LOCUS       RC229074                8865 bp    DNA     circular  SYN 
DEFINITION  ACIN1 (Myc-DDK-tagged)-Human apoptotic chromatin condensation inducer 1 (ACIN1), transcript variant 2
ACCESSION   NM_001164814
VERSION     NM_001164814.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5022..5113 
                     /label="Myc-DDK"  
     misc_feature    5143..5728 
                     /label="PolyA signal"  
     misc_feature    5200..5219 
                     /label="Restriction Enzyme"  
     misc_feature    6004..6676 
                     /label="ColE1"  
     misc_feature    7106..7900 
                     /label="Kan/Neo"  
     misc_feature    7935..8237 
                     /label="SV40 ori"  
     misc_feature    8323..8800 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5004..5010 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5004 
                     /label="ACIN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGAGACGG AAACATCCGA GGACATCCGG AGGAACCCGG GGAGTTCTGA 
     1081 GTGGTAATCG AGGGGTAGAG TATGGCAGTG GGCGGGGTCA TCTCGGTACG TTCGAAGGGC 
     1141 GTTGGCGGAA ATTACCGAAG ATGCCCGAAG CCGTCGGGAC GGACCCGAGT ACCTCACGCA 
     1201 AGATGGCGGA GCTGGAGGAG GTGACTCTGG ACGGGAAGCC TCTTCAGGCG CTGCGGGTGA 
     1261 CCGACCTGAA GGCCGCACTG GAGCAGCGAG GCCTAGCCAA GAGCGGGCAG AAGAGTGCCC 
     1321 TGGTCAAGCG GCTCAAAGGG GCTCTAATGC TAGAAAATTT ACAGAAACAC TCAACACCCC 
     1381 ATGCTGCATT CCAGCCAAAT TCCCAGATTG GTGAGGAAAT GAGCCAGAAC AGTTTCATAA 
     1441 AACAGTATCT GGAAAAGCAG CAGGAGCTAC TTAGGCAGCG TCTGGAACGT GAAGCTCGAG 
     1501 AAGCTGCAGA ACTTGAAGAA GCTTCAGCTG AGTCGGAGGA CGAGATGATC CATCCTGAGG 
     1561 GAGTGGCTTC CCTGCTGCCT CCTGACTTTC AGAGCAGCCT GGAGAGACCA GAGCTGGAGC 
     1621 TCAGCAGACA TTCGCCCAGA AAAAGCTCCT CAATTTCTGA AGAGAAAGGT GACTCTGATG 
     1681 ATGAGAAACC AAGGAAAGGA GAAAGACGAT CATCTAGGGT CAGACAGGCA AGAGCAGCTA 
     1741 AACTGTCTGA GGGCAGCCAA CCTGCTGAGG AGGAAGAGGA TCAAGAAACA CCTTCCAGAA 
     1801 ACCTAAGGGT CAGAGCAGAT CGAAATTTGA AAACAGAGGA GGAAGAAGAG GAGGAGGAGG 
     1861 AGGAGGAAGA AGATGATGAA GAAGAGGAAG GTGATGATGA GGGACAAAAA TCTAGGGAGG 
     1921 CACCAATCCT GAAAGAGTTT AAGGAAGAAG GGGAAGAGAT ACCTAGAGTA AAACCAGAGG 
     1981 AGATGATGGA TGAGAGACCC AAAACAAGAT CCCAGGAACA GGAGGTGTTA GAGAGAGGAG 
     2041 GGAGATTTAC AAGATCCCAG GAAGAGGCTA GAAAAAGTCA TCTGGCCAGA CAGCAGCAGG 
     2101 AGAAGGAAAT GAAAACAACA TCTCCCCTTG AGGAGGAAGA AAGAGAAATA AAATCTTCAC 
     2161 AAGGCTTAAA GGAAAAATCG AAGTCTCCTT CCCCTCCTCG ACTGACTGAA GATCGAAAGA 
     2221 AGGCCTCACT TGTAGCGCTG CCAGAGCAAA CTGCCAGCGA GGAGGAGACT CCTCCACCTT 
     2281 TACTAACAAA GGAAGCATCT TCTCCACCAC CTCATCCACA GCTCCATAGC GAAGAAGAAA 
     2341 TAGAGCCCAT GGAAGGCCCA GCCCCCCCTG TCCTCATTCA GTTATCTCCT CCTAATACAG 
     2401 ATGCTGACAC CAGGGAGCTA TTAGTATCTC AGCATACTGT CCAGTTGGTA GGAGGCCTGT 
     2461 CTCCTTTGTC AAGTCCTTCA GACACCAAAG CAGAATCTCC AGCAGAGAAA GTGCCAGAGG 
     2521 AGAGTGTCCT GCCTCTGGTT CAGAAAAGCA CACTGGCTGA CTACTCAGCC CAGAAGGATC 
     2581 TTGAACCTGA GTCAGACAGA TCTGCTCAGC CCCTCCCTCT AAAAATTGAG GAATTAGCAC 
     2641 TGGCCAAAGG AATCACTGAA GAATGTCTGA AACAGCCATC TTTGGAACAG AAGGAAGGCA 
     2701 GAAGAGCTTC TCATACCCTT CTCCCAAGCC ACAGATTGAA ACAGTCAGCT GATTCATCCT 
     2761 CTAGCCGGTC CTCCTCATCT TCCTCCTCCA GTTCTAGATC AAGATCTCGC TCTCCTGACA 
