401 ACCAGAATAC CCACACTGGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5461 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5521 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5581 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5641 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5701 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5761 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5821 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5881 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5941 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6001 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6061 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6121 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6181 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6241 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6301 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6361 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6421 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6481 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6541 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6601 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6661 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6721 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6781 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6841 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6901 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6961 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7021 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7081 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7141 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7201 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7261 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7321 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7381 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7441 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7501 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7561 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7621 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7681 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7741 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7801 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7861 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7921 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7981 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8041 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8101 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8161 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8221 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8281 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8341 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8401 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8461 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8521 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8581 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8641 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8701 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8761 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8821 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8881 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8941 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9001 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9061 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9121 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9181 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9241 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//