LOCUS       RC229069                8589 bp    DNA     circular  SYN 
DEFINITION  SYNRG (Myc-DDK-tagged)-Human synergin, gamma (SYNRG), transcript variant 4
ACCESSION   NM_001163544
VERSION     NM_001163544.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4746..4837 
                     /label="Myc-DDK"  
     misc_feature    4867..5452 
                     /label="PolyA signal"  
     misc_feature    4924..4943 
                     /label="Restriction Enzyme"  
     misc_feature    5728..6400 
                     /label="ColE1"  
     misc_feature    6830..7624 
                     /label="Kan/Neo"  
     misc_feature    7659..7961 
                     /label="SV40 ori"  
     misc_feature    8047..8524 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4728..4734 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4728 
                     /label="SYNRG"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGCGG CCAGGAGCTG GTTCTGGTGG CGGCGGGGCC GCGGGAGCTG 
     1081 GCGCGGGGTC CGCCGGGGGA GGCGGCTTCA TGTTTCCTGT TGCAGGTGGG ATAAGACCCC 
     1141 CTCAAGCAGG CCTGATGCCG ATGCAGCAAC AAGGATTTCC TATGGTCTCT GTCATGCAGC 
     1201 CTAATATGCA AGGCATTATG GGAATGAATT ACAGCTCTCA GATGTCCCAA GGACCTATTG 
     1261 CTATGCAGGC AGGAATACCA ATGGGACCAA TGCCAGCAGC GGGAATGCCT TACCTAGGAC 
     1321 AAGCACCCTT CCTGGGCATG CGTCCTCCAG GCCCACAGTA CACTCCAGAC ATGCAGAAGC 
     1381 AGTTTGCCGA AGAGCAGCAG AAACGATTTG AACAGCAGCA AAAACTCTTA GAAGAAGAAA 
     1441 GAAAAAGACG CCAGTTTGAA GAGCAGAAGC AAAAGCTCAG ACTTTTGAGC AGTGTGAAAC 
     1501 CCAAGACAGG AGAGAAGAGT AGAGATGATG CTTTGGAAGC CATAAAAGGA AATTTAGATG 
     1561 GGTTTTCCAG AGATGCAAAA ATGCACCCTA CTCCAGCATC GCACCCCAAG AAACCAGGTG 
     1621 TGGGAGTATT TCCCTCACAG GATCCTGCTC AGCCCAGAAT GCCTCCTTGG ATTTACAATG 
     1681 AGAGTTTGGT TCCAGATGCC TATAAGAAAA TCTTAGAAAC CACAATGACT CCAACTGGAA 
     1741 TAGATACTGC CAAACTGTAT CCCATTCTGA TGTCATCTGG GCTTCCCAGG GAAACTCTTG 
     1801 GACAGATATG GGCCTTAGCT AATCGAACTA CACCTGGCAA ACTTACAAAA GAAGAACTTT 
     1861 ATACCGTTCT AGCCATGATA GCGGTAACAC AGAGGGGCGT TCCTGCAATG AGTCCTGATG 
     1921 CTTTAAACCA GTTCCCAGCA GCTCCTATTC CAACTTTAAG TGGCTTTTCT ATGACTCTGC 
     1981 CTACACCGGT GAGTCAGCCA ACTGTGATAC CTTCAGGTCC TGCGGGCTCC ATGCCCCTCA 
     2041 GCCTTGGACA GCCAGTCATG GGCATTAACC TTGTTGGACC AGTGGGTGGA GCTGCAGCCC 
     2101 AGGCTTCTAG TGGTTTCATA CCAACCTACC CTGCAAATCA GGTAGTAAAG CCAGAAGAAG 
     2161 ATGACTTCCA GGATTTTCAA GATGCTTCTA AGTCAGGATC CCTTGATGAC TCATTCAGTG 
     2221 ATTTCCAAGA GTTGCCTGCT TCTTCAAAAA CAAGTAACTC CCAGCATGGA AACAGTGCCC 
     2281 CTTCTTTGTT GATGCCACTT CCTGGAACTA AAGCATTGCC TTCAATGGAC AAATATGCTG 
     2341 TGTTTAAAGG AATTGCAGCT GACAAGTCCT CTGAAAATAC TGTTCCACCT GGAGATCCTG 
     2401 GTGATAAATA TAGTGCTTTC AGAGAACTTG AACAGACAGC AGAGAATAAA CCTTTAGGAG 
     2461 AAAGCTTTGC AGAATTCAGA TCTGCAGGAA CTGATGATGG TTTCACCGAT TTTAAAACAG 
     2521 CCGATAGTGT ATCACCACTA GAGCCACCAA CAAAAGACAA AACTTTTCCA CCATCCTTCC 
     2581 CCTCAGGAAC TATACAACAG AAACAACAAA CACAAGTGAA AAACCCTCTG AACTTAGCAG 
     2641 ACCTAGATAT GTTTTCCTCA GTTAATTGCA GCAGCGAGAA ACCATTGTCT TTTTCAGCTG 
     2701 TGTTTAGCAC ATCAAAATCA GTTTCTACAC CACAGTCAAC AGGTTCTGCT GCTACTATGA 
     2761 CAGCATTGGC AGCAACAAAA ACTTCTAGTT TGGCTGATGA TTTTGGAGAA TTCAGCCTTT 
     2821 TTGGGGAATA TTCTGGTCTA GCACCTGTTG GGGAGCAGGA TGACTTTGCA GATTTTATGG 
     2881 CTTTCAGTAA TAGCTCTATT TCATCTGAGC AAAAGCCGGA TGACAAATAT GATGCCCTTA 
     2941 AAGAGGAAGC CAGTCCTGTT CCTCTAACCA GCAACGTGGG CAGCACAGTG AAGGGTGGAC 
     3001 AAAACTCGAC TGCTGCGTCT ACCAAGTACG ATGTCTTCAG ACAACTTTCT CTGGAAGGGT 
     3061 CTGGACTAGG TGTTGAAGAC CTGAAAGATA ACACTCCTTC AGGAAAAAGT GATGATGATT 
     3121 TTGCTGACTT CCACTCCAGT AAATTTTCTT CCATAAACTC GGACAAATCC CTGGGAGAGA 
     3181 AAGCAGTGGC TTTCAGACAC ACCAAAGAAG ACTCTGCATC AGTGAAGTCC TTAGATCTCC 
     3241 CTTCCATTGG TGGCAGCAGT GTTGGCAAGG AGGACTCTGA AGATGCACTC TCTGTTCAGT 
     3301 TTGACATGAA ATTGGCTGAT GTGGGAGGAG ATCTTAAGCA TGTCATGTCT GATAGCTCTT 
     3361 TGGATTTACC AACAGTTAGT GGCCAGCATC CTCCTGCTGC AGATATAGAG GACTTAAAAT 
     3421 ATGCTGCTTT TGGAAGCTAC AGTAGCAATT TTGCAGTGAG CACACTTACA AGCTATGACT 
     3481 GGTCAGACAG GGATGATGCA ACTCAGGGCA GAAAACTCTC TCCATTTGTC CTCTCAGCAG 
     3541 GAAGTGGATC CCCCTCAGCC ACCTCAATTC TTCAAAAGAA AGAGACTTCA TTTGGCAGTT 
     3601 CTGAAAACAT CACCATGACA TCTCTCTCCA AAGTAACGAC CTTTGTAAGT GAAGATGCTC 
     3661 TTCCAGAGAC CACCTTCCCA GCTCTTGCCA GTTTTAAAGA CACGATTCCT CAGACCAGTG 
     3721 AGCAAAAGGA ATATGAAAAC AGAGACTATA AAGATTTCAC AAAACAGGAC CTGCCTACGG 
     3781 CTGAACGGAG CCAGGAGGCC ACGTGTCCCA GCCCAGCGTC CAGTGGTGCC TCTCAAGAAA 
     3841 CCCCGAACGA ATGTTCGGAT GACTTTGGAG AGTTTCAAAG TGAAAAGCCC AAAATCAGCA 
     3901 AATTTGACTT CTTAGTAGCC ACTTCACAAA GCAAAATGAA ATCCAGTGAA GAAATGATCA 