     2821 GTTCAGGTTC TCGGTCTCAT TCACCGCTCA GATCCAAGCA GAGAGATGTA GCCCAGGCAC 
     2881 GTACTCATGC CAACCCTCGT GGTAGACCCA AGATGGGCTC CAGATCAACA TCAGAGTCCA 
     2941 GATCAAGGTC ACGTTCACGT TCTCGTTCAG CATCAAGCAA CAGCAGAAAA TCTCTGAGCC 
     3001 CTGGAGTCTC CAGGGACAGC AGCACCAGCT ATACTGAAAC CAAAGATCCC TCTTCTGGTC 
     3061 AGGAGGTTGC AACTCCACCA GTGCCACAAC TGCAGGTCTG TGAGCCAAAG GAGAGGACTT 
     3121 CCACCTCCTC ATCCTCTGTC CAAGCAAGGC GTCTGAGTCA GCCTGAATCA GCTGAAAAGC 
     3181 ATGTGACCCA GAGGTTACAG CCTGAGCGGG GGAGCCCAAA GAAGTGTGAA GCTGAAGAGG 
     3241 CAGAGCCACC AGCTGCCACA CAGCCCCAAA CCTCAGAGAC TCAGACCTCT CATCTGCCAG 
     3301 AATCAGAAAG AATTCATCAC ACTGTTGAGG AGAAGGAGGA AGTGACCATG GACACAAGTG 
     3361 AAAACAGACC TGAAAATGAT GTTCCAGAAC CTCCCATGCC TATTGCAGAC CAAGTCAGCA 
     3421 ATGATGACCG CCCGGAGGGC AGTGTTGAAG ATGAGGAGAA GAAAGAGAGC TCGCTGCCCA 
     3481 AATCATTCAA GAGGAAGATC TCCGTTGTCT CTACCAAGGG GGTGCCAGCT GGAAACAGTG 
     3541 ACACAGAGGG GGGCCAGCCT GGTCGGAAAC GACGCTGGGG AGCCAGCACA GCCACCACAC 
     3601 AGAAGAAACC TTCCATCAGT ATCACCACTG AATCACTAAA GGAGGCTGTT GTGGATCTTC 
     3661 ATGCTGATGA CTCTCGCATC TCTGAGGATG AGACAGAGCG TAATGGCGAT GATGGGACCC 
     3721 ATGACAAGGG GCTGAAAATA TGCCGGACAG TCACTCAGGT AGTACCTGCA GAGGGCCAGG 
     3781 AGAATGGGCA GAGGGAAGAA GAGGAAGAAG AGAAGGAACC TGAAGCAGAA CCTCCTGTAC 
     3841 CTCCCCAGGT GTCAGTAGAG GTGGCCTTGC CCCCACCTGC AGAGCATGAA GTAAAGAAAG 
     3901 TGACTTTAGG AGATACCTTA ACTCGACGTT CCATTAGCCA GCAGAAGTCC GGAGTTTCCA 
     3961 TTACCATTGA TGACCCAGTC CGAACTGCCC AGGTGCCCTC CCCACCCCGG GGCAAGATTA 
     4021 GCAACATTGT CCATATCTCC AATTTGGTCC GTCCTTTCAC TTTAGGCCAG CTAAAGGAGT 
     4081 TGTTGGGGCG CACAGGAACC TTGGTGGAAG AGGCCTTCTG GATTGACAAG ATCAAATCTC 
     4141 ATTGCTTTGT AACGTACTCA ACAGTAGAGG AAGCTGTTGC CACCCGCACA GCTCTGCACG 
     4201 GGGTCAAATG GCCCCAGTCC AATCCCAAAT TCCTTTGTGC TGACTATGCC GAGCAAGATG 
     4261 AGCTGGATTA TCACCGAGGC CTCTTGGTGG ACCGTCCCTC TGAAACTAAG ACAGAGGAGC 
     4321 AGGGAATACC ACGGCCCCTG CACCCCCCAC CCCCACCCCC GGTCCAGCCA CCACAGCACC 
     4381 CCCGGGCAGA GCAGCGGGAG CAGGAACGGG CAGTGCGGGA ACAGTGGGCA GAACGGGAAC 
     4441 GGGAAATGGA GCGGCGGGAG CGGACTCGAT CAGAGCGTGA ATGGGATCGG GACAAAGTTC 
     4501 GAGAAGGGCC CCGTTCCCGA TCAAGGTCCC GTGACCGCCG CCGCAAGGAA CGTGCGAAGT 
     4561 CTAAAGAAAA GAAGAGTGAG AAGAAAGAGA AAGCCCAGGA GGAACCACCT GCCAAGCTGC 
     4621 TGGATGACCT TTTCCGAAAG ACCAAGGCAG CTCCCTGCAT CTATTGGCTC CCACTGACTG 
     4681 ACAGCCAGAT CGTTCAGAAA GAGGCAGAGC GGGCCGAACG GGCCAAGGAG CGGGAGAAGC 
     4741 GGCGAAAGGA GCAAGAAGAA GAAGAGCAAA AGGAGCGGGA GAAGGAAGCC GAGCGGGAAC 
     4801 GGAACCGACA GCTGGAGCGA GAGAAACGTC GGGAGCACAG TCGGGAGAGG GACAGGGAGA 
     4861 GAGAGAGAGA AAGGGAGCGG GACAGGGGGG ACCGAGATCG GGATAGGGAA AGGGACCGAG 
     4921 AACGAGGCAG GGAAAGGGAT CGCAGGGACA CCAAGCGCCA CAGCAGAAGC CGGAGTCGGA 
     4981 GCACACCTGT GCGGGACCGG GGTGGGCGCC GCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5041 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5101 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5161 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5221 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5281 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5341 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5401 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5461 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5521 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5581 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5641 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5701 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5761 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5821 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5881 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5941 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6001 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6061 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6121 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6181 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6241 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6301 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6361 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6421 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6481 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6541 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6601 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6661 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6721 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6781 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6841 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6901 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6961 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7021 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7081 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7141 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7201 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7261 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7321 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7381 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7441 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7501 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7561 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7621 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7681 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7741 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7801 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7861 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7921 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7981 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8041 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8101 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8161 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8221 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8281 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8341 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8401 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8461 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8521 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8581 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8641 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8701 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8761 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8821 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//