LOCUS       RC229075                8940 bp    DNA     circular  SYN 
DEFINITION  LTBP1 (Myc-DDK-tagged)-Human latent transforming growth factor beta binding protein 1 (LTBP1), transcript variant 4
ACCESSION   NM_001166264
VERSION     NM_001166264.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5097..5188 
                     /label="Myc-DDK"  
     misc_feature    5218..5803 
                     /label="PolyA signal"  
     misc_feature    5275..5294 
                     /label="Restriction Enzyme"  
     misc_feature    6079..6751 
                     /label="ColE1"  
     misc_feature    7181..7975 
                     /label="Kan/Neo"  
     misc_feature    8010..8312 
                     /label="SV40 ori"  
     misc_feature    8398..8875 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5079..5085 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5079 
                     /label="LTBP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATACTAAG CTGATGTGTT TGTTGTTCTT TTTCTCCCTG CCTCCGCTCC 
     1081 TAGTGAGTAA CCACACTGGC CGCATCAAGG TGGTCTTTAC TCCGAGCATC TGTAAAGTGA 
     1141 CCTGCACCAA GGGCAGCTGT CAGAACAGCT GTGAGAAGGG GAACACCACC ACTCTCATTA 
     1201 GTGAGAATGG TCATGCTGCC GACACCCTGA CGGCCACGAA CTTCCGAGTG GTAATTTGCC 
     1261 ATCTTCCATG TATGAATGGT GGCCAGTGCA GTTCAAGGGA CAAATGTCAG TGCCCTCCAA 
     1321 ATTTCACAGG AAAACTTTGT CAGATCCCAG TCCATGGTGC CAGCGTGCCT AAACTTTATC 
     1381 AGCATTCCCA GCAGCCAGGC AAGGCGTTGG GGACGCATGT CATCCATTCA ACACATACCT 
     1441 TGCCTCTGAC CGTGACTAGC CAGCAAGGAG TCAAAGTGAA ATTTCCTCCT AACATAGTCA 
     1501 ATATCCATGT GAAACATCCT CCTGAAGCTT CCGTCCAGAT ACATCAGGTT TCAAGAATTG 
     1561 ATGGCCCAAC AGGCCAGAAG ACAAAAGAAG CTCAACCAGG CCAATCCCAA GTCTCGTACC 
     1621 AAGGGCTTCC TGTCCAGAAG ACCCAGACCA TACATTCCAC ATACTCCCAC CAGCAGGTCA 
     1681 TTCCTCACGT CTACCCCGTG GCTGCTAAGA CACAGCTTGG CCGGTGCTTC CAGGAAACCA 
     1741 TTGGGTCACA GTGTGGCAAA GCGCTCCCTG GCCTTTCAAA GCAAGAGGAC TGCTGTGGAA 
     1801 CTGTGGGTAC CTCCTGGGGC TTTAACAAAT GCCAGAAATG CCCCAAGAAA CCATCTTATC 
     1861 ATGGATACAA CCAAATGATG GAATGCCTAC CGGGTTATAA GCGGGTTAAC AACACCTTTT 
     1921 GCCAAGATAT TAATGAATGT CAGCTACAAG GTGTATGCCC TAATGGTGAG TGTTTGAATA 
     1981 CCATGGGCAG CTATCGATGT ACCTGCAAAA TAGGATTTGG GCCGGATCCT ACCTTTTCAA 
     2041 GTTGTGTTCC TGATCCCCCT GTGATCTCGG AAGAGAAAGG GCCCTGTTAC CGACTTGTCA 
     2101 GTTCTGGAAG ACAGTGTATG CACCCTCTGT CTGTTCACCT CACCAAGCAG CTCTGCTGTT 
     2161 GTAGTGTGGG CAAGGCCTGG GGCCCACACT GTGAGAAATG TCCCCTTCCA GGCACAGCTG 
     2221 CTTTTAAGGA AATCTGTCCT GGTGGAATGG GTTATACGGT TTCTGGCGTT CATAGACGCA 
     2281 GGCCAATCCA TCACCATGTA GGTAAAGGAC CTGTATTTGT CAAGCCAAAG AACACTCAAC 
     2341 CTGTTGCTAA AAGTACTCAT CCTCCACCTC TCCCAGCCAA GGAAGAGCCA GTGGAGGCCC 
     2401 TGACCTTCTC CCGGGAACAC GGGCCAGGAG TGGCGGAGCC AGAAGTGGCA ACTGCACCCC 
     2461 CTGAAAAGGA AATACCTTCA TTGGATCAAG AGAAAACCAA ACTTGAGCCT GGTCAACCCC 
     2521 AGCTGTCTCC AGACATTTCC ACTATTCATC TGCATCCACA GTTTCCAGTA GTGATTGAAA 
     2581 AAACATCACC TCCTGTGCCT GTTGAAGTAG CTCCTGAAGC TTCTACGTCT AGTGCCAGCC 
     2641 AAGTGATTGC TCCTACTCAA GTGACAGAAA TCAATGAATG TACTGTGAAC CCTGATATCT 
     2701 GTGGAGCAGG ACACTGCATT AACCTACCAG TGAGATATAC CTGTATATGC TACGAGGGCT 
     2761 ACAGGTTCAG TGAACAACAG AGGAAATGTG TGGATATTGA TGAGTGTACT CAGGTCCAAC 
     2821 ACCTCTGCTC CCAGGGCCGC TGTGAAAACA CCGAGGGAAG TTTCTTGTGC ATTTGCCCAG 