     3961 AAAGTGAGCT GGCAACCTTT GACCTTTCTG TTCAAGGATC ACACAAGAGG AGTTTGAGCC 
     4021 TTGGTGATAA AGAAATAAGC CGTTCTTCTC CTTCTCCAGC TTTGGAGCAG CCTTTCAGAG 
     4081 ACCGTTCCAA TACTCTGAAT GAGAAGCCCG CCCTGCCCGT CATCCGAGAC AAGTACAAAG 
     4141 ACCTGACGGG AGAGGTGGAG GAAAATGAGA GATATGCATA TGAATGGCAG AGATGCCTGG 
     4201 GGAGTGCCCT GAATGTCATT AAGAAGGCAA ATGATACCTT AAATGGAATC AGTAGTAGTT 
     4261 CTGTTTGCAC AGAAGTAATT CAGTCAGCTC AAGGCATGGA ATATTTATTA GGTGTTGTTG 
     4321 AAGTGTACAG GGTAACCAAG CGTGTGGAGC TGGGGATAAA AGCCACTGCA GTGTGCAGTG 
     4381 AGAAACTCCA GCAGTTGCTG AAGGACATCG ATAAAGTATG GAATAACCTA ATCGGCTTCA 
     4441 TGTCACTCGC CACACTCACA CCAGATGAAA ACTCGCTGGA TTTTTCCTCC TGTATGTTAC 
     4501 GGCCTGGGAT TAAAAATGCT CAGGAGCTTG CCTGTGGAGT GTGCCTCTTG AATGTGGACT 
     4561 CGAGGAGCCG GAAAGAAGAG AAGCCTGCAG AAGAACATCC TAAAAAAGCA TTCAACTCAG 
     4621 AAACAGACAG TTTCAAGCTG GCCTATGGAG GGCACCAGTA TCACGCCAGC TGTGCCAACT 
     4681 TCTGGATCAA CTGTGTCGAA CCAAAGCCTC CTGGCCTCGT CCTGCCTGAC CTGCTCACGC 
     4741 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4801 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4861 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4921 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4981 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5041 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5101 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5161 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5221 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5281 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5341 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5401 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5461 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5521 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5581 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5641 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5701 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5761 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5821 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5881 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5941 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6001 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6061 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6121 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6181 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6241 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6301 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6361 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6421 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6481 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6541 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6601 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6661 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6721 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6781 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6841 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6901 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6961 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7021 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7081 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7141 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7201 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7261 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7321 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7381 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7441 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7501 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7561 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7621 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7681 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7741 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7801 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7861 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7921 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7981 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8041 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8101 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8161 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8221 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8281 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8341 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8401 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8461 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8521 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8581 GCGAATTTT
//