LOCUS       RC229077                9267 bp    DNA     circular  SYN 
DEFINITION  PEG3 (Myc-DDK-tagged)-Human paternally expressed 3 (PEG3), transcript variant 3
ACCESSION   NM_001146185
VERSION     NM_001146185.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5424..5515 
                     /label="Myc-DDK"  
     misc_feature    5545..6130 
                     /label="PolyA signal"  
     misc_feature    5602..5621 
                     /label="Restriction Enzyme"  
     misc_feature    6406..7078 
                     /label="ColE1"  
     misc_feature    7508..8302 
                     /label="Kan/Neo"  
     misc_feature    8337..8639 
                     /label="SV40 ori"  
     misc_feature    8725..9202 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5406..5412 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5406 
                     /label="PEG3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACCAACCA GAAGACGACA ACAACAGTGA CGTGACCAGC GACGACGACA 
     1081 TGACCCGGAA CAGAAGAGAG TCCTCACCAC CTCACTCAGT CCATTCTTTC AGTGACCGGG 
     1141 ACTGGGACCG GAGGGGCAGA AGCAGAGACA TGGAGCCACG AGACCGCTGG TCCCACACCA 
     1201 GGAACCCAAG AAGCAGGATG CCTCCGCGGG ATCTTTCCCT TCCTGTGGTG GCGAAAACAA 
     1261 GCTTTGAAAT GGACAGAGAG GACGACAGGG ACTCCAGGGC TTATGAGTCC CGATCTCAGG 
     1321 ATGCTGAATC ATACCAAAAT GTGGTGGACC TCGCTGAGGA CAGGAAACCT CACAACACAA 
     1381 TCCAGGACAA CATGGAAAAC TACAGGAAGC TGCTCTCCCT CGTGCAGCTT GCTGAAGACG 
     1441 ATGGCCACTC CCACATGACG CAGGGCCACT CATCAAGATC CAAGAGAAGT GCCTACCCAA 
     1501 GCACCAGTCG AGGTCTAAAA ACTATGCCTG AAGCCAAAAA ATCAACCCAC CGGCGGGGGA 
     1561 TTTGTGAAGA TGAATCTTCC CACGGAGTGA TAATGGAAAA ATTCATCAAG GATGTGTCAC 
     1621 GCAGTTCCAA ATCGGGAAGA GCAAGGGAGT CAAGCGACCG GTCACAGAGA TTCCCCAGAA 
     1681 TGTCAGATGA TAACTGGAAG GACATTTCAT TGAACAAGAG GGAGTCAGTG ATCCAGCAGC 
     1741 GGGTTTATGA AGGGAATGCA TTTAGGGGAG GCTTTAGGTT TAATTCAACC CTTGTTTCCA 
     1801 GAAAGAGAGT TCTTGAAAGA AAGAGGCGCT ATCATTTTGA CACAGATGGG AAGGGCTCGA 
     1861 TTCACGATCA AAAAGGCTGT CCCAGGAAGA AGCCCTTTGA ATGTGGTAGT GAGATGAGAA 
     1921 AAGCCATGAG CGTGAGCAGC CTGAGCAGCC TCAGCTCCCC CTCCTTTACC GAGTCACAGC 
     1981 CAATTGATTT TGGGGCAATG CCATATGTAT GTGATGAGTG TGGGAGGTCG TTCAGTGTCA 
     2041 TCTCAGAATT TGTTGAGCAC CAGATCATGC ATACTAGAGA GAACCTCTAT GAGTATGGTG 
     2101 AGTCCTTTAT CCACAGTGTG GCTGTCAGTG AAGTTCAGAA AAGTCAGGTT GGAGGGAAAC 
     2161 GTTTTGAATG TAAGGACTGT GGAGAGACCT TCAATAAGAG TGCCGCCTTG GCTGAACATC 
     2221 GGAAGATTCA TGCTAGAGGT TATCTTGTGG AATGTAAGAA TCAGGAATGT GAGGAAGCCT 
     2281 TCATGCCTAG CCCCACCTTT AGTGAGCTTC AGAAAATATA TGGCAAAGAC AAATTCTACG 
     2341 AGTGCAGGGT GTGTAAGGAA ACCTTCCTTC ATAGTTCTGC CCTGATTGAG CACCAGAAAA 
     2401 TCCACTTTGG GGATGACAAA GATAATGAGC GTGAACATGA ACGTGAACGT GAACGTGAGC 
     2461 GCGGGGAAAC CTTTAGGCCC AGCCCAGCCC TTAATGAGTT TCAGAAAATG TATGGTAAAG 
     2521 AGAAAATGTA CGAATGTAAG GTGTGTGGGG AGACTTTCCT TCATAGCTCA TCCCTGAAAG 
     2581 AACATCAGAA AATCCATACT AGAGGGAACC CATTTGAAAA CAAGGGTAAA GTGTGTGAGG 
     2641 AAACCTTTAT TCCTGGTCAG TCCCTTAAAA GGCGTCAGAA AACTTACAAT AAGGAGAAGC 
     2701 TCTGTGACTT TACAGATGGC CGGGATGCCT TCATGCAAAG CTCAGAGCTC AGTGAGCATC 
     2761 AGAAAATTCA TTCTCGAAAG AACCTCTTTG AAGGCAGAGG GTATGAGAAA TCTGTCATTC 
     2821 ATAGTGGGCC ATTCACTGAA TCTCAGAAGA GTCATACTAT AACAAGACCT CTTGAAAGTG 
     2881 ATGAGGACGA AAAGGCGTTC ACCATTAGCT CTAACCCCTA TGAAAACCAG AAGATTCCCA 
     2941 CTAAGGAAAA TGTCTATGAG GCAAAATCAT ATGAGAGGTC TGTTATTCAT AGCTTAGCCT 