     2881 CAGGATTTAT GGCCAGTGAG GAGGGTACTA ACTGCATAGA TGTTGACGAA TGCCTGAGGC 
     2941 CGGACGTCTG TGGGGAGGGG CACTGTGTCA ATACTGTGGG GGCCTTCCGG TGTGAATACT 
     3001 GTGACAGCGG GTACCGCATG ACTCAGAGAG GCCGTTGTGA GGATATTGAT GAATGTTTGA 
     3061 ATCCAAGCAC TTGTCCAGAT GAGCAGTGTG TGAATTCTCC TGGATCTTAC CAGTGCGTTC 
     3121 CCTGCACAGA AGGATTCCGA GGCTGGAATG GACAGTGCCT TGATGTGGAC GAGTGCCTGG 
     3181 AACCAAACGT CTGCGCAAAT GGTGATTGTT CCAACCTTGA AGGCTCCTAC ATGTGTTCAT 
     3241 GCCACAAAGG CTATACCCGG ACTCCGGACC ACAAGCACTG TAGAGATATT GATGAATGTC 
     3301 AGCAAGGGAA TCTATGTGTA AACGGGCAGT GCAAAAATAC CGAGGGCTCC TTCAGGTGCA 
     3361 CCTGTGGACA GGGGTACCAG CTGTCGGCAG CTAAAGACCA GTGTGAAGAC ATTGATGAAT 
     3421 GCCAGCACCG TCATCTCTGT GCTCATGGGC AGTGCAGGAA CACTGAGGGC TCTTTTCAAT 
     3481 GTGTGTGTGA CCAGGGTTAC AGAGCATCTG GGCTTGGAGA CCACTGTGAA GATATCAATG 
     3541 AATGCTTGGA GGACAAGAGT GTTTGCCAGA GAGGAGACTG CATTAATACT GCAGGGTCCT 
     3601 ATGATTGTAC TTGTCCGGAT GGATTTCAGC TAGATGACAA TAAAACATGT CAAGATATTA 
     3661 ATGAATGTGA ACATCCAGGG CTCTGTGGTC CGCAAGGGGA GTGCCTAAAC ACAGAGGGTT 
     3721 CTTTCCATTG TGTCTGCCAG CAGGGTTTCT CAATCTCTGC AGATGGCCGT ACGTGTGAAG 
     3781 ATGTGAATGA ATGTGAACTG CTCAGTGGGG TGTGTGGTGA AGCCTTCTGT GAAAACGTGG 
     3841 AAGGGTCCTT CCTGTGCGTG TGTGCTGATG AAAACCAAGA GTACAGCCCC ATGACTGGGC 
     3901 AGTGCCGCTC CCGGACCTCC ACAGATTTAG ATGTAGATGT AGATCAACCC AAAGAAGAAA 
     3961 AGAAAGAATG CTACTATAAT CTCAATGACG CCAGTCTCTG TGATAATGTG TTGGCCCCCA 
     4021 ATGTCACGAA ACAAGAATGC TGCTGTACAT CAGGCGCGGG ATGGGGAGAT AACTGCGAAA 
     4081 TCTTCCCCTG CCCGGTCTTG GGAACTGCTG AGTTCACTGA AATGTGTCCC AAAGGGAAAG 
     4141 GTTTTGTGCC TGCTGGAGAA TCATCTTCTG AAGCTGGTGG TGAGAACTAT AAAGATGCAG 
     4201 ATGAATGCCT ACTTTTTGGA CAAGAAATCT GCAAAAATGG TTTCTGTTTG AACACTCGGC 
     4261 CTGGGTATGA ATGCTACTGT AAGCAAGGGA CGTACTATGA TCCTGTGAAA CTGCAGTGCT 
     4321 TTGATATGGA TGAATGTCAA GACCCCAGTA GTTGTATTGA TGGCCAGTGT GTTAATACAG 
     4381 AGGGCTCTTA CAACTGCTTC TGTACTCACC CCATGGTCCT GGATGCGTCA GAAAAAAGAT 
     4441 GTATACGACC GGCTGAGTCA AACGAACAAA TAGAAGAAAC TGATGTCTAC CAAGATTTGT 
     4501 GCTGGGAACA TCTGAGTGAT GAATACGTGT GTAGCCGGCC TCTTGTGGGC AAGCAGACAA 
     4561 CGTACACTGA GTGCTGCTGT CTGTATGGAG AGGCCTGGGG CATGCAGTGT GCCCTCTGCC 
     4621 CCCTGAAGGA TTCAGATGAC TATGCTCAGC TGTGTAACAT CCCCGTGACG GGACGCCGGC 
     4681 AGCCATATGG ACGGGACGCC TTGGTTGACT TCAGTGAACA GTATACTCCA GAAGCCGATC 
     4741 CCTACTTCAT CCAAGACCGT TTTCTAAATA GCTTTGAGGA GTTACAGGCT GAGGAATGCG 
     4801 GCATCCTCAA TGGATGTGAA AATGGTCGCT GTGTGAGGGT CCAGGAAGGT TACACCTGCG 
     4861 ATTGCTTTGA TGGGTATCAC TTGGATACGG CCAAGATGAC CTGTGTCGAT GTAAATGAAT 
     4921 GCGATGAGTT GAACAACCGG ATGTCTCTCT GCAAGAATGC CAAGTGCATT AACACCGATG 
     4981 GTTCCTACAA GTGTTTGTGT CTGCCAGGCT ACGTGCCTTC TGACAAGCCA AACTACTGCA 
     5041 CTCCGTTGAA TACCGCCTTG AATTTAGAGA AAGACAGTGA CCTGGAGACG CGTACGCGGC 
     5101 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5161 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5221 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5281 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5341 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5401 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5461 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5521 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5581 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5641 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5701 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5761 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5821 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5881 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5941 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6001 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6061 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6121 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6181 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6241 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6301 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6361 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6421 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6481 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6541 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6601 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6661 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6721 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6781 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6841 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6901 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6961 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7021 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7081 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7141 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7201 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7261 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7321 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7381 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7441 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7501 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7561 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7621 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7681 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7741 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7801 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7861 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7921 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7981 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8041 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8101 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8161 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8221 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8281 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8341 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8401 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8461 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8521 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8581 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8641 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8701 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8761 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8821 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8881 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//