     3001 CTGTGGAAGC TCAGAAAAGT CACAGTGTAG CAGGGCCCAG TAAACCAAAA GTAATGGCAG 
     3061 AGTCTACCAT TCAGAGCTTC GATGCTATCA ACCATCAGAG AGTTCGTGCT GGAGGGAACA 
     3121 CCTCTGAAGG AAGGGAATAC AGTAGGTCTG TTATCCATAG CTTAGTGGCT TCCAAACCTC 
     3181 CAAGAAGTCA CAATGGAAAT GAATTGGTGG AATCTAATGA GAAGGGAGAA TCCTCCATTT 
     3241 ATATCTCAGA CCTTAATGAT AAGCGACAGA AGATTCCTGC CAGAGAGAAC CCTTGTGAAG 
     3301 GGGGCAGTAA GAATCGCAAC TATGAAGACT CTGTCATACA GAGTGTATTC CGTGCCAAAC 
     3361 CTCAGAAAAG TGTTCCTGGA GAGGGATCTG GTGAGTTTAA GAAGGATGGC GAATTCTCTG 
     3421 TTCCCAGCTC AAATGTCCGT GAATACCAGA AGGCTCGTGC TAAAAAGAAA TACATTGAGC 
     3481 ATAGGAGCAA TGAGACCTCT GTAATTCACT CTCTGCCTTT TGGTGAACAA ACATTTCGCC 
     3541 CTCGAGGGAT GCTCTATGAA TGTCAGGAGT GTGGGGAGTG CTTTGCTCAT AGCTCTGACC 
     3601 TCACTGAGCA CCAGAAGATT CATGATAGGG AGAAGCCCTC TGGAAGCAGA AACTATGAAT 
     3661 GGTCTGTCAT TCGCAGCTTG GCCCCTACTG ACCCTCAAAC AAGTTACGCC CAAGAGCAGT 
     3721 ATGCTAAAGA GCAAGCGCGG AACAAATGTA AGGACTTCAG ACAATTTTTT GCTACCAGCG 
     3781 AAGACCTCAA CACAAACCAG AAAATCTATG ACCAAGAGAA GTCTCATGGC GAGGAGTCTC 
     3841 AAGGCGAGAA TACTGATGGG GAGGAGACCC ACAGCGAGGA GACCCATGGT CAGGAGACAA 
     3901 TTGAAGACCC TGTCATTCAA GGCTCAGACA TGGAAGACCC TCAGAAGGAT GACCCTGATG 
     3961 ACAAAATCTA TGAATGTGAG GACTGTGGCC TGGGCTTTGT GGATCTCACA GACCTCACAG 
     4021 ACCATCAGAA AGTCCACAGC AGGAAGTGCC TGGTTGACAG TCGGGAGTAC ACACATTCTG 
     4081 TAATTCACAC CCATTCCATC AGCGAGTATC AGAGAGATTA CACTGGAGAG CAGCTGTATG 
     4141 AATGTCCAAA GTGTGGGGAA TCTTTTATTC ATAGCTCATT CCTTTTCGAG CATCAGAGAA 
     4201 TCCATGAACA AGACCAGTTG TATTCCATGA AGGGGTGTGA TGATGGTTTT ATTGCCCTCT 
     4261 TGCCCATGAA GCCACGGAGG AATCGTGCTG CAGAGAGGAA TCCTGCTCTT GCTGGGTCGG 
     4321 CCATTCGATG CCTTTTGTGT GGACAAGGCT TCATTCATAG CTCTGCCCTT AATGAGCATA 
     4381 TGAGACTTCA TAGGGAAGAT GATTTACTGG AGCAGAGCCA GATGGCTGAG GAAGCTATCA 
     4441 TTCCAGGCTT AGCCCTCACT GAGTTTCAGA GAAGTCAGAC CGAAGAGAGA CTCTTTGAAT 
     4501 GTGCAGTCTG TGGAGAATCT TTCGTCAACC CAGCAGAACT TGCAGATCAC GTAACTGTTC 
     4561 ATAAGAATGA GCCCTATGAG TACGGGTCCT CCTATACTCA CACCTCATTT CTTACTGAGC 
     4621 CCCTCAAAGG AGCTATACCA TTCTATGAAT GCAAGGATTG TGGTAAGTCC TTTATTCATA 
     4681 GCACAGTCCT CACTAAACAT AAGGAGCTTC ATCTGGAAGA AGAAGAAGAA GATGAAGCAG 
     4741 CAGCAGCTGC AGCAGCAGCA GCCCAGGAAG TTGAAGCCAA TGTCCATGTT CCACAAGTAG 
     4801 TTCTGAGGAT TCAGGGCTTA AACGTAGAGG CTGCTGAGCC AGAAGTGGAG GCTGCCGAGC 
     4861 CAGAAGTGGA GGCTGCTGAG CCAGAAGTGG AGGCTGCTGA GCCAAACGGA GAGGCTGAAG 
     4921 GGCCAGATGG AGAGGCTGCA GAGCCCATTG GAGAGGCTGG ACAGCCAAAT GGAGAGGCCG 
     4981 AGCAGCCAAA TGGGGATGCT GATGAGCCAG ATGGTGCAGG TATTGAAGAC CCAGAAGAAA 
     5041 GAGCTGAAGA GCCAGAGGGA AAAGCTGAAG AGCCAGAGGG AGATGCCGAC GAGCCTGACG 
     5101 GTGTGGGAAT TGAAGACCCA GAAGAAGGTG AAGATCAAGA GATTCAGGTA GAAGAACCAT 
     5161 ACTATGACTG CCATGAATGC ACAGAAACCT TCACTTCCAG CACAGCATTC AGTGAACACC 
     5221 TGAAAACTCA TGCCAGCATG ATCATATTTG AGCCTGCAAA TGCCTTTGGG GAGTGCTCAG 
     5281 GCTACATCGA ACGTGCCAGC ACCAGCACAG GTGGTGCCAA TCAAGCTGAT GAGAAGTACT 
     5341 TCAAATGTGA CGTCTGTGGG CAGCTCTTCA ATGACCGCCT GTCCCTCGCC AGACACCAGA 
     5401 ATACCCACAC TGGCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5461 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5521 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5581 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5641 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5701 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5761 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5821 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5881 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5941 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6001 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6061 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6121 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6181 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6241 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6301 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6361 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6421 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6481 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6541 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6601 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6661 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6721 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6781 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6841 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6901 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6961 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7021 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7081 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7141 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7201 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7261 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7321 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7381 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7441 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7501 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7561 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7621 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7681 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7741 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7801 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7861 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7921 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7981 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8041 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8101 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8161 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8221 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8281 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8341 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8401 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8461 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8521 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8581 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8641 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8701 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8761 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8821 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8881 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8941 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9001 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9061 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9121 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9181 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9241 ATTTAACAAA AATTTAACGC GAATTTT
